LOCUS       RC209919                7779 bp    DNA     circular  SYN 
DEFINITION  OPA1 (Myc-DDK-tagged)-Human optic atrophy 1 (autosomal dominant) (OPA1), nuclear gene encoding mitochondrial protein, transcript variant 1
ACCESSION   NM_015560
VERSION     NM_015560.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3934..4025 
                     /label="Myc-DDK"  
     misc_feature    4055..4640 
                     /label="PolyA signal"  
     misc_feature    4112..4131 
                     /label="Restriction Enzyme"  
     misc_feature    4916..5588 
                     /label="ColE1"  
     misc_feature    6018..6812 
                     /label="Kan/Neo"  
     misc_feature    6847..7149 
                     /label="SV40 ori"  
     misc_feature    7235..7712 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3909..3916 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3909 
                     /label="OPA1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGGCGACTA CGTCGGGCCG CTGTGGCCTG TGAGGTCTGC CAGTCTTTAG 
     1081 TGAAACACAG CTCTGGAATA AAAGGAAGTT TACCACTACA AAAACTACAT CTGGTTTCAC 
     1141 GAAGCATTTA TCATTCACAT CATCCTACCT TAAAGCTTCA ACGACCCCAA TTAAGGACAT 
     1201 CCTTTCAGCA GTTCTCTTCT CTGACAAACC TTCCTTTACG TAAACTGAAA TTCTCTCCAA 
     1261 TTAAATATGG CTACCAGCCT CGCAGGAATT TTTGGCCAGC AAGATTAGCT ACGAGACTCT 
     1321 TAAAACTTCG CTATCTCATA CTAGGATCGG CTGTTGGGGG TGGCTACACA GCCAAAAAGA 
     1381 CTTTTGATCA GTGGAAAGAT ATGATACCGG ACCTTAGTGA ATATAAATGG ATTGTGCCTG 
     1441 ACATTGTGTG GGAAATTGAT GAGTATATCG ATTTTGAGAA AATTAGAAAA GCCCTTCCTA 
     1501 GTTCAGAAGA CCTTGTAAAG TTAGCACCAG ACTTTGACAA GATTGTTGAA AGCCTTAGCT 
     1561 TATTGAAGGA CTTTTTTACC TCAGGTTCTC CGGAAGAAAC GGCGTTTAGA GCAACAGATC 
     1621 GTGGATCTGA AAGTGACAAG CATTTTAGAA AGGTGTCAGA CAAAGAGAAA ATTGACCAAC 
     1681 TTCAGGAAGA ACTTCTGCAC ACTCAGTTGA AGTATCAGAG AATCTTGGAA CGATTAGAAA 
     1741 AGGAGAACAA AGAATTGAGA AAATTAGTAT TGCAGAAAGA TGACAAAGGC ATTCATCATA 
     1801 GAAAGCTTAA GAAATCTTTG ATTGACATGT ATTCTGAAGT TCTTGATGTT CTCTCTGATT 
     1861 ATGATGCCAG TTATAATACG CAAGATCATC TGCCACGGGT TGTTGTGGTT GGAGATCAGA 
     1921 GTGCTGGAAA GACTAGTGTG TTGGAAATGA TTGCCCAAGC TCGAATATTC CCAAGAGGAT 
     1981 CTGGGGAGAT GATGACACGT TCTCCAGTTA AGGTGACTCT GAGTGAAGGT CCTCACCATG 
     2041 TGGCCCTATT TAAAGATAGT TCTCGGGAGT TTGATCTTAC CAAAGAAGAA GATCTTGCAG 
     2101 CATTAAGACA TGAAATAGAA CTTCGAATGA GGAAAAATGT GAAAGAAGGC TGTACCGTTA 
     2161 GCCCTGAGAC CATATCCTTA AATGTAAAAG GCCCTGGACT ACAGAGGATG GTGCTTGTTG 
     2221 ACTTACCAGG TGTGATTAAT ACTGTGACAT CAGGCATGGC TCCTGACACA AAGGAAACTA 
     2281 TTTTCAGTAT CAGCAAAGCT TACATGCAGA ATCCTAATGC CATCATACTG TGTATTCAAG 
     2341 ATGGATCTGT GGATGCTGAA CGCAGTATTG TTACAGACTT GGTCAGTCAA ATGGACCCTC 
     2401 ATGGAAGGAG AACCATATTC GTTTTGACCA AAGTAGACCT GGCAGAGAAA AATGTAGCCA 
     2461 GTCCAAGCAG GATTCAGCAG ATAATTGAAG GAAAGCTCTT CCCAATGAAA GCTTTAGGTT 
     2521 ATTTTGCTGT TGTAACAGGA AAAGGGAACA GCTCTGAAAG CATTGAAGCT ATAAGAGAAT 
     2581 ATGAAGAAGA GTTTTTTCAG AATTCAAAGC TCCTAAAGAC AAGCATGCTA AAGGCACACC 
     2641 AAGTGACTAC AAGAAATTTA AGCCTTGCAG TATCAGACTG CTTTTGGAAA ATGGTACGAG 
     2701 AGTCTGTTGA ACAACAGGCT GATAGTTTCA AAGCAACACG TTTTAACCTT GAAACTGAAT 
     2761 GGAAGAATAA CTATCCTCGC CTGCGGGAAC TTGACCGGAA TGAACTATTT GAAAAAGCTA 
     2821 AAAATGAAAT CCTTGATGAA GTTATCAGTC TGAGCCAGGT TACACCAAAA CATTGGGAGG 
     2881 AAATCCTTCA ACAATCTTTG TGGGAAAGAG TATCAACTCA TGTGATTGAA AACATCTACC 
     2941 TTCCAGCTGC GCAGACCATG AATTCAGGAA CTTTTAACAC CACAGTGGAT ATCAAGCTTA 
     3001 AACAGTGGAC TGATAAACAA CTTCCTAATA AAGCAGTAGA GGTTGCTTGG GAGACCCTAC 
     3061 AAGAAGAATT TTCCCGCTTT ATGACAGAAC CGAAAGGGAA AGAGCATGAT GACATATTTG 
     3121 ATAAACTTAA AGAGGCTGTT AAGGAAGAAA GTATTAAACG ACACAAGTGG AATGACTTTG 
     3181 CGGAGGACAG CTTGAGGGTT ATTCAACACA ATGCTTTGGA AGACCGATCC ATATCTGATA 
     3241 AACAGCAATG GGATGCAGCT ATTTATTTTA TGGAAGAGGC TCTGCAGGCT CGTCTCAAGG 
     3301 ATACTGAAAA TGCAATTGAA AACATGGTGG GTCCAGACTG GAAAAAGAGG TGGTTATACT 
     3361 GGAAGAATCG GACCCAAGAA CAGTGTGTTC ACAATGAAAC CAAGAATGAA TTGGAGAAGA 
     3421 TGTTGAAATG TAATGAGGAG CACCCAGCTT ATCTTGCAAG TGATGAAATA ACCACAGTCC 
     3481 GGAAGAACCT TGAATCCCGA GGAGTAGAAG TAGATCCAAG CTTGATTAAG GATACTTGGC 
     3541 ATCAAGTTTA TAGAAGACAT TTTTTAAAAA CAGCTCTAAA CCATTGTAAC CTTTGTCGAA 
