LOCUS       RC209777L4                9062 bp    DNA     circular  SYN 
DEFINITION  MFSD5 (Myc-DDK-tagged)-Human major facilitator superfamily domain containing 5 (MFSD5), transcript variant 2
ACCESSION   NM_032889
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7353..7533 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8261..8907 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    6228..6248 
                     /label="LR50"  
     misc_feature    4691..5419 
                     /label="mGFP"  
     misc_feature    5426..5491 
                     /label="P2A"  
     misc_feature    7581..8200 
                     /label="pBR322 Ori"  
     misc_feature    5492..6091 
                     /label="Puro"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    3221..3237 
                     /label="V2"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    4670..4676 
                     /label="Restriction Enzyme"  
     misc_feature    3320..4670 
                     /label="MFSD5"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GCTGGTGACT GCCTACCTTG CTTTTGTAGG 
     3361 CCTCCTGGCC TCCTGCCTGG GGCTGGAACT GTCAAGATGC CGGGCTAAAC CCCCTGGAAG 
     3421 GGCCTGCAGC AATCCCTCCT TCCTTCGGTT TCAACTGGAC TTCTATCAGG TCTACTTCCT 
     3481 GGCCCTGGCA GCTGATTGGC TTCAGGCCCC CTACCTCTAT AAACTCTACC AGCATTACTA 
     3541 CTTCCTGGAA GGTCAAATTG CCATCCTCTA TGTCTGTGGC CTTGCCTCTA CAGTCCTCTT 
     3601 TGGCCTAGTG GCCTCCTCAC TTGTGGATTG GCTGGGTCGC AAGAATTCTT GTGTCCTCTT 
     3661 CTCCCTGACT TACTCACTAT GCTGCTTAAC CAAACTCTCT CAAGACTACT TTGTGATGCT 
     3721 AGTGGGGCGA GCACTTGGTG GGCTGTCCAC AGCCCTGCTC TTCTCAGCCT TCGAGGCCTG 
     3781 GTATATCCAT GAGCACGTGG AACGGCATGA CTTCCCTGCT GAGTGGATCC CAGCTACCTT 
     3841 TGCTCGAGCT GCCTTCTGGA ACCATGTGCT GGCTGTAGTG GCAGGTGTGG CAGCTGAGGC 
     3901 TGTAGCCAGC TGGATAGGGC TGGGGCCTGT AGCGCCCTTT GTGGCTGCCA TCCCTCTCCT 
     3961 GGCTCTGGCA GGGGCCTTGG CCCTTCGAAA CTGGGGGGAG AACTATGACC GGCAGCGTGC 
     4021 CTTCTCAAGG ACCTGTGCTG GAGGCCTGCG CTGCCTCCTG TCGGACCGCC GCGTGCTGCT 
     4081 GTTGGGCACC ATACAAGCTC TATTTGAGAG TGTCATCTTC ATCTTTGTCT TCCTCTGGAC 
     4141 ACCTGTGCTG GACCCACACG GGGCCCCTCT GGGCATTATC TTCTCCAGCT TCATGGCAGC 
     4201 CAGCCTGCTT GGCTCTTCCC TGTACCGTAT CGCCACCTCC AAGAGGTACC ACCTTCAGCC 
     4261 CATGCACCTG CTGTCCCTTG CTGTGCTCAT CGTCGTCTTC TCTCTCTTCA TGTTGACTTT 
     4321 CTCTACCAGC CCAGGCCAGG AGAGTCCGGT GGAGTCCTTC ATAGCCTTTC TACTTATTGA 
     4381 GTTGGCTTGT GGATTATACT TTCCCAGCAT GAGCTTCCTA CGGAGAAAGG TGATCCCTGA 
     4441 GACAGAGCAG GCTGGTGTAC TCAACTGGTT CCGGGTACCT CTGCACTCAC TGGCTTGCCT 
     4501 AGGGCTCCTT GTCCTCCATG ACAGTGATCG AAAAACAGGC ACTCGGAATA TGTTCAGCAT 
     4561 TTGCTCTGCT GTCATGGTGA TGGCTCTGCT GGCAGTGGTG GGACTCTTCA CCGTGGTAAG 
     4621 GCATGATGCT GAGCTGCGGG TACCTTCACC TACTGAGGAG CCCTATGCCC CTGAGCTGAC 
