LOCUS       RC209170                8919 bp    DNA     circular  SYN 
DEFINITION  GPR116 (Myc-DDK-tagged)-Human G protein-coupled receptor 116 (GPR116), transcript variant 1
ACCESSION   NM_015234
VERSION     NM_015234.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5076..5167 
                     /label="Myc-DDK"  
     misc_feature    5197..5782 
                     /label="PolyA signal"  
     misc_feature    5254..5273 
                     /label="Restriction Enzyme"  
     misc_feature    6058..6730 
                     /label="ColE1"  
     misc_feature    7160..7954 
                     /label="Kan/Neo"  
     misc_feature    7989..8291 
                     /label="SV40 ori"  
     misc_feature    8377..8854 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5058..5064 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5058 
                     /label="ADGRF5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAATCCCCA AGGAGAACCA CTTTGTGCCT CATGTTTATT GTGATTTATT 
     1081 CTTCCAAAGC TGCACTGAAC TGGAATTACG AGTCTACTAT TCATCCTTTG AGTCTTCATG 
     1141 AACATGAACC AGCTGGTGAA GAGGCACTGA GGCAAAAACG AGCCGTTGCC ACAAAAAGTC 
     1201 CTACGGCTGA AGAATACACT GTTAATATTG AGATCAGTTT TGAAAATGCA TCCTTCCTGG 
     1261 ATCCTATCAA AGCCTACTTG AACAGCCTCA GTTTTCCAAT TCATGGGAAT AACACTGACC 
     1321 AAATTACCGA CATTTTGAGC ATAAATGTGA CAACAGTCTG CAGACCTGCT GGAAATGAAA 
     1381 TCTGGTGCTC CTGCGAGACA GGTTATGGGT GGCCTCGGGA AAGGTGTCTT CACAATCTCA 
     1441 TTTGTCAAGA GCGTGACGTC TTCCTCCCAG GGCACCATTG CAGTTGCCTT AAAGAACTGC 
     1501 CTCCCAATGG ACCTTTTTGC CTGCTTCAGG AAGATGTTAC CCTGAACATG AGAGTCAGAC 
     1561 TAAATGTAGG CTTTCAAGAA GACCTCATGA ACACTTCCTC CGCCCTCTAT AGGTCCTACA 
     1621 AGACCGACTT GGAAACAGCG TTCCGGAAGG GTTACGGAAT TTTACCAGGC TTCAAGGGCG 
     1681 TGACTGTGAC AGGGTTCAAG TCTGGAAGTG TGGTTGTGAC ATATGAAGTC AAGACTACAC 
     1741 CACCATCACT TGAGTTAATA CATAAAGCCA ATGAACAAGT TGTACAGAGC CTCAATCAGA 
     1801 CCTACAAAAT GGACTACAAC TCCTTTCAAG CAGTTACTAT CAATGAAAGC AATTTCTTTG 
     1861 TCACACCAGA AATCATCTTT GAAGGGGACA CAGTCAGTCT GGTGTGTGAA AAGGAAGTTT 
     1921 TGTCCTCCAA TGTGTCTTGG CGCTATGAAG AACAGCAGTT GGAAATCCAG AACAGCAGCA 
     1981 GATTCTCGAT TTACACCGCA CTTTTCAACA ACATGACTTC GGTGTCCAAG CTCACCATCC 
     2041 ACAACATCAC TCCAGGTGAT GCAGGTGAAT ATGTTTGCAA ACTGATATTA GACATTTTTG 
     2101 AATATGAGTG CAAGAAGAAA ATAGATGTTA TGCCCATCCA AATTTTGGCA AATGAAGAAA 
     2161 TGAAGGTGAT GTGCGACAAC AATCCTGTAT CTTTGAACTG CTGCAGTCAG GGTAATGTTA 
     2221 ATTGGAGCAA AGTAGAATGG AAGCAGGAAG GAAAAATAAA TATTCCAGGA ACCCCTGAGA 
     2281 CAGACATAGA TTCTAGCTGC AGCAGATACA CCCTCAAGGC TGATGGAACC CAGTGCCCAA 
     2341 GCGGGTCGTC TGGAACAACA GTCATCTACA CTTGTGAGTT CATCAGTGCC TATGGAGCCA 
     2401 GAGGCAGTGC AAACATAAAA GTGACATTCA TCTCTGTGGC CAATCTAACA ATAACCCCGG 
     2461 ACCCAATTTC TGTTTCTGAG GGACAAAACT TTTCTATAAA ATGCATCAGT GATGTGAGTA 
     2521 ACTATGATGA GGTTTATTGG AACACTTCTG CTGGAATTAA AATATACCAA AGATTTTATA 
     2581 CCACGAGGAG GTATCTTGAT GGAGCAGAAT CAGTACTGAC AGTCAAGACC TCGACCAGGG 
     2641 AGTGGAATGG AACCTATCAC TGCATATTTA GATATAAGAA TTCATACAGT ATTGCAACCA 
     2701 AAGACGTCAT TGTTCACCCG CTGCCTCTAA AGCTGAACAT CATGGTTGAT CCTTTGGAAG 
     2761 CTACTGTTTC ATGCAGTGGT TCCCATCACA TCAAGTGCTG CATAGAGGAG GATGGAGACT 
     2821 ACAAAGTTAC TTTCCATACG GGTTCCTCAT CCCTTCCTGC TGCAAAAGAA GTTAACAAAA 
     2881 AACAAGTGTG CTACAAACAC AATTTCAATG CAAGCTCAGT TTCCTGGTGT TCAAAAACTG 
