LOCUS       RC209027                8331 bp    DNA     circular  SYN 
DEFINITION  PPP2R3A (Myc-DDK-tagged)-Human protein phosphatase 2, regulatory subunit B'', alpha (PPP2R3A), transcript variant 1
ACCESSION   NM_002718
VERSION     NM_002718.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4488..4579 
                     /label="Myc-DDK"  
     misc_feature    4609..5194 
                     /label="PolyA signal"  
     misc_feature    4666..4685 
                     /label="Restriction Enzyme"  
     misc_feature    5470..6142 
                     /label="ColE1"  
     misc_feature    6572..7366 
                     /label="Kan/Neo"  
     misc_feature    7401..7703 
                     /label="SV40 ori"  
     misc_feature    7789..8266 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4470..4476 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4470 
                     /label="PPP2R3A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAGCAACT TACAGACTTG TGGTTAGTAC TGTGAACCAC TACAGCAGCG 
     1081 TGGTGATAGA CCGGCGTTTT GAACAAGCTA TACATTATTG CACTGGAACC TGCCACACCT 
     1141 TCACACATGG AATTGACTGC ATTGTGGTAC ACCATAGTGT TTGTGCAGAC CTCTTGCACA 
     1201 TCCCTGTGTC TCAGTTCAAA GATGCAGATC TGAACTCTAT GTTTCTACCC CATGAAAATG 
     1261 GGCTTTCTTC GGCTGAAGGA GACTATCCCC AACAGGCCTT CACAGGCATA CCCAGGGTCA 
     1321 AGAGAGGATC TACATTTCAG AATACCTACA ACTTAAAGGA TATTGCAGGA GAAGCAATCA 
     1381 GTTTTGCCAG TGGGAAAATA AAAGAATTTT CCTTTGAAAA ACTCAAAAAC TCTAACCATG 
     1441 CAGCTTACAG AAAGGGAAGG AAAGTTAAGT CTGACTCATT TAATAGGAGG TCAGTTGATT 
     1501 TGGACTTGCT TTGTGGCCAT TATAACAACG ATGGGAACGC CCCATCCTTT GGTTTACTGC 
     1561 GGAGTTCCTC AGTTGAGGAA AAACCTTTGT CTCATAGAAA CTCACTGGAT ACGAACCTGA 
     1621 CTTCCATGTT TCTTCAAAAC TTTTCTGAAG AAGACTTGGT TACTCAGATT TTGGAAAAAC 
     1681 ATAAAATAGA TAATTTTTCT TCTGGGACAG ACATAAAGAT GTGCTTGGAC ATCTTATTGA 
     1741 AATGCTCCGA GGATTTAAAA AAATGCACAG ACATCATAAA ACAATGCATA AAGAAAAAAT 
     1801 CAGGGAGTAG CATCAGTGAA GGAAGTGGTA ATGATACAAT TTCTAGCTCT GAAACTGTCT 
     1861 ATATGAATGT AATGACCAGG TTAGCATCCT ATCTGAAAAA GTTACCATTT GAATTCATGC 
     1921 AGTCTGGGAA TAATGAGGCT CTAGATTTAA CAGAACTGAT CAGTAATATG CCTAGCTTAC 
     1981 AACTGACTCC CTTCTCCCCA GTGTTTGGCA CTGAACAACC CCCTAAATAT GAAGATGTTG 
     2041 TCCAGCTCTC AGCTTCTGAC TCTGGACGAT TTCAAACTAT TGAATTGCAA AATGACAAGC 
     2101 CTAATTCTAG GAAGATGGAC ACTGTACAAT CCATTCCAAA CAACTCCACA AATTCCTTAT 
     2161 ATAACTTAGA GGTAAATGAT CCTAGAACTC TAAAAGCTGT CCAGGTCCAA TCACAGTCAT 
     2221 TAACCATGAA TCCTTTAGAA AATGTTTCTT CTGACGACTT AATGGAAACT CTTTATATTG 
     2281 AAGAAGAGTC AGATGGAAAG AAAGCATTAG ATAAAGGACA AAAGACAGAG AATGGACCTA 
     2341 GTCATGAGTT ATTAAAGGTA AATGAACATA GAGCAGAATT TCCAGAACAT GCTACTCATC 
     2401 TTAAAAAATG CCCCACCCCA ATGCAAAATG AAATTGGTAA GATATTTGAG AAATCATTTG 
     2461 TTAATCTACC TAAGGAAGAC TGTAAATCAA AAGTTTCTAA ATTTGAAGAG GGAGACCAGA 
     2521 GAGATTTTAC AAATTCCAGT AGCCAGGAAG AGATAGATAA ATTGTTAATG GATTTGGAAT 
     2581 CTTTTTCACA GAAGATGGAG ACCTCTCTAA GAGAGCCACT TGCGAAGGGT AAAAACTCTA 
     2641 ATTTTTTAAA TAGTCACAGT CAGTTGACCG GTCAGACCCT TGTAGATCTT GAGCCTAAAT 
     2701 CTAAAGTCTC TTCACCCATA GAAAAAGTCT CACCTTCCTG TCTAACAAGG ATTATTGAAA 
     2761 CCAATGGACA CAAAATAGAG GAAGAGGATC GAGCCCTCTT ACTGCGAATC CTGGAAAGCA 
     2821 TTGAAGACTT TGCTCAAGAA CTAGTTGAAT GCAAATCAAG CAGAGGGAGC CTATCACAAG 
     2881 AAAAGGAAAT GATGCAAATT CTACAGGAAA CCTTGACAAC TTCCTCCCAG GCCAATTTAT 
     2941 CAGTCTGTAG AAGTCCTGTT GGTGATAAAG CCAAAGATAC TACTTCAGCA GTTTTGATTC 
     3001 AGCAGACTCC AGAGGTGATC AAGATTCAAA ATAAACCAGA AAAGAAACCT GGAACACCAC 
     3061 TCCCACCTCC AGCCACCTCT CCAAGTAGTC CCCGACCTCT CTCCCCGGTT CCCCATGTGA 
     3121 ATAATGTTGT GAATGCGCCA TTGTCCATAA ACATTCCACG GTTCTACTTT CCTGAAGGAC 
     3181 TCCCAGATAC CTGTAGTAAT CATGAACAAA CTCTAAGCAG AATTGAAACT GCTTTCATGG 
     3241 ATATTGAAGA ACAGAAAGCA GACATTTATG AAATGGGGAA AATTGCAAAG GTCTGTGGCT 
     3301 GTCCTCTCTA TTGGAAAGCC CCCATGTTCA GGGCTGCAGG GGGAGAGAAG ACAGGATTTG 
     3361 TGACAGCACA GTCATTCATT GCCATGTGGA GAAAGTTGCT GAATAACCAT CATGATGATG 
     3421 CCTCTAAATT CATCTGTCTT CTAGCAAAGC CCAACTGCAG CTCTCTAGAA CAGGAGGATT 
     3481 TCATCCCTCT ACTTCAGGAT GTGGTGGATA CCCACCCTGG TCTCACGTTC CTGAAAGATG 
     3541 CTCCAGAATT CCACTCCCGC TACATCACCA CGGTTATTCA GAGAATATTC TACACAGTCA 
     3601 ACAGATCTTG GAGTGGAAAA ATTACTTCGA CAGAGATAAG AAAAAGCAAC TTTTTGCAAA 
     3661 CCCTAGCACT TTTGGAAGAA GAGGAAGATA TAAACCAAAT TACAGATTAC TTCTCCTATG 
     3721 AACATTTCTA TGTTATTTAT TGTAAATTCT GGGAACTAGA TACTGATCAC GACCTCTACA 
     3781 TCAGCCAGGC CGATCTGTCT CGATACAATG ACCAGGCTTC ATCAAGCAGG ATTATTGAAA 
