LOCUS       RC209465                8889 bp    DNA     circular  SYN 
DEFINITION  COL18A1 (Myc-DDK-tagged)-Human collagen, type XVIII, alpha 1 (COL18A1), transcript variant 2
ACCESSION   NM_130445
VERSION     NM_130445.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5046..5137 
                     /label="Myc-DDK"  
     misc_feature    5167..5752 
                     /label="PolyA signal"  
     misc_feature    5224..5243 
                     /label="Restriction Enzyme"  
     misc_feature    6028..6700 
                     /label="ColE1"  
     misc_feature    7130..7924 
                     /label="Kan/Neo"  
     misc_feature    7959..8261 
                     /label="SV40 ori"  
     misc_feature    8347..8824 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5028..5034 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5028 
                     /label="COL18A1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGCCGAGG TGCCCCTGGC CATGGCCGCG GCGGCGGCGC CTCCTGGACG 
     1081 TGCTCGCGCC CCTGGTCCTG CTGCTCGGGG TCCGCGCGGC CTCCGCGGAG CCAGAGCGCA 
     1141 TCAGCGAGGA GGTGGGGCTG CTGCAGCTCC TTGGGGACCC CCCGCCCCAG CAGGTCACCC 
     1201 AGACGGATGA CCCCGACGTC GGGCTGGCCT ACGTCTTTGG GCCAGATGCC AACAGTGGCC 
     1261 AAGTGGCCCG GTACCACTTC CCCAGCCTCT TCTTCCGTGA CTTCTCACTG CTGTTCCACA 
     1321 TCCGGCCAGC CACAGAGGGC CCAGGGGTGC TGTTCGCCAT CACGGACTCG GCGCAGGCCA 
     1381 TGGTCTTGCT GGGCGTGAAG CTCTCTGGGG TGCAGGACGG GCACCAGGAC ATCTCCCTGC 
     1441 TCTACACAGA ACCAGGTGCA GGCCAGACCC ACACAGCCGC CAGCTTCCGG CTCCCCGCCT 
     1501 TCGTCGGCCA GTGGACACAC TTAGCCCTCA GTGTGGCAGG TGGCTTTGTG GCCCTCTACG 
     1561 TGGACTGTGA GGAGTTCCAG AGAATGCCGC TTGCTCGGTC CTCACGGGGC CTGGAGCTGG 
     1621 AGCCTGGCGC CGGGCTCTTC GTGGCTCAGG CGGGGGGAGC GGACCCTGAC AAGTTCCAGG 
     1681 GGGTGATCGC TGAGCTGAAG GTGCGCAGGG ACCCCCAGGT GAGCCCCATG CACTGCCTGG 
     1741 ACGAGGAAGG CGATGACTCA GATGGGGCAT CCGGAGACTC TGGCAGCGGG CTCGGGGACG 
     1801 CCCGGGAGCT TCTCAGGGAG GAGACGGGCG CGGCCCTAAA ACCCAGGCTC CCCGCGCCAC 
     1861 CCCCCGTCAC CACGCCACCC TTGGCTGGAG GCAGCAGCAC GGAAGATTCC AGAAGTGAAG 
     1921 AAGTCGAGGA GCAGACCACG GTGGCTTCGT TAGGAGCTCA GACACTTCCT GGCTCAGATT 
     1981 CTGTCTCCAC GTGGGACGGG AGTGTCCGGA CCCCTGGGGG CCGCGTGAAA GAGGGCGGCC 
     2041 TGAAGGGGCA GAAAGGGGAG CCAGGTGTTC CGGGCCCACC TGGCCGGGCA GGCCCCCCAG 
     2101 GATCCCCATG CCTACCTGGT CCCCCGGGTC TCCCGTGCCC AGTGAGTCCC CTGGGTCCTG 
     2161 CAGGCCCAGC GTTGCAAACT GTCCCCGGAC CACAAGGACC CCCAGGGCCT CCGGGGAGGG 
     2221 ACGGCACCCC TGGAAGGGAC GGCGAGCCGG GCGACCCCGG TGAAGACGGA AAGCCGGGCG 
     2281 ACACCGGGCC ACAAGGCTTC CCCGGGACTC CAGGGGACGT AGGTCCCAAG GGCGACAAGG 
     2341 GAGACCCTGG GGTTGGAGAG AGAGGGCCCC CAGGACCCCA AGGGCCTCCA GGGCCCCCAG 
     2401 GACCCTCCTT CAGACACGAC AAGCTGACCT TCATTGACAT GGAGGGATCT GGCTTCGGGG 
     2461 GCGATCTGGA GGCCCTGCGG GGTCCTCGAG GCTTCCCTGG ACCTCCCGGA CCCCCCGGTG 
     2521 TCCCAGGCCT GCCCGGCGAG CCAGGCCGCT TTGGGGTGAA CAGCTCCGAC GTCCCAGGAC 
     2581 CCGCCGGCCT TCCTGGTGTG CCTGGGCGCG AGGGTCCCCC CGGGTTTCCT GGCCTCCCGG 
     2641 GACCCCCAGG CCCTCCGGGA AGAGAGGGGC CCCCAGGAAG GACTGGGCAG AAAGGCAGCC 
     2701 TGGGTGAAGC AGGCGCCCCA GGACATAAGG GGAGCAAGGG AGCCCCCGGT CCTGCTGGTG 
     2761 CTCGTGGGGA GAGCGGCCTG GCAGGAGCCC CCGGACCTGC TGGACCACCA GGCCCCCCTG 
     2821 GGCCCCCTGG GCCCCCAGGA CCAGGACTCC CCGCTGGATT TGATGACATG GAAGGCTCCG 
