LOCUS       RC208981                8487 bp    DNA     circular  SYN 
DEFINITION  CASKIN2 (Myc-DDK-tagged)-Human CASK interacting protein 2 (CASKIN2), transcript variant 1
ACCESSION   NM_020753
VERSION     NM_020753.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4644..4735 
                     /label="Myc-DDK"  
     misc_feature    4765..5350 
                     /label="PolyA signal"  
     misc_feature    4822..4841 
                     /label="Restriction Enzyme"  
     misc_feature    5626..6298 
                     /label="ColE1"  
     misc_feature    6728..7522 
                     /label="Kan/Neo"  
     misc_feature    7557..7859 
                     /label="SV40 ori"  
     misc_feature    7945..8422 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4626..4632 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4626 
                     /label="CASKIN2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGTCGTGAA CAGGACCTGA TCCTCGCCGT CAAGAATGGA GATGTGACCG 
     1081 GTGTGCAGAA ACTGGTGGCG AAGGTCAAGG CCACAAAGAC AAAGCTCCTG GGCTCCACAA 
     1141 AGAGGCTCAA CGTGAACTAC CAGGATGCTG ATGGATTCTC TGCCCTCCAC CACGCTGCTT 
     1201 TGGGGGGCAG CCTGGAGCTC ATAGCCTTGC TGCTAGAGGC TCAGGCCACT GTTGACATCA 
     1261 AGGACAGCAA TGGCATGCGC CCGCTGCACT ACGCAGCCTG GCAGGGCCGG CTGGAGCCTG 
     1321 TGAGGCTGCT GCTGCGCGCC TCTGCGGCTG TCAATGCCGC CTCGCTGGAC GGACAGATCC 
     1381 CCCTGCACCT GGCTGCACAG TATGGACATT ATGAGGTGTC AGAAATGCTC CTCCAGCATC 
     1441 AGTCCAACCC ATGCCTGGTC AACAAGGCCA AGAAGACGCC CCTGGACCTG GCCTGTGAAT 
     1501 TTGGCCGACT CAAGGTGGCC CAGCTGCTTC TGAACAGCCA CTTATGTGTG GCACTGCTGG 
     1561 AGGGTGAGGC CAAAGACCCG TGTGACCCCA ACTACACCAC GCCCCTGCAC TTGGCTGCCA 
     1621 AGAATGGCCA CAGAGAAGTC ATCAGGCAGC TCCTGAGAGC TGGGATCGAG ATCAACCGCC 
     1681 AGACCAAGAC GGGTACGGCG CTCCACGAGG CCGCACTGTA TGGCAAGACC GAGGTGGTGC 
     1741 GGCTGCTTCT GGAGGGAGGT GTGGACGTGA ACATCCGGAA TACGTATAAC CAGACGGCGC 
     1801 TGGACATAGT GAATCAGTTC ACCACCTCCC AGGCCAGCCG GGAAATCAAG CAGCTACTGC 
     1861 GGGAGGCCTC AGGGATCCTG AAGGTCCGAG CGCTCAAGGA TTTCTGGAAC CTCCACGATC 
     1921 CCACTGCTCT CAATGTCCGG GCAGGGGATG TCATCACGGT GCTAGAACAG CATCCCGACG 
     1981 GCCGCTGGAA GGGCCACATC CACGAGAGCC AGAGGGGCAC AGACCGCATA GGCTACTTCC 
     2041 CCCCGGGCAT TGTCGAGGTG GTCAGCAAGC GGGTGGGCAT CCCTGCAGCC CGCCTCCCCT 
     2101 CCGCACCCAC CCCCCTGCGC CCAGGCTTCT CCCGGACACC GCAGCCTCCT GCCGAAGAAC 
     2161 CCCCGCACCC TCTTACCTAC AGCCAGCTTC CTCGGGTGGG CCTCAGCCCA GACAGCCCAG 
     2221 CAGGTGACAG GAATAGTGTG GGCAGTGAGG GCAGCGTGGG CAGCATCCGC AGTGCCGGCA 
     2281 GCGGGCAGAG CTCTGAGGGC ACTAATGGCC ATGGCCCTGG CCTCCTGATT GAGAACGCCC 
     2341 AGCCACTGCC CTCTGCTGGA GAGGACCAGG TGCTGCCAGG ACTCCACCCG CCGTCCCTGG 
     2401 CAGACAACCT GAGCCACCGC CCTCTGGCCA ACTGCCGCTC TGGGGAGCAG ATCTTCACCC 
     2461 AGGACGTGCG GCCAGAACAG CTGCTGGAGG GGAAGGACGC GCAGGCCATT CATAACTGGC 
     2521 TAAGCGAGTT CCAGCTGGAG GGCTACACTG CCCACTTTCT GCAGGCCGGC TATGATGTGC 
     2581 CTACCATCAG CCGCATGACA CCTGAGGACC TGACGGCCAT CGGGGTGACC AAGCCTGGGC 
     2641 ACAGGAAGAA GATCGCCTCA GAGATCGCTC AGCTCAGCAT CGCCGAGTGG CTGCCCAGCT 
     2701 ACATCCCAAC GGACCTGCTG GAGTGGCTGT GTGCACTGGG GCTGCCACAG TACCACAAGC 
     2761 AGCTGGTGAG CAGCGGCTAC GACTCCATGG GGCTGGTGGC CGACCTCACC TGGGAGGAGC 
     2821 TGCAGGAGAT TGGGGTCAAC AAGCTCGGGC ATCAGAAGAA GCTCATGCTG GGGGTGAAGC 
     2881 GGCTGGCGGA GCTTCGGCGG GGCCTGCTGC AGGGGGAGGC CCTCAGCGAA GGCGGGCGCC 
     2941 GGCTGGCCAA GGGTCCGGAG CTGATGGCCA TCGAGGGACT GGAGAACGGA GAAGGCCCAG 
     3001 CTACAGCTGG CCCACGGCTC CTCACCTTCC AGGGCAGCGA ACTAAGCCCA GAGCTACAGG 
     3061 CGGCCATGGC AGGGGGTGGC CCTGAACCAC TCCCCCTCCC ACCTGCCCGC TCTCCCAGCC 
     3121 AGGAGAGCAT CGGGGCACGC TCACGGGGGT CTGGCCACTC ACAGGAACAG CCTGCCCCAC 
     3181 AGCCCAGCGG TGGAGATCCC AGCCCCCCCC AGGAGAGGAA CCTCCCAGAG GGCACAGAGC 
     3241 GGCCCCCTAA GCTTTGTTCT TCACTTCCTG GCCAAGGACC CCCACCCTAT GTTTTTATGT 
     3301 ACCCCCAGGG CTCACCCTCT AGCCCGGCCC CAGGGCCACC TCCTGGCGCA CCCTGGGCCT 
     3361 TCTCCTACTT GGCCGGGCCC CCTGCCACTC CCCCAGACCC GCCTCGACCT AAGCGCCGGT 
     3421 CCCACAGCCT AAGCCGCCCT GGCCCCACAG AGGGGGATGC TGAGGGGGAG GCCGAAGGGC 
     3481 CAGTGGGCAG CACCCTAGGC AGTTATGCTA CCCTTACCCG GCGGCCAGGA CGCAGTGCCC 
     3541 TTGTCCGGAC CAGTCCTAGT GTGACCCCAA CCCCAGCTCG GGGGACTCCT CGCAGCCAGT 
     3601 CCTTTGCCCT GCGGGCCCGG CGCAAAGGCC CCCCGCCCCC GCCCCCCAAG CGCCTCAGCT 
     3661 CCGTCTCTGG CCCCAGCCCG GAGCCACCTC CACTAGATGA GAGCCCAGGG CCCAAGGAAG 
     3721 GGGCCACAGG GCCCCGAAGG CGAACACTGA GTGAACCTGC TGGCCCCTCA GAGCCCCCTG 
     3781 GCCCACCTGC CCCGGCTGGG CCCGCGTCAG ACACGGAGGA GGAGGAGCCA GGCCCTGAGG 
     3841 GGACGCCCCC ATCTCGGGGC AGCTCTGGGG AAGGGCTGCC GTTTGCAGAG GAAGGGAACC 
     3901 TGACCATCAA ACAGCGCCCG AAGCCCGCTG GCCCCCCGCC CCGAGAGACA CCCGTGCCCC 
