LOCUS       RC208832                9198 bp    DNA     circular  SYN 
DEFINITION  AKNA (Myc-DDK-tagged)-Human AT-hook transcription factor (AKNA)
ACCESSION   NM_030767
VERSION     NM_030767.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5355..5446 
                     /label="Myc-DDK"  
     misc_feature    5476..6061 
                     /label="PolyA signal"  
     misc_feature    5533..5552 
                     /label="Restriction Enzyme"  
     misc_feature    6337..7009 
                     /label="ColE1"  
     misc_feature    7439..8233 
                     /label="Kan/Neo"  
     misc_feature    8268..8570 
                     /label="SV40 ori"  
     misc_feature    8656..9133 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5337..5343 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5337 
                     /label="AKNA"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCAGCTCG GAGACTGAGA TCCGCTGGGC TGAGCCTGGC CTGGGGAAGG 
     1081 GCCCCCAGCG GCGGCGCTGG GCCTGGGCCG AGGACAAGAG GGATGTGGAT AGAAGTAGTT 
     1141 CACAAAGCTG GGAAGAAGAG AGACTCTTTC CCAATGCCAC CAGCCCCGAG CTCCTAGAGG 
     1201 ACTTCCGCCT GGCCCAGCAG CACCTGCCGC CCCTGGAGTG GGACCCACAC CCGCAGCCCG 
     1261 ATGGGCATCA GGATTCCGAG TCAGGAGAGA CTTCGGGAGA AGAGGCTGAA GCAGAGGATG 
     1321 TGGACAGCCC AGCAAGTTCC CATGAGCCTC TTGCCTGGCT CCCCCAGCAG GGCCGTCAGC 
     1381 TGGACATGAC TGAAGAGGAG CCAGATGGGA CCCTCGGAAG TCTGGAGGTT GAGGAGGCTG 
     1441 GAGAGAGCTC CTCAAGGTTG GGGTATGAGG CTGGTCTCAG CTTGGAAGGC CATGGAAACA 
     1501 CCAGCCCCAT GGCTCTTGGG CATGGTCAGG CCAGGGGCTG GGTGGCTTCT GGCGAACAAG 
     1561 CCAGTGGGGA CAAACTTTCT GAACATTCCG AGGTCAACCC ATCCGTTGAA CTCAGCCCGG 
     1621 CAAGGTCCTG GAGCAGTGGG ACAGTGAGCC TCGACCACCC TAGTGACAGC CTTGATTCTA 
     1681 CCTGGGAAGG AGAGACCGAT GGCCCCCAGC CCACTGCCCT GGCAGAAACC TTGCCAGAGG 
     1741 GCCCCAGCCA CCACCTCCTA AGCCCAGATG GCAGAACTGG AGGCAGTGTT GCTCGGGCAA 
     1801 CCCCCATGGA ATTCCAGGAC TCCTCAGCTC CCCCAGCCCA GAGTCCGCAG CATGCCACAG 
     1861 ATAGATGGAG GAGAGAAACG ACCAGATTCT TCTGCCCTCA GCCCAAGGAA CACATCTGGA 
     1921 AGCAGACAAA GACGTCACCT AAGCCACTCC CTTCCCGATT CATTGGCTCC ATCAGCCCCC 
     1981 TGAATCCCCA GCCCAGGCCA ACGCGGCAGG GCAGGCCGCT GCCCAGACAG GGAGCCACTC 
     2041 TGGCTGGCCG CTCCTCTTCT AATGCCCCCA AGTATGGCCG GGGGCAGTTG AACTACCCAC 
     2101 TCCCTGATTT CTCCAAGGTA GGGCCCCGGG TGAGATTCCC CAAAGATGAG AGCTACCGTC 
     2161 CCCCCAAGTC CAGAAGCCAC AACAGGAAGC CTCAGGCCCC TGCCAGGCCC CTCATCTTCA 
     2221 AGTCTCCAGC TGAGATTGTG CAGGAGGTGC TGTTGAGCAG TGGAGAAGCA GCCCTGGCAA 
     2281 AGGACACGCC TCCTGCCCAC CCTATCACCA GGGTACCCCA AGAATTTCAG ACGCCTGAGC 
     2341 AAGCCACTGA GCTGGTCCAT CAGCTCCAGG AAGACTACCA CAGGCTCCTC ACCAAGTACG 
     2401 CTGAGGCCGA GAACACCATT GACCAGCTAC GCCTCGGGGC CAAGGTGAAC CTGTTCTCTG 
     2461 ACCCACCCCA GCCCAACCAC AGCATCCACA CGGGAATGGT GCCCCAGGGG ACCAAGGTCT 
     2521 TGTCCTTCAC CATCCCACAG CCCCGCTCTG CAGAGTGGTG GCCGGGCCCG GCCGAGGACC 
     2581 CCCAGGCCTC TGCGGCCTCA GGGTGGCCAT CAGCTCGAGG AGACTTGAGC CCCTCCTCGC 
     2641 TTACCAGCAT GCCCACCCTG GGGTGGCTTC CGGAGAACCG GGACATCTCT GAGGACCAGT 
     2701 CCTCAGCAGA GCAGACCCAG GCACTGGCTT CTCAGGCCAG CCAGTTCCTG GCCAAGGTGG 
     2761 AGTCCTTTGA AAGACTGATA CAGGCAGGAC GTCTCATGCC CCAGGACCAA GTCAAGGGCT 
     2821 TCCAGCGGCT GAAGGCTGCC CACGCGGCCC TAGAGGAGGA GTACCTGAAG GCTTGTCGGG 
     2881 AGCAACACCC TGCCCAGCCG CTTGCCGGCT CCAAGGGGAC GCCTGGAAGA TTTGATCCTC 
     2941 GCAGGGAGCT GGAGGCAGAG ATATACCGTC TGGGAAGCTG CCTGGAAGAG CTGAAGGAAC 
     3001 ACATAGACCA GACCCAGCAA GAGCCTGAGC CGCCCGGGTC AGACTCAGCT CTGGACAGCA 
     3061 CCCCAGCCCT GCCCTGCCTC CATCAGCCAA CGCACCTGCC TGCTCCTTCT GGACAAGCCC 
     3121 CCATGCCAGC CATCAAGACC TCCTGCCCTG AGCCTGCTAC CACCACTGCC GCCGCCAGCA 
     3181 CTGGCCCCTG CCCATTGCAC GTAAATGTGG AGGTGAGCTC TGGCAACAGT GAGGTGGAGG 
     3241 ACAGGCCACA GGACCCCCTG GCCCGACTCA GGCACAAGGA GCTGCAGATG GAGCAAGTTT 
     3301 ACCATGGCCT CATGGAGCGG TACCTCAGTG TGAAGTCTCT CCCAGAAGCC ATGAGAATGG 
     3361 AGGAGGAGGA AGAAGGAGAG GAGGAGGAGG AGGAAGAGGG GGGAGGTGAC TCCCTGGAAG 
     3421 TTGATGGGGT GGCTGCAACT CCAGