LOCUS RC209189 8613 bp DNA circular SYN DEFINITION ALPK1 (Myc-DDK-tagged)-Human alpha-kinase 1 (ALPK1), transcript variant 1 ACCESSION NM_025144 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 4770..4861 /label="Myc-DDK" misc_feature 4891..5476 /label="PolyA signal" misc_feature 4948..4967 /label="Restriction Enzyme" misc_feature 5752..6424 /label="ColE1" misc_feature 6854..7648 /label="Kan/Neo" misc_feature 7683..7985 /label="SV40 ori" misc_feature 8071..8548 /label="f1 ori" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 4752..4758 /label="Restriction Enzyme" misc_feature 1020..4752 /label="ALPK1" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GAATAATCAA AAAGTGGTAG CTGTGCTACT GCAAGAGTGC AAGCAAGTGC 1081 TGGATCAGCT CTTGTTGGAA GCGCCAGATG TGTCGGAAGA GGACAAGAGC GAGGACCAGC 1141 GCTGCAGAGC TTTACTCCCC AGCGAGTTAA GGACCCTGAT CCAGGAGGCA AAGGAAATGA 1201 AGTGGCCCTT CGTGCCTGAA AAGTGGCAGT ACAAACAAGC CGTGGGCCCA GAGGACAAAA 1261 CAAACCTGAA GGATGTGATT GGCGCCGGGT TGCAGCAGTT ACTGGCGTCC CTGAGGGCCT 1321 CCATCCTCGC TCGGGACTGT GCGGCTGCGG CGGCTATTGT GTTCTTGGTG GACCGGTTCC 1381 TGTATGGGCT CGACGTCTCT GGAAAACTTC TGCAGGTCGC CAAAGGTCTC CACAAGTTGC 1441 AGCCAGCCAC GCCAATTGCC CCGCAGGTGG TTATTCGCCA AGCCCGAATC TCCGTGAACT 1501 CAGGAAAACT TTTAAAAGCA GAGTATATTC TGAGCAGTCT AATAAGCAAC AATGGAGCAA 1561 CGGGTACCTG GCTGTACAGA AATGAAAGTG ACAAGGTCCT GGTGCAGTCG GTCTGTATAC 1621 AGATCAGAGG GCAGATTCTG CAAAAGCTGG GGATGTGGTA CGAAGCAGCA GAGTTAATAT 1681 GGGCCTCCAT TGTAGGATAT TTGGCACTTC CTCAGCCGGA TAAAAAGGGC CTCTCCACGT 1741 CGCTAGGTAT ACTGGCAGAC ATCTTTGTTT CCATGAGCAA GAACGATTAT GAAAAGTTTA 1801 AAAACAATCC ACAAATTAAT TTGAGCCTGC TGAAGGAGTT TGACCACCAT TTGCTGTCCG 1861 CTGCAGAAGC CTGCAAGCTG GCAGCTGCCT TCAGTGCCTA TACGCCGCTC TTCGTGCTCA 1921 CAGCTGTGAA TATCCGTGGC ACGTGTTTAT TGTCCTACAG TAGTTCAAAT GACTGTCCTC 1981 CAGAATTGAA AAACTTACAT CTGTGTGAAG CCAAAGAGGC CTTTGAGATT GGCCTCCTCA 2041 CCAAGAGAGA TGATGAGCCT GTTACTGGAA AACAGGAGCT TCACAGCTTT GTCAAAGCTG 2101 CTTTCGGTCT CACCACAGTG CACAGAAGGC TCCATGGGGA GACAGGGACG GTCCATGCAG 2161 CAAGTCAGCT CTGTAAGGAA GCAATGGGGA AGCTGTACAA TTTCAGCACT TCCTCCAGAA 2221 GTCAGGACAG AGAAGCTCTG TCTCAAGAAG TTATGTCTGT GATTGCCCAG GTGAAGGAAC 2281 ATTTACAAGT TCAAAGCTTC TCAAATGTAG ATGACAGATC TTATGTTCCC GAGAGTTTCG 2341 AGTGCAGGTT GGATAAACTT ATCTTGCATG GGCAAGGGGA TTTCCAAAAA ATCCTTGACA 2401 CCTATTCACA GCACCATACT TCGGTGTGTG AAGTATTTGA AAGTGATTGT GGAAACAACA 2461 AAAATGAACA GAAAGATGCA AAAACAGGAG TCTGCATCAC TGCTCTAAAA ACAGAAATAA 2521 AAAACATAGA TACTGTGAGT ACTACTCAAG AAAAGCCACA TTGTCAAAGA GACACAGGAA 2581 TATCTTCCTC CCTAATGGGT AAGAATGTTC AGAGGGAACT CAGAAGGGGA GGAAGGAGAA 2641 ACTGGACCCA TTCTGATGCA TTTCGAGTCT CCTTGGATCA AGATGTGGAG ACTGAGACTG 2701 AGCCATCGGA CTACAGCAAT GGTGAGGGAG CTGTTTTCAA CAAGTCTCTG AGTGGCAGCC 2761 AGACTTCCAG TGCTTGGAGC AACTTATCAG GGTTTAGTTC CTCTGCAAGC TGGGAGGAAG 2821 TGAATTATCA CGTTGACGAC AGGTCAGCCA GAAAAGAGCC TGGCAAAGAA CATCTGGTGG 2881 ACACTCAGTG TTCCACTGCC TTGTCTGAGG AGCTAGAGAA TGACAGGGAA GGCAGAGCTA 2941 TGCATTCATT GCATTCACAG CTTCATGATC TCTCTCTTCA GGAACCCAAC AATGACAATT 3001 TGGAGCCTTC TCAAAATCAG CCACAGCAAC AGATGCCCTT GACACCCTTC TCGCCTCATA 3061 ATACCCCAGG CATTTTCTTG GCCCCTGGTG CAGGGCTTCT AGAAGGAGCT CCAGAAGGTA 3121 TCCAGGAAGT CAGAAATATG GGACCCAGAA ATACTTCTGC TCACTCCAGA CCCTCATATC 3181 GTTCTGCTTC TTGGTCTTCT GATTCTGGTA GGCCCAAGAA TATGGGCACA CATCCTTCAG 3241 TCCAAAAAGA AGAAGCCTTT GAAATAATTG TTGAGTTTCC AGAAACCAAC TGCGATGTCA 3301 AAGACAGGCA GGGGAAAGAG CAGGGAGAAG AAATTAGTGA AAGAGGCGCA GGCCCTACAT 3361 TTAAAGCTAG TCCCTCCTGG GTTGACCCAG AAGGAGAAAC AGCAGAAAGC ACTGAAGATG 3421 CACCCTTAGA CTTTCACAGG GTCCTGCACA ATTCTCTGGG AAACATTTCC ATGCTGCCAT 3481 GTAGCTCCTT CACCCCTAAT TGGCCTGTTC AAAATCCTGA CTCCAGAAAA AGTGGTGGCC 3541 CAGTCGCAGA GCAGGGCATC GACCCTGATG CCTCCACAGT GGATGAGGAG GGGCAACTGC 3601 TCGACAGCAT GGATGTTCCC TGCACAAATG GGCACGGCTC TCATAGACTG TGCATTCTGA 3661 GACAGCCGCC TGGTCAGAGG GCGGAGACCC CCAATTCCTC TGTAAGCGGT AACATCCTCT 3721 TCCCTGTCCT CAGCGAGGAC TGCACTACCA CAGAGGAAGG AAATCAGCCT GGAAACATGC 3781 TAAACTGCAG CCAGAACTCC AGCTCATCCT CAGTGTGGTG GCTGAAATCA CCTGCATTTT 3841 CCAGTGGTTC TTCTGAGGGG GACAGCCCTT GGTCCTATCT GAATTCCAGT GGGAGTTCTT 3901 GGGTTTCATT GCCGGGAAAG ATGAGGAAAG AGATCCTTGA GGCTCGCACC TTGCAACCTG 3961 ATGACTTTGA AAAGCTGTTG GCAGGAGTGA GGCATGATTG GCTGTTTCAG AGACTAGAGA 4021 ATACGGGGGT TTTTAAGCCC AGTCAACTCC ACCGAGCACA TAGTGCTCTT TTGTTAAAAT 4081 ATTCAAAAAA ATCTGAACTG TGGACGGCCC AGGAAACTAT TGTCTATTTG GGGGACTACT 4141 TGACTGTGAA GAAAAAAGGC AGACAAAGAA ATGCTTTTTG GGTTCATCAT CTTCATCAAG 4201 AAGAAATTCT GGGGAGGTAT GTTGGGAAAG