LOCUS       RC209460                7854 bp    DNA     circular  SYN 
DEFINITION  RECQL5 (Myc-DDK-tagged)-Human RecQ protein-like 5 (RECQL5), transcript variant 1
ACCESSION   NM_004259
VERSION     NM_004259.5
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4011..4102 
                     /label="Myc-DDK"  
     misc_feature    4132..4717 
                     /label="PolyA signal"  
     misc_feature    4189..4208 
                     /label="Restriction Enzyme"  
     misc_feature    4993..5665 
                     /label="ColE1"  
     misc_feature    6095..6889 
                     /label="Kan/Neo"  
     misc_feature    6924..7226 
                     /label="SV40 ori"  
     misc_feature    7312..7789 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3993..3999 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3993 
                     /label="RECQL5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCAGCCAC CATACCACCT TTCCTTTTGA CCCTGAGCGG CGAGTCCGGA 
     1081 GTACGCTGAA GAAGGTCTTT GGGTTTGACT CTTTTAAGAC GCCTTTACAG GAGAGTGCGA 
     1141 CCATGGCTGT AGTAAAAGGT AACAAGGACG TCTTTGTGTG CATGCCCACA GGGGCAGGAA 
     1201 AATCCCTATG CTATCAGCTC CCTGCTCTGT TGGCCAAAGG CATCACCATT GTAGTCTCTC 
     1261 CTCTCATTGC TTTGATTCAG GACCAAGTGG ACCACTTGCT AACCCTAAAG GTACGAGTAA 
     1321 GTTCCCTGAA CTCGAAGCTC TCTGCACAGG AAAGGAAGGA GCTGCTTGCT GACCTGGAGC 
     1381 GAGAAAAGCC CCAGACCAAG ATTCTGTACA TCACCCCAGA GATGGCAGCT TCATCCTCCT 
     1441 TCCAGCCCAC CCTGAACTCC CTGGTGTCCC GCCACCTGCT GTCTTACTTG GTGGTGGATG 
     1501 AAGCTCATTG TGTTTCCCAA TGGGGGCATG ACTTTCGTCC TGACTACTTG CGTCTGGGTG 
     1561 CCCTGCGCTC CCGCCTGGGA CATGCCCCTT GTGTGGCTCT GACCGCCACA GCCACCCCAC 
     1621 AGGTCCAAGA GGACGTGTTT GCTGCCCTGC ACCTGAAGAA ACCAGTTGCC ATCTTCAAGA 
     1681 CTCCCTGCTT CCGGGCCAAC CTCTTCTATG ATGTGCAATT CAAGGAACTG ATTTCTGATC 
     1741 CCTATGGGAA CCTGAAGGAC TTCTGCCTTA AGGCTCTTGG ACAGGAGGCT GATAAAGGGT 
     1801 TATCTGGCTG CGGCATTGTG TACTGCAGGA CTAGAGAGGC TTGTGAACAG CTGGCCATAG 
     1861 AGCTCAGCTG CAGGGGTGTG AACGCCAAGG CTTACCATGC AGGGCTGAAG GCCTCTGAAA 
     1921 GAACGCTGGT GCAGAACGAC TGGATGGAGG AGAAGGTCCC TGTAATTGTT GCAACCATTA 
     1981 GTTTTGGGAT GGGAGTGGAT AAAGCCAATG TCAGGTTTGT CGCCCATTGG AATATTGCCA 
     2041 AGTCTATGGC TGGGTACTAC CAGGAGTCTG GCCGGGCTGG CAGGGATGGG AAGCCTTCCT 
     2101 GGTGCCGTCT CTATTACTCC AGGAATGACC GGGACCAAGT CAGCTTCCTG ATCAGGAAGG 
     2161 AAGTAGCAAA ACTCCAGGAA AAGAGAGGAA ACAAAGCATC TGATAAAGCC ACTATCATGG 
     2221 CCTTTGATGC CCTGGTGACC TTCTGTGAAG AACTGGGGTG CCGCCATGCC GCCATTGCCA 
     2281 AGTACTTCGG GGATGCGCTG CCTGCCTGCG CCAAAGGCTG CGACCACTGC CAGAACCCCA 
     2341 CGGCCGTGCG GAGGCGGCTG GAGGCCTTGG AGCGCAGCAG CAGCTGGAGC AAGACCTGCA 
     2401 TCGGGCCCTC CCAGGGGAAC GGCTTTGACC CCGAGCTGTA TGAGGGAGGC CGCAAGGGCT 
     2461 ACGGGGACTT CAGCAGGTAT GACGAAGGTT CTGGAGGCAG CGGGGATGAA GGCAGAGATG 
     2521 AGGCCCACAA GCGGGAGTGG AACCTCTTCT ATCAGAAGCA GATGCAGCTG CGCAAGGGCA 
     2581 AAGACCCCAA GATAGAAGAA TTTGTACCCC CAGATGAGAA CTGTCCCCTG AAAGAGGCTT 
     2641 CTAGCAGGAG GATCCCCAGG CTGACTGTGA AGGCACGGGA GCACTGCCTG CGGCTTCTGG 
     2701 AGGAGGCGCT GAGCAGCAAC CGCCAGTCAA CACGTACCGC TGATGAAGCT GACCTCCGGG 
     2761 CCAAGGCCGT GGAGCTGGAA CATGAGACAT TCCGGAACGC CAAGGTGGCC AACCTCTACA 
     2821 AGGCCAGCGT GCTGAAGAAG GTGGCCGATA TCCACAGAGC CTCCAAGGAT GGGCAGCCCT 
     2881 ATGACATGGG AGGCAGTGCC AAGAGCTGCA GTGCCCAAGC TGAGCCCCCG GAGCCCAATG 
     2941 AGTATGACAT TCCACCAGCC TCCCATGTGT ACTCGCTCAA ACCCAAGCGG GTGGGAGCTG 
     3001 GTTTCCCCAA AGGCTCCTGC CCGTTCCAGA CGGCCACGGA ACTGATGGAG ACAACTCGGA 
     3061 TCAGGGAGCA AGCCCCCCAG CCCGAGCGGG GAGGCGAGCA CGAGCCCCCG AGCCGGCCCT 
     3121 GTGGCCTCCT GGATGAGGAT GGGAGTGAGC CCCTCCCTGG GCCCAGAGGG GAGGTCCCTG 
     3181 GAGGCAGCGC TCACTATGGG GGGCCCTCCC CTGAGAAGAA GGCAAAAAGT TCCTCTGGGG 
     3241 GCAGCTCCCT TGCCAAGGGC CGGGCTAGCA AGAAACAGCA GCTCCTAGCC ACAGCGGCCC 
     3301 ACAAGGATTC TCAGAGCATC GCCCGCTTCT TCTGCCGAAG GGTGGAAAGC CCAGCTCTGC 
     3361 TGGCATCAGC CCCAGAGGCA GAAGGTGCCT GCCCCTCCTG TGAGGGGGTT CAGGGACCCC 
     3421 CGATGGCCCC AGAGAAGTAC ACAGGGGAGG AAGATGGAGC CGGGGGACAT TCGCCTGCCC 
     3481 CTCCCCAGAC TGAGGAGTGC CTCAGGGAGA GGCCAAGCAC CTGCCCGCCC AGAGACCAGG 
     3541 GCACCCCTGA AGTCCAGCCC ACCCCTGCAA AGGACACATG GAAGGGCAAG CGGCCTCGAT 
