LOCUS       RC210597L1                8763 bp    DNA     circular  SYN 
DEFINITION  VPS35 (Myc-DDK-tagged)-Human vacuolar protein sorting 35 homolog (S. cerevisiae) (VPS35)
ACCESSION   NM_018206
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7054..7234 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    7962..8621 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7393..7961 
                     /label="ColE1"  
     misc_feature    5929..5949 
                     /label="LR50 primer"  
     misc_feature    5726..5797 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    5938..6529 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5708..5714 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5708 
                     /label="VPS35"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GCCTACAACA CAGCAGTCCC CTCAGGATGA 
     3361 GCAGGAAAAG CTCTTGGATG AAGCCATACA GGCTGTGAAG GTCCAGTCAT TCCAAATGAA 
     3421 GAGATGCCTG GACAAAAACA AGCTTATGGA TGCTCTAAAA CATGCTTCTA ATATGCTTGG 
     3481 TGAACTCCGG ACTTCTATGT TATCACCAAA GAGTTACTAT GAACTTTATA TGGCCATTTC 
     3541 TGATGAACTG CACTACTTGG AGGTCTACCT GACAGATGAG TTTGCTAAAG GAAGGAAAGT 
     3601 GGCAGATCTC TACGAACTTG TACAGTATGC TGGAAACATT ATCCCAAGGC TTTACCTTTT 
     3661 GATCACAGTT GGAGTTGTAT ATGTCAAGTC ATTTCCTCAG TCCAGGAAGG ATATTTTGAA 
     3721 AGATTTGGTA GAAATGTGCC GTGGTGTGCA ACATCCCTTG AGGGGTCTGT TTCTTCGAAA 
     3781 TTACCTTCTT CAGTGTACCA GAAATATCTT ACCTGATGAA GGAGAGCCAA CAGATGAAGA 
     3841 AACAACTGGT GACATCAGTG ATTCCATGGA TTTTGTACTG CTCAACTTTG CAGAAATGAA 
     3901 CAAGCTCTGG GTGCGAATGC AGCATCAGGG ACATAGCCGA GATAGAGAAA AAAGAGAACG 
     3961 AGAAAGACAA GAACTGAGAA TTTTAGTGGG AACAAATTTG GTGCGCCTCA GTCAGTTGGA 
     4021 AGGTGTAAAT GTGGAACGTT ACAAACAGAT TGTTTTGACT GGCATATTGG AGCAAGTTGT 
     4081 AAACTGTAGG GATGCTTTGG CTCAAGAATA TCTCATGGAG TGTATTATTC AGGTTTTCCC 
     4141 TGATGAATTT CACCTCCAGA CTTTGAATCC TTTTCTTCGG GCCTGTGCTG AGTTACACCA 
     4201 GAATGTAAAT GTGAAGAACA TAATCATTGC TTTAATTGAT AGATTAGCTT TATTTGCTCA 
     4261 CCGTGAAGAT GGACCTGGAA TCCCAGCGGA TATTAAACTT TTTGATATAT TTTCACAGCA 
     4321 GGTGGCTACA GTGATACAGT CTAGACAAGA CATGCCTTCA GAGGATGTTG TATCTTTACA 
     4381 AGTCTCTCTG ATTAATCTTG CCATGAAATG TTACCCTGAT CGTGTGGACT ATGTTGATAA 
     4441 AGTTCTAGAA ACAACAGTGG AGATATTCAA TAAGCTCAAC CTTGAACATA TTGCTACCAG 
     4501 TAGTGCAGTT TCAAAGGAAC TCACCAGACT TTTGAAAATA CCAGTTGACA CTTACAACAA 
     4561 TATTTTAACA GTCTTGAAAT TAAAACATTT TCACCCACTC TTTGAGTACT TTGACTACGA 
     4621 GTCCAGAAAG AGCATGAGTT GTTATGTGCT TAGTAATGTT CTGGATTATA ACACAGAAAT 
     4681 TGTCTCTCAA GACCAGGTGG ATTCCATAAT GAATTTGGTA TCCACGTTGA TTCAAGATCA 
     4741 GCCAGATCAA CCTGTAGAAG ACCCTGATCC AGAAGATTTT GCTGATGAGC AGAGCCTTGT 
     4801 GGGCCGCTTC ATTCATCTGC TGCGCTCTGA GGACCCTGAC CAGCAGTACT TGATTTTGAA 
     4861 CACAGCACGA AAACATTTTG GAGCTGGTGG AAATCAGCGG ATTCGCTTCA CACTGCCACC 
     4921 TTTGGTATTT GCAGCTTACC AGCTGGCTTT TCGATATAAA GAGAATTCTA AAGTGGATGA 
     4981 CAAATGGGAA AAGAAATGCC AGAAGATTTT TTCATTTGCC CACCAGACTA TCAGTGCTTT 
     5041 GATCAAAGCA GAGCTGGCAG AATTGCCCTT AAGACTTTTT CTTCAAGGAG CACTAGCTGC 
     5101 TGGGGAAATT GGTTTTGAAA ATCATGAGAC AGTCGCATAT GAATTCATGT CCCAGGCATT 
     5161 TTCTCTGTAT GAAGATGAAA TCAGCGATTC CAAAGCACAG CTAGCTGCCA TCACCTTGAT 
     5221 CATTGGCACT TTTGAAAGGA TGAAGTGCTT CAGTGAAGAG AATCATGAAC CTCTGAGGAC 
     5281 TCAGTGTGCC CTTGCTGCAT CCAAACTTCT AAAGAAACCT GATCAGGGCC GAGCTGTGAG 
     5341 CACCTGTGCA CATCTCTTCT GGTCTGGCAG AAACACGGAC AAAAATGGGG AGGAGCTTCA 
     5401 CGGAGGCAAG AGGGTAATGG AGTGCCTAAA AAAAGCTCTA AAAATAGCAA ATCAGTGCAT 
     5461 GGACCCCTCT CTACAAGTGC AGCTTTTTAT AGAAATTCTG AACAGATATA TCTATTTTTA 
     5521 TGAAAAGGAA AATGATGCGG TAACAATTCA GGTTTTAAAC CAGCTTATCC AAAAGATTCG 
     5581 AGAAGACCTC CCGAATCTTG AATCCAGTGA AGAAACAGAG CAGATTAACA AACATTTTCA 
