LOCUS       RC210760                7638 bp    DNA     circular  SYN 
DEFINITION  LIG1 (Myc-DDK-tagged)-Human ligase I, DNA, ATP-dependent (LIG1)
ACCESSION   NM_000234
VERSION     NM_000234.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3795..3886 
                     /label="Myc-DDK"  
     misc_feature    3916..4501 
                     /label="PolyA signal"  
     misc_feature    3973..3992 
                     /label="Restriction Enzyme"  
     misc_feature    4777..5449 
                     /label="ColE1"  
     misc_feature    5879..6673 
                     /label="Kan/Neo"  
     misc_feature    6708..7010 
                     /label="SV40 ori"  
     misc_feature    7096..7573 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3777..3783 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3777 
                     /label="LIG1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGCGAAGT ATCATGTCAT TTTTCCACCC CAAGAAAGAG GGTAAAGCAA 
     1081 AGAAGCCTGA GAAGGAGGCA TCCAATAGCA GCAGAGAGAC GGAGCCCCCT CCAAAGGCGG 
     1141 CACTGAAGGA GTGGAATGGA GTGGTGTCCG AGAGTGACTC TCCGGTGAAG AGGCCAGGGA 
     1201 GGAAGGCGGC CCGGGTCCTG GGCAGCGAAG GGGAAGAGGA GGATGAAGCC CTTAGCCCTG 
     1261 CTAAAGGCCA GAAGCCTGCC CTGGACTGCT CACAGGTCTC CCCGCCCCGT CCTGCCACAT 
     1321 CTCCTGAGAA CAATGCTTCC CTCTCTGACA CCTCTCCCAT GGACAGTTCC CCATCAGGGA 
     1381 TTCCGAAGCG TCGCACAGCT CGGAAGCAGC TCCCGAAACG GACCATTCAG GAAGTCCTGG 
     1441 AAGAGCAGAG TGAGGACGAG GACAGAGAAG CCAAGAGGAA GAAGGAGGAG GAAGAAGAGG 
     1501 AGACCCCGAA AGAAAGCCTC ACAGAGGCTG AAGTGGCCAC AGAGAAGGAA GGAGAAGACG 
     1561 GGGACCAGCC CACCACGCCT CCCAAGCCCC TAAAGACCTC CAAAGCAGAG ACCCCGACGG 
     1621 AAAGCGTTTC AGAGCCTGAG GTGGCCACGA AGCAGGAACT GCAGGAGGAG GAAGAGCAGA 
     1681 CCAAGCCTCC CCGCAGAGCT CCCAAGACGC TCAGCAGCTT CTTCACCCCC CGGAAGCCAG 
     1741 CAGTCAAAAA AGAAGTGAAG GAAGAGGAGC CAGGGGCTCC AGGAAAGGAG GGAGCTGCTG 
     1801 AGGGACCCCT GGATCCATCT GGTTACAATC CTGCCAAGAA CAACTATCAT CCCGTGGAAG 
     1861 ATGCCTGCTG GAAACCGGGC CAGAAGGTTC CTTACCTGGC TGTGGCCCGG ACGTTTGAGA 
     1921 AGATCGAGGA GGTGTCTGCT CGGCTCCGGA TGGTGGAGAC GCTGAGCAAC TTGCTGCGCT 
     1981 CCGTGGTGGC CCTGTCGCCT CCAGACCTCC TCCCTGTCCT CTACCTCAGC CTCAACCACC 
     2041 TTGGGCCACC CCAGCAGGGC CTGGAGCTTG GCGTGGGTGA TGGTGTCCTT CTCAAGGCAG 
     2101 TGGCCCAGGC CACAGGTCGG CAGCTGGAGT CCGTCCGGGC TGAGGCAGCC GAGAAAGGCG 
     2161 ACGTGGGGCT GGTGGCCGAG AACAGCCGCA GCACCCAGAG GCTCATGCTG CCACCACCTC 
     2221 CGCTCACTGC CTCCGGGGTC TTCAGCAAGT TCCGCGACAT CGCCAGGCTC ACTGGCAGTG 
     2281 CTTCCACAGC CAAGAAGATA GACATCATCA AAGGCCTCTT TGTGGCCTGC CGCCACTCAG 
     2341 AAGCCCGGTT CATCGCTAGG TCCCTGAGCG GACGGCTGCG CCTTGGGCTG GCAGAGCAGT 
     2401 CGGTGCTGGC TGCCCTCTCC CAGGCAGTGA GCCTCACGCC CCCGGGCCAA GAATTCCCAC 
     2461 CAGCCATGGT GGATGCTGGG AAGGGCAAGA CAGCAGAGGC CAGAAAGACG TGGCTGGAGG 
     2521 AGCAAGGCAT GATCCTGAAG CAGACGTTCT GCGAGGTTCC CGACCTGGAC CGAATTATCC 
     2581 CCGTGCTGCT GGAGCACGGC CTGGAACGTC TCCCGGAGCA CTGCAAGCTG AGCCCAGGGA 
     2641 TTCCCCTGAA ACCAATGTTG GCCCATCCCA CCCGGGGCAT CAGCGAGGTC CTGAAACGCT 
     2701 TTGAGGAGGC AGCTTTCACC TGCGAATACA AATATGACGG GCAGAGGGCA CAGATCCACG 
     2761 CCCTGGAAGG CGGGGAGGTG AAGATCTTCA GCAGGAATCA GGAAGACAAC ACTGGGAAGT 
     2821 ACCCGGACAT CATCAGCCGC ATCCCCAAGA TTAAACTCCC ATCGGTCACA TCCTTCATCC 
     2881 TGGACACCGA AGCCGTGGCT TGGGACCGGG AAAAGAAGCA GATCCAGCCA TTCCAAGTGC 
     2941 TCACCACCCG CAAACGCAAG GAGGTGGATG CGTCTGAGAT CCAGGTGCAG GTGTGTTTGT 
     3001 ACGCCTTCGA CCTCATCTAC CTCAATGGAG AGTCCCTGGT ACGTGAGCCC CTTTCCCGGC 
     3061 GCCGGCAGCT GCTCCGGGAG AACTTTGTGG AGACAGAGGG CGAGTTTGTC TTCGCCACCT 
     3121 CCCTGGACAC CAAGGACATC GAGCAGATCG CCGAGTTCCT GGAGCAGTCA GTGAAAGACT 
     3181 CCTGCGAGGG GCTGATGGTG AAGACCCTGG ATGTTGATGC CACCTACGAG ATCGCCAAGA 
     3241 GATCGCACAA CTGGCTCAAG CTGAAGAAGG ACTACCTTGA TGGCGTGGGT GACACCCTGG 
     3301 ACCTGGTGGT GATCGGCGCC TACCTGGGCC GGGGGAAGCG GGCCGGCCGG TACGGGGGCT 
     3361 TCCTGCTGGC CTCCTACGAC GAGGACAGTG AGGAGCTGCA GGCCATATGC AAGCTTGGAA 
     3421 CTGGCTTCAG TGATGAGGAG CTGGAGGAGC ATCACCAGAG CCTCAAGGCG CTGGTGCTGC 
     3481 CCAGCCCACG CCCTTACGTG CGGATAGATG GCGCTGTGAT TCCCGACCAC TGGCTGGACC 
     3541 CCAGCGCTGT GTGGGAGGTG AAGTGCGCTG ACCTCTCCCT CTCTCCCATC TACCCTGCTG 