     2941 TTGATGTGTG TTGTCACTTT ACCAATGCTG CTAATAATTC AGTCTGGAGC CCATCTATGA 
     3001 AGCTGAATCT GGTTCCTGGG GAAAACATCA CATGCCAGGA TCCCGTAATA GGTGTCGGAG 
     3061 AGCCGGGGAA AGTCATCCAG AAGCTATGCC GGTTCTCAAA CGTTCCCAGC AGCCCTGAGA 
     3121 GTCCCATTGG CGGGACCATC ACTTACAAAT GTGTAGGCTC CCAGTGGGAG GAGAAGAGAA 
     3181 ATGACTGCAT CTCTGCCCCA ATAAACAGTC TGCTCCAGAT GGCTAAGGCT TTGATCAAGA 
     3241 GCCCCTCTCA GGATGAGATG CTCCCTACAT ACCTGAAGGA TCTTTCTATT AGCATAGACA 
     3301 AAGCGGAACA TGAAATCAGC TCTTCTCCTG GGAGTCTGGG AGCCATTATT AACATCCTTG 
     3361 ATCTGCTCTC AACAGTTCCA ACCCAAGTAA ATTCAGAAAT GATGACGCAC GTGCTCTCTA 
     3421 CGGTTAATGT CATCCTTGGC AAGCCCGTCT TGAACACCTG GAAGGTTTTA CAACAGCAAT 
     3481 GGACCAATCA GAGTTCACAG CTACTACATT CAGTGGAAAG ATTTTCCCAA GCATTACAGT 
     3541 CGGGAGATAG CCCTCCTTTG TCCTTCTCCC AAACTAATGT GCAGATGAGC AGCATGGTAA 
     3601 TCAAGTCCAG CCACCCAGAA ACCTATCAAC AGAGGTTTGT TTTCCCATAC TTTGACCTCT 
     3661 GGGGCAATGT GGTCATTGAC AAGAGCTATC TAGAAAACTT GCAGTCGGAT TCGTCTATTG 
     3721 TCACCATGGC TTTCCCAACT CTCCAAGCCA TCCTTGCCCA GGATATCCAG GAAAATAACT 
     3781 TTGCAGAGAG CTTAGTGATG ACAACCACTG TCAGCCACAA TACAACTATG CCATTCAGGA 
     3841 TTTCAATGAC TTTTAAGAAC AATAGCCCTT CAGGCGGCGA AACGAAGTGT GTCTTCTGGA 
     3901 ACTTCAGGCT TGCCAACAAC ACAGGGGGGT GGGACAGCAG TGGGTGCTAT GTAGAAGAAG 
     3961 GTGATGGGGA CAATGTCACC TGTATCTGTG ACCACCTAAC ATCATTCTCC ATCCTCATGT 
     4021 CCCCTGACTC CCCAGATCCT AGTTCTCTCC TGGGAATACT CCTGGATATT ATTTCTTATG 
     4081 TTGGGGTGGG CTTTTCCATC TTGAGCTTGG CAGCCTGTCT AGTTGTGGAA GCTGTGGTGT 
     4141 GGAAATCGGT GACCAAGAAC CGGACTTCTT ATATGCGCCA CACCTGCATA GTGAATATCG 
     4201 CTGCCTCCCT TCTGGTCGCC AACACCTGGT TCATTGTGGT CGCTGCCATC CAGGACAATC 
     4261 GCTACATACT CTGCAAGACA GCCTGTGTGG CTGCCACCTT CTTCATCCAC TTCTTCTACC 
     4321 TCAGCGTCTT CTTCTGGATG CTGACACTGG GCCTCATGCT GTTCTATCGC CTGGTTTTCA 
     4381 TTCTGCATGA AACAAGCAGG TCCACTCAGA AAGCCATTGC CTTCTGTCTT GGCTATGGCT 
     4441 GCCCACTTGC CATCTCGGTC ATCACGCTGG GAGCCACCCA GCCCCGGGAA GTCTATACGA 
     4501 GGAAGAATGT CTGTTGGCTC AACTGGGAGG ACACCAAGGC CCTGCTGGCT TTCGCCATCC 
     4561 CAGCACTGAT CATTGTGGTG GTGAACATAA CCATCACTAT TGTGGTCATC ACCAAGATCC 
     4621 TGAGGCCTTC CATTGGAGAC AAGCCATGCA AGCAGGAGAA GAGCAGCCTG TTTCAGATCA 
     4681 GCAAGAGCAT TGGGGTCCTC ACACCACTCT TGGGCCTCAC TTGGGGTTTT GGTCTCACCA 
     4741 CTGTGTTCCC AGGGACCAAC CTTGTGTTCC ATATCATATT TGCCATCCTC AATGTCTTCC 
     4801 AGGGATTATT CATTTTACTC TTTGGATGCC TCTGGGATCT GAAGGTACAG GAAGCTTTGC 
     4861 TGAATAAGTT TTCATTGTCG AGATGGTCTT CACAGCACTC AAAGTCAACA TCCCTGGGTT 
     4921 CATCCACACC TGTGTTTTCT ATGAGTTCTC CAATATCAAG GAGATTTAAC AATTTGTTTG 
     4981 GTAAAACAGG AACGTATAAT GTTTCCACCC CAGAAGCAAC CAGCTCATCC CTGGAAAACT 
     5041 CATCCAGTGC TTCTTCGTTG CTCAACACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     5101 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     5161 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     5221 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     5281 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     5341 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     5401 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     5461 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     5521 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5581 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5641 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5701 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5761 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5821 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5881 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5941 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     6001 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     6061 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     6121 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     6181 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     6241 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     6301 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     6361 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     6421 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     6481 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6541 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6601 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6661 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6721 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6781 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6841 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6901 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6961 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     7021 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     7081 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     7141 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     7201 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     7261 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     7321 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     7381 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     7441 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     7501 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7561 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7621 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7681 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7741 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7801 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7861 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7921 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7981 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     8041 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     8101 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     8161 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8221 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8281 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     8341 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     8401 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     8461 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     8521 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     8581 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8641 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8701 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8761 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8821 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8881 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//