     3961 CCGGCCTCGA TTTCAACCTC ACGGAATCAG ACACTGTTAA GCGGAGGCCC AAGTGCCGGG 
     4021 AGAGAGAGCC ACTGCAGACC GCACTGCTGG CCTTCGGAGT GGCCAGTGCC ACGCCTGGCC 
     4081 CCGCTGCCCC ACTGCCTTCC CCAACTCCTG GCGAGTCTCC TCCAGCTTCT AGCCTTCCCC 
     4141 AGCCCGAGCC CAGCAGCCTT CCAGCCCAAG GAGTTCCAAC CCCCCTTGCT CCCAGCCCCG 
     4201 CCATGCAGCC TCCAGTGCCG CCCTGCCCAG GGCCAGGTCT GGAAAGCTCA GCAGCTAGTC 
     4261 GGTGGAATGG GGAGACAGAA CCCCCGGCCG CCCCTGCTGC CCTCCTCAAG GTGCCCGGAG 
     4321 CAGGAACAGC CCCCAAGCCT GTGTCGGTGG CCTGCACCCA GCTGGCATTT TCTGGCCCTA 
     4381 AGCTAGCGCC CCGGCTCGGC CCCCGCCCAG TGCCTCCTCC ACGGCCTGAG AGCACTGGGA 
     4441 CTGTGGGCCC AGGCCAGGCC CAGCAGAGAC TGGAGCAGAC CAGCTCGTCC CTGGCAGCTG 
     4501 CACTGAGAGC CGCAGAGAAG AGCATTGGCA CCAAGGAGCA AGAGGGCACC CCCAGCGCCT 
     4561 CCACCAAGCA CATTCTGGAT GACATCAGCA CCATGTTCGA CGCCCTGGCT GACCAGCTGG 
     4621 ACGCCATGCT GGACACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4681 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4741 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4801 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4861 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4921 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4981 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5041 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5101 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5161 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5221 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5281 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5341 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5401 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5461 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5521 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5581 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5641 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5701 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5761 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5821 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5881 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5941 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6001 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6061 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6121 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6181 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6241 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6301 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6361 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6421 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6481 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6541 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6601 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6661 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6721 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6781 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6841 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6901 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6961 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7021 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7081 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7141 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7201 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7261 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7321 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7381 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7441 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7501 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7561 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7621 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7681 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7741 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7801 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7861 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7921 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7981 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     8041 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8101 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8161 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8221 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8281 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8341 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8401 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8461 ATTTAACAAA AATTTAACGC GAATTTT
//