LOCUS       RC210502                7812 bp    DNA     circular  SYN 
DEFINITION  GTF2I (Myc-DDK-tagged)-Human general transcription factor IIi (GTF2I), transcript variant 3
ACCESSION   NM_033001
VERSION     NM_033001.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3969..4060 
                     /label="Myc-DDK"  
     misc_feature    4090..4675 
                     /label="PolyA signal"  
     misc_feature    4147..4166 
                     /label="Restriction Enzyme"  
     misc_feature    4951..5623 
                     /label="ColE1"  
     misc_feature    6053..6847 
                     /label="Kan/Neo"  
     misc_feature    6882..7184 
                     /label="SV40 ori"  
     misc_feature    7270..7747 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3951..3957 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3951 
                     /label="GTF2I"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCAAGTT GCAATGTCCA CCCTCCCCGT TGAAGATGAG GAGTCCTCGG 
     1081 AGAGCAGGAT GGTGGTGACA TTCCTCATGT CAGCTCTCGA GTCCATGTGT AAAGAACTGG 
     1141 CCAAGTCCAA AGCCGAAGTG GCCTGCATTG CAGTGTATGA AACAGACGTG TTTGTCGTCG 
     1201 GAACTGAAAG AGGACGTGCT TTTGTCAATA CCAGAAAGGA TTTTCAAAAA GATTTTGTAA 
     1261 AATATTGTGT TGAAGAAGAA GAAAAAGCTG CAGAGATGCA TAAAATGAAA TCTACAACCC 
     1321 AGGCAAATCG GATGAGTGTA GATGCTGTAG AAATTGAAAC ACTCAGAAAA ACAGTTGAGG 
     1381 ACTATTTCTG CTTTTGCTAT GGGAAAGCTT TAGGCAAATC CACAGTGGTA CCTGTACCAT 
     1441 ATGAGAAGAT GCTGCGAGAC CAGTCGGCTG TGGTAGTGCA GGGGCTTCCG GAAGGTGTTG 
     1501 CCTTTAAACA CCCCGAGAAC TATGATCTTG CAACCCTGAA ATGGATTTTG GAGAACAAAG 
     1561 CAGGGATTTC ATTCATCATT AAGAGACCTT TTTTAGAGCC AAAGAAGCAT GTAGGTGGTC 
     1621 GTGTGATGGT AACAGATGCT GACAGGTCAA TACTATCTCC AGGTGGAAGT TGTGGCCCCA 
     1681 TCAAAGTGAA AACTGAACCC ACAGAAGATT CTGGCATTTC CCTGGAAATG GCAGCTGTGA 
     1741 CAGTAAAGGA AGAATCAGAA GATCCTGATT ATTATCAATA TAACATTCAA GCAGGCCCTT 
     1801 CTGAAACTGA TGATGTTGAT GAAAAACAGC CCCTATCGAA GCCTTTGCAA GGAAGCCACC 
     1861 ATTCTTCAGA GGGCAATGAA GGCACAGAAA TGGAAGTACC AGCAGAAGAT GATGATTATT 
     1921 CTCCACCGTC TAAGAGACCA AAGGCCAATG AGCTACCGCA GCCACCAGTC CCGGAACCCG 
     1981 CCAATGCTGG GAAGCGGAAA GTGAGGGAGT TCAACTTCGA GAAATGGAAT GCTCGCATCA 
     2041 CTGATCTACG TAAACAAGTT GAAGAATTGT TTGAAAGGAA ATATGCTCAA GCCATAAAAG 
     2101 CCAAAGGTCC GGTGACGATC CCGTACCCTC TTTTCCAGTC TCATGTTGAA GATCTTTATG 
     2161 TAGAAGGACT TCCTGAAGGA ATTCCTTTTA GAAGGCCATC TACTTACGGA ATTCCTCGCC 
     2221 TGGAGAGGAT ATTACTTGCA AAGGAAAGGA TTCGTTTTGT GATTAAGAAA CATGAGCTTC 
     2281 TGAATTCAAC ACGTGAAGAT TTACAGCTTG ATAAGCCAGC TTCAGGAGTA AAGGAAGAAT 
     2341 GGTATGCCAG AATCACTAAA TTAAGAAAGA TGGTGGATCA GCTTTTCTGC AAAAAATTTG 
     2401 CGGAAGCCTT GGGGAGCACT GAAGCCAAGG CTGTACCGTA CCAAAAATTT GAGGCACACC 
     2461 CGAATGATCT GTACGTGGAA GGACTGCCAG AAAACATTCC TTTCCGAAGT CCCTCATGGT 
     2521 ATGGAATCCC AAGGCTGGAA AAAATCATTC AAGTGGGCAA TCGAATTAAA TTTGTTATTA 
     2581 AAAGACCAGA ACTTCTGACT CACAGTACCA CTGAAGTTAC TCAGCCAAGA ACGAATACAC 
     2641 CAGTCAAAGA AGATTGGAAT GTCAGAATTA CCAAGCTACG GAAGCAAGTG GAAGAGATTT 
     2701 TTAATTTGAA ATTTGCTCAA GCTCTTGGAC TCACCGAGGC AGTAAAAGTA CCATATCCTG 
     2761 TGTTTGAATC AAACCCGGAG TTCTTGTATG TGGAAGGCTT GCCAGAGGGG ATTCCCTTCC 
     2821 GAAGCCCTAC CTGGTTTGGA ATTCCACGAC TTGAAAGGAT CGTCCGCGGG AGTAATAAAA 
     2881 TCAAGTTCGT TGTTAAAAAA CCTGAACTAG TTATTTCCTA CTTGCCTCCT GGGATGGCTA 
     2941 GTAAAATAAA CACTAAAGCT TTGCAGTCCC CCAAAAGACC ACGAAGTCCT GGGAGTAATT 
     3001 CAAAGGTTCC TGAAATTGAG GTCACCGTGG AAGGCCCTAA TAACAACAAT CCTCAAACCT 
     3061 CAGCTGTTCG AACCCCGACC CAGACTAACG GTTCTAACGT TCCCTTCAAG CCACGAGGGA 
     3121 GAGAGTTTTC CTTTGAGGCC TGGAATGCCA AAATCACGGA CCTAAAACAG AAAGTTGAAA 
     3181 ATCTCTTCAA TGAGAAATGT GGGGAAGCTC TTGGCCTTAA ACAAGCTGTG AAGGTGCCGT 
     3241 TCGCGTTATT TGAGTCTTTC CCGGAAGACT TTTATGTGGA AGGCTTACCT GAGGGTGTGC 
     3301 CATTCCGAAG ACCATCGACT TTTGGCATTC CGAGGCTGGA GAAGATACTC AGAAACAAAG 
     3361 CCAAAATTAA GTTCATCATT AAAAAGCCCG AAATGTTTGA GACGGCGATT AAGGAGAGCA 
     3421 CCTCCTCTAA GAGCCCTCCC AGAAAAATAA ATTCATCACC CAATGTTAAT ACTACTGCAT 
     3481 CAGGTGTTGA AGACCTTAAC ATCATTCAGG TGACAATTCC AGATGATGAT AATGAAAGAC 
     3541 TCTCGAAAGT TGAAAAAGCT AGACAGCTAA GAGAACAAGT GAATGACCTC TTTAGTCGGA 
     3601 AATTTGGTGA AGCTATTGGT ATGGGTTTTC CTGTGAAAGT TCCCTACAGG AAAATCACAA 
