LOCUS       RC209819L1                8943 bp    DNA     circular  SYN 
DEFINITION  ATP6V0A2 (Myc-DDK-tagged)-Human ATPase, H+ transporting, lysosomal V0 subunit a2 (ATP6V0A2)
ACCESSION   NM_012463
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7234..7414 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8142..8801 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7573..8141 
                     /label="ColE1"  
     misc_feature    6109..6129 
                     /label="LR50 primer"  
     misc_feature    5906..5977 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6118..6709 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5888..5894 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5888 
                     /label="ATP6V0A2"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGGGTCCCTG TTCCGGAGCG AGACCATGTG 
     3361 CCTGGCGCAG CTCTTCCTGC AGTCGGGCAC GGCCTACGAG TGCCTCAGCG CCCTGGGCGA 
     3421 GAAAGGCCTG GTCCAGTTCC GAGACCTCAA CCAGAACGTA AGTTCTTTCC AAAGAAAATT 
     3481 TGTTGGTGAG GTGAAGAGGT GTGAAGAGCT AGAGCGAATA TTGGTGTATT TGGTACAGGA 
     3541 AATTAATAGA GCTGATATTC CCCTTCCTGA AGGAGAGGCC AGCCCTCCTG CGCCACCCCT 
     3601 GAAACAGGTT CTAGAAATGC AGGAGCAGTT GCAGAAGCTC GAGGTTGAAC TGAGAGAAGT 
     3661 CACTAAGAAC AAGGAGAAAC TGAGGAAAAA CTTGCTGGAA CTGATAGAGT ACACTCACAT 
     3721 GCTGAGAGTG ACAAAGACCT TTGTGAAACG CAATGTTGAG TTTGAACCCA CTTATGAAGA 
     3781 ATTCCCTTCC TTAGAGAGTG ATTCTTTGTT GGATTACAGC TGTATGCAGA GGCTGGGAGC 
     3841 AAAACTGGGA TTTGTGTCTG GCCTAATTAA CCAAGGAAAA GTGGAAGCAT TTGAAAAAAT 
     3901 GTTGTGGAGA GTCTGCAAAG GGTACACCAT CGTGTCCTAT GCAGAACTGG ATGAATCCCT 
     3961 TGAAGACCCT GAAACAGGGG AAGTCATAAA ATGGTATGTC TTTTTAATAT CCTTTTGGGG 
     4021 AGAGCAGATT GGCCACAAGG TTAAGAAGAT ATGTGATTGC TACCACTGCC ACGTGTACCC 
     4081 CTATCCAAAC ACAGCCGAGG AGCGGAGGGA GATCCAGGAG GGGCTGAACA CCCGCATCCA 
     4141 GGATCTCTAC ACTGTACTGC ACAAAACCGA GGACTATTTG AGGCAAGTGC TATGTAAAGC 
     4201 CGCCGAGTCT GTCTACAGCC GTGTGATCCA GGTGAAGAAA ATGAAGGCCA TCTATCACAT 
     4261 GCTGAACATG TGCAGCTTTG ACGTGACCAA CAAGTGCCTC ATTGCTGAGG TCTGGTGTCC 
     4321 CGAGGCGGAT CTGCAGGACC TGCGCCGGGC ACTGGAGGAG GGCTCGAGAG AGAGTGGTGC 
     4381 TACAATCCCC TCATTCATGA ATATAATCCC CACAAAAGAA ACACCCCCCA CTCGGATCCG 
     4441 CACCAACAAA TTCACCGAGG GATTTCAGAA CATCGTGGAT GCTTATGGAG TCGGAAGCTA 
     4501 CAGAGAAGTC AATCCAGCTC TCTTTACCAT CATCACCTTC CCGTTTTTAT TTGCTGTGAT 
     4561 GTTTGGAGAC TTCGGACATG GCTTTGTGAT GTTTTTATTT GCCCTCTTGT TGGTGTTAAA 
     4621 TGAAAATCAT CCCAGACTAA ATCAGTCACA AGAGATCATG AGGATGTTTT TTAATGGCCG 
     4681 GTACATCCTC CTGCTGATGG GGCTGTTCTC AGTGTACACT GGCCTCATCT ACAACGACTG 
     4741 CTTTTCAAAG TCAGTCAACC TGTTCGGCTC TGGGTGGAAC GTGTCGGCCA TGTACAGCTC 
     4801 CAGCCACCCA CCCGCAGAGC ATAAGAAGAT GGTGCTTTGG AATGACAGCG TCGTTAGACA 
     4861 CAACAGCATT TTGCAGCTGG ATCCAAGCAT TCCTGGAGTG TTCCGAGGCC CTTATCCCCT 
     4921 TGGCATTGAT CCTATTTGGA ACTTGGCCAC AAATCGCCTC ACTTTTCTAA ACTCTTTCAA 
     4981 AATGAAAATG TCCGTGATTT TAGGAATCAT TCATATGACT TTTGGAGTCA TTCTGGGAAT 
     5041 ATTTAACCAC TTGCACTTCA GGAAGAAGTT CAACATTTAC CTGGTTTCCA TCCCGGAACT 
     5101 TCTCTTCATG CTCTGTATCT TTGGATACCT TATATTTATG ATTTTCTACA AGTGGCTGGT 
     5161 TTTTTCAGCA GAAACCTCCA GAGTTGCTCC CAGCATTCTG ATTGAATTTA TTAACATGTT 
     5221 TTTATTCCCA GCCAGTAAAA CAAGTGGCCT TTACACAGGG CAGGAGTATG TCCAGAGAGT 
     5281 GCTGCTGGTT GTCACAGCAT TGTCTGTCCC TGTCCTCTTC TTGGGAAAGC CACTGTTTTT 
     5341 GTTGTGGCTT CACAATGGGC GTAGTTGCTT CGGGGTGAAC CGGAGTGGCT ACACACTTAT 
     5401 AAGGAAAGAT AGTGAGGAAG AAGTTTCATT GCTGGGAAGC CAAGATATAG AAGAGGGAAA 
     5461 TCACCAGGTG GAAGATGGAT GTAGAGAAAT GGCGTGTGAA GAGTTTAATT TTGGAGAAAT 
     5521 ATTAATGACC CAAGTAATCC ATTCCATCGA GTACTGTCTG GGATGCATCT CCAACACCGC 
     5581 CTCCTACCTG AGGCTCTGGG CGCTTAGCCT GGCTCACGCA CAGTTGTCTG ATGTCCTGTG 
     5641 GGCCATGCTG ATGCGCGTGG GCCTCCGCGT TGACACCACC TATGGCGTCT TGCTACTGCT 
     5701 CCCGGTTATC GCGCTCTTTG CAGTTTTGAC CATTTTCATC CTTCTGATCA TGGAAGGGCT 