     4681 GCGTACGCGG CCGCTCGAGA TGAGCGGGGG CGAGGAGCTG TTCGCCGGCA TCGTGCCCGT 
     4741 GCTGATCGAG CTGGACGGCG ACGTGCACGG CCACAAGTTC AGCGTGCGCG GCGAGGGCGA 
     4801 GGGCGACGCC GACTACGGCA AGCTGGAGAT CAAGTTCATC TGCACCACCG GCAAGCTGCC 
     4861 CGTGCCCTGG CCCACCCTGG TGACCACCCT CTGCTACGGC ATCCAGTGCT TCGCCCGCTA 
     4921 CCCCGAGCAC ATGAAGATGA ACGACTTCTT CAAGAGCGCC ATGCCCGAGG GCTACATCCA 
     4981 GGAGCGCACC ATCCAGTTCC AGGACGACGG CAAGTACAAG ACCCGCGGCG AGGTGAAGTT 
     5041 CGAGGGCGAC ACCCTGGTGA ACCGCATCGA GCTGAAGGGC AAGGACTTCA AGGAGGACGG 
     5101 CAACATCCTG GGCCACAAGC TGGAGTACAG CTTCAACAGC CACAACGTGT ACATCCGCCC 
     5161 CGACAAGGCC AACAACGGCC TGGAGGCTAA CTTCAAGACC CGCCACAACA TCGAGGGCGG 
     5221 CGGCGTGCAG CTGGCCGACC ACTACCAGAC CAACGTGCCC CTGGGCGACG GCCCCGTGCT 
     5281 GATCCCCATC AACCACTACC TGAGCACTCA GACCAAGATC AGCAAGGACC GCAACGAGGC 
     5341 CCGCGACCAC ATGGTGCTCC TGGAGTCCTT CAGCGCCTGC TGCCACACCC ACGGCATGGA 
     5401 CGAGCTGTAC AGGTCCGGAC TCAGAGTTTG GGTAGGAAGC GGAGCTACTA ACTTCAGCCT 
     5461 GCTGAAGCAG GCTGGAGACG TGGAGGAGAA CCCTGGACCT ATGACCGAGT ACAAGCCCAC 
     5521 GGTGCGCCTC GCCACCCGCG ACGACGTCCC CAGGGCCGTA CGCACCCTCG CCGCCGCGTT 
     5581 CGCCGACTAC CCCGCCACGC GCCACACCGT CGATCCGGAT CGCCACATCG AGCGGGTCAC 
     5641 CGAGCTGCAA GAACTCTTCC TCACGCGCGT CGGGCTCGAC ATCGGCAAGG TGTGGGTCGC 
     5701 GGACGACGGC GCCGCGGTGG CGGTCTGGAC CACGCCGGAG AGCGTCGAAG CGGGGGCGGT 
     5761 GTTCGCCGAG ATCGGCCCGC GCATGGCCGA GTTGAGCGGT TCCCGGCTGG CCGCGCAGCA 
     5821 ACAGATGGAA GGCCTCCTGG CGCCGCACCG GCCCAAGGAG CCCGCGTGGT TCCTGGCCAC 
     5881 CGTCGGCGTC TCGCCCGACC ACCAGGGCAA GGGTCTGGGC AGCGCCGTCG TGCTCCCCGG 
     5941 AGTGGAGGCG GCCGAGCGCG CCGGGGTGCC CGCCTTCCTG GAGACCTCCG CGCCCCGCAA 
     6001 CCTCCCCTTC TACGAGCGGC TCGGCTTCAC CGTCACCGCC GACGTCGAGG TGCCCGAAGG 
     6061 ACCGCGCACC TGGTGCATGA CCCGCAAGCC CGGTGCCTGA TGTACACCCA AACGGCCGGC 
     6121 CGCGGTCTGT ACAAGTAGGA TTCGTCGAGG GACCTAATAA CTTCGTATAG CATACATTAT 
     6181 ACGAAGTTAT ACATGTTTAA GGGTTCCGGT TCCACTAGGT ACAATTCGAT ATCAAGCTTA 
     6241 TCGATAATCA ACCTCTGGAT TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG 
     6301 TTGCTCCTTT TACGCTATGT GGATACGCTG CTTTAATGCC TTTGTATCAT GCTATTGCTT 
     6361 CCCGTATGGC TTTCATTTTC TCCTCCTTGT ATAAATCCTG GTTGCTGTCT CTTTATGAGG 
     6421 AGTTGTGGCC CGTTGTCAGG CAACGTGGCG TGGTGTGCAC TGTGTTTGCT GACGCAACCC 
     6481 CCACTGGTTG GGGCATTGCC ACCACCTGTC AGCTCCTTTC CGGGACTTTC GCTTTCCCCC 
     6541 TCCCTATTGC CACGGCGGAA CTCATCGCCG CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC 
     6601 GGCTGTTGGG CACTGACAAT TCCGTGGTGT TGTCGGGGAA ATCATCGTCC TTTCCTTGGC 
     6661 TGCTCGCCTG TGTTGCCACC TGGATTCTGC GCGGGACGTC CTTCTGCTAC GTCCCTTCGG 
     6721 CCCTCAATCC AGCGGACCTT CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC 
     6781 GTCTTCGCCT TCGCCCTCAG ACGAGTCGGA TCTCCCTTTG GGCCGCCTCC CCGCATCGAT 
