LOCUS       RC209781                8259 bp    DNA     circular  SYN 
DEFINITION  USP36 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 36 (USP36)
ACCESSION   NM_025090
VERSION     NM_025090.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4414..4505 
                     /label="Myc-DDK"  
     misc_feature    4535..5120 
                     /label="PolyA signal"  
     misc_feature    4592..4611 
                     /label="Restriction Enzyme"  
     misc_feature    5396..6068 
                     /label="ColE1"  
     misc_feature    6498..7292 
                     /label="Kan/Neo"  
     misc_feature    7327..7629 
                     /label="SV40 ori"  
     misc_feature    7715..8192 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4389..4396 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4389 
                     /label="USP36"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCAATAGTG GATAAGTTGA AGGAGGCCCT GAAACCCGGC CGCAAGGACT 
     1081 CGGCTGATGA TGGAGAACTG GGGAAGCTTC TTGCCTCCTC TGCCAAGAAG GTCCTTTTAC 
     1141 AGAAAATCGA GTTCGAGCCA GCCAGCAAGA GCTTCTCCTA CCAGCTGGAG GCCTTAAAGA 
     1201 GCAAATATGT GTTGCTCAAC CCCAAAACAG AGGGAGCTAG TCGCCACAAG AGTGGAGATG 
     1261 ACCCACCGGC CGGGAGACAG GGCAGTGAGC ACACGTATGA GAGCTGTGGT GACGGAGTCC 
     1321 CAGCCCCGCA GAAAGTGCTT TTCCCCACGG AGCGACTGTC TCTGAGGTGG GAGCGGGTCT 
     1381 TCCGCGTGGG CGCAGGACTC CACAACCTTG GCAACACCTG CTTTCTCAAT GCCACCATCC 
     1441 AGTGCTTGAC CTACACACCA CCTCTAGCCA ACTACCTGCT CTCCAAGGAG CATGCTCGCA 
     1501 GCTGCCACCA GGGAAGCTTC TGCATGCTGT GTGTCATGCA GAACCACATT GTCCAGGCCT 
     1561 TCGCCAACAG CGGCAACGCC ATCAAGCCCG TCTCCTTCAT CCGAGACCTG AAAAAGATCG 
     1621 CCCGACACTT CCGCTTTGGG AACCAGGAGG ACGCGCATGA GTTCCTGCGG TACACCATCG 
     1681 ACGCCATGCA GAAAGCCTGC CTGAATGGCT GTGCCAAGTT GGATCGTCAA ACGCAGGCTA 
     1741 CTACCTTGGT CCATCAAATT TTTGGAGGGT ATCTCAGATC ACGCGTGAAG TGCTCCGTGT 
     1801 GCAAGAGCGT CTCGGACACC TACGACCCCT ACTTGGACGT CGCGCTGGAG ATCCGGCAAG 
     1861 CTGCGAATAT TGTGCGTGCT CTGGAACTTT TTGTGAAAGC AGATGTCCTG AGTGGAGAGA 
     1921 ATGCCTACAT GTGTGCTAAA TGCAAGAAGA AGGTTCCAGC CAGCAAGCGC TTCACCATCC 
     1981 ACAGAACATC CAACGTCTTA ACCCTTTCCC TCAAGCGCTT TGCCAACTTC AGCGGGGGGA 
     2041 AGATCACCAA GGATGTAGGC TATCCGGAAT TCCTCAACAT ACGTCCGTAT ATGTCCCAGA 
     2101 ATAATGGTGA TCCTGTCATG TATGGACTCT ATGCTGTCCT GGTGCACTCG GGCTACAGCT 
     2161 GCCATGCCGG GCACTATTAC TGCTACGTGA AGGCAAGCAA TGGACAGTGG TACCAGATGA 
     2221 ATGATTCCTT GGTCCATTCC AGCAACGTCA AGGTGGTTCT GAACCAGCAG GCCTACGTGC 
     2281 TGTTCTATCT GCGAATTCCA GGCTCTAAGA AAAGTCCCGA GGGCCTCATC TCCAGGACAG 
     2341 GCTCCTCCTC CCTTCCCGGC CGCCCGAGTG TGATTCCAGA TCACTCCAAG AAGAACATCG 
     2401 GCAATGGGAT TATTTCCTCC CCACTGACTG GAAAGCGACA AGACTCTGGG ACGATGAAGA 
     2461 AGCCGCACAC CACTGAAGAG ATTGGTGTGC CCATATCCAG GAATGGCTCC ACCCTGGGCC 
     2521 TGAAGTCCCA GAACGGCTGC ATTCCTCCAA AGCTGCCCTC GGGGTCCCCT TCCCCCAAAC 
     2581 TCTCCCAGAC ACCCACACAC ATGCCAACCA TCCTAGACGA CCCTGGAAAG AAGGTGAAGA 
     2641 AGCCAGCTCC TCCACAGCAC TTTTCCCCCA GAACTGCTCA GGGGCTGCCT GGGACCAGCA 
     2701 ACTCGAATAG CAGCAGATCT GGGAGCCAAA GGCAGGGCTC CTGGGACAGC AGGGATGTTG 
     2761 TCCTCTCTAC CTCACCTAAG CTCCTGGCTA CAGCCACTGC CAACGGGCAT GGGCTGAAGG 
     2821 GGAACGACGA GAGCGCTGGC CTCGACAGGA GGGGCTCCAG CAGCTCCAGC CCAGAGCACT 
     2881 CGGCCAGCAG TGACTCCACC AAGGCCCCCC AGACCCCCAG GAGTGGAGCG GCCCATCTCT 
     2941 GCGATTCTCA GGAAACGAAC TGTTCCACCG CTGGCCACTC CAAAACGCCG CCAAGTGGAG 
     3001 CAGATTCTAA GACGGTGAAG CTGAAGTCCC CTGTCCTGAG CAACACCACC ACTGAGCCTG 
     3061 CAAGCACCAT GTCTCCTCCA CCAGCCAAAA AACTGGCCCT TTCTGCCAAG AAGGCCAGCA 
     3121 CCCTGTGGAG GGCGACCGGC AATGACCTCC GTCCACCTCC CCCCTCACCA TCCTCCGACC 
     3181 TCACCCACCC CATGAAAACC TCTCACCCCG TCGTTGCCTC CACTTGGCCC GTCCATAGAG 
     3241 CCAGGGCTGT GTCACCTGCT CCCCAATCAT CCAGCCGCCT GCAACCCCCC TTCAGCCCCC 
     3301 ACCCCACATT GCTGTCCAGT ACCCCCAAGC CCCCAGGGAC GTCAGAACCA CGGAGCTGCT 
     3361 CCTCCATCTC GACGGCGCTG CCTCAGGTCA ACGAGGACCT TGTGTCTCTT CCACACCAGT 
     3421 TGCCAGAGGC CAGTGAGCCC CCCCAGAGCC CCTCTGAGAA GAGGAAAAAG ACCTTTGTGG 
     3481 GAGAGCCGCA GAGGCTGGGC TCAGAGACGC GCCTCCCACA GCACATCAGG GAGGCCACTG 
     3541 CGGCTCCCCA CGGGAAGAGG AAGAGGAAGA AGAAGAAGCG CCCGGAGGAC ACAGCTGCCA 
     3601 GCGCCCTGCA GGAGGGGCAG ACACAGAGAC AGCCTGGGAG CCCCATGTAC AGGAGGGAGG 
     3661 GCCAGGCACA GCTGCCCGCT GTCAGACGGC AGGAAGATGG CACACAGCCA CAGGTGAATG 
     3721 GCCAGCAGGT GGGATGTGTT ACGGACGGCC ACCACGCGAG CAGCAGGAAG CGGAGGAGGA 
     3781 AAGGAGCAGA AGGTCTTGGT GAAGAAGGCG GCCTGCACCA GGACCCACTT CGGCACAGCT 
