LOCUS       RC209685L4                8761 bp    DNA     circular  SYN 
DEFINITION  GPR21 (Myc-DDK-tagged)-Human G protein-coupled receptor 21 (GPR21)
ACCESSION   NM_005294
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7052..7232 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    7960..8606 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    5927..5947 
                     /label="LR50"  
     misc_feature    4390..5118 
                     /label="mGFP"  
     misc_feature    5125..5190 
                     /label="P2A"  
     misc_feature    7280..7899 
                     /label="pBR322 Ori"  
     misc_feature    5191..5790 
                     /label="Puro"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    3221..3237 
                     /label="V2"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    4369..4375 
                     /label="Restriction Enzyme"  
     misc_feature    3320..4369 
                     /label="GPR21"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GAACTCCACC TTGGATGGTA ATCAGAGCAG 
     3361 CCACCCTTTT TGCCTCTTGG CATTTGGCTA TTTGGAAACT GTCAATTTTT GCCTTTTGGA 
     3421 AGTATTGATT ATTGTCTTTC TAACTGTATT GATTATTTCT GGCAACATCA TTGTGATTTT 
     3481 TGTATTTCAC TGTGCACCTT TGTTGAACCA TCACACTACA AGTTATTTTA TCCAGACTAT 
     3541 GGCATATGCT GACCTTTTTG TTGGGGTGAG CTGCGTGGTC CCTTCTTTAT CACTCCTCCA 
     3601 TCACCCCCTT CCAGTAGAGG AGTCCTTGAC TTGCCAGATA TTTGGTTTTG TAGTATCAGT 
     3661 TCTGAAGAGC GTCTCCATGG CTTCTCTGGC CTGTATCAGC ATTGATAGAT ACATTGCCAT 
     3721 TACTAAACCT TTAACCTATA ATACTCTGGT TACACCCTGG AGACTACGCC TGTGTATTTT 
     3781 CCTGATTTGG CTATACTCGA CCCTGGTCTT CCTGCCTTCC TTTTTCCACT GGGGCAAACC 
     3841 TGGATATCAT GGAGATGTGT TTCAGTGGTG TGCGGAGTCC TGGCACACCG ACTCCTACTT 
     3901 CACCCTGTTC ATCGTGATGA TGTTATATGC CCCAGCAGCC CTTATTGTCT GCTTCACCTA 
     3961 TTTCAACATC TTCCGCATCT GCCAACAGCA CACAAAGGAT ATCAGCGAAA GGCAAGCCCG 
     4021 CTTCAGCAGC CAGAGTGGGG AGACTGGGGA AGTGCAGGCC TGTCCTGATA AGCGCTATGC 
     4081 CATGGTCCTG TTTCGAATCA CTAGTGTATT TTACATCCTC TGGTTGCCAT ATATCATCTA 
     4141 CTTCTTGTTG GAAAGCTCCA CTGGCCACAG CAACCGCTTC GCATCCTTCT TGACCACCTG 
     4201 GCTTGCTATT AGTAACAGTT TCTGCAACTG TGTAATTTAT AGTCTCTCCA ACAGTGTATT 
     4261 CCAAAGAGGA CTAAAGCGCC TCTCAGGGGC TATGTGTACT TCTTGTGCAA GTCAGACTAC 
     4321 AGCCAACGAC CCTTACACAG TTAGAAGCAA AGGCCCTCTT AATGGATGTC ATATCTGACG 
     4381 CGTACGCGGC CGCTCGAGAT GAGCGGGGGC GAGGAGCTGT TCGCCGGCAT CGTGCCCGTG 
     4441 CTGATCGAGC TGGACGGCGA CGTGCACGGC CACAAGTTCA GCGTGCGCGG CGAGGGCGAG 
     4501 GGCGACGCCG ACTACGGCAA GCTGGAGATC AAGTTCATCT GCACCACCGG CAAGCTGCCC 
     4561 GTGCCCTGGC CCACCCTGGT GACCACCCTC TGCTACGGCA TCCAGTGCTT CGCCCGCTAC 