     3601 CCCAGCAGGA GAACCCAGAG AGCCAGCCTC AGAAGAGGCC ACGCCCCTCA GCCAAGCCCT 
     3661 CCGTCGTAGC TGAGGTCAAG GGCAGCGTCT CGGCCAGCGA ACAGGGCACC TTGAATCCCA 
     3721 CGGCTCAAGA CCCCTTCCAG CTCTCCGCTC CTGGCGTCTC CTTGAAGGAG GCTGCAAATG 
     3781 TTGTGGTCAA GTGCCTCACC CCTTTCTACA AGGAGGGCAA GTTTGCTTCC AAGGAGTTGT 
     3841 TTAAAGGCTT TGCCCGCCAC CTCTCACACT TGCTGACTCA GAAGACCTCT CCTGGAAGGA 
     3901 GCGTGAAAGA AGAGGCCCAG AACCTCATCA GGCACTTCTT CCATGGCCGG GCCCGGTGCG 
     3961 AGAGCGAAGC TGACTGGCAT GGCCTGTGTG GCCCCCAGAG AACGCGTACG CGGCCGCTCG 
     4021 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4081 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4141 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4201 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4261 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4321 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4381 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4441 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4501 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4561 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4621 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4681 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4741 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4801 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4861 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     4921 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     4981 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5041 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5101 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5161 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5221 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5281 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5341 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5401 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5461 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5521 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5581 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5641 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5701 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5761 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5821 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     5881 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     5941 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6001 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6061 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6121 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6181 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6241 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6301 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6361 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6421 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6481 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6541 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6601 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6661 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6721 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6781 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6841 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     6901 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     6961 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7021 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7081 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7141 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7201 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7261 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7321 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7381 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7441 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7501 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7561 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7621 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7681 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7741 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7801 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//