     3841 GCTCTCCCAT GGGTGATGGT GATCCAGAGG CCATGGAAGA GTCTCCAAGG AAAAAGAAAA 
     3901 AGAAAAAAAG AAAGCAGGAG ACACAGCGGG CAGTAGAAGA GGATGGGCAT CTCAAATGCC 
     3961 CAAGGAGTGC CAAGCCCCAA GATGCTGTTG TCCCCGAGTC CAGCAGCTGC GCACCATCCG 
     4021 CGAATGGCTG GTGTCCTGGG GACCGCATGG GGCTGAGCCA GGCCCCTCCT GTGTCTTGGA 
     4081 ATGGAGAGCG GGAGTCTGAT GTGGTCCAGG AACTGCTCAA ATACTCATCT GATAAAGCTT 
     4141 ACGGGAGAAA AGTTCTGACC TGGGATGGCA AGATGTCGGC GGTCAGTCAG GATGCTATTG 
     4201 AAGACAGCAG ACAGGCCCGG ACTGAGACCG TGGTTGATGA CTGGGACGAA GAGTTTGACC 
     4261 GAGGGAAGGA AAAGAAAATT AAAAAATTTA AGAGAGAGAA GAGGAGAAAC TTCAACGCCT 
     4321 TCCAGAAACT TCAGACTCGA CGGAACTTCT GGTCTGTGAC TCACCCAGCA AAGGCTGCCA 
     4381 GCCTCAGCTA TCGCCGCAGC GGACCGACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4441 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4501 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4561 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4621 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4681 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4741 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4801 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4861 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4921 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4981 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5041 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5101 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5161 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5221 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5281 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5341 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5401 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5461 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5521 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5581 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5641 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5701 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5761 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5821 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5881 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     5941 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6001 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6061 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6121 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6181 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6241 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6301 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6361 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6421 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6481 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6541 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6601 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6661 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6721 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6781 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6841 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6901 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     6961 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7021 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7081 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7141 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7201 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7261 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7321 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7381 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7441 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7501 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7561 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7621 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7681 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7741 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7801 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7861 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7921 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     7981 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8041 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8101 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8161 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8221 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//