LOCUS       RC209023                7137 bp    DNA     circular  SYN 
DEFINITION  TPCN2 (Myc-DDK-tagged)-Human two pore segment channel 2 (TPCN2)
ACCESSION   NM_139075
VERSION     NM_139075.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3294..3385 
                     /label="Myc-DDK"  
     misc_feature    3415..4000 
                     /label="PolyA signal"  
     misc_feature    3472..3491 
                     /label="Restriction Enzyme"  
     misc_feature    4276..4948 
                     /label="ColE1"  
     misc_feature    5378..6172 
                     /label="Kan/Neo"  
     misc_feature    6207..6509 
                     /label="SV40 ori"  
     misc_feature    6595..7072 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3276..3282 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3276 
                     /label="TPCN2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGAACCC CAGGCGGAGT CGGAGCCCCT GCTGGGCGGG GCCCGCGGCG 
     1081 GTGGCGGCGA CTGGCCGGCG GGGCTGACCA CTTACCGCAG CATCCAAGTC GGCCCTGGTG 
     1141 CCGCGGCCAG GTGGGACCTC TGCATTGATC AGGCTGTGGT CTTCATCGAA GATGCTATTC 
     1201 AGTACCGCTC CATCAACCAC CGGGTGGATG CCAGCTCGAT GTGGCTTTAC CGACGGTATT 
     1261 ACTCGAACGT ATGCCAACGG ACTTTGAGCT TCACCATCTT CTTGATCCTG TTTTTGGCTT 
     1321 TTATCGAGAC CCCATCCTCA CTCACCAGCA CGGCGGACGT GCGCTACCGC GCTGCCCCCT 
     1381 GGGAGCCGCC CTGCGGCCTG ACCGAGAGTG TCGAGGTGCT CTGCCTGCTG GTCTTTGCGG 
     1441 CCGACCTCTC TGTGAAGGGT TACCTGTTCG GGTGGGCCCA TTTCCAGAAA AACCTTTGGC 
     1501 TGCTGGGCTA CCTCGTGGTG CTGGTGGTGT CTCTGGTGGA CTGGACCGTG TCCCTGAGTC 
     1561 TCGTGTGTCA TGAGCCCCTG CGGATCCGCC GGCTTCTCCG TCCCTTCTTC CTGCTGCAGA 
     1621 ACTCCTCTAT GATGAAGAAG ACCTTGAAAT GCATCCGCTG GTCGCTGCCG GAAATGGCCA 
     1681 GCGTCGGGCT GCTGCTGGCC ATCCACCTGT GCCTCTTCAC CATGTTCGGA ATGCTGCTGT 
     1741 TCGCTGGTGG GAAGCAGGAT GATGGGCAGG ACAGGGAGAG GCTGACCTAC TTCCAGAACC 
     1801 TGCCTGAGTC TCTGACTTCC CTCCTGGTGC TGCTGACCAC GGCCAACAAC CCCGATGTGA 
     1861 TGATTCCTGC GTATTCCAAG AACCGGGCCT ATGCCATCTT CTTCATAGTC TTCACTGTGA 
     1921 TAGGAAGCCT GTTTCTGATG AACCTGCTGA CAGCCATCAT CTACAGTCAG TTCCGGGGCT 
     1981 ACCTGATGAA ATCTCTCCAG ACCTCGCTGT TTCGGAGGCG GCTGGGAACC CGGGCTGCCT 
     2041 TTGAAGTCCT ATCCTCCATG GTGGGGGAGG GAGGAGCCTT CCCTCAGGCA GTTGGGGTGA 
     2101 AGCCCCAGAA CTTGCTGCAG GTGCTTCAGA AGGTCCAGCT GGACAGCTCC CACAAACAGG 
     2161 CCATGATGGA GAAGGTGCGT TCCTACGGCA GTGTTCTGCT GTCAGCTGAG GAGTTTCAGA 
     2221 AGCTCTTCAA CGAGCTTGAC AGAAGTGTGG TTAAAGAGCA CCCGCCGAGG CCCGAGTACC 
     2281 AGTCTCCGTT TCTGCAGAGC GCCCAGTTCC TCTTCGGCCA CTACTACTTT GACTACCTGG 
     2341 GGAACCTCAT CGCCCTGGCA AACCTGGTGT CCATTTGCGT GTTCCTGGTG CTGGATGCAG 
     2401 ATGTGCTGCC TGCTGAGCGT GATGACTTCA TCCTGGGGAT TCTCAACTGC GTCTTCATTG 
     2461 TGTACTACCT GTTGGAGATG CTGCTCAAGG TCTTTGCCCT GGGCCTGCGA GGGTACCTGT 
     2521 CCTACCCCAG CAACGTGTTT GACGGGCTCC TCACCGTTGT CCTGCTGGTT TTGGAGATCT 
     2581 CAACTCTGGC TGTGTACCGA TTGCCACACC CAGGCTGGAG GCCGGAGATG GTGGGCCTGC 
     2641 TGTCGCTGTG GGACATGACC CGCATGCTGA ACATGCTCAT CGTGTTCCGC TTCCTGCGTA 
     2701 TCATCCCCAG CATGAAGCCG ATGGCCGTGG TGGCCAGTAC CGTCCTGGGC CTGGTGCAGA 
     2761 ACATGCGTGC TTTTGGCGGG ATCCTGGTGG TGGTCTACTA CGTATTTGCC ATCATTGGGA 
     2821 TCAACTTGTT TAGAGGCGTC ATTGTGGCTC TTCCTGGAAA CAGCAGCCTG GCCCCTGCCA 
     2881 ATGGCTCGGC GCCCTGTGGG AGCTTCGAGC AGCTGGAGTA CTGGGCCAAC AACTTCGATG 
     2941 ACTTTGCGGC TGCCCTGGTC ACTCTGTGGA ACTTGATGGT GGTGAACAAC TGGCAGGTGT 
     3001 TTCTGGATGC ATATCGGCGC TACTCAGGCC CGTGGTCCAA GATCTATTTT GTATTGTGGT 
     3061 GGCTGGTGTC GTCTGTCATC TGGGTCAACC TGTTTCTGGC CCTGATTCTG GAGAACTTCC 
     3121 TTCACAAGTG GGACCCCCGC AGCCACCTGC AGCCCCTTGC TGGGACCCCA GAGGCCACCT 
     3181 ACCAGATGAC TGTGGAGCTC CTGTTCAGGG ATATTCTGGA GGAGCCCGAG GAGGATGAGC 
     3241 TCACAGAGAG GCTGAGCCAG CACCCGCACC TGTGGCTGTG CAGGACGCGT ACGCGGCCGC 
     3301 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     3361 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     3421 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     3481 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     3541 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     3601 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     3661 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     3721 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     3781 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     3841 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     3901 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     3961 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4021 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     4081 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     4141 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     4201 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     4261 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     4321 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     4381 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     4441 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     4501 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     4561 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     4621 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     4681 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     4741 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     4801 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     4861 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     4921 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     4981 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     5041 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     5101 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     5161 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     5221 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     5281 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     5341 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     5401 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     5461 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     5521 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     5581 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     5641 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     5701 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     5761 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     5821 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     5881 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     5941 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     6001 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     6061 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     6121 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     6181 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     6241 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     6301 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     6361 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6421 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     6481 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     6541 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     6601 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     6661 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     6721 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     6781 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     6841 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     6901 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     6961 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     7021 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     7081 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//