     3661 TTAACCCTGG CTGTGTGGTG GTTGATGGCA TGCCCCCGGG GGTGTCCTTC AAAGCCCCCA 
     3721 GCTACCTGGA AATCAGCTCC ATGAGAAGGA TCTTAGACTC TGCCGAGTTT ATCAAATTCA 
     3781 CGGTCATTAG ACCATTTCCA GGACTTGTGA TTAATAACCA GCTGGTTGAT CAGAGTGAGT 
     3841 CAGAAGGCCC CGTGATACAA GAATCAGCTG AACCAAGCCA GTTGGAAGTT CCAGCCACAG 
     3901 AAGAAATAAA AGAGACTGAT GGAAGCTCTC AGATCAAGCA AGAACCAGAC CCCACGTGGA 
     3961 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4021 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4081 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4141 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4201 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4261 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4321 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4381 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4441 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4501 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     4561 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     4621 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     4681 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     4741 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     4801 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     4861 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     4921 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     4981 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5041 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5101 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5161 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5221 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     5281 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     5341 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     5401 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     5461 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     5521 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     5581 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     5641 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     5701 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     5761 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     5821 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     5881 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     5941 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6001 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6061 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6121 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6181 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6241 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     6301 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     6361 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     6421 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     6481 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     6541 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     6601 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     6661 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     6721 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     6781 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     6841 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     6901 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     6961 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7021 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7081 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7141 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7201 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7261 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     7321 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     7381 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     7441 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     7501 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     7561 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     7621 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     7681 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     7741 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     7801 AACGCGAATT TT
//