LOCUS RC209228 8490 bp DNA circular SYN DEFINITION NOS3 (Myc-DDK-tagged)-Human nitric oxide synthase 3 (endothelial cell) (NOS3), transcript variant 1 ACCESSION NM_000603 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 4647..4738 /label="Myc-DDK" misc_feature 4768..5353 /label="PolyA signal" misc_feature 4825..4844 /label="Restriction Enzyme" misc_feature 5629..6301 /label="ColE1" misc_feature 6731..7525 /label="Kan/Neo" misc_feature 7560..7862 /label="SV40 ori" misc_feature 7948..8425 /label="f1 ori" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 4629..4635 /label="Restriction Enzyme" misc_feature 1020..4629 /label="NOS3" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GGGCAACTTG AAGAGCGTGG CCCAGGAGCC TGGGCCACCC TGCGGCCTGG 1081 GGCTGGGGCT GGGCCTTGGG CTGTGCGGCA AGCAGGGCCC AGCCACCCCG GCCCCTGAGC 1141 CCAGCCGGGC CCCAGCATCC CTACTCCCAC CAGCGCCAGA ACACAGCCCC CCGAGCTCCC 1201 CGCTAACCCA GCCCCCAGAG GGGCCCAAGT TCCCTCGTGT GAAGAACTGG GAGGTGGGGA 1261 GCATCACCTA TGACACCCTC AGCGCCCAGG CGCAGCAGGA TGGGCCCTGC ACCCCAAGAC 1321 GCTGCCTGGG CTCCCTGGTA TTTCCACGGA AACTACAGGG CCGGCCCTCC CCCGGCCCCC 1381 CGGCCCCTGA GCAGCTGCTG AGTCAGGCCC GGGACTTCAT CAACCAGTAC TACAGCTCCA 1441 TTAAGAGGAG CGGCTCCCAG GCCCACGAAC AGCGGCTTCA AGAGGTGGAA GCCGAGGTGG 1501 CAGCCACAGG CACCTACCAG CTTAGGGAGA GCGAGCTGGT GTTCGGGGCT AAGCAGGCCT 1561 GGCGCAACGC TCCCCGCTGC GTGGGCCGGA TCCAGTGGGG GAAGCTGCAG GTGTTCGATG 1621 CCCGGGACTG CAGGTCTGCA CAGGAAATGT TCACCTACAT CTGCAACCAC ATCAAGTATG 1681 CCACCAACCG GGGCAACCTT CGCTCGGCCA TCACAGTGTT CCCGCAGCGC TGCCCTGGCC 1741 GAGGAGACTT CCGAATCTGG AACAGCCAGC TGGTGCGCTA CGCGGGCTAC CGGCAGCAGG 1801 ACGGCTCTGT GCGGGGGGAC CCAGCCAACG TGGAGATCAC CGAGCTCTGC ATTCAGCACG 1861 GCTGGACCCC AGGAAACGGT CGCTTCGACG TGCTGCCCCT GCTGCTGCAG GCCCCAGATG 1921 AGCCCCCAGA ACTCTTCCTT CTGCCCCCCG AGCTGGTCCT TGAGGTGCCC CTGGAGCACC 1981 CCACGCTGGA GTGGTTTGCA GCCCTGGGCC TGCGCTGGTA CGCCCTCCCG GCAGTGTCCA 2041 ACATGCTGCT GGAAATTGGG GGCCTGGAGT TCCCCGCAGC CCCCTTCAGT GGCTGGTACA 2101 TGAGCACTGA GATCGGCACG AGGAACCTGT GTGACCCTCA CCGCTACAAC ATCCTGGAGG 2161 ATGTGGCTGT CTGCATGGAC CTGGATACCC GGACCACCTC GTCCCTGTGG AAAGACAAGG 2221 CAGCAGTGGA AATCAACGTG GCCGTGCTGC ACAGTTACCA GCTAGCCAAA GTCACCATCG 2281 TGGACCACCA CGCCGCCACG GCCTCTTTCA TGAAGCACCT GGAGAATGAG CAGAAGGCCA 2341 GGGGGGGCTG CCCTGCAGAC TGGGCCTGGA TCGTGCCCCC CATCTCGGGC AGCCTCACTC 2401 CTGTTTTCCA TCAGGAGATG GTCAACTATT TCCTGTCCCC GGCCTTCCGC TACCAGCCAG 2461 ACCCCTGGAA GGGGAGTGCC GCCAAGGGCA CCGGCATCAC CAGGAAGAAG ACCTTTAAAG 2521 AAGTGGCCAA CGCCGTGAAG ATCTCCGCCT CGCTCATGGG CACGGTGATG GCGAAGCGAG 2581 TGAAGGCGAC AATCCTGTAT GGCTCCGAGA CCGGCCGGGC CCAGAGCTAC GCACAGCAGC 2641 TGGGGAGACT CTTCCGGAAG GCTTTTGATC CCCGGGTCCT GTGTATGGAT GAGTATGACG 2701 TGGTGTCCCT CGAACACGAG ACGCTGGTGC TGGTGGTAAC CAGCACATTT GGGAATGGGG 2761 ATCCCCCGGA GAATGGAGAG AGCTTTGCAG CTGCCCTGAT GGAGATGTCC GGCCCCTACA 2821 ACAGCTCCCC TCGGCCGGAA CAGCACAAGA GTTATAAGAT CCGCTTCAAC AGCATCTCCT 2881 GCTCAGACCC ACTGGTGTCC TCTTGGCGGC GGAAGAGGAA GGAGTCCAGT AACACAGACA 2941 GTGCAGGGGC CCTGGGCACC CTCAGGTTCT GTGTGTTCGG GCTCGGCTCC CGGGCATACC 3001 CCCACTTCTG CGCCTTTGCT CGTGCGGTGG ACACACGGCT GGAGGAACTG GGCGGGGAGC 3061 GGCTGCTGCA GCTGGGCCAG GGCGACGAGC TGTGCGGCCA GGAGGAGGCC TTCCGAGGCT 3121 GGGCCCAGGC TGCCTTCCAG GCCGCCTGTG AGACCTTCTG TGTGGGAGAG GATGCCAAGG 3181 CCGCCGCCCG AGACATCTTC AGCCCCAAAC GGAGCTGGAA GCGCCAGAGG TACCGGCTGA 3241 GCGCCCAGGC CGAGGGCCTG CAGTTGCTGC CAGGTCTGAT CCACGTGCAC AGGCGGAAGA 3301 TGTTCCAGGC TACAATCCGC TCAGTGGAAA ACCTGCAAAG CAGCAAGTCC ACGAGGGCCA 3361 CCATCCTGGT GCGCCTGGAC ACCGGAGGCC AGGAGGGGCT GCAGTACCAG CCGGGGGACC 3421 ACATAGGTGT CTGCCCGCCC AACCGGCCCG GCCTTGTGGA GGCGCTGCTG AGCCGCGTGG 3481 AGGACCCGCC GGCGCCCACT GAGCCCGTGG CAGTAGAGCA GCTGGAGAAG GGCAGCCCTG 3541 GTGGCCCTCC CCCCGGCTGG GTGCGGGACC CCCGGCTGCC CCCGTGCACG CTGCGCCAGG 3601 CTCTCACCTT CTTCCTGGAC ATCACCTCCC CACCCAGCCC TCAGCTCTTG CGGCTGCTCA 3661 GCACCTTGGC AGAAGAGCCC AGGGAACAGC AGGAGCTGGA GGCCCTCAGC CAGGATCCCC 3721 GACGCTACGA GGAGTGGAAG TGGTTCCGCT GCCCCACGCT GCTGGAGGTG CTGGAGCAGT 3781 TCCCGTCGGT GGCGCTGCCT GCCCCACTGC TCCTCACCCA GCTGCCTCTG CTCCAGCCCC 3841 GGTACTACTC AGTCAGCTCG GCACCCAGCA CCCACCCAGG AGAGATCCAC CTCACTGTAG 3901 CTGTGCTGGC ATACAGGACT CAGGATGGGC TGGGCCCCCT GCACTATGGA GTCTGCTCCA 3961 CGTGGCTAAG CCAGCTCAAG CCCGGAGACC CTGTGCCCTG CTTCATCCGG GGGGCTCCCT 4021 CCTTCCGGCT GCCACCCGAT CCCAGCTTGC CCTGCATCCT GGTGGGTCCA GGCACTGGCA 4081 TTGCCCCCTT CCGGGGATTC TGGCAGGAGC GGCTGCATGA CATTGAGAGC AAAGGGCTGC 4141 AGCCCACTCC