LOCUS       RC209244                8499 bp    DNA     circular  SYN 
DEFINITION  GLG1 (Myc-DDK-tagged)-Human golgi glycoprotein 1 (GLG1), transcript variant 1
ACCESSION   NM_012201
VERSION     NM_012201.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4656..4747 
                     /label="Myc-DDK"  
     misc_feature    4777..5362 
                     /label="PolyA signal"  
     misc_feature    4834..4853 
                     /label="Restriction Enzyme"  
     misc_feature    5638..6310 
                     /label="ColE1"  
     misc_feature    6740..7534 
                     /label="Kan/Neo"  
     misc_feature    7569..7871 
                     /label="SV40 ori"  
     misc_feature    7957..8434 
                     /label="f1 ori"  
     misc_feature    1029..1037 
                     /label="Restriction Enzyme"  
     misc_feature    4638..4644 
                     /label="Restriction Enzyme"  
     misc_feature    1029..4638 
                     /label="GLG1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCGG CGCGCCCATG GCGGCGTGTG GACGTGTACG GAGGATGTTC CGCTTGTCGG 
     1081 CGGCGCTGCA TCTGCTGCTG CTATTCGCGG CCGGGGCCGA GAAACTCCCC GGCCAGGGCG 
     1141 TCCACAGCCA GGGCCAGGGT CCCGGGGCCA ACTTTGTGTC CTTCGTAGGG CAGGCCGGAG 
     1201 GCGGCGGCCC GGCGGGTCAG CAGCTGCCCC AGCTGCCTCA GTCATCGCAG CTTCAGCAGC 
     1261 AACAGCAGCA GCAGCAACAG CAACAGCAGC CTCAGCCGCC GCAGCCGCCT TTCCCGGCGG 
     1321 GTGGGCCTCC GGCCCGGCGG GGAGGAGCGG GGGCTGGTGG GGGCTGGAAG CTGGCGGAGG 
     1381 AAGAGTCCTG CAGGGAGGAC GTGACCCGCG TGTGCCCTAA GCACACCTGG AGCAACAACC 
     1441 TGGCGGTGCT CGAGTGCCTG CAGGATGTGA GGGAGCCTGA AAATGAAATT TCTTCAGACT 
     1501 GCAATCATTT GTTGTGGAAT TATAAGCTGA ACCTAACTAC AGATCCCAAA TTTGAATCTG 
     1561 TGGCCAGAGA GGTTTGCAAA TCTACTATAA CAGAGATTAA AGAATGTGCT GATGAACCGG 
     1621 TTGGAAAAGG TTACATGGTT TCCTGCTTGG TGGATCACCG AGGCAACATC ACTGAGTATC 
     1681 AGTGTCACCA GTACATTACC AAGATGACGG CCATCATTTT TAGTGATTAC CGTTTAATCT 
     1741 GTGGCTTCAT GGATGACTGC AAAAATGACA TCAACATTCT GAAATGTGGC AGTATTCGGC 
     1801 TTGGAGAAAA GGATGCACAT TCACAAGGTG AGGTGGTATC ATGCTTGGAG AAAGGCCTGG 
     1861 TGAAAGAAGC AGAAGAAAGA GAACCCAAGA TTCAAGTTTC TGAACTCTGC AAGAAAGCCA 
     1921 TTCTCCGGGT GGCTGAGCTG TCATCGGATG ACTTTCACTT AGACCGGCAT TTATATTTTG 
     1981 CTTGCCGAGA TGATCGGGAG CGTTTTTGTG AAAATACACA AGCTGGTGAG GGCAGAGTGT 
     2041 ATAAGTGCCT CTTTAACCAT AAATTTGAAG AATCCATGAG TGAAAAGTGT CGAGAAGCAC 
     2101 TTACAACCCG CCAAAAGCTG ATTGCCCAGG ATTATAAAGT CAGTTATTCA TTGGCCAAAT 
     2161 CCTGTAAAAG TGACTTGAAG AAATACCGGT GCAATGTGGA AAACCTTCCG CGATCGCGTG 
     2221 AAGCCAGGCT CTCCTACTTG TTAATGTGCC TGGAGTCAGC TGTACACAGA GGGCGACAAG 
     2281 TCAGCAGTGA GTGCCAGGGG GAGATGCTGG ATTACCGACG CATGTTGATG GAAGACTTTT 
     2341 CTCTGAGCCC TGAGATCATC CTAAGCTGTC GGGGGGAGAT TGAACACCAT TGTTCCGGAT 
     2401 TACATCGAAA AGGGCGGACC CTACACTGTC TGATGAAAGT AGTTCGAGGG GAGAAGGGGA 
     2461 ACCTTGGAAT GAACTGCCAG CAGGCGCTTC AAACACTGAT TCAGGAGACT GACCCTGGTG 
     2521 CAGATTACCG CATTGATCGA GCTTTGAATG AAGCTTGTGA ATCTGTAATC CAGACAGCCT 
     2581 GCAAACATAT AAGATCTGGA GACCCAATGA TCTTGTCGTG CCTGATGGAA CATTTATACA 
     2641 CAGAGAAGAT GGTAGAAGAC TGTGAACACC GTCTCTTAGA GCTGCAGTAT TTCATCTCCC 
     2701 GGGATTGGAA GCTGGACCCT GTCCTGTACC GCAAGTGCCA GGGAGACGCT TCTCGTCTTT 
     2761 GCCACACCCA CGGTTGGAAT GAGACCAGTG AATTTATGCC TCAGGGAGCT GTGTTCTCTT 
     2821 GTTTATACAG ACACGCCTAC CGCACTGAGG AACAGGGAAG GAGGCTCTCA CGGGAGTGCC 
     2881 GAGCTGAAGT CCAAAGGATC CTACACCAGC GTGCCATGGA TGTCAAGCTG GATCCTGCCC 
     2941 TCCAGGATAA GTGCCTGATT GATCTGGGAA AATGGTGCAG TGAGAAAACA GAGACTGGAC 
     3001 AGGAGCTGGA GTGCCTTCAG GACCATCTGG ATGACTTGGT GGTGGAGTGT AGAGATATAG 
     3061 TTGGCAACCT CACTGAGTTA GAATCAGAGG ATATTCAAAT AGAAGCCTTG CTGATGAGAG 
     3121 CCTGTGAGCC CATAATTCAG AACTTCTGCC ACGATGTGGC AGATAACCAG ATAGACTCTG 
     3181 GGGACCTGAT GGAGTGTCTG ATACAGAACA AACACCAGAA GGACATGAAC GAGAAGTGTG 
     3241 CCATCGGAGT TACCCACTTC CAGCTGGTGC AGATGAAGGA TTTTCGGTTT TCTTACAAGT 
     3301 TTAAAATGGC CTGCAAGGAG GACGTGTTGA AGCTTTGCCC AAACATAAAA AAGAAGGTGG 
     3361 ACGTGGTGAT CTGCCTGAGC ACGACCGTGC GCAATGACAC TCTGCAGGAA GCCAAGGAGC 
     3421 ACAGGGTGTC CCTGAAGTGC CGCAGGCAGC TCCGTGTGGA GGAGCTGGAG ATGACGGAGG 
     3481 ACATCCGCTT GGAGCCAGAT CTATACGAAG CCTGCAAGAG TGACATCAAA AACTTCTGTT 
     3541 CCGCTGTGCA ATATGGCAAC GCTCAGATTA TCGAATGTCT GAAAGAAAAC AAGAAGCAGC 
     3601 TAAGCACCCG CTGCCACCAA AAAGTATTTA AGCTGCAGGA GACAGAGATG ATGGACCCAG 
     3661 AGCTAGACTA CACCCTCATG AGGGTCTGTA AGCAGATGAT AAAGAGGTTC TGTCCGGAAG 
     3721 CAGATTCTAA AACCATGTTG CAGTGCTTGA AGCAAAATAA AAACAGTGAA TTGATGGATC 
     3781 CCAAATGCAA ACAGATGATA ACCAAGCGCC AGATCACCCA GAACACAGAT TACCGCTTAA 
     3841 ACCCCATGTT AAGAAAAGCC TGTAAAGCTG ACATTCCTAA ATTCTGTCAC GGTATCCTGA 
