LOCUS       RC210800                8409 bp    DNA     circular  SYN 
DEFINITION  UBE4B (Myc-DDK-tagged)-Human ubiquitination factor E4B (UBE4B), transcript variant 2
ACCESSION   NM_006048
VERSION     NM_006048.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4564..4655 
                     /label="Myc-DDK"  
     misc_feature    4685..5270 
                     /label="PolyA signal"  
     misc_feature    4742..4761 
                     /label="Restriction Enzyme"  
     misc_feature    5546..6218 
                     /label="ColE1"  
     misc_feature    6648..7442 
                     /label="Kan/Neo"  
     misc_feature    7477..7779 
                     /label="SV40 ori"  
     misc_feature    7865..8342 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4539..4546 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4539 
                     /label="UBE4B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGAGCTG AGCGCTGATG AGATTCGACG GAGGCGCCTT GCACGACTTG 
     1081 CTGGTGGACA GACCTCTCAG CCAACCACCC CACTCACCTC TCCCCAGAGG GAGAACCCTC 
     1141 CGGGGCCTCC CATAGCGGCA TCAGCCCCAG GACCCTCTCA GAGTCTTGGT CTCAATGTCC 
     1201 ACAACATGAC CCCAGCTACC TCCCCAATAG GTGCATCAGG AGTAGCCCAT CGAAGCCAGA 
     1261 GCAGTGAAGG AGTCAGTTCT CTCAGCAGCT CGCCCTCTAA TAGCCTTGAA ACGCAATCTC 
     1321 AGTCTCTCTC ACGTTCCCAG AGCATGGATA TCGATGGTGT CTCATGTGAG AAAAGCATGT 
     1381 CCCAGGTGGA TGTGGATTCA GGAATTGAAA ACATGGAGGT TGATGAAAAT GATCGAAGAG 
     1441 AAAAGCGGAG CCTCAGTGAT AAGGAGCCTT CCTCGGGCCC TGAAGTGTCT GAAGAGCAGG 
     1501 CCTTACAGCT GGTCTGTAAG ATCTTCCGTG TCTCTTGGAA GGACCGGGAC AGAGATGTCA 
     1561 TCTTTCTTTC TTCTCTTTCT GCACAGTTTA AGCAGAACCC AAAAGAAGTA TTCTCCGATT 
     1621 TTAAGGACTT GATTGGCCAG ATTTTAATGG AAGTGCTAAT GATGTCCACT CAGACCAGAG 
     1681 ATGAAAACCC ATTTGCCAGT CTGACAGCCA CATCACAGCC AATTGCTGCA GCAGCACGGT 
     1741 CACCAGACAG AAATCTCTTG CTAAACACTG GCTCCAATCC AGGAACAAGC CCCATGTTCT 
     1801 GCAGCGTGGC TTCCTTTGGT GCCAGCTCTT TGTCTAGTTT GGGAGCCTCT GGTGGAGCAA 
     1861 GTAATTGGGA TTCCTACAGT GACCATTTCA CCATTGAAAC CTGCAAAGAG ACAGATATGC 
     1921 TGAACTACCT CATCGAGTGT TTCGACCGAG TTGGAATAGA GGAAAAAAAA GCACCAAAGA 
     1981 TGTGCAGCCA GCCAGCAGTC AGCCAGCTTC TGAGCAACAT CCGCTCACAG TGCATATCCC 
     2041 ATACTGCTTT AGTACTACAA GGCTCCCTAA CACAGCCCAG GTCCTTGCAG CAGCCGTCCT 
     2101 TCCTAGTGCC GTATATGCTG TGTAGGAATC TCCCATATGG CTTCATTCAG GAACTGGTGA 
     2161 GAACCACTCA CCAGGATGAA GAAGTGTTCA AGCAGATATT TATCCCCATT TTACAAGGCC 
     2221 TGGCTCTTGC TGCCAAAGAG TGCTCCCTCG ACAGTGACTA CTTTAAATAC CCCCTCATGG 
     2281 CACTAGGTGA GCTCTGTGAA ACCAAGTTTG GGAAGACACA CCCTGTGTGC AATTTGGTTG 
     2341 CTTCTTTGCG GTTGTGGTTG CCGAAATCCT TAAGTCCTGG CTGTGGGCGG GAGCTGCAGA 
     2401 GACTCTCTTA CTTAGGGGCT TTCTTTAGCT TCTCAGTCTT TGCAGAAGAT GATGTTAAAG 
     2461 TGGTTGAAAA ATACTTCTCA GGGCCTGCCA TTACCCTGGA AAACACTCGT GTGGTTAGCC 
     2521 AATCATTGCA GCATTACTTA GAGCTCGGAA GGCAAGAGCT TTTTAAGATT CTGCATAGTA 
     2581 TTTTGTTAAA TGGCGAAACC CGTGAGGCTG CTCTCAGTTA CATGGCGGCT GTCGTCAATG 
     2641 CCAATATGAA GAAAGCACAG ATGCAGACAG ATGATAGATT GGTGTCTACA GATGGATTTA 
     2701 TGCTGAATTT CCTTTGGGTA CTGCAGCAGC TAAGTACAAA AATCAAGTTA GAAACAGTTG 
     2761 ATCCCACGTA TATTTTTCAC CCAAGATGTC GGATTACTCT TCCCAATGAT GAGACGCGTG 
     2821 TGAATGCAAC GATGGAAGAT GTGAATGACT GGCTGACTGA ACTCTATGGC GATCAGCCTC 
     2881 CATTTTCTGA GCCGAAATTC CCTACGGAGT GCTTCTTTCT CACCCTGCAT GCTCACCACC 
     2941 TCTCTATTCT GCCTAGTTGC CGTCGCTATA TCCGCAGACT CCGGGCTATC CGGGAGCTCA 
     3001 ATAGAACTGT AGAAGATTTG AAAAATAATG AAAGCCAATG GAAAGATTCC CCACTGGCAA 
     3061 CTAGACACCG CGAAATGCTG AAGCGCTGTA AAACTCAGCT TAAGAAACTG GTACGGTGCA 
     3121 AGGCCTGTGC TGATGCTGGC CTACTTGACG AGAGCTTCCT GAGAAGATGT CTGAATTTTT 
     3181 ATGGCCTTCT CATTCAGCTG CTGCTCCGCA TCCTGGACCC CGCATATCCC GATATAACAC 
     3241 TGCCTTTAAA TTCAGATGTC CCCAAGGTAT TTGCAGCGTT GCCTGAGTTT TATGTAGAAG 
     3301 ATGTTGCAGA ATTTTTATTT TTTATTGTAC AATACTCTCC CCAGGCGCTT TATGAGCCCT 
     3361 GTACTCAGGA TATTGTGATG TTCCTTGTTG TGATGTTGTG CAACCAGAAC TACATCCGAA 
     3421 ACCCATATTT GGTGGCCAAA CTGGTAGAAG TCATGTTTAT GACCAACCCT GCTGTTCAGC 
     3481 CACGAACCCA GAAGTTTTTT GAAATGATTG AGAACCATCC TCTCTCCACC AAGTTGTTGG 
     3541 TACCTTCCCT GATGAAGTTT TATACAGATG TTGAGCATAC CGGAGCCACC AGTGAGTTTT 
     3601 ATGACAAGTT CACAATTCGC TATCATATTA GCACCATTTT TAAAAGCCTT TGGCAAAACA 
     3661 TAGCTCACCA TGGCACCTTT ATGGAGGAGT TCAACTCCGG GAAGCAGTTT GTTCGCTATA 
     3721 TAAACATGTT GATAAACGAC ACGACGTTTT TGCTCGATGA AAGTCTGGAG TCTCTGAAGC 
     3781 GAATCCATGA AGTGCAGGAA GAGATGAAGA ACAAAGAACA GTGGGACCAG TTGCCCCGGG 
     3841 ATCAGCAGCA GGCTCGTCAG TCTCAGCTTG CTCAGGATGA GCGTGTGTCC CGCTCTTACC 