ACTATAAGGA GCAGAAGGGG CTCTGGCACC 4261 ACTTCACTGA TGTGGAGCGA CAGATGACCG CACAGCACTA TGTGACAGAA TTTAACAAGA 4321 GACTCTATGA ACAAAACATT CCCACCCAGA TATTCTACAT CCCATCCACA ATACTACTGA 4381 TTTTAGAGGA CAAGACAATA AAGGGATGTA TCAGTGTGGA GCCTTACATA CTGGGAGAAT 4441 TTGTAAAATT GTCAAATAAC ACGAAAGTGG TGAAAACAGA ATACAAAGCC ACAGAATATG 4501 GCTTGGCCTA TGGCCATTTT TCTTATGAGT TTTCTAATCA TAGAGATGTT GTGGTCGATT 4561 TACAAGGTTG GGTAACCGGT AATGGAAAAG GACTCATCTA CCTCACAGAT CCCCAGATTC 4621 ACTCCGTTGA TCAGAAAGTT TTCACTACCA ATTTTGGAAA GAGAGGAATT TTTTACTTCT 4681 TTAATAACCA GCATGTGGAA TGTAATGAAA TCTGCCATCG TCTTTCTTTG ACTAGACCTT 4741 CAATGGAGAA ACCATGCACA ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 4801 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 4861 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 4921 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 4981 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 5041 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 5101 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 5161 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 5221 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 5281 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 5341 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 5401 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 5461 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 5521 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 5581 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 5641 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 5701 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 5761 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 5821 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 5881 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 5941 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 6001 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 6061 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 6121 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 6181 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 6241 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 6301 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 6361 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 6421 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 6481 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 6541 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 6601 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 6661 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 6721 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 6781 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 6841 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 6901 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 6961 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 7021 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 7081 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 7141 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 7201 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 7261 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 7321 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 7381 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 7441 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 7501 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 7561 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 7621 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 7681 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 7741 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 7801 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 7861 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 7921 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 7981 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 8041 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 8101 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 8161 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 8221 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 8281 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 8341 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 8401 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 8461 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 8521 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 8581 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT //