LOCUS       RC209524                8277 bp    DNA     circular  SYN 
DEFINITION  MIS18BP1 (Myc-DDK-tagged)-Human MIS18 binding protein 1 (MIS18BP1)
ACCESSION   NM_018353
VERSION     NM_018353.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4434..4525 
                     /label="Myc-DDK"  
     misc_feature    4555..5140 
                     /label="PolyA signal"  
     misc_feature    4612..4631 
                     /label="Restriction Enzyme"  
     misc_feature    5416..6088 
                     /label="ColE1"  
     misc_feature    6518..7312 
                     /label="Kan/Neo"  
     misc_feature    7347..7649 
                     /label="SV40 ori"  
     misc_feature    7735..8212 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4416..4422 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4416 
                     /label="MIS18BP1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATTGCAACA CCTTTGAAAC ATTCAAGAAT TTACTTACCT CCAGAGGCAT 
     1081 CTTCTCAAAG GAGAAATCTA CCCATGGATG CAATCTTTTT TGACAGCATT CCTTCAGGCA 
     1141 CACTTACTCC TGTAAAAGAT TTGGTGAAAT ATCAGAACTC CTCCTTAAAA TTGAATGACC 
     1201 ATAAAAAGAA TCAGTTCCTA AAAATGACAA CTTTTAACAA TAAAAATATA TTTCAATCAA 
     1261 CTATGCTAAC AGAGGCTACT ACCTCTAACA GTTCTCTTGA TATCAGTGCT ATAAAGCCCA 
     1321 ACAAGGATGG ATTAAAAAAT AAAGCAAACT ATGAATCACC AGGAAAAATA TTTCTAAGAA 
     1381 TGAAAGAAAA AGTACTGCGT GACAAGCAAG AACAGCCATC AAGAAACAGT AGTTTGTTGG 
     1441 AACCACAGAA AAGTGGAAAT AATGAAACCT TCACTCCTAA CAGAGTTGAA AAAAAAAAAT 
     1501 TGCAGCATAC CTACCTATGT GAAGAAAAGG AAAACAACAA ATCATTCCAG TCAGATGACA 
     1561 GTTCACTAAG AGCCTCAGTC CAAGGAGTTC CTCTAGAATC ATCAAATAAT GATATTTTCC 
     1621 TCCCGGTCAA ACAAAAGATT CAGTGCCAGC AGGAAAAGAA AGCACCACTG CACAATTTAA 
     1681 CTTACGAACT TCCAACTCTG AACCAAGAAC AGGAAAATTT TTTGGCTGTA GAAGCCCGAA 
     1741 ACAAGACATT AACTAGAGCT CAGTTGGCTA AACAAATTTT TCACTCAAAG GAGAGTATAG 
     1801 TTGCAACCAC TAAATCCAAA AAGGACACGT TTGTTTTAGA AAGCGTTGAT TCTGCTGATG 
     1861 AACAATTTCA AAATACTAAT GCTGAGACTC TCAGTACTAA TTGTATTCCT ATTAAAAATG 
     1921 GCAGCCTGTT AATGGTTTCT GATAGTGAGA GGACAACAGA AGGGACTTCG CAACAGAAAG 
     1981 TTAAGGAAGG AAATGGAAAA ACAGTGCCTG GAGAGACAGG TCTTCCAGGT TCCATGAAAG 
     2041 ATACATGTAA AATTGTACTT GCAACACCAA GACTTCATAT AACAATACCT CGGAGGTCAA 
     2101 AAAGAAATAT TTCAAAGCTT TCTCCTCCAA GAATATTTCA AACTGTTACA AATGGACTTA 
     2161 AAAAAAATCA GGTAGTTCAG CTACAGGAAT GGATGATTAA AAGCATCAAT AATAATACTG 
     2221 CTATATGTGT AGAAGGAAAA TTGATAGACG TCACTAACAT ATATTGGCAC AGTAATGTAA 
     2281 TTATAGAGCG GATTGAGCAC AACAAACTTA GGACTATATC AGGCAACGTT TATATATTAA 
     2341 AAGGCATGAT AGACCAAATT TCCATGAAAG AAGCAGGATA TCCAAATTAT CTCATAAGGA 
     2401 AATTTATGTT TGGATTTCCA GAAAATTGGA AAGAGCACAT TGATAATTTT CTGGAACAAT 
     2461 TAAGGGCTGG TGAAAAGAAC AGGGAAAAGA CCAAACAAAA ACAGAAAACT GGAAGATCTG 
     2521 TCCGTGACAT AAGGAAATCA ATGAAAAATG ATGCACGAGA AAACCAAACA GATACTGCTC 
     2581 AAAGAGCCAC CACCACTTAC GATTTTGATT GTGATAATTT GGAACTGAAG AGTAATAAGC 
     2641 ACAGTGAGTC ACCAGGAGCT ACAGAATTAA ACATGTGCCA CAGTAATTGC CAAAATAAAC 
     2701 CAACATTAAG GTTCCCAGAT GACCAAGTAA ATAATACTAT TCAAAATGGA GGAGGAGATG 
     2761 ACTTATCTAA TCAGGAATTA ATTGGAAAAA AAGAATATAA AATGTCTTCA AAGAAACTAA 
     2821 AAATTGGTGA AAGAACAAAT GAAAGGATAA TAAAAAGTCA GAAGCAAGAG ACAACTGAAG 
     2881 AATTGGATGT ATCCATTGAT ATTCTAACCT CAAGGGAACA GTTTTTCTCA GATGAAGAAA 
     2941 GAAAATACAT GGCCATCAAT CAGAAGAAAG CTTATATTTT AGTAACACCA CTTAAATCTA 
     3001 GAAAAGTGAT AGAGCAAAGA TGCATGAGGT ATAATCTGTC CGCTGGCACC ATCAAAGCAG 
     3061 TAACAGATTT TGTAATACCA GAGTGTCAAA AAAAAAGTCC CATCAGCAAG TCCATGGGGA 
     3121 CTTTAGAAAA TACATTTGAA GGTCATAAAA GTAAAAACAA GGAAGATTGC GATGAACGTG 
     3181 ACTTACTTAC TGTCAACCGG AAAATAAAAA TATCTAACCT TGAAAAGGAA CAAATGCTCA 
     3241 CCTCTGACTT TAAGAAAAAT ACCAGACTAT TACCAAAATT GAAGAAAATA GAAAATCAGG 
     3301 TAGCTATGTC ATTTTATAAG CATCAGTCCT CACCAGATTT GTCAAGTGAA GAAAGTGAAA 
     3361 CAGAAAAGGA AATTAAAAGG AAAGCTGAAG TTAAGAAAAC CAAAGCAGGA AACACCAAAG 
     3421 AAGCAGTGGT TCACCTGAGA AAGAGCACAA GAAACACAAG TAATATTCCA GTGATTTTGG 
     3481 AACCTGAAAC TGAAGAAAGT GAAAATGAAT TTTATATCAA ACAAAAGAAA GCTAGACCTT 
     3541 CCGTCAAAGA AACTCTTCAG AAGTCTGGTG TTAGGAAAGA GTTTCCAATT ACTGAGGCAG 
     3601 TAGGATCTGA TAAGACAAAT AGGCATCCCT TAGAATGCTT ACCTGGTTTA ATTCAGGATA 
     3661 AGGAATGGAA TGAGAAGGAG TTACAGAAAC TTCATTGTGC TTTTGCATCT CTTCCAAAGC 
     3721 ACAAACCTGG TTTCTGGTCA GAGGTAGCTG CGGCTGTAGG TTCTCGATCT CCTGAAGAAT 
     3781 GCCAGAGGAA ATACATGGAA AATCCCAGAG GAAAAGGATC CCAGAAACAT GTCACTAAGA 
