LOCUS       RC209068                7707 bp    DNA     circular  SYN 
DEFINITION  DGKQ (Myc-DDK-tagged)-Human diacylglycerol kinase, theta 110kDa (DGKQ)
ACCESSION   NM_001347
VERSION     NM_001347.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3864..3955 
                     /label="Myc-DDK"  
     misc_feature    3985..4570 
                     /label="PolyA signal"  
     misc_feature    4042..4061 
                     /label="Restriction Enzyme"  
     misc_feature    4846..5518 
                     /label="ColE1"  
     misc_feature    5948..6742 
                     /label="Kan/Neo"  
     misc_feature    6777..7079 
                     /label="SV40 ori"  
     misc_feature    7165..7642 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3846..3852 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3846 
                     /label="DGKQ"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCGGCG GCCGAGCCCG GGGCCCGCGC CTGGCTGGGC GGCGGCTCCC 
     1081 CGCGCCCCGG CAGCCCGGCC TGCAGCCTCG TGCTGGGCTC AGGAGGCCGC GCGCGCCCGG 
     1141 GGCCGGGGCC GGGGCCGGGA CCCGAGCGGG CGGGCGTCAG AGCCCCGGGC CCCGCTGCCG 
     1201 CGCCGGGACA CAGCTTCCGG AAGGTGACGC TCACCAAGCC CACCTTCTGC CACCTCTGCT 
     1261 CCGACTTCAT CTGGGGGCTG GCCGGCTTCC TGTGCGACGT CTGCAATTTC ATGTCTCATG 
     1321 AGAAGTGCCT GAAGCACGTG AGGATCCCGT GCACGAGTGT GGCACCCAGC CTGGTCCGGG 
     1381 TTCCTGTAGC CCACTGCTTC GGCCCCCGGG GGCTCCACAA GCGCAAGTTC TGTGCTGTCT 
     1441 GCCGCAAGGT CCTGGAGGCA CCGGCGCTCC ACTGCGAAGT GTGTGAGCTG CACCTCCACC 
     1501 CAGACTGTGT GCCCTTCGCC TGCAGTGACT GCCGCCAGTG CCACCAGGAT GGGCACCAGG 
     1561 ATCACGACAC CCATCACCAC CACTGGCGGG AGGGGAACCT GCCCTCGGGA GCGCGCTGCG 
     1621 AGGTCTGCAG GAAGACGTGC GGCTCCTCTG ACGTGCTGGC CGGCGTGCGC TGCGAGTGGT 
     1681 GCGGGGTCCA GGCGCACTCC CTCTGCTCCG CGGCACTGGC TCCCGAGTGT GGCTTCGGGC 
     1741 GTCTGCGCTC CCTGGTCCTG CCTCCCGCGT GCGTGCGCCT TCTGCCCGGC GGCTTCAGCA 
     1801 AGACGCAGAG CTTCCGCATC GTGGAGGCCG CGGAGCCGGG CGAGGGGGGC GACGGCGCCG 
     1861 ACGGGAGCGC TGCCGTGGGT CCAGGCAGAG AGACACAGGC AACTCCGGAG TCCGGGAAGC 
     1921 AAACGCTGAA GATCTTTGAT GGCGACGACG CGGTGAGAAG AAGCCAGTTC CGCCTCGTCA 
     1981 CGGTGTCCCG CCTGGCCGGT GCCGAGGAGG TGCTGGAGGC CGCACTGCGG GCCCACCACA 
     2041 TCCCCGAGGA CCCTGGCCAC CTGGAGCTGT GCCGGCTGCC CCCTTCCTCT CAGGCCTGTG 
     2101 ACGCCTGGGC TGGGGGCAAG GCTGGGAGTG CTGTGATCTC GGAGGAGGGC AGAAGCCCCG 
     2161 GGTCCGGCGA GGCCACGCCA GAGGCCTGGG TCATCCGGGC TCTGCCGCGG GCCCAGGAGG 
     2221 TCCTGAAGAT CTACCCTGGC TGGCTCAAGG TGGGCGTGGC CTACGTGTCC GTGCGAGTGA 
     2281 CCCCGAAGAG CACGGCCCGC TCTGTGGTGC TGGAGGTCCT GCCGCTGCTC GGCCGCCAGG 
     2341 CCGAGAGTCC CGAGAGCTTC CAGCTGGTGG AGGTGGCGAT GGGCTGCAGG CACGTCCAGC 
     2401 GGACGATGCT GATGGACGAA CAGCCCCTGC TGGACCGGCT ACAGGACATC CGGCAGATGT 
     2461 CTGTGCGGCA GGTGAGCCAG ACGCGGTTCT ACGTGGCAGA GAGCAGGGAT GTAGCCCCGC 
     2521 ACGTCTCCCT GTTTGTTGGC GGCCTGCCTC CCGGCCTGTC TCCCGAGGAG TACAGCAGCC 
     2581 TGCTGCATGA GGCCGGGGCT ACCAAAGCCA CCGTGGTGTC CGTGAGTCAC ATCTACTCCT 
     2641 CCCAAGGCGC GGTAGTGTTG GACGTTGCCT GCTTTGCGGA GGCCGAGCGG CTGTACATGC 
     2701 TGCTGAAGGA CATGGCTGTG CGGGGCCGGC TGCTCACTGC CCTGGTGCTC CCCGACCTGC 
     2761 TGCACGCGAA GCTGCCCCCA GACAGCTGTC CCCTCCTTGT GTTCGTGAAC CCCAAGAGTG 
     2821 GAGGCCTCAA GGGCCGAGAC CTGCTCTGCA GCTTCCGGAA GCTACTGAAC CCTCATCAGG 
     2881 TCTTCGACCT GACCAACGGA GGTCCTCTTC CCGGGCTCCA CCTGTTCTCC CAGGTGCCCT 
     2941 GCTTCCGGGT GCTGGTGTGT GGTGGCGATG GCACTGTGGG CTGGGTGCTT GGCGCCCTGG 
     3001 AGGAGACACG GTACCGACTG GCCTGCCCGG AGCCTTCTGT GGCCATCCTG CCCCTGGGCA 
     3061 CAGGGAATGA CCTTGGTCGA GTCCTCCGCT GGGGGGCGGG CTACAGCGGC GAGGACCCGT 
     3121 TCTCCGTACT GCTGTCTGTG GACGAGGCCG ACGCCGTGCT CATGGACCGC TGGACCATCC 
     3181 TGCTGGATGC CCACGAGGCT GGCAGTGCAG AGAACGACAC GGCAGACGCA GAGCCCCCCA 
     3241 AGATCGTGCA GATGAGTAAC TACTGTGGCA TTGGCATCGA CGCGGAGCTG AGCCTGGACT 
     3301 TCCACCAGGC ACGGGAAGAG GAGCCTGGCA AGTTCACAAG CAGGCTGCAC AACAAGGGTG 
     3361 TGTACGTGCG GGTGGGGCTG CAGAAGATCA GTCACTCTCG GAGCCTGCAC AAGCAGATCC 
     3421 GGCTGCAGGT GGAGCGGCAG GAGGTGGAGC TGCCCAGTAT TGAAGGCCTC ATCTTCATCA 
     3481 ACATCCCCAG CTGGGGCTCG GGGGCCGACC TGTGGGGCTC CGACAGCGAC ACCAGGTTTG 
     3541 AGAAGCCACG CATGGACGAC GGGCTGCTGG AGGTTGTGGG CGTGACGGGC GTCGTGCACA 
