LOCUS       RC209418                7122 bp    DNA     circular  SYN 
DEFINITION  OSBPL11 (Myc-DDK-tagged)-Human oxysterol binding protein-like 11 (OSBPL11)
ACCESSION   NM_022776
VERSION     NM_022776.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3279..3370 
                     /label="Myc-DDK"  
     misc_feature    3400..3985 
                     /label="PolyA signal"  
     misc_feature    3457..3476 
                     /label="Restriction Enzyme"  
     misc_feature    4261..4933 
                     /label="ColE1"  
     misc_feature    5363..6157 
                     /label="Kan/Neo"  
     misc_feature    6192..6494 
                     /label="SV40 ori"  
     misc_feature    6580..7057 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3261..3267 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3261 
                     /label="OSBPL11"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGGGGGGT GAACCAGTGT CCACAATGAA AGTCTCGGAG AGCGAAGGAA 
     1081 AGCTGGAGGG CCAGGCCACA GCGGTGACCC CGAACAAGAA CAGCAGCTGT GGAGGTGGAA 
     1141 TCAGTAGCAG CAGCAGCAGC CGCGGTGGCA GTGCAAAAGG CTGGCAGTAC AGTGATCACA 
     1201 TGGAAAATGT GTATGGCTAT TTAATGAAGT ATACCAACCT TGTCACTGGG TGGCAGTACA 
     1261 GGTTTTTTGT TTTAAACAAT GAAGCTGGGC TGTTGGAGTA CTTTGTGAAT GAACAGTCTA 
     1321 GAAATCAGAA ACCTAGAGGA ACTTTGCAGC TTGCAGGAGC TGTAATATCA CCCAGTGATG 
     1381 AGGATTCTCA CACCTTCACT GTAAACGCTG CCAGTGGGGA ACAATATAAA CTCAGAGCTA 
     1441 CAGATGCAAA AGAGCGACAG CACTGGGTTA GCAGACTTCA GATATGTACA CAGCATCATA 
     1501 CTGAAGCTAT TGGAAAGAAT AATCCTCCTC TGAAGTCACG GAGCTTCTCA CTTGCATCTA 
     1561 GTAGTAATTC TCCTATATCG CAGAGGAGAC CAAGTCAAAA TGCCATTTCT TTTTTTAATG 
     1621 TTGGACATTC CAAACTGCAA TCACTGAGCA AAAGAACTAA TTTACCTCCA GACCATCTTG 
     1681 TGGAAGTCAG AGAAATGATG TCTCATGCTG AAGGACAACA AAGAGACTTA ATTAGACGAA 
     1741 TTGAATGCCT TCCTACTTCT GGCCATCTTA GTTCCTTGGA CCAGGATCTC TTAATGCTCA 
     1801 AAGCTACTTC CATGGCAACT ATGAACTGCT TAAATGACTG CTTTCATATT CTCCAGTTAC 
     1861 AGCATGCATC ACATCAGAAG GGCTCATTGC CTTCAGGAAC GACAATCGAG TGGTTAGAAC 
     1921 CAAAGATATC TTTATCAAAC CACTATAAAA ATGGAGCTGA CCAGCCCTTT GCAACTGATC 
     1981 AGAGTAAGCC GGTGGCAGTC CCAGAAGAGC AGCCTGTTGC AGAATCTGGA CTATTAGCGA 
     2041 GGGAGCCTGA AGAAATAAAT GCAGATGATG AGATAGAGGA TACATGTGAC CACAAAGAGG 
     2101 ATGACCTGGG AGCTGTAGAA GAACAACGTA GTGTCATCCT ACATCTCTTG TCACAGCTTA 
     2161 AGCTGGGCAT GGATTTAACA AGAGTGGTGC TTCCTACATT TATCCTAGAG AAGCGTTCCT 
     2221 TGCTGGAAAT GTATGCAGAC TTTATGTCTC ATCCAGACCT ATTTATAGCC ATCACTAATG 
     2281 GAGCCACAGC TGAGGACAGA ATGATTCGCT TTGTTGAGTA CTACCTTACC TCATTTCATG 
     2341 AAGGCCGTAA GGGAGCCATT GCTAAAAAAC CATACAATCC TATCATTGGA GAAACATTTC 
     2401 ACTGTTCCTG GAAGATGCCA AAAAGCGAGG TAGCATCCAG TGTTTTTAGC AGTTCTTCCA 
     2461 CCCAGGGAGT CACAAATCAT GCTCCTTTAT CGGGGGAGTC TTTGACCCAG GTGGGATCAG 
     2521 ACTGTTACAC AGTCAGATTT GTTGCTGAGC AGGTTTCTCA TCATCCTCCA GTCTCAGGAT 
     2581 TTTATGCAGA ATGTACAGAG AGGAAGATGT GTGTAAATGC GCATGTCTGG ACTAAGAGCA 
     2641 AGTTCTTAGG CATGTCAATA GGCGTGACAA TGGTTGGAGA AGGTATCCTT AGTCTGTTGG 
     2701 AGCATGGAGA AGAGTACACA TTTTCTCTAC CCTGTGCATA TGCTCGGTCA ATTTTGACTG 
     2761 TTCCTTGGGT AGAACTGGGT GGCAAAGTCA GTGTCAACTG TGCAAAAACT GGATATTCAG 
     2821 CCAGCATCAC TTTTCATACC AAGCCATTTT ATGGTGGCAA ACTGCATCGG GTTACAGCTG 
     2881 AAGTAAAGCA CAACATCACC AACACTGTGG TATGCAGAGT GCAAGGGGAA TGGAATAGTG 
     2941 TTCTTGAGTT CACATATAGC AATGGAGAGA CAAAGTATGT GGACTTGACT AAATTGGCAG 
     3001 TGACGAAGAA AAGAGTGAGA CCTCTGGAGA AGCAGGATCC ATTTGAATCC AGGCGATTGT 
     3061 GGAAAAATGT GACAGACTCG CTGAGAGAAT CTGAAATTGA TAAGGCCACA GAGCATAAGC 
     3121 ATACCCTGGA AGAACGTCAG AGGACTGAAG AAAGGCATCG TACTGAAACA GGCACACCTT 
     3181 GGAAAACCAA ATATTTTATT AAAGAGGGAG ATGGCTGGGT TTATCATAAA CCACTTTGGA 
     3241 AAATAATTCC AACAACACAA CCAGCAGAGA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     3301 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     3361 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     3421 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     3481 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     3541 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     3601 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     3661 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     3721 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     3781 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     3841 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     3901 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     3961 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4021 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     4081 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     4141 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     4201 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     4261 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     4321 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     4381 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     4441 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     4501 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     4561 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     4621 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     4681 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     4741 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     4801 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     4861 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     4921 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     4981 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     5041 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     5101 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     5161 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     5221 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     5281 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     5341 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     5401 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     5461 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     5521 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     5581 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     5641 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     5701 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     5761 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     5821 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     5881 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     5941 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6001 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     6061 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     6121 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     6181 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     6241 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     6301 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     6361 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6421 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6481 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     6541 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     6601 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     6661 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     6721 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     6781 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     6841 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     6901 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     6961 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7021 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     7081 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//