     5641 TAACACACTG GAGCATTTGC GCTTGCGGCG GGAATCACCA GAATCCGAGG GGCCAATTTA 
     5701 TGAAGGTCTC ATCCTTACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA 
     5761 TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG 
     5821 CCGCGGTCTG TACAAGTAGG ATTCGTCGAG GGACCTAATA ACTTCGTATA GCATACATTA 
     5881 TACGAAGTTA TACATGTTTA AGGGTTCCGG TTCCACTAGG TACAATTCGA TATCAAGCTT 
     5941 ATCGATAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT 
     6001 GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT 
     6061 TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG 
     6121 GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC 
     6181 CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC 
     6241 CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT 
     6301 CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA AATCATCGTC CTTTCCTTGG 
     6361 CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG 
     6421 GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG 
     6481 CGTCTTCGCC TTCGCCCTCA GACGAGTCGG ATCTCCCTTT GGGCCGCCTC CCCGCATCGA 
     6541 TACCGTCGAC CTCGATCGAG ACCTAGAAAA ACATGGAGCA ATCACAAGTA GCAATACAGC 
     6601 AGCTACCAAT GCTGATTGTG CCTGGCTAGA AGCACAAGAG GAGGAGGAGG TGGGTTTTCC 
     6661 AGTCACACCT CAGGTACCTT TAAGACCAAT GACTTACAAG GCAGCTGTAG ATCTTAGCCA 
     6721 CTTTTTAAAA GAAAAGGGGG GACTGGAAGG GCTAATTCAC TCCCAACGAA GACAAGATAT 
     6781 CCTTGATCTG TGGATCTACC ACACACAAGG CTACTTCCCT GATTGGCAGA ACTACACACC 
     6841 AGGGCCAGGG ATCAGATATC CACTGACCTT TGGATGGTGC TACAAGCTAG TACCAGTTGA 
     6901 GCAAGAGAAG GTAGAAGAAG CCAATGAAGG AGAGAACACC CGCTTGTTAC ACCCTGTGAG 
     6961 CCTGCATGGG ATGGATGACC CGGAGAGAGA AGTATTAGAG TGGAGGTTTG ACAGCCGCCT 
     7021 AGCATTTCAT CACATGGCCC GAGAGCTGCA TCCGGACTGT ACTGGGTCTC TCTGGTTAGA 
     7081 CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA 
     7141 AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA 
     7201 GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGCATGT GAGCAAAAGG 
     7261 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     7321 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     7381 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     7441 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     7501 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     7561 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     7621 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     7681 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     7741 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     7801 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     7861 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     7921 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA TTACGCCCCG 
     7981 CCCTGCCACT CATCGCAGTA CTGTTGTAAT TCATTAAGCA TTCTGCCGAC ATGGAAGCCA 
     8041 TCACAAACGG CATGATGAAC CTGAATCGCC AGCGGCATCA GCACCTTGTC GCCTTGCGTA 
     8101 TAATATTTGC CCATGGTGAA AACGGGGGCG AAGAAGTTGT CCATATTGGC CACGTTTAAA 
     8161 TCAAAACTGG TGAAACTCAC CCAGGGATTG GCTGAGACGA AAAACATATT CTCAATAAAC 
     8221 CCTTTAGGGA AATAGGCCAG GTTTTCACCG TAACACGCCA CATCTTGCGA ATATATGTGT 
     8281 AGAAACTGCC GGAAATCGTC GTGGTATTCA CTCCAGAGCG ATGAAAACGT TTCAGTTTGC 
     8341 TCATGGAAAA CGGTGTAACA AGGGTGAACA CTATCCCATA TCACCAGCTC ACCGTCTTTC 
     8401 ATTGCCATAC GGAACTCCGG ATGAGCATTC ATCAGGCGGG CAAGAATGTG AATAAAGGCC 
     8461 GGATAAAACT TGTGCTTATT TTTCTTTACG GTCTTTAAAA AGGCCGTAAT ATCCAGCTGA 
     8521 ACGGTCTGGT TATAGGTACA TTGAGCAACT GACTGAAATG CCTCAAAATG TTCTTTACGA 
     8581 TGCCATTGGG ATATATCAAC GGTGGTATAT CCAGTGATTT TTTTCTCCAT ACTCTTCCTT 
     8641 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     8701 TGTATTTAGA AAAATAAACA AATAGGGGTC CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     8761 GAC
//