     3601 CGCGGGGCCT GGTGGATAGT GACAAGGGCA TCTCCCTTCG CTTCCCTCGG TTTATTCGAG 
     3661 TCCGTGAAGA CAAGCAGCCG GAGCAGGCCA CCACCAGTGC TCAGGTGGCC TGTTTGTACC 
     3721 GGAAGCAAAG TCAGATTCAG AACCAACAAG GCGAGGACTC AGGCTCTGAC CCTGAAGATA 
     3781 CCTACACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     3841 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     3901 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     3961 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4021 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4081 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4141 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4201 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4261 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4321 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4381 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4441 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4501 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     4561 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     4621 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     4681 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     4741 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     4801 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     4861 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     4921 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     4981 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5041 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5101 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5161 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     5221 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     5281 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     5341 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     5401 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     5461 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     5521 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     5581 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     5641 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     5701 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     5761 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     5821 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     5881 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     5941 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6001 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6061 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6121 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6181 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     6241 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     6301 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     6361 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     6421 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     6481 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     6541 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     6601 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     6661 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     6721 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     6781 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     6841 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     6901 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6961 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7021 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7081 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7141 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7201 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     7261 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     7321 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     7381 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     7441 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     7501 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     7561 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     7621 AAATTTAACG CGAATTTT
//