LOCUS       RC209907                6037 bp    DNA     circular  SYN 
DEFINITION  LPAR5 (Myc-DDK-tagged)-Human lysophosphatidic acid receptor 5 (LPAR5), transcript variant 1
ACCESSION   NM_020400
VERSION     NM_020400.5
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2194..2285 
                     /label="Myc-DDK"  
     misc_feature    2315..2900 
                     /label="PolyA signal"  
     misc_feature    2372..2391 
                     /label="Restriction Enzyme"  
     misc_feature    3176..3848 
                     /label="ColE1"  
     misc_feature    4278..5072 
                     /label="Kan/Neo"  
     misc_feature    5107..5409 
                     /label="SV40 ori"  
     misc_feature    5495..5972 
                     /label="f1 ori"  
     misc_feature    1060..1067 
                     /label="Restriction Enzyme"  
     misc_feature    2176..2182 
                     /label="Restriction Enzyme"  
     misc_feature    1060..2176 
                     /label="LPAR5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCGG CGCGCCAGAT CTCAAGCTTA ACTAGCTAGC GGACCGACAT GTTAGCCAAC 
     1081 AGCTCCTCAA CCAACAGTTC TGTTCTCCCG TGTCCTGACT ACCGACCTAC CCACCGCCTG 
     1141 CACTTGGTGG TCTACAGCTT GGTGCTGGCT GCCGGGCTCC CCCTCAACGC GCTAGCCCTC 
     1201 TGGGTCTTCC TGCGCGCGCT GCGCGTGCAC TCGGTGGTGA GCGTGTACAT GTGTAACCTG 
     1261 GCGGCCAGCG ACCTGCTCTT CACCCTCTCG CTGCCCGTTC GTCTCTCCTA CTACGCACTG 
     1321 CACCACTGGC CCTTCCCCGA CCTCCTGTGC CAGACGACGG GCGCCATCTT CCAGATGAAC 
     1381 ATGTACGGCA GCTGCATCTT CCTGATGCTC ATCAACGTGG ACCGCTACGC CGCCATCGTG 
     1441 CACCCGCTGC GACTGCGCCA CCTGCGGCGG CCCCGCGTGG CGCGGCTGCT CTGCCTGGGC 
     1501 GTGTGGGCGC TCATCCTGGT GTTTGCCGTG CCCGCCGCCC GCGTGCACAG GCCCTCGCGT 
     1561 TGCCGCTACC GGGACCTCGA GGTGCGCCTA TGCTTCGAGA GCTTCAGCGA CGAGCTGTGG 
     1621 AAAGGCAGGC TGCTGCCCCT CGTGCTGCTG GCCGAGGCGC TGGGCTTCCT GCTGCCCCTG 
     1681 GCGGCGGTGG TCTACTCGTC GGGCCGAGTC TTCTGGACGC TGGCGCGCCC CGACGCCACG 
     1741 CAGAGCCAGC GGCGGCGGAA GACCGTGCGC CTCCTGCTGG CTAACCTCGT CATCTTCCTG 
     1801 CTGTGCTTCG TGCCCTACAA CAGCACGCTG GCGGTCTACG GGCTGCTGCG GAGCAAGCTG 
     1861 GTGGCGGCCA GCGTGCCTGC CCGCGATCGC GTGCGCGGGG TGCTGATGGT GATGGTGCTG 
     1921 CTGGCCGGCG CCAACTGCGT GCTGGACCCG CTGGTGTACT ACTTTAGCGC CGAGGGCTTC 
     1981 CGCAACACCC TGCGCGGCCT GGGCACTCCG CACCGGGCCA GGACCTCGGC CACCAACGGG 
     2041 ACGCGGGCGG CGCTCGCGCA ATCCGAAAGG TCCGCCGTCA CCACCGACGC CACCAGGCCG 
     2101 GATGCCGCCA GTCAGGGGCT GCTCCGACCC TCCGACTCCC ACTCTCTGTC TTCCTTCACA 
     2161 CAGTGTCCCC AGGATTCCGC CCTCACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA 
     2221 GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA 
     2281 ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC 
     2341 CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT 
     2401 AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG 
     2461 GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT 
     2521 CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC 
     2581 CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT 
     2641 GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG 
     2701 GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG 
     2761 ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA 
     2821 GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT 
     2881 GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA 
     2941 ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT 
     3001 CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA 
     3061 GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC 
     3121 ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC 
     3181 AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG 
     3241 GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA 
     3301 GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG 
     3361 TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC 
     3421 ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG 
     3481 GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT 
     3541 TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT 
     3601 CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC 
     3661 GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT 
     3721 GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT 
     3781 AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG 
     3841 AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC 
     3901 TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG 
     3961 GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC 
     4021 CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA 
     4081 TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA 
     4141 GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC 
     4201 TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT 
     4261 CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC 
     4321 GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC 
     4381 GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC 
     4441 CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT 
     4501 CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT 
     4561 GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG 
     4621 ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG 
     4681 GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT 
     4741 GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC 
     4801 CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC 
     4861 GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC 
     4921 GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC 
     4981 GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA 
     5041 AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC 
     5101 TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT 
     5161 GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC 
     5221 CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG 
     5281 GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG 
     5341 AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC 
     5401 TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT 
     5461 TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG 
     5521 TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG 
     5581 CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG 
     5641 TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT 
     5701 CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG 
     5761 GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA 
     5821 TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC 
     5881 GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC 
     5941 TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA 
     6001 AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//