     3601 GAGGTTTTTA TTACTACCAA AGGCATTTTG TAGATTCTGA GTTGGAATGC AATGATGTGG 
     3661 TCTTGTTTTG GCGTATACAG CGCATGCTTG CTATCACCGC AAATACTTTA AGGCAACAAC 
     3721 TTACAAATAC TGAAGTTAGG CGATTAGAGA AAAATGTTAA AGAGGTATTG GAAGATTTTG 
     3781 CTGAAGATGG TGAGAAGAAG ATTAAATTGC TTACTGGTAA ACGCGTTCAA CTGGCGGAAG 
     3841 ACCTCAAGAA AGTTAGAGAA ATTCAAGAAA AACTTGATGC TTTCATTGAA GCTCTTCATC 
     3901 AGGAGAAAAG CGGACCGAGC GGACCGACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     3961 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4021 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4081 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4141 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4201 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4261 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4321 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4381 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4441 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4501 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4561 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     4621 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     4681 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     4741 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     4801 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     4861 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     4921 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     4981 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5041 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5101 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5161 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5221 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5281 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5341 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5401 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     5461 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     5521 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     5581 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     5641 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     5701 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     5761 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     5821 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     5881 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     5941 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6001 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6061 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6121 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6181 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6241 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6301 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6361 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6421 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     6481 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     6541 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     6601 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     6661 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     6721 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     6781 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     6841 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     6901 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     6961 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7021 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7081 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7141 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7201 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7261 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7321 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7381 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7441 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     7501 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     7561 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     7621 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     7681 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     7741 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//