     4621 CCCGAGCACA TGAAGATGAA CGACTTCTTC AAGAGCGCCA TGCCCGAGGG CTACATCCAG 
     4681 GAGCGCACCA TCCAGTTCCA GGACGACGGC AAGTACAAGA CCCGCGGCGA GGTGAAGTTC 
     4741 GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAAGGGCA AGGACTTCAA GGAGGACGGC 
     4801 AACATCCTGG GCCACAAGCT GGAGTACAGC TTCAACAGCC ACAACGTGTA CATCCGCCCC 
     4861 GACAAGGCCA ACAACGGCCT GGAGGCTAAC TTCAAGACCC GCCACAACAT CGAGGGCGGC 
     4921 GGCGTGCAGC TGGCCGACCA CTACCAGACC AACGTGCCCC TGGGCGACGG CCCCGTGCTG 
     4981 ATCCCCATCA ACCACTACCT GAGCACTCAG ACCAAGATCA GCAAGGACCG CAACGAGGCC 
     5041 CGCGACCACA TGGTGCTCCT GGAGTCCTTC AGCGCCTGCT GCCACACCCA CGGCATGGAC 
     5101 GAGCTGTACA GGTCCGGACT CAGAGTTTGG GTAGGAAGCG GAGCTACTAA CTTCAGCCTG 
     5161 CTGAAGCAGG CTGGAGACGT GGAGGAGAAC CCTGGACCTA TGACCGAGTA CAAGCCCACG 
     5221 GTGCGCCTCG CCACCCGCGA CGACGTCCCC AGGGCCGTAC GCACCCTCGC CGCCGCGTTC 
     5281 GCCGACTACC CCGCCACGCG CCACACCGTC GATCCGGATC GCCACATCGA GCGGGTCACC 
     5341 GAGCTGCAAG AACTCTTCCT CACGCGCGTC GGGCTCGACA TCGGCAAGGT GTGGGTCGCG 
     5401 GACGACGGCG CCGCGGTGGC GGTCTGGACC ACGCCGGAGA GCGTCGAAGC GGGGGCGGTG 
     5461 TTCGCCGAGA TCGGCCCGCG CATGGCCGAG TTGAGCGGTT CCCGGCTGGC CGCGCAGCAA 
     5521 CAGATGGAAG GCCTCCTGGC GCCGCACCGG CCCAAGGAGC CCGCGTGGTT CCTGGCCACC 
     5581 GTCGGCGTCT CGCCCGACCA CCAGGGCAAG GGTCTGGGCA GCGCCGTCGT GCTCCCCGGA 
     5641 GTGGAGGCGG CCGAGCGCGC CGGGGTGCCC GCCTTCCTGG AGACCTCCGC GCCCCGCAAC 
     5701 CTCCCCTTCT ACGAGCGGCT CGGCTTCACC GTCACCGCCG ACGTCGAGGT GCCCGAAGGA 
     5761 CCGCGCACCT GGTGCATGAC CCGCAAGCCC GGTGCCTGAT GTACACCCAA ACGGCCGGCC 
     5821 GCGGTCTGTA CAAGTAGGAT TCGTCGAGGG ACCTAATAAC TTCGTATAGC ATACATTATA 
     5881 CGAAGTTATA CATGTTTAAG GGTTCCGGTT CCACTAGGTA CAATTCGATA TCAAGCTTAT 
     5941 CGATAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT 
     6001 TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC 
     6061 CCGTATGGCT TTCATTTTCT CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA 
     6121 GTTGTGGCCC GTTGTCAGGC AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC 
     6181 CACTGGTTGG GGCATTGCCA CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT 
     6241 CCCTATTGCC ACGGCGGAAC TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG 
     6301 GCTGTTGGGC ACTGACAATT CCGTGGTGTT GTCGGGGAAA TCATCGTCCT TTCCTTGGCT 
     6361 GCTCGCCTGT GTTGCCACCT GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC 
     6421 CCTCAATCCA GCGGACCTTC CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG 
     6481 TCTTCGCCTT CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC CGCATCGATA 
     6541 CCGTCGACCT CGATCGAGAC CTAGAAAAAC ATGGAGCAAT CACAAGTAGC AATACAGCAG 
