LOCUS       RC209193L1                8766 bp    DNA     circular  SYN 
DEFINITION  CASD1 (Myc-DDK-tagged)-Human CAS1 domain containing 1 (CASD1)
ACCESSION   NM_022900
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7057..7237 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    7965..8624 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7396..7964 
                     /label="ColE1"  
     misc_feature    5932..5952 
                     /label="LR50 primer"  
     misc_feature    5729..5800 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    5941..6532 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5711..5717 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5711 
                     /label="CASD1"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGCGGCTCTG GCCTACAACC TGGGCAAGCG 
     3361 GGAGATCAAC CACTACTTCA GCGTGAGGAG CGCCAAGGTG CTGGCGCTGG TGGCCGTGCT 
     3421 GCTGCTCGCA GCGTGCCACC TCGCCTCCCG CCGCTACCGA GGCAATGATT CGTGTGAATA 
     3481 CCTTCTCTCA AGTGGCAGAT TTCTTGGAGA GAAAGTTTGG CAACCTCACA GTTGTATGAT 
     3541 GCATAAATAC AAAATCAGTG AAGCAAAGAA CTGCCTTGTA GATAAACATA TTGCATTTAT 
     3601 TGGAGATTCC AGAATTCGTC AATTGTTTTA TTCTTTTGTA AAAATAATTA ATCCCCAATT 
     3661 CAAAGAAGAA GGAAATAAGC ATGAAAACAT TCCTTTTGAA GACAAGACTG CATCAGTTAA 
     3721 AGTGGATTTT CTGTGGCATC CTGAAGTTAA TGGTTCTATG AAACAGTGTA TCAAAGTGTG 
     3781 GACTGAGGAT TCCATTGCAA AGCCACATGT GATTGTAGCA GGAGCTGCCA CATGGTCCAT 
     3841 CAAGATTCAC AATGGTAGCA GTGAAGCGCT TTCTCAATAT AAAATGAACA TCACCTCCAT 
     3901 AGCACCACTT TTAGAAAAAT TGGCAAAGAC TAGTGATGTT TATTGGGTCT TACAAGATCC 
     3961 TGTTTATGAA GATCTATTAA GTGAAAATAG GAAGATGATC ACTAATGAGA AGATAGATGC 
     4021 TTACAATGAA GCTGCAGTCA GTATTTTGAA TAGTAGCACC AGAAATTCTA AATCAAATGT 
     4081 TAAGATGTTC AGTGTTTCCA AATTAATTGC TCAAGAAACC ATCATGGAAT CTTTGGATGG 
     4141 CTTACATCTT CCTGAATCGA GCAGAGAAAC TACTGCAATG ATTCTTATGA ATGTGTATTG 
     4201 CAATAAGATT TTGAAGCCTG TAGATGGGTC CTGTTGTCAA CCTCGGCCTC CTGTTACTCT 
     4261 CATACAGAAG CTAGCTGCTT GTTTTTTCAC TTTATCTATT ATCGGATATT TAATTTTTTA 
     4321 CATAATTCAT CGTAATGCTC ATCGGAAGAA TAAGCCGTGT ACTGATTTGG AAAGTGGAGA 
     4381 GGAAAAGAAA AATATTATCA ATACCCCTGT GTCTTCATTA GAAATACTTT TACAATCTTT 
     4441 CTGCAAACTT GGCCTGATTA TGGCATATTT CTATATGTGT GACAGTGCAA ATCTGTTCAT 
     4501 GAAGGAAAAC AAATTTTATA CACATTCATC TTTCTTTATT CCAATTATCT ACATTTTGGT 
     4561 TTTGGGAGTA TTTTATAATG AAAATACTAA AGAGACTAAA GTATTAAATA GAGAACAAAC 
     4621 AGACGAATGG AAAGGCTGGA TGCAACTTGT GATTTTGATT TATCACATTT CTGGAGCAAG 
     4681 TACATTTTTG CCTGTATACA TGCACATTCG AGTTCTGGTT GCTGCATATT TATTTCAGAC 
     4741 AGGGTATGGG CATTTCTCAT ACTTTTGGAT AAAAGGAGAT TTTGGAATCT ACAGAGTATG 
     4801 TCAGGTTTTA TTTCGTCTCA ATTTCCTGGT AGTGGTGTTA TGTATAGTAA TGGATCGACC 
     4861 TTATCAATTC TATTACTTTG TCCCCTTGGT CACTGTATGG TTCATGGTCA TATATGTTAC 
     4921 TTTAGCACTA TGGCCACAAA TAATCCAAAA AAAAGCAAAC GGAAATTGTT TCTGGCATTT 
     4981 TGGCTTACTG TTGAAACTAG GCTTTTTGCT GTTATTCATA TGTTTTTTGG CATATTCTCA 
     5041 GGGTGCATTT GAGAAGATCT TTTCTCTTTG GCCATTGTCC AAGTGTTTTG AACTGAAAGG 
     5101 GAATGTATAT GAATGGTGGT TCAGATGGAG GTTAGACCGT TATGTAGTTT TCCACGGAAT 
     5161 GCTGTTTGCT TTTATTTATC TGGCCTTGCA GAAGCGTCAA ATACTTTCTG AAGGAAAGGG 
     5221 TGAACCTCTT TTTTCAAACA AAATTTCAAA TTTTCTGTTG TTTATTTCAG TAGTTTCTTT 
     5281 CTTGACCTAT TCCATCTGGG CTAGCAGTTG TAAAAACAAA GCAGAGTGCA ATGAACTCCA 
     5341 TCCGTCTGTT TCTGTGGTAC AGATTTTAGC CTTCATCCTA ATAAGAAACA TCCCTGGATA 
     5401 TGCCCGTTCA GTTTACAGTT CATTTTTTGC TTGGTTTGGA AAAATTTCAT TAGAGCTATT 
     5461 TATTTGCCAG TATCACATAT GGCTGGCAGC GGACACAAGG GGTATCTTGG TACTGATACC 
     5521 TGGAAACCCT ATGCTCAACA TCATTGTCAG CACTTTCATA TTTGTTTGTG TGGCACATGA 
     5581 AATTTCTCAG ATCACTAATG ATCTTGCACA GATTATTATT CCTAAAGATA ACTCATCTCT 
     5641 CTTGAAAAGG TTGGCATGTA TAGCTGCATT TTTTTGTGGA CTCCTCATCT TATCATCCAT 
