LOCUS       RC209044                7122 bp    DNA     circular  SYN 
DEFINITION  MED25 (Myc-DDK-tagged)-Human mediator complex subunit 25 (MED25)
ACCESSION   NM_030973
VERSION     NM_030973.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3279..3370 
                     /label="Myc-DDK"  
     misc_feature    3400..3985 
                     /label="PolyA signal"  
     misc_feature    3457..3476 
                     /label="Restriction Enzyme"  
     misc_feature    4261..4933 
                     /label="ColE1"  
     misc_feature    5363..6157 
                     /label="Kan/Neo"  
     misc_feature    6192..6494 
                     /label="SV40 ori"  
     misc_feature    6580..7057 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3261..3267 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3261 
                     /label="MED25"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTCCCCGGG TCCGAGGGCC CGGCCCGCGC CGGGAGCGTG GTGGCCGACG 
     1081 TGGTGTTTGT GATTGAGGGT ACGGCCAACC TGGGACCCTA CTTCGAGGGG CTCCGCAAGC 
     1141 ACTACCTGCT CCCGGCCATC GAGTATTTTA ATGGTGGTCC TCCTGCTGAG ACGGACTTCG 
     1201 GGGGAGACTA TGGGGGGACC CAGTACAGCC TCGTGGTGTT CAACACAGTG GACTGCGCTC 
     1261 CCGAGTCCTA CGTACAATGT CACGCTCCCA CCAGCAGCGC CTATGAGTTT GTCACCTGGC 
     1321 TCGATGGCAT TAAGTTCATG GGCGGGGGTG GTGAGAGCTG CAGCCTCATC GCGGAAGGAC 
     1381 TCAGCACAGC CTTGCAGCTG TTTGATGACT TCAAGAAGAT GCGCGAGCAG ATTGGCCAGA 
     1441 CGCACCGGGT CTGCCTCCTC ATCTGCAACT CACCCCCATA CTTGTTGCCT GCTGTTGAGA 
     1501 GCACCACGTA CTCTGGATGC ACAACTGAGA ATCTTGTGCA GCAGATTGGG GAGCGGGGGA 
     1561 TCCACTTCTC CATTGTGTCT CCCCGGAAGC TGCCTGCGCT TCGGCTTCTG TTTGAGAAGG 
     1621 CAGCCCCCCC GGCCTTGCTG GAGCCGCTGC AGCCTCCGAC AGATGTGAGC CAGGACCCGA 
     1681 GGCACATGGT GCTGGTTCGG GGACTCGTGC TGCCTGTTGG GGGTGGCTCA GCCCCAGGCC 
     1741 CCCTCCAGTC AAAGCAGCCA GTCCCCCTGC CTCCCGCCGC ACCCTCAGGT GCCACTCTCT 
     1801 CAGCAGCCCC CCAGCAGCCT CTGCCCCCCG TCCCCCCGCA GTACCAGGTT CCCGGGAACC 
     1861 TGAGTGCAGC TCAGGTGGCC GCGCAGAATG CAGTGGAGGC TGCCAAGAAC CAGAAGGCTG 
     1921 GGCTGGGCCC TCGCTTCTCG CCCATCACCC CTCTCCAACA AGCTGCTCCC GGAGTGGGTC 
     1981 CCCCCTTCAG CCAGGCCCCA GCTCCCCAAC TACCCCCAGG ACCCCCTGGC GCCCCCAAGC 
     2041 CACCACCTGC TTCCCAGCCC AGTCTGGTCT CCACTGTGGC CCCTGGCTCC GGCCTGGCTC 
     2101 CCACGGCACA GCCCGGGGCA CCGTCCATGG CAGGCACTGT GGCCCCAGGA GGGGTGAGCG 
     2161 GCCCTTCCCC AGCCCAGCTG GGAGCCCCAG CCCTCGGTGG GCAGCAGTCA GTCTCCAATA 
     2221 AGCTTCTGGC CTGGAGCGGG GTCCTGGAGT GGCAAGAGAA ACCCAAACCT GCCTCAGTGG 
     2281 ATGCCAACAC CAAGCTGACG CGGTCACTGC CCTGCCAGGT CTACGTGAAT CATGGCGAGA 
     2341 ACCTGAAGAC GGAGCAGTGG CCCCAGAAGC TGATCATGCA GCTCATCCCC CAGCAGCTGC 
     2401 TGACCACCCT GGGCCCTTTG TTCCGGAACT CAAGGATGGT CCAGTTCCAT TTCACCAACA 
     2461 AGGACCTGGA GTCTCTCAAA GGCCTCTACC GCATCATGGG CAACGGCTTC GCGGGCTGCG 
     2521 TGCACTTCCC CCACACGGCG CCCTGTGAGG TGCGCGTGCT CATGCTCCTG TACTCGTCCA 
     2581 AGAAGAAGAT CTTCATGGGC CTCATCCCCT ACGACCAGAG CGGCTTCGTC AACGGCATCC 
     2641 GGCAGGTCAT CACCAACCAC AAGCAGGTCC AGCAGCAGAA GCTGGAGCAG CAGCAGCGAG 
     2701 GAATGGGGGG ACAGCAGGCA CCCCCAGGGC TGGGGCCCAT TCTGGAGGAC CAAGCCAGGC 
     2761 CCTCACAGAA TCTGCTCCAG CTCCGCCCAC CGCAGCCCCA GCCTCAGGGT ACCGTAGGGG 
     2821 CCTCTGGGGC CACGGGGCAG CCCCAGCCCC AAGGTACTGC CCAGCCCCCG CCAGGTGCCC 
     2881 CTCAAGGCCC TCCTGGAGCA GCTTCTGGCC CACCCCCTCC TGGACCCATC CTTCGGCCCC 
     2941 AGAACCCTGG GGCCAACCCT CAGCTGCGAA GCCTCCTCCT CAACCCACCA CCGCCGCAGA 
     3001 CTGGGGTGCC CCCACCCCAG GCCTCCCTCC ACCACCTCCA GCCACCAGGG GCTCCTGCGC 
     3061 TGCTGCCTCC GCCGCACCAG GGCCTGGGGC AGCCCCAGTT GGGGCCCCCA CTCCTGCATC 
     3121 CACCACCTGC CCAGTCCTGG CCCGCACAAC TTCCCCCTCG GGCTCCACTG CCAGGTCAGA 
     3181 TGCTGCTGAG CGGGGGTCCC CGGGGCCCGG TCCCCCAGCC GGGCCTGCAG CCCAGCGTCA 
     3241 TGGAGGACGA CATCCTCATG GATCTCATCA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     3301 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     3361 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     3421 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     3481 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     3541 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     3601 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     3661 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     3721 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     3781 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     3841 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     3901 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     3961 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4021 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     4081 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     4141 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     4201 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     4261 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     4321 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     4381 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     4441 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     4501 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     4561 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     4621 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     4681 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     4741 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     4801 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     4861 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     4921 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     4981 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     5041 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     5101 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     5161 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     5221 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     5281 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     5341 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     5401 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     5461 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     5521 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     5581 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     5641 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     5701 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     5761 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     5821 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     5881 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     5941 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6001 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     6061 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     6121 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     6181 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     6241 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     6301 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     6361 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6421 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6481 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     6541 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     6601 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     6661 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     6721 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     6781 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     6841 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     6901 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     6961 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7021 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     7081 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//