     6601 CTACCAATGC TGATTGTGCC TGGCTAGAAG CACAAGAGGA GGAGGAGGTG GGTTTTCCAG 
     6661 TCACACCTCA GGTACCTTTA AGACCAATGA CTTACAAGGC AGCTGTAGAT CTTAGCCACT 
     6721 TTTTAAAAGA AAAGGGGGGA CTGGAAGGGC TAATTCACTC CCAACGAAGA CAAGATATCC 
     6781 TTGATCTGTG GATCTACCAC ACACAAGGCT ACTTCCCTGA TTGGCAGAAC TACACACCAG 
     6841 GGCCAGGGAT CAGATATCCA CTGACCTTTG GATGGTGCTA CAAGCTAGTA CCAGTTGAGC 
     6901 AAGAGAAGGT AGAAGAAGCC AATGAAGGAG AGAACACCCG CTTGTTACAC CCTGTGAGCC 
     6961 TGCATGGGAT GGATGACCCG GAGAGAGAAG TATTAGAGTG GAGGTTTGAC AGCCGCCTAG 
     7021 CATTTCATCA CATGGCCCGA GAGCTGCATC CGGACTGTAC TGGGTCTCTC TGGTTAGACC 
     7081 AGATCTGAGC CTGGGAGCTC TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA 
     7141 GCTTGCCTTG AGTGCTTCAA GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA 
     7201 GATCCCTCAG ACCCTTTTAG TCAGTGTGGA AAATCTCTAG CAGCATGTGA GCAAAAGGCC 
     7261 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC 
     7321 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC 
     7381 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC 
     7441 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA 
     7501 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC 
     7561 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA 
     7621 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG 
     7681 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA 
     7741 GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG 
     7801 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC 
     7861 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT 
     7921 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGATT ACGCCCCGCC 
     7981 CTGCCACTCA TCGCAGTACT GTTGTAATTC ATTAAGCATT CTGCCGACAT GGAAGCCATC 
     8041 ACAAACGGCA TGATGAACCT GAATCGCCAG CGGCATCAGC ACCTTGTCGC CTTGCGTATA 
     8101 ATATTTGCCC ATGGTGAAAA CGGGGGCGAA GAAGTTGTCC ATATTGGCCA CGTTTAAATC 
     8161 AAAACTGGTG AAACTCACCC AGGGATTGGC TGAGACGAAA AACATATTCT CAATAAACCC 
     8221 TTTAGGGAAA TAGGCCAGGT TTTCACCGTA ACACGCCACA TCTTGCGAAT ATATGTGTAG 
     8281 AAACTGCCGG AAATCGTCGT GGTATTCACT CCAGAGCGAT GAAAACGTTT CAGTTTGCTC 
     8341 ATGGAAAACG GTGTAACAAG GGTGAACACT ATCCCATATC ACCAGCTCAC CGTCTTTCAT 
     8401 TGCCATACGG AACTCCGGAT GAGCATTCAT CAGGCGGGCA AGAATGTGAA TAAAGGCCGG 
     8461 ATAAAACTTG TGCTTATTTT TCTTTACGGT CTTTAAAAAG GCCGTAATAT CCAGCTGAAC 
     8521 GGTCTGGTTA TAGGTACATT GAGCAACTGA CTGAAATGCC TCAAAATGTT CTTTACGATG 
     8581 CCATTGGGAT ATATCAACGG TGGTATATCC AGTGATTTTT TTCTCCATAC TCTTCCTTTT 
     8641 TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG 
     8701 TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA 
     8761 C
//