LOCUS       RC209120                7248 bp    DNA     circular  SYN 
DEFINITION  PLCD3 (Myc-DDK-tagged)-Human phospholipase C, delta 3 (PLCD3)
ACCESSION   NM_133373
VERSION     NM_133373.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3405..3496 
                     /label="Myc-DDK"  
     misc_feature    3526..4111 
                     /label="PolyA signal"  
     misc_feature    3583..3602 
                     /label="Restriction Enzyme"  
     misc_feature    4387..5059 
                     /label="ColE1"  
     misc_feature    5489..6283 
                     /label="Kan/Neo"  
     misc_feature    6318..6620 
                     /label="SV40 ori"  
     misc_feature    6706..7183 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3387..3393 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3387 
                     /label="PLCD3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTGTGCGGC CGCTGGAGGC GTTGCCGCCG CCCGCCCGAG GAGCCCCCGG 
     1081 TGGCCGCCCA GGTCGCAGCC CAAGTCGCGG CGCCGGTCGC TCTCCCGTCC CCGCCGACTC 
     1141 CCTCCGATGG CGGCACCAAG AGGCCCGGGC TGCGGGCGCT GAAGAAGATG GGCCTGACGG 
     1201 AGGACGAGGA CGTGCGCGCC ATGCTGCGGG GCTCCCGGCT CCGCAAGATC CGCTCGCGCA 
     1261 CGTGGCACAA GGAGCGGCTG TACCGGCTGC AGGAGGACGG CCTGAGCGTG TGGTTCCAGC 
     1321 GGCGCATCCC GCGTGCGCCA TCGCAGCACA TCTTCTTCGT GCAGCACATC GAGGCGGTCC 
     1381 GCGAGGGCCA CCAGTCCGAG GGCCTGCGGC GCTTCGGGGG TGCCTTCGCG CCAGCGCGCT 
     1441 GCCTCACCAT CGCCTTCAAG GGCCGCCGCA AGAACCTGGA CCTGGCGGCG CCCACGGCTG 
     1501 AGGAAGCGCA GCGCTGGGTG CGCGGTCTGA CCAAGCTCCG CGCGCGCCTG GACGCCATGA 
     1561 GCCAGCGCGA GCGGCTAGAC CACTGGATCC ACTCCTATCT GCACCGGGCT GACTCCAACC 
     1621 AGGACAGCAA GATGAGCTTC AAGGAGATCA AGAGCCTGCT GAGAATGGTC AACGTGGACA 
     1681 TGAACGACAT GTACGCCTAC CTCCTCTTCA AGGAGTGTGA CCACTCCAAC AACGACCGTC 
     1741 TAGAGGGGGC TGAGATCGAG GAGTTCCTGC GGCGGCTGCT GAAGCGGCCG GAGCTGGAGG 
     1801 AGATCTTCCA TCAGTACTCG GGCGAGGACC GCGTGCTGAG TGCCCCTGAG CTGCTGGAGT 
     1861 TCCTGGAGGA CCAGGGCGAG GAGGGCGCCA CACTGGCCCG CGCCCAGCAG CTCATTCAGA 
     1921 CCTATGAGCT CAACGAGACA GCCAAGCAGC ATGAGCTGAT GACACTGGAT GGCTTCATGA 
     1981 TGTACCTGTT GTCGCCGGAG GGGGCTGCCT TGGACAACAC CCACACGTGT GTGTTCCAGG 
     2041 ACATGAACCA GCCCCTTGCC CACTACTTCA TCTCTTCCTC CCACAACACC TATCTGACTG 
     2101 ACTCCCAGAT CGGGGGGCCC AGCAGCACCG AGGCCTATGT TAGGGCCTTT GCCCAGGGAT 
     2161 GCCGCTGCGT GGAGCTGGAC TGCTGGGAGG GGCCAGGAGG GGAGCCCGTC ATCTATCATG 
     2221 GCCATACCCT CACCTCCAAG ATTCTCTTCC GGGACGTGGT CCAAGCCGTG CGCGACCATG 
     2281 CCTTCACGCT GTCCCCTTAC CCTGTCATCC TATCCCTGGA GAACCACTGC GGGCTGGAGC 
     2341 AGCAGGCTGC CATGGCCCGC CACCTCTGCA CCATCCTGGG GGACATGCTG GTGACACAGG 
     2401 CGCTGGACTC CCCAAATCCC GAGGAGCTGC CATCCCCAGA GCAGCTGAAG GGCCGGGTCC 
     2461 TGGTGAAGGG AAAGAAGCTG CCCGCTGCTC GGAGCGAGGA TGGCCGGGCT CTGTCGGATC 
     2521 GGGAGGAGGA GGAGGAGGAT GACGAGGAGG AAGAAGAGGA GGTGGAGGCT GCAGCGCAGA 
     2581 GGCGGCTGGC CAAGCAGATC TCCCCGGAGC TGTCGGCCCT GGCTGTGTAC TGCCACGCCA 
     2641 CCCGCCTGCG GACCCTGCAC CCTGCCCCCA ACGCCCCACA ACCCTGCCAG GTCAGCTCCC 
     2701 TCAGCGAGCG CAAAGCCAAG AAACTCATTC GGGAGGCAGG GAACAGCTTT GTCAGGCACA 
     2761 ATGCCCGCCA GCTGACCCGC GTGTACCCGC TGGGGCTGCG GATGAACTCA GCCAACTACA 
     2821 GTCCCCAGGA GATGTGGAAC TCGGGCTGTC AGCTGGTGGC CTTGAACTTC CAGACGCCAG 
     2881 GCTACGAGAT GGACCTCAAT GCCGGGCGCT TCCTAGTCAA TGGGCAGTGT GGCTACGTCC 
     2941 TAAAACCTGC CTGCCTGCGG CAACCTGACT CGACCTTTGA CCCCGAGTAC CCAGGACCTC 
     3001 CCAGAACCAC TCTCAGCATC CAGGTGCTGA CTGCACAGCA GCTGCCCAAG CTGAATGCCG 
     3061 AGAAGCCACA CTCCATTGTG GACCCCCTGG TGCGCATTGA GATCCATGGG GTGCCCGCAG 
     3121 ACTGTGCCCG GCAGGAGACT GACTACGTGC TCAACAATGG CTTCAACCCC CGCTGGGGGC 
     3181 AGACCCTGCA GTTCCAGCTG CGGGCTCCGG AGCTGGCACT GGTCCGGTTT GTGGTGGAAG 
     3241 ATTATGACGC CACCTCCCCC AATGACTTTG TGGGCCAGTT TACACTGCCT CTTAGCAGCC 
     3301 TAAAGCAAGG GTACCGCCAC ATACACCTGC TTTCCAAGGA CGGGGCCTCA CTGTCACCAG 
     3361 CCACGCTCTT CATCCAAATC CGCATCCAGC GCTCCACGCG TACGCGGCCG CTCGAGCAGA 
     3421 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     3481 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     3541 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     3601 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     3661 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     3721 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     3781 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     3841 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     3901 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     3961 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4021 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4081 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4141 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4201 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4261 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     4321 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     4381 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     4441 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     4501 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     4561 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     4621 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     4681 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     4741 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     4801 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     4861 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     4921 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     4981 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     5041 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5101 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5161 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     5221 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     5281 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     5341 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     5401 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     5461 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     5521 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     5581 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     5641 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     5701 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     5761 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     5821 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     5881 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     5941 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6001 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6061 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6121 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6181 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6241 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     6301 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     6361 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     6421 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     6481 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6541 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     6601 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     6661 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     6721 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     6781 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     6841 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     6901 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     6961 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7021 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7081 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7141 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7201 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//