     3901 TCGCCCTGGC CACCGAAACC GTGGACATGT TCCACATCCT CACGAAGCAG GTCCAGAAGC 
     3961 CCTTCCTCAG ACCGGAGCTT GGACCCCGAT TGGCTGCAAT GCTGAACTTT AATCTTCAGC 
     4021 AACTTTGTGG CCCCAAGTGC CGTGACCTGA AAGTTGAAAA CCCTGAGAAA TACGGCTTTG 
     4081 AACCAAAGAA GCTGTTGGAC CAACTGACGG ATATTTACTT ACAGCTGGAC TGTGCTCGGT 
     4141 TCGCGAAAGC CATTGCTGAC GACCAGAGAT CCTACAGTAA GGAATTGTTT GAAGAAGTTA 
     4201 TTTCAAAGAT GCGGAAGGCA GGGATCAAAT CCACAATAGC AATAGAAAAA TTTAAGCTGC 
     4261 TCGCCGAGAA AGTGGAGGAG ATAGTGGCCA AGAACGCACG CGCAGAAATC GACTACAGCG 
     4321 ACGCTCCTGA TGAGTTCAGA GACCCTCTGA TGGACACCCT CATGACAGAC CCCGTGCGGC 
     4381 TGCCCTCTGG CACCATCATG GACCGCTCCA TCATCCTGCG GCACCTGCTC AACTCCCCCA 
     4441 CGGACCCCTT CAACCGGCAG ACGCTGACAG AGAGCATGCT GGAACCAGTG CCAGAACTGA 
     4501 AAGAGCAGAT TCAGGCGTGG ATGAGAGAGA AACAGAACAG CGATCACAGC GGACCGACGC 
     4561 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4621 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4681 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4741 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4801 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4861 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4921 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4981 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5041 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5101 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5161 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5221 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5281 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5341 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5401 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5461 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5521 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5581 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5641 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5701 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5761 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5821 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5881 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5941 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6001 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6061 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6121 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6181 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6241 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6301 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6361 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6421 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6481 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6541 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6601 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6661 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6721 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6781 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6841 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6901 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6961 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7021 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7081 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7141 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7201 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7261 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7321 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7381 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7441 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7501 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7561 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7621 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7681 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7741 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7801 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7861 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7921 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7981 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8041 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8101 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8161 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8221 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8281 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8341 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8401 GCGAATTTT
//