     3841 GGATATTCTC TGGTGCAGTA ACAAGGGGAA AAACAATACA GAAAGAGGGA AGAATGAGCT 
     3901 ATGCAGATTT TGTTTGGTTT TTGATCTCTG AAGAAGACAA AAGGAATCCT ACCAGCATTG 
     3961 AGTATTGGTT CCGCTGCATG GATGTGGATG GAGACGGTGT ACTCTCCATG TATGAGCTGG 
     4021 AGTACTTCTA TGAGGAGCAG TGTGAACGGA TGGAAGCCAT GGGAATTGAG CCCTTGCCAT 
     4081 TCCATGATTT ACTGTGCCAG ATGCTTGACC TAGTGAAGCC AGCTGTTGAT GGCAAAATAA 
     4141 CTCTAAGAGA TCTGAAGAGG TGCAGAATGG CTCACATCTT CTATGACACT TTCTTTAATC 
     4201 TGGAGAAATA CTTAGACCAT GAACAGAGAG ATCCCTTTGC GGTCCAGAAG GATGTTGAGA 
     4261 ACGATGGGCC TGAGCCCTCA GACTGGGACC GGTTTGCCGC TGAGGAGTAT GAGACGCTTG 
     4321 TTGCAGAGGA ATCTGCCCAA GCACAATTCC AGGAAGGCTT TGAAGATTAT GAAACAGATG 
     4381 AACCTGCCTC TCCCTCTGAA TTTGGAAACA AAAGCAATAA AATATTAAGT GCAAGCCTTC 
     4441 CAGAGAAATG TGGAAAGCTT CAATCAGTGG ATGAAGAAAC GCGTACGCGG CCGCTCGAGC 
     4501 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4561 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4621 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4681 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4741 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4801 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4861 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4921 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4981 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5041 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5101 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5161 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5221 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5281 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5341 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5401 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5461 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5521 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5581 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5641 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5701 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5761 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5821 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5881 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5941 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6001 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6061 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6121 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6181 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6241 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6301 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6361 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6421 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6481 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6541 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6601 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6661 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6721 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6781 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6841 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6901 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6961 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7021 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7081 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7141 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7201 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7261 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7321 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7381 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7441 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7501 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7561 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7621 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7681 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7741 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7801 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7861 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7921 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7981 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8041 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8101 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8161 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8221 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8281 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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