LOCUS       RC209192                8544 bp    DNA     circular  SYN 
DEFINITION  ADAMTS18 (Myc-DDK-tagged)-Human ADAM metallopeptidase with thrombospondin type 1 motif, 18 (ADAMTS18)
ACCESSION   NM_199355
VERSION     NM_199355.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4701..4792 
                     /label="Myc-DDK"  
     misc_feature    4822..5407 
                     /label="PolyA signal"  
     misc_feature    4879..4898 
                     /label="Restriction Enzyme"  
     misc_feature    5683..6355 
                     /label="ColE1"  
     misc_feature    6785..7579 
                     /label="Kan/Neo"  
     misc_feature    7614..7916 
                     /label="SV40 ori"  
     misc_feature    8002..8479 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4683..4689 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4683 
                     /label="ADAMTS18"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGTGCGCC CTCCTGCTCG CGTGTGCCTT CCCGGCTGCG GGTTCGGGCC 
     1081 CGCCGAGGGG CCTGGCGGGA CTGGGGCGCG TGGCCAAGGC GCTCCAGCTG TGCTGCCTCT 
     1141 GCTGTGCGTC GGTCGCCGCG GCCTTAGCCA GTGACAGCAG CAGCGGCGCC AGCGGATTAA 
     1201 ATGATGATTA CGTCTTTGTC ACGCCAGTAG AAGTAGACTC AGCCGGGTCA TATATTTCAC 
     1261 ACGACATTTT GCACAACGGC AGGAAAAAGC GATCGGCGCA GAATGCCAGA AGCTCCCTGC 
     1321 ACTACCGATT TTCAGCATTT GGACAGGAAC TGCACTTAGA ACTTAAGCCC TCGGCGATTT 
     1381 TGAGCAGTCA CTTTATTGTC CAGGTACTTG GAAAAGATGG TGCTTCAGAG ACTCAGAAAC 
     1441 CCGAGGTGCA GCAATGCTTC TATCAGGGAT TTATCAGAAA TGACAGCTCC TCCTCTGTCG 
     1501 CTGTGTCTAC GTGTGCTGGC TTGTCAGGTT TAATAAGGAC ACGAAAAAAT GAATTCCTCA 
     1561 TCTCGCCATT ACCTCAGCTT CTGGCCCAGG AACACAACCA CAGCTCCCCT GCGGGTCACC 
     1621 ATCCTCACGT ACTGTACAAA AGGACAGCAG AGGAGAAGAT CCAGCGGTAC CGTGGCTACC 
     1681 CCGGCTCTGG CCGGAATTAT CCTGGTTACT CCCCAAGTCA CATTCCCCAT GCATCTCAGA 
     1741 GTCGAGAGAC AGAGTATCAC CATCGAAGGT TGCAAAAGCA GCATTTTTGT GGACGACGCA 
     1801 AGAAATATGC TCCCAAGCCT CCCACAGAGG ACACCTATCT AAGGTTTGAT GAATATGGGA 
     1861 GCTCTGGGCG ACCCAGAAGA TCAGCTGGAA AATCACAAAA GGGCCTCAAT GTGGAAACCC 
     1921 TCGTGGTGGC AGACAAGAAA ATGGTGGAAA AGCATGGCAA GGGAAATGTC ACCACATACA 
     1981 TTCTCACAGT AATGAACATG GTTTCTGGCC TATTTAAAGA TGGGACTATT GGAAGTGACA 
     2041 TAAACGTGGT TGTGGTGAGC CTAATTCTTC TGGAACAAGA ACCTGGAGGA TTATTGATCA 
     2101 ACCATCATGC AGACCAGTCT CTGAATAGTT TTTGTCAATG GCAGTCTGCC CTCATTGGAA 
     2161 AGAATGGCAA GAGACATGAT CATGCCATCT TACTAACAGG ATTTGATATT TGTTCTTGGA 
     2221 AGAATGAACC ATGTGACACT CTAGGGTTTG CCCCCATCAG TGGAATGTGC TCTAAGTACC 
     2281 GAAGTTGTAC CATCAATGAG GACACAGGAC TTGGCCTTGC CTTCACCATC GCTCATGAGT 
     2341 CAGGGCACAA CTTTGGTATG ATTCACGACG GAGAAGGGAA TCCCTGCAGA AAGGCTGAAG 
     2401 GCAATATCAT GTCTCCCACA CTGACCGGAA ACAATGGAGT GTTTTCATGG TCTTCCTGCA 
     2461 GCCGCCAGTA TCTCAAGAAA TTCCTCAGCA CACCTCAGGC GGGGTGTCTA GTGGATGAGC 
     2521 CCAAGCAAGC AGGACAGTAT AAATATCCGG ACAAACTACC AGGACAGATT TATGATGCTG 
     2581 ACACACAGTG TAAATGGCAA TTTGGAGCAA AAGCCAAGTT ATGCAGCCTT GGTTTTGTGA 
     2641 AGGATATTTG CAAATCACTT TGGTGCCACC GAGTAGGCCA CAGGTGTGAG ACCAAGTTTA 
     2701 TGCCCGCAGC AGAAGGGACC GTTTGTGGCT TGAGTATGTG GTGTCGGCAA GGCCAGTGCG 
     2761 TAAAGTTTGG GGAGCTCGGG CCCCGGCCCA TCCACGGCCA GTGGTCCGCC TGGTCGAAGT 
     2821 GGTCAGAATG TTCCCGGACA TGTGGTGGAG GAGTCAAGTT CCAGGAGAGA CACTGCAATA 
     2881 ACCCCAAGCC TCAGTATGGT GGCATATTCT GTCCAGGTTC TAGCCGTATT TATCAGCTGT 
     2941 GCAATATTAA CCCTTGCAAT GAAAATAGCT TGGATTTTCG GGCTCAACAG TGTGCAGAAT 
     3001 ATAACAGCAA ACCTTTCCGT GGATGGTTCT ACCAGTGGAA ACCCTATACA AAAGTGGAAG 
     3061 AGGAAGATCG ATGCAAACTG TACTGCAAGG CTGAGAACTT TGAATTTTTT TTTGCAATGT 
     3121 CCGGCAAAGT GAAAGATGGA ACTCCCTGCT CCCCAAACAA AAATGATGTT TGTATTGACG 
     3181 GGGTTTGTGA ACTAGTGGGA TGTGATCATG AACTAGGCTC TAAAGCAGTT TCAGATGCTT 
     3241 GTGGCGTTTG CAAAGGTGAT AATTCAACTT GCAAGTTTTA TAAAGGCCTG TACCTCAACC 
     3301 AGCATAAAGC AAATGAATAT TATCCGGTGG TCATCATTCC AGCTGGCGCC CGAAGCATCG 
     3361 AAATCCAGGA GCTGCAGGTT TCCTCCAGTT ACCTCGCAGT TCGAAGCCTC AGTCAAAAGT 
     3421 ATTACCTCAC CGGGGGCTGG AGCATCGACT GGCCTGGGGA GTTCCCCTTC GCTGGGACCA 
     3481 CGTTTGAATA CCAGCGCTCT TTCAACCGCC CGGAACGTCT GTACGCGCCA GGGCCCACAA 
     3541 ATGAGACGCT GGTCTTTGAA ATTCTGATGC AAGGCAAAAA TCCAGGGATA GCTTGGAAGT 
     3601 ATGCACTTCC CAAGGTCATG AATGGAACTC CACCAGCCAC AAAAAGACCT GCCTATACCT 
     3661 GGAGTATCGT GCAGTCAGAG TGCTCCGTCT CCTGTGGTGG AGGTTACATA AATGTAAAGG 
     3721 CCATTTGCTT GCGAGATCAA AATACTCAAG TCAATTCCTC ATTCTGCAGT GCAAAAACCA 
     3781 AGCCAGTAAC TGAGCCCAAA ATCTGCAACG CTTTCTCCTG CCCGGCTTAC TGGATGCCAG 
     3841 GTGAATGGAG TACATGCAGC AAGGCCTGTG CTGGAGGCCA GCAGAGCCGA AAGATCCAGT 
     3901 GTGTGCAAAA GAAGCCCTTC CAAAAGGAGG AAGCAGTGTT GCATTCTCTC TGTCCAGTGA 