     5761 TTCTGCGTTT CTTCACGCCA TACGCCTCCA CTGGGTAGAA TTTCAGAACA AATTCTACGT 
     5821 TGGTGCAGGC ACCAAATTTG TTCCTTTCTC ATTCAGTCTA CTTTCATCAA AGTTCAATAA 
     5881 CGACGACAGT GTGGCAACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA 
     5941 TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG 
     6001 CCGCGGTCTG TACAAGTAGG ATTCGTCGAG GGACCTAATA ACTTCGTATA GCATACATTA 
     6061 TACGAAGTTA TACATGTTTA AGGGTTCCGG TTCCACTAGG TACAATTCGA TATCAAGCTT 
     6121 ATCGATAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT 
     6181 GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT 
     6241 TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG 
     6301 GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC 
     6361 CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC 
     6421 CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT 
     6481 CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA AATCATCGTC CTTTCCTTGG 
     6541 CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG 
     6601 GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG 
     6661 CGTCTTCGCC TTCGCCCTCA GACGAGTCGG ATCTCCCTTT GGGCCGCCTC CCCGCATCGA 
     6721 TACCGTCGAC CTCGATCGAG ACCTAGAAAA ACATGGAGCA ATCACAAGTA GCAATACAGC 
     6781 AGCTACCAAT GCTGATTGTG CCTGGCTAGA AGCACAAGAG GAGGAGGAGG TGGGTTTTCC 
     6841 AGTCACACCT CAGGTACCTT TAAGACCAAT GACTTACAAG GCAGCTGTAG ATCTTAGCCA 
     6901 CTTTTTAAAA GAAAAGGGGG GACTGGAAGG GCTAATTCAC TCCCAACGAA GACAAGATAT 
     6961 CCTTGATCTG TGGATCTACC ACACACAAGG CTACTTCCCT GATTGGCAGA ACTACACACC 
     7021 AGGGCCAGGG ATCAGATATC CACTGACCTT TGGATGGTGC TACAAGCTAG TACCAGTTGA 
     7081 GCAAGAGAAG GTAGAAGAAG CCAATGAAGG AGAGAACACC CGCTTGTTAC ACCCTGTGAG 
     7141 CCTGCATGGG ATGGATGACC CGGAGAGAGA AGTATTAGAG TGGAGGTTTG ACAGCCGCCT 
     7201 AGCATTTCAT CACATGGCCC GAGAGCTGCA TCCGGACTGT ACTGGGTCTC TCTGGTTAGA 
     7261 CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA 
     7321 AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA 
     7381 GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGCATGT GAGCAAAAGG 
     7441 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     7501 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     7561 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     7621 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     7681 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     7741 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     7801 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     7861 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     7921 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     7981 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     8041 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     8101 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA TTACGCCCCG 
     8161 CCCTGCCACT CATCGCAGTA CTGTTGTAAT TCATTAAGCA TTCTGCCGAC ATGGAAGCCA 
     8221 TCACAAACGG CATGATGAAC CTGAATCGCC AGCGGCATCA GCACCTTGTC GCCTTGCGTA 
     8281 TAATATTTGC CCATGGTGAA AACGGGGGCG AAGAAGTTGT CCATATTGGC CACGTTTAAA 
     8341 TCAAAACTGG TGAAACTCAC CCAGGGATTG GCTGAGACGA AAAACATATT CTCAATAAAC 
     8401 CCTTTAGGGA AATAGGCCAG GTTTTCACCG TAACACGCCA CATCTTGCGA ATATATGTGT 
     8461 AGAAACTGCC GGAAATCGTC GTGGTATTCA CTCCAGAGCG ATGAAAACGT TTCAGTTTGC 
     8521 TCATGGAAAA CGGTGTAACA AGGGTGAACA CTATCCCATA TCACCAGCTC ACCGTCTTTC 
     8581 ATTGCCATAC GGAACTCCGG ATGAGCATTC ATCAGGCGGG CAAGAATGTG AATAAAGGCC 
     8641 GGATAAAACT TGTGCTTATT TTTCTTTACG GTCTTTAAAA AGGCCGTAAT ATCCAGCTGA 
     8701 ACGGTCTGGT TATAGGTACA TTGAGCAACT GACTGAAATG CCTCAAAATG TTCTTTACGA 
     8761 TGCCATTGGG ATATATCAAC GGTGGTATAT CCAGTGATTT TTTTCTCCAT ACTCTTCCTT 
     8821 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     8881 TGTATTTAGA AAAATAAACA AATAGGGGTC CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     8941 GAC
//