     2881 GGGGGCCCTT CTGGTCAACA GCCCGAAGCG CTGATGGGCC ACAGGGACCT CCCGGCCTGC 
     2941 CGGGACTTAA GGGGGATCCT GGCGTGCCTG GGCTGCCGGG GGCGAAGGGA GAAGTTGGAG 
     3001 CAGATGGAGT CCCCGGGTTC CCCGGCCTCC CTGGCAGAGA GGGCATTGCT GGGCCCCAGG 
     3061 GGCCAAAGGG AGACAGAGGC AGCCGGGGAG AAAAGGGAGA TCCAGGGAAG GACGGAGTCG 
     3121 GGCAGCCGGG CCTCCCTGGC CCCCCCGGAC CCCCGGGACC TGTGGTCTAC GTGTCGGAGC 
     3181 AGGACGGATC CGTCCTGAGC GTGCCGGGAC CTGAGGGCCG GCCGGGTTTC GCAGGCTTTC 
     3241 CCGGACCTGC AGGACCCAAG GGCAACCTGG GCTCTAAGGG CGAACGAGGC TCCCCGGGAC 
     3301 CCAAGGGTGA GAAGGGTGAA CCGGGCAGCA TCTTCAGCCC CGACGGCGGT GCCCTGGGCC 
     3361 CTGCCCAGAA AGGAGCCAAG GGAGAGCCGG GCTTCCGAGG ACCCCCGGGT CCATACGGAC 
     3421 GGCCGGGGTA CAAGGGAGAG ATTGGCTTTC CTGGACGGCC GGGTCGCCCC GGGATGAACG 
     3481 GATTGAAAGG AGAGAAAGGG GAGCCGGGAG ATGCCAGCCT TGGATTTGGC ATGAGGGGAA 
     3541 TGCCCGGCCC CCCAGGACCT CCAGGGCCCC CAGGCCCTCC AGGGACTCCT GTTTACGACA 
     3601 GCAATGTGTT TGCTGAGTCC AGCCGCCCCG GGCCTCCAGG ATTGCCAGGG AATCAGGGCC 
     3661 CTCCAGGACC CAAGGGCGCC AAAGGAGAAG TGGGCCCCCC CGGACCACCA GGGCAGTTTC 
     3721 CGTTTGACTT TCTTCAGTTG GAGGCTGAAA TGAAGGGGGA GAAGGGAGAC CGAGGTGATG 
     3781 CAGGACAGAA AGGCGAAAGG GGGGAGCCCG GGGGCGGCGG TTTCTTCGGC TCCAGCCTGC 
     3841 CCGGCCCCCC CGGCCCCCCA GGCCCACGTG GCTACCCTGG GATTCCAGGT CCCAAGGGAG 
     3901 AGAGCATCCG GGGCCAGCCC GGCCCACCTG GACCTCAGGG ACCCCCCGGC ATCGGCTACG 
     3961 AGGGGCGCCA GGGCCCTCCC GGCCCCCCAG GCCCCCCAGG GCCCCCTTCA TTTCCTGGCC 
     4021 CTCACAGGCA GACTATCAGC GTTCCCGGCC CTCCGGGCCC CCCTGGGCCC CCTGGGCCCC 
     4081 CTGGAACCAT GGGCGCCTCC TCAGGGGTGA GGCTCTGGGC TACACGCCAG GCCATGCTGG 
     4141 GCCAGGTGCA CGAGGTTCCC GAGGGCTGGC TCATCTTCGT GGCCGAGCAG GAGGAGCTCT 
     4201 ACGTCCGCGT GCAGAACGGG TTCCGGAAGG TCCAGCTGGA GGCCCGGACA CCACTCCCAC 
     4261 GAGGGACGGA CAATGAAGTG GCCGCCTTGC AGCCCCCCGT GGTGCAGCTG CACGACAGCA 
     4321 ACCCCTACCC GCGGCGGGAG CACCCCCACC CCACCGCGCG GCCCTGGCGG GCAGATGACA 
     4381 TCCTGGCCAG CCCCCCTCGC CTGCCCGAGC CCCAGCCCTA CCCCGGAGCC CCGCACCACA 
     4441 GCTCCTACGT GCACCTGCGG CCGGCACGAC CCACAAGCCC ACCCGCCCAC AGCCACCGCG 
     4501 ACTTCCAGCC GGTGCTCCAC CTGGTTGCGC TCAACAGCCC CCTGTCAGGC GGCATGCGGG 
     4561 GCATCCGCGG GGCCGACTTC CAGTGCTTCC AGCAGGCGCG GGCCGTGGGG CTGGCGGGCA 
     4621 CCTTCCGCGC CTTCCTGTCC TCGCGCCTGC AGGACCTGTA CAGCATCGTG CGCCGTGCCG 
     4681 ACCGCGCAGC CGTGCCCATC GTCAACCTCA AGGACGAGCT GCTGTTTCCC AGCTGGGAGG 
     4741 CTCTGTTCTC AGGCTCTGAG GGTCCGCTGA AGCCCGGGGC ACGCATCTTC TCCTTTGACG 
     4801 GCAAGGACGT CCTGAGGCAC CCCACCTGGC CCCAGAAGAG CGTGTGGCAT GGCTCGGACC 
     4861 CCAACGGGCG CAGGCTGACC GAGAGCTACT GTGAGACGTG GCGGACGGAG GCTCCCTCGG 
     4921 CCACGGGCCA GGCCTCCTCG CTGCTGGGGG GCAGGCTCCT GGGGCAGAGT GCCGCGAGCT 
     4981 GCCATCACGC CTACATCGTG CTCTGCATTG AGAACAGCTT CATGACTGCC TCCAAGACGC 
     5041 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     5101 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     5161 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     5221 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5281 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5341 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5401 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5461 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5521 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5581 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5641 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5701 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5761 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5821 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5881 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5941 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     6001 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     6061 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     6121 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     6181 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6241 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6301 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6361 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6421 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6481 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6541 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6601 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6661 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6721 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6781 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6841 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6901 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6961 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     7021 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     7081 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     7141 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     7201 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7261 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7321 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7381 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7441 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7501 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7561 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7621 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7681 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7741 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7801 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7861 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7921 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7981 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     8041 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     8101 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     8161 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     8221 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8281 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8341 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8401 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8461 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8521 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8581 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8641 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8701 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8761 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8821 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8881 GCGAATTTT
//