     5701 TCAAGATAAA TCAAAACATA CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA 
     5761 GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC 
     5821 CGGCCGCGGT CTGTACAAGT AGGATTCGTC GAGGGACCTA ATAACTTCGT ATAGCATACA 
     5881 TTATACGAAG TTATACATGT TTAAGGGTTC CGGTTCCACT AGGTACAATT CGATATCAAG 
     5941 CTTATCGATA ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC 
     6001 TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT 
     6061 GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT GTCTCTTTAT 
     6121 GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA 
     6181 ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC 
     6241 CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG CTGGACAGGG 
     6301 GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAATCATC GTCCTTTCCT 
     6361 TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG CTACGTCCCT 
     6421 TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT 
     6481 CCGCGTCTTC GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC CTCCCCGCAT 
     6541 CGATACCGTC GACCTCGATC GAGACCTAGA AAAACATGGA GCAATCACAA GTAGCAATAC 
     6601 AGCAGCTACC AATGCTGATT GTGCCTGGCT AGAAGCACAA GAGGAGGAGG AGGTGGGTTT 
     6661 TCCAGTCACA CCTCAGGTAC CTTTAAGACC AATGACTTAC AAGGCAGCTG TAGATCTTAG 
     6721 CCACTTTTTA AAAGAAAAGG GGGGACTGGA AGGGCTAATT CACTCCCAAC GAAGACAAGA 
     6781 TATCCTTGAT CTGTGGATCT ACCACACACA AGGCTACTTC CCTGATTGGC AGAACTACAC 
     6841 ACCAGGGCCA GGGATCAGAT ATCCACTGAC CTTTGGATGG TGCTACAAGC TAGTACCAGT 
     6901 TGAGCAAGAG AAGGTAGAAG AAGCCAATGA AGGAGAGAAC ACCCGCTTGT TACACCCTGT 
     6961 GAGCCTGCAT GGGATGGATG ACCCGGAGAG AGAAGTATTA GAGTGGAGGT TTGACAGCCG 
     7021 CCTAGCATTT CATCACATGG CCCGAGAGCT GCATCCGGAC TGTACTGGGT CTCTCTGGTT 
     7081 AGACCAGATC TGAGCCTGGG AGCTCTCTGG CTAACTAGGG AACCCACTGC TTAAGCCTCA 
     7141 ATAAAGCTTG CCTTGAGTGC TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA 
     7201 CTAGAGATCC CTCAGACCCT TTTAGTCAGT GTGGAAAATC TCTAGCAGCA TGTGAGCAAA 
     7261 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     7321 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     7381 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     7441 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     7501 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     7561 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     7621 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     7681 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     7741 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     7801 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     7861 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     7921 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGATTACGCC 
     7981 CCGCCCTGCC ACTCATCGCA GTACTGTTGT AATTCATTAA GCATTCTGCC GACATGGAAG 
     8041 CCATCACAAA CGGCATGATG AACCTGAATC GCCAGCGGCA TCAGCACCTT GTCGCCTTGC 
     8101 GTATAATATT TGCCCATGGT GAAAACGGGG GCGAAGAAGT TGTCCATATT GGCCACGTTT 
     8161 AAATCAAAAC TGGTGAAACT CACCCAGGGA TTGGCTGAGA CGAAAAACAT ATTCTCAATA 
     8221 AACCCTTTAG GGAAATAGGC CAGGTTTTCA CCGTAACACG CCACATCTTG CGAATATATG 
     8281 TGTAGAAACT GCCGGAAATC GTCGTGGTAT TCACTCCAGA GCGATGAAAA CGTTTCAGTT 
     8341 TGCTCATGGA AAACGGTGTA ACAAGGGTGA ACACTATCCC ATATCACCAG CTCACCGTCT 
     8401 TTCATTGCCA TACGGAACTC CGGATGAGCA TTCATCAGGC GGGCAAGAAT GTGAATAAAG 
     8461 GCCGGATAAA ACTTGTGCTT ATTTTTCTTT ACGGTCTTTA AAAAGGCCGT AATATCCAGC 
     8521 TGAACGGTCT GGTTATAGGT ACATTGAGCA ACTGACTGAA ATGCCTCAAA ATGTTCTTTA 
     8581 CGATGCCATT GGGATATATC AACGGTGGTA TATCCAGTGA TTTTTTTCTC CATACTCTTC 
     8641 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     8701 GAATGTATTT AGAAAAATAA ACAAATAGGG GTCCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8761 CCTGAC
//