CATGACTTTG GTGTTCGGCT GCCGATGCTC CCAACTTGAC CATCTCTACC 4201 GCGACGAGGT GCAGAACGCC CAGCAGCGCG GGGTGTTTGG CCGAGTCCTC ACCGCCTTCT 4261 CCCGGGAACC TGACAACCCC AAGACCTACG TGCAGGACAT CCTGAGGACG GAGCTGGCTG 4321 CGGAGGTGCA CCGCGTGCTG TGCCTCGAGC GGGGCCACAT GTTTGTCTGC GGCGATGTTA 4381 CCATGGCAAC CAACGTCCTG CAGACCGTGC AGCGCATCCT GGCGACGGAG GGCGACATGG 4441 AGCTGGACGA GGCCGGCGAC GTCATCGGCG TGCTGCGGGA TCAGCAACGC TACCACGAAG 4501 ACATTTTCGG GCTCACGCTG CGCACCCAGG AGGTGACAAG CCGCATACGC ACCCAGAGCT 4561 TTTCCTTGCA GGAGCGTCAG TTGCGGGGCG CAGTGCCCTG GGCGTTCGAC CCTCCCGGCT 4621 CAGACACCAA CAGCCCCACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 4681 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 4741 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 4801 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 4861 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 4921 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 4981 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 5041 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 5101 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 5161 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 5221 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 5281 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 5341 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 5401 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 5461 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 5521 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 5581 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 5641 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 5701 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 5761 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 5821 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 5881 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 5941 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 6001 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 6061 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 6121 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 6181 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 6241 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 6301 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 6361 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 6421 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 6481 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 6541 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 6601 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 6661 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 6721 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 6781 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 6841 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 6901 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 6961 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 7021 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 7081 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 7141 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 7201 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 7261 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 7321 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 7381 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 7441 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 7501 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 7561 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 7621 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 7681 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 7741 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 7801 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 7861 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 7921 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 7981 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 8041 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 8101 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 8161 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 8221 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 8281 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 8341 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 8401 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 8461 CTGATTTAAC AAAAATTTAA CGCGAATTTT //