     3961 GCACACCCAC TCAGGTCCAA GCCTGCAACA GCCATGCCTG CCCTCCACAA TGGAGCCTTG 
     4021 GACCCTGGTC TCAGTGTTCC AAGACCTGTG GACGAGGGGT GAGGAAGCGT GAACTCCTCT 
     4081 GCAAGGGCTC TGCCGCAGAA ACCCTCCCCG AGAGCCAGTG TACCAGTCTC CCCAGACCTG 
     4141 AGCTGCAGGA GGGCTGTGTG CTTGGACGAT GCCCCAAGAA CAGCCGGCTA CAGTGGGTCG 
     4201 CTTCTTCGTG GAGCGAGTGT TCTGCAACCT GTGGTTTGGG TGTGAGGAAG AGGGAGATGA 
     4261 AGTGCAGCGA GAAGGGCTTC CAGGGAAAGC TGATAACTTT CCCAGAGCGA AGATGCCGTA 
     4321 ATATTAAGAA ACCAAATCTG GACTTGGAAG AGACCTGCAA CCGACGGGCT TGCCCAGCCC 
     4381 ATCCAGTGTA CAACATGGTA GCTGGATGGT ATTCATTGCC GTGGCAGCAG TGCACAGTCA 
     4441 CCTGTGGGGG AGGGGTCCAG ACCCGGTCAG TCCACTGTGT TCAGCAAGGC CGGCCTTCCT 
     4501 CAAGTTGTCT GCTCCATCAG AAACCTCCGG TGCTACGAGC CTGTAATACA AACTTCTGTC 
     4561 CAGCTCCTGA AAAGAGAGAG GATCCATCCT GCGTAGATTT CTTCAACTGG TGTCACCTAG 
     4621 TTCCTCAGCA TGGTGTCTGC AACCACAAGT TTTACGGAAA ACAATGCTGC AAGTCATGCA 
     4681 CAAGGAAGAT CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4741 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4801 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4861 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4921 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4981 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5041 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5101 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5161 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5221 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5281 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5341 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5401 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5461 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5521 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5581 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5641 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5701 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5761 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5821 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5881 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5941 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6001 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6061 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6121 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6181 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6241 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6301 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6361 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6421 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6481 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6541 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6601 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6661 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6721 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6781 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6841 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6901 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6961 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7021 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7081 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7141 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7201 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7261 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7321 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7381 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7441 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7501 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7561 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7621 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7681 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7741 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7801 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7861 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7921 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7981 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8041 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8101 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8161 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8221 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8281 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8341 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8401 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8461 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8521 TAACAAAAAT TTAACGCGAA TTTT
//