     3841 AGAAGCCAGC CAATTCCAAA GGCCAAAATG GCAAGAGAGG TGATGCTGAT CAGAAACAAA 
     3901 CTATTAAGAT AACTGCCAAA GTGGGAACTC TTAAAAGGAA GCAACAGATG AGGGAATTTC 
     3961 TGGAACAGTT GCCAAAAGAT GACCATGATG ATTTTTTCAG TACAACACCT TTACAGCATC 
     4021 AAAGAATACT GTTGCCAAGT TTCCAGGACA GTGAAGATGA TGATGATATT CTGCCAAATA 
     4081 TGGACAAAAA TCCAACAACT CCATCATCAG TTATCTTTCC ATTGGTAAAA ACTCCTCAAT 
     4141 GTCAGCATGT CAGTCCTGGC ATGCTAGGTT CTATAAATAG GAATGACTGT GATAAATATG 
     4201 TTTTTCGTAT GCAAAAATAT CATAAAAGTA ATGGTGGTAT TGTCTGGGGC AACATCAAGA 
     4261 AAAAATTAGT TGAAACTGAT TTCTCAACTC CAACACCAAG AAGGAAAACC CCATTTAACA 
     4321 CAGACTTAGG AGAAAACTCT GGTATTGGAA AACTTTTCAC TAATGCTGTG GAATCTTTAG 
     4381 ATGAAGAAGA GAAAGATTAT TATTTTTCGA ACTCTGATTC TGCAACGCGT ACGCGGCCGC 
     4441 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4501 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4561 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4621 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4681 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4741 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4801 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4861 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4921 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4981 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5041 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5101 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5161 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5221 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5281 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5341 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5401 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5461 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5521 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5581 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5641 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5701 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5761 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5821 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5881 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5941 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6001 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6061 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6121 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6181 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6241 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6301 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6361 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6421 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6481 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6541 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6601 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6661 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6721 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6781 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6841 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6901 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6961 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7021 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7081 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7141 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7201 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7261 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7321 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7381 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7441 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7501 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7561 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7621 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7681 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7741 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7801 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7861 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7921 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7981 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8041 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8101 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8161 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8221 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//