     6841 ACCGTCGACC TCGATCGAGA CCTAGAAAAA CATGGAGCAA TCACAAGTAG CAATACAGCA 
     6901 GCTACCAATG CTGATTGTGC CTGGCTAGAA GCACAAGAGG AGGAGGAGGT GGGTTTTCCA 
     6961 GTCACACCTC AGGTACCTTT AAGACCAATG ACTTACAAGG CAGCTGTAGA TCTTAGCCAC 
     7021 TTTTTAAAAG AAAAGGGGGG ACTGGAAGGG CTAATTCACT CCCAACGAAG ACAAGATATC 
     7081 CTTGATCTGT GGATCTACCA CACACAAGGC TACTTCCCTG ATTGGCAGAA CTACACACCA 
     7141 GGGCCAGGGA TCAGATATCC ACTGACCTTT GGATGGTGCT ACAAGCTAGT ACCAGTTGAG 
     7201 CAAGAGAAGG TAGAAGAAGC CAATGAAGGA GAGAACACCC GCTTGTTACA CCCTGTGAGC 
     7261 CTGCATGGGA TGGATGACCC GGAGAGAGAA GTATTAGAGT GGAGGTTTGA CAGCCGCCTA 
     7321 GCATTTCATC ACATGGCCCG AGAGCTGCAT CCGGACTGTA CTGGGTCTCT CTGGTTAGAC 
     7381 CAGATCTGAG CCTGGGAGCT CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA 
     7441 AGCTTGCCTT GAGTGCTTCA AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG 
     7501 AGATCCCTCA GACCCTTTTA GTCAGTGTGG AAAATCTCTA GCAGCATGTG AGCAAAAGGC 
     7561 CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC 
     7621 CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA 
     7681 CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC 
     7741 CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT 
     7801 AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG 
     7861 CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC 
     7921 AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA 
     7981 GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT 
     8041 AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT 
     8101 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG 
     8161 CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG 
     8221 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAT TACGCCCCGC 
     8281 CCTGCCACTC ATCGCAGTAC TGTTGTAATT CATTAAGCAT TCTGCCGACA TGGAAGCCAT 
     8341 CACAAACGGC ATGATGAACC TGAATCGCCA GCGGCATCAG CACCTTGTCG CCTTGCGTAT 
     8401 AATATTTGCC CATGGTGAAA ACGGGGGCGA AGAAGTTGTC CATATTGGCC ACGTTTAAAT 
     8461 CAAAACTGGT GAAACTCACC CAGGGATTGG CTGAGACGAA AAACATATTC TCAATAAACC 
     8521 CTTTAGGGAA ATAGGCCAGG TTTTCACCGT AACACGCCAC ATCTTGCGAA TATATGTGTA 
     8581 GAAACTGCCG GAAATCGTCG TGGTATTCAC TCCAGAGCGA TGAAAACGTT TCAGTTTGCT 
     8641 CATGGAAAAC GGTGTAACAA GGGTGAACAC TATCCCATAT CACCAGCTCA CCGTCTTTCA 
     8701 TTGCCATACG GAACTCCGGA TGAGCATTCA TCAGGCGGGC AAGAATGTGA ATAAAGGCCG 
     8761 GATAAAACTT GTGCTTATTT TTCTTTACGG TCTTTAAAAA GGCCGTAATA TCCAGCTGAA 
     8821 CGGTCTGGTT ATAGGTACAT TGAGCAACTG ACTGAAATGC CTCAAAATGT TCTTTACGAT 
     8881 GCCATTGGGA TATATCAACG GTGGTATATC CAGTGATTTT TTTCTCCATA CTCTTCCTTT 
     8941 TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT 
     9001 GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG 
     9061 AC
//