     3901 CTAAGGCCAA GGATGATTCA GAATTAGAAG GACAAGTCAT CTCTTGCCTG AAGCTGAGAT 
     3961 ATGCTGACCA GCGCCTGTCT TCAGACTGTG AAGACCAGAT CCGAATCATT ATCCAGGAGT 
     4021 CCGCCCTGGA CTACCGCCTG GATCCTCAGC TCCAGCTGCA CTGCTCAGAC GAGATCTCCA 
     4081 GTCTATGTGC TGAAGAAGCA GCAGCCCAAG AGCAGACAGG TCAGGTGGAG GAGTGCCTCA 
     4141 AGGTCAACCT GCTCAAGATC AAAACAGAAT TGTGTAAAAA GGAAGTGCTA AACATGCTGA 
     4201 AGGAAAGCAA AGCAGACATC TTTGTTGACC CGGTACTTCA TACTGCTTGT GCCCTGGACA 
     4261 TTAAACACCA CTGCGCAGCC ATCACCCCTG GCCGCGGGCG TCAAATGTCC TGTCTCATGG 
     4321 AAGCACTGGA GGATAAGCGG GTGAGGTTAC AGCCCGAGTG CAAAAAGCGC CTCAATGACC 
     4381 GGATTGAGAT GTGGAGTTAC GCAGCAAAGG TGGCCCCAGC AGATGGCTTC TCTGATCTTG 
     4441 CCATGCAAGT AATGACGTCT CCATCTAAGA ACTACATTCT CTCTGTGATC AGTGGGAGCA 
     4501 TCTGTATATT GTTCCTGATT GGCCTGATGT GTGGACGGAT CACCAAGCGA GTGACACGAG 
     4561 AGCTCAAGGA CAGGCTACAA TACAGGTCAG AGACAATGGC TTATAAAGGT TTAGTGTGGT 
     4621 CTCAGGATGT GACAGGCAGT CCAGCCACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4681 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4741 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4801 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4861 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4921 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4981 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     5041 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     5101 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5161 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5221 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5281 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5341 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5401 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5461 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5521 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5581 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5641 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5701 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5761 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5821 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5881 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5941 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     6001 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     6061 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6121 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6181 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6241 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6301 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6361 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6421 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6481 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6541 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6601 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6661 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6721 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6781 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6841 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6901 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6961 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     7021 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     7081 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7141 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7201 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7261 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7321 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7381 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7441 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7501 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7561 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7621 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7681 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7741 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7801 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7861 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7921 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7981 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     8041 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     8101 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     8161 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8221 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8281 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8341 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8401 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8461 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//