LOCUS RC208925 6747 bp DNA circular SYN DEFINITION ZDHHC13 (Myc-DDK-tagged)-Human zinc finger, DHHC-type containing 13 (ZDHHC13), transcript variant 1 ACCESSION NM_019028 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 2904..2995 /label="Myc-DDK" misc_feature 3025..3610 /label="PolyA signal" misc_feature 3082..3101 /label="Restriction Enzyme" misc_feature 3886..4558 /label="ColE1" misc_feature 4988..5782 /label="Kan/Neo" misc_feature 5817..6119 /label="SV40 ori" misc_feature 6205..6682 /label="f1 ori" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 2886..2892 /label="Restriction Enzyme" misc_feature 1020..2886 /label="ZDHHC13" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GGAGGGGCCG GGGCTGGGCT CGCAGTGCAG GAATCACAGC CATGGCCCCC 1081 ACCCTCCAGG ATTTGGTCGA TATGGCATCT GTGCACATGA AAACAAAGAA CTTGCCAATG 1141 CAAGAGAAGC TCTTCCTCTT ATAGAGGACT CTAGTAACTG TGACATTGTC AAAGCTACTC 1201 AATACGGAAT TTTTGAACGA TGTAAAGAGT TGGTAGAAGC AGGATATGAT GTCAGGCAAC 1261 CAGATAAAGA AAATGTGTCG CTTCTTCATT GGGCTGCTAT TAACAACAGA CTGGATCTTG 1321 TAAGGTTTTA TATTTCAAAA GGTGCTGTTG TAGATCAGTT GGGTGGAGAT TTAAATTCAA 1381 CTCCTCTTCA CTGGGCCATC CGACAAGGAC ATTTACCTAT GGTCATATTA TTACTCCAGC 1441 ATGGTGCAGA CCCCACTCTT ATTGATGGAG AGGGATTCAG CAGCATCCAC CTGGCAGTAT 1501 TGTTTCAACA CATGCCTATT ATAGCATATC TCATCTCAAA GGGACAGAGT GTGAATATGA 1561 CAGATGTAAA TGGGCAGACA CCTCTCATGT TATCAGCTCA CAAAGTAATT GGGCCAGAAC 1621 CAACTGGATT TCTTTTAAAG TTTAATCCTT CTCTCAATGT GGTTGATAAA ATACACCAAA 1681 ACACTCCACT TCACTGGGCA GTTGCAGCAG GAAATGTTAA TGCAGTTGAT AAGCTTTTGG 1741 AAGCTGGTTC TAGCCTGGAT ATCCAGAATG TTAAGGGAGA AACACCTCTT GATATGGCTC 1801 TACAAAACAA AAATCAGCTC ATTATTCATA TGCTAAAAAC AGAAGCCAAA ATGAGAGCCA 1861 ACCAAAAGTT CAGACTTTGG AGGTGGCTGC AGAAATGCGA GCTCTTCCTG CTGCTGATGC 1921 TTTCTGTGAT TACCATGTGG GCTATTGGAT ACATATTGGA CTTCAATTCA GATTCTTGGC 1981 TTTTAAAAGG ATGTCTTCTA GTAACACTGT TTTTTCTGAC ATCTTTGTTT CCAAGGTTCT 2041 TGGTTGGGTA TAAGAACCTT GTATACTTAC CAACAGCCTT TCTGCTAAGT TCTGTTTTTT 2101 GGATATTTAT GACTTGGTTC ATCTTATTTT TTCCTGATTT AGCAGGAGCC CCTTTCTATT 2161 TCAGTTTCAT TTTCAGCATA GTAGCCTTTC TATACTTTTT CTATAAGACT TGGGCAACTG 2221 ATCCAGGCTT CACTAAGGCT TCTGAAGAAG AAAAGAAAGT GAATATCATC ACCCTTGCAG 2281 AAACTGGCTC TCTGGACTTC AGGACATTTT GTACATCATG TCTTATAAGG AAGCCATTAA 2341 GGTCACTCCA CTGCCATGTA TGCAACTGCT GTGTGGCTCG ATATGATCAA CACTGCCTGT 2401 GGACTGGACG GTGCATAGGT TTTGGCAACC ATCACTATTA CATATTCTTC TTGTTTTTCC 2461 TTTCCATGGT ATGTGGCTGG ATTATATATG GATCTTTCAT CTATTTGTCC AGTCATTGTG 2521 CCACAACATT CAAAGAAGAT GGATTATGGA CTTACCTCAA TCAGATTGTG GCCTGTTCCC 2581 CTTGGGTTTT ATATATCTTG ATGCTAGCAA CTTTCCATTT CTCATGGTCA ACATTTTTAT 2641 TATTAAATCA ACTCTTTCAG ATTGCCTTTC TGGGCCTGAC CTCCCATGAG AGAATCAGCC 2701 TGCAGAAGCA GAGCAAGCAT ATGAAACAGA CGTTGTCCCT CAGGAAGACA CCATACAATC 2761 TTGGATTCAT GCAGAACCTG GCAGATTTCT TTCAGTGTGG CTGCTTTGGC TTGGTGAAGC 2821 CCTGTGTGGT AGATTGGACA TCACAGTATA CCATGGTCTT TCACCCAGCC AGGGAGAAGG 2881 TTCTTCGCTC AGTAACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 2941 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 3001 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 3061 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 3121 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 3181 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 3241 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 3301 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 3361 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 3421 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 3481 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 3541 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 3601 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 3661 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 3721 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 3781 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 3841 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 3901 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 3961 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 4021 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 4081 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 4141 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 4201 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 4261 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 4321 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 4381 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 4441 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 4501 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 4561 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 4621 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 4681 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 4741 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 4801 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 4861 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 4921 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 4981 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 5041 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 5101 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 5161 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 5221 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 5281 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 5341 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 5401 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 5461 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 5521 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 5581 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 5641 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 5701 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 5761 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 5821 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 5881 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 5941 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 6001 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 6061 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 6121 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 6181 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 6241 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 6301 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 6361 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 6421 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 6481 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 6541 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 6601 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 6661 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 6721 ATTTAACAAA AATTTAACGC GAATTTT //