LOCUS       RC210512                8139 bp    DNA     circular  SYN 
DEFINITION  SLC12A7 (Myc-DDK-tagged)-Human solute carrier family 12 (potassium/chloride transporters), member 7 (SLC12A7)
ACCESSION   NM_006598
VERSION     NM_006598.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4296..4387 
                     /label="Myc-DDK"  
     misc_feature    4417..5002 
                     /label="PolyA signal"  
     misc_feature    4474..4493 
                     /label="Restriction Enzyme"  
     misc_feature    5278..5950 
                     /label="ColE1"  
     misc_feature    6380..7174 
                     /label="Kan/Neo"  
     misc_feature    7209..7511 
                     /label="SV40 ori"  
     misc_feature    7597..8074 
                     /label="f1 ori"  
     misc_feature    1029..1037 
                     /label="Restriction Enzyme"  
     misc_feature    4278..4284 
                     /label="Restriction Enzyme"  
     misc_feature    1029..4278 
                     /label="SLC12A7"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCGG CGCGCCCATG CCCACCAACT TCACCGTGGT GCCCGTGGAG GCTCACGCCG 
     1081 ACGGCGGCGG GGACGAGACT GCCGAGCGGA CGGAGGCTCC GGGCACCCCC GAGGGCCCCG 
     1141 AGCCCGAGCG CCCCAGCCCG GGAGATGGAA ATCCAAGAGA AAACAGCCCA TTCCTCAACA 
     1201 ATGTCGAGGT GGAACAAGAG AGCTTCTTTG AAGGGAAGAA CATGGCACTT TTCGAGGAGG 
     1261 AGATGGACAG TAACCCCATG GTGTCCTCGC TGCTCAACAA GCTGGCCAAC TACACCAACC 
     1321 TGAGCCAGGG CGTGGTGGAG CACGAGGAGG ACGAGGAGAG CCGGCGGCGG GAGGCCAAGG 
     1381 CTCCGCGCAT GGGCACCTTC ATCGGCGTCT ACCTGCCGTG CCTGCAGAAC ATCCTGGGCG 
     1441 TCATCCTCTT CCTGCGCCTG ACGTGGATCG TGGGGGTGGC TGGTGTCCTG GAGTCCTTCC 
     1501 TCATCGTGGC CATGTGCTGC ACATGTACAA TGCTGACCGC CATTTCCATG AGTGCGATCG 
     1561 CTACCAACGG TGTGGTCCCA GCTGGCGGGT CCTACTACAT GATATCGCGC TCGCTGGGAC 
     1621 CCGAGTTTGG AGGCGCTGTC GGCCTCTGCT TCTACCTGGG CACGACGTTT GCAGGGGCCA 
     1681 TGTATATTTT GGGGACCATC GAGATTTTTC TGACGTACAT CTCCCCGGGT GCGGCCATCT 
     1741 TCCAGGCGGA GGCTGCAGGT GGCGAGGCGG CCGCCATGCT GCACAACATG CGTGTGTACG 
     1801 GCACGTGCAC GCTCGTGCTC ATGGCCCTGG TGGTCTTCGT GGGCGTCAAG TATGTCAACA 
     1861 AGCTGGCGCT GGTCTTCCTG GCCTGCGTCG TGCTGTCCAT CCTGGCCATC TATGCCGGCG 
     1921 TCATCAAGTC TGCCTTCGAC CCCCCGGACA TCCCGGTCTG CCTCCTGGGG AACCGCACGC 
     1981 TGTCACGGCG CAGCTTCGAT GCCTGCGTCA AGGCCTACGG CATCCACAAC AACTCAGCCA 
     2041 CCTCCGCGCT CTGGGGCCTC TTCTGCAACG GCTCCCAGCC CAGCGCCGCC TGTGACGAGT 
     2101 ACTTCATCCA GAACAACGTC ACCGAAATCC AGGGCATCCC GGGCGCGGCC AGTGGTGTCT 
     2161 TCCTGGAGAA CCTGTGGAGT ACGTACGCGC ACGCGGGGGC GTTTGTGGAG AAGAAAGGTG 
     2221 TGCCCTCGGT GCCCGTGGCA GAGGAGAGCC GTGCCAGCGC ACTGCCCTAC GTGCTCACCG 
     2281 ACATCGCGGC CTCCTTCACC CTGCTGGTTG GCATCTACTT CCCCTCCGTG ACCGGTATCA 
     2341 TGGCGGGTTC AAACCGGTCC GGGGACCTCA AGGATGCACA GAAGTCCATC CCCACGGGGA 
     2401 CCATCCTGGC CATAGTGACG ACGTCTTTCA TCTATCTCTC CTGCATTGTG CTGTTTGGGG 
     2461 CCTGCATTGA AGGCGTGGTC TTACGAGATA AGTTCGGGGA GGCCCTGCAG GGGAACCTGG 
     2521 TCATCGGCAT GCTGGCCTGG CCCTCCCCCT GGGTCATCGT CATCGGCTCC TTCTTCTCCA 
     2581 CCTGCGGTGC CGGCCTGCAG AGCCTCACGG GGGCACCGCG CCTACTGCAG GCCATTGCCC 
     2641 GTGACGGCAT CGTCCCCTTC CTGCAGGTGT TTGGCCACGG GAAGGCCAAC GGGGAGCCCA 
     2701 CGTGGGCGCT GCTGCTGACA GTCCTCATCT GCGAGACTGG CATCCTCATC GCCTCTCTGG 
     2761 ACAGCGTGGC CCCGATCCTC TCCATGTTCT TCCTCATGTG CTACCTGTTC GTGAACCTGG 
     2821 CCTGCGCCGT GCAGACCCTG CTACGTACCC CCAACTGGCG TCCACGCTTC AAGTTCTACC 
     2881 ACTGGACCCT GTCCTTTCTG GGTATGAGCC TGTGCCTGGC GCTGATGTTC ATCTGCTCCT 
     2941 GGTACTACGC GCTGTCCGCC ATGCTCATCG CTGGCTGCAT CTACAAGTAC ATCGAGTACC 
     3001 GCGGGGCCGA GAAGGAGTGG GGCGATGGCA TCCGTGGCCT ATCCCTGAAC GCCGCCCGCT 
     3061 ACGCCCTGCT GCGCGTGGAG CACGGTCCCC CCCACACCAA GAACTGGAGG CCCCAGGTGC 
     3121 TGGTGATGCT GAACCTGGAC GCGGAGCAGG CCGTGAAGCA CCCCCGCCTG CTGTCCTTCA 
     3181 CGTCGCAGCT GAAGGCCGGC AAGGGCCTGA CCATCGTGGG CTCGGTGCTG GAGGGGACGT 
     3241 ACCTGGACAA GCACATGGAG GCTCAGCGGG CCGAGGAGAA CATACGGTCC CTAATGAGCA 
     3301 CAGAGAAGAC CAAGGGCTTC TGCCAGCTGG TGGTCTCGTC CAGCCTGCGG GATGGCATGT 
     3361 CCCACCTGAT CCAGTCGGCC GGCCTGGGCG GCCTGAAGCA CAACACGGTG CTCATGGCCT 
     3421 GGCCCGCATC CTGGAAGCAG GAGGACAACC CCTTCTCCTG GAAGAACTTT GTGGACACCG 
     3481 TCCGCGACAC CACCGCCGCG CACCAGGCTC TGCTGGTGGC CAAGAACGTC GACTCGTTTC 
     3541 CGCAAAACCA GGAGCGCTTC GGCGGGGGCC ACATCGACGT GTGGTGGATC GTGCACGACG 
     3601 GCGGCATGCT CATGCTGCTG CCCTTCCTGC TGCGCCAGCA CAAGGTGTGG AGGAAGTGCC 
     3661 GGATGCGTAT CTTCACCGTG GCCCAGGTGG ACGACAACAG CATCCAGATG AAGAAGGACC 