     3601 TGGGCCAGGT CCAGGGTGGG CTGCGCTCCG GAATCCGGAT TGCCCAGGGT TCCTACTTCC 
     3661 GAGTCACGCT CCTCAAGGCC ACCCCGGTGC AGGTGGACGG GGAGCCCTGG GTCCAGGCCC 
     3721 CGGGGCACAT GATCATCTCA GCTGCTGGCC CTAAGGTGCA CATGCTGAGG AAGGCCAAGC 
     3781 AGAAGCCGAG GAGGGCCGGG ACCACCAGGG ATGCCCGGGC GGATGCTGCG CCTGCCCCTG 
     3841 AGAGCGATCC TAGGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     3901 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     3961 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4021 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4081 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4141 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4201 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4261 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4321 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4381 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4441 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     4501 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     4561 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     4621 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     4681 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     4741 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     4801 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     4861 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     4921 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     4981 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5041 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5101 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5161 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5221 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5281 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     5341 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     5401 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     5461 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     5521 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     5581 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     5641 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     5701 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     5761 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     5821 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     5881 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     5941 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6001 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6061 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6121 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6181 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6241 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6301 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     6361 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     6421 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     6481 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     6541 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     6601 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     6661 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     6721 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     6781 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     6841 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     6901 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     6961 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7021 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7081 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7141 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7201 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7261 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7321 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     7381 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     7441 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     7501 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     7561 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     7621 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     7681 ATTTAACAAA AATTTAACGC GAATTTT
//