     3721 TGCAGATGTT CTTGTATCAC TTGCGCATCA GCGCCGAGGT GGAGGTGGTG GAGATGGTTG 
     3781 AAAACGACAT ATCTGCTTTC ACCTACGAGA GGACACTAAT GATGGAGCAG AGGTCGCAGA 
     3841 TGCTGAAGCA GATGCAGCTG TCCAAGAACG AGCAGGAGCG AGAGGCCCAG CTGATCCACG 
     3901 ACAGGAACAC CGCGTCCCAC ACCGCGGCGG CAGCCAGGAC CCAAGCGCCG CCTACGCCAG 
     3961 ACAAGGTGCA GATGACCTGG ACCAGGGAGA AGCTGATCGC TGAGAAGTAC AGGAGCAGAG 
     4021 ACACCAGCCT ATCCGGTTTC AAAGACCTCT TCAGCATGAA GCCGGACCAG TCCAACGTCA 
     4081 GGCGGATGCA CACGGCTGTG AAGCTCAATG GCGTCGTCCT CAACAAGTCC CAGGATGCGC 
     4141 AGCTGGTCCT GCTCAACATG CCAGGTCCTC CCAAAAACCG GCAGGGAGAC GAGAACTACA 
     4201 TGGAGTTTCT TGAAGTCCTG ACCGAGGGGC TGAACAGAGT CCTCCTGGTC AGGGGTGGCG 
     4261 GCCGGGAGGT GATCACCATC TACTCCACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4321 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4381 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4441 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4501 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4561 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4621 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4681 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4741 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4801 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4861 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4921 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     4981 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5041 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5101 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5161 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5221 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5281 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5341 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5401 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5461 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5521 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5581 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5641 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5701 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5761 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     5821 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     5881 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     5941 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6001 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6061 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6121 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6181 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6241 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6301 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6361 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6421 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6481 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6541 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6601 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6661 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6721 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6781 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     6841 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     6901 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     6961 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7021 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7081 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7141 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7201 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7261 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7321 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7381 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7441 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7501 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7561 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7621 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7681 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7741 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7801 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     7861 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     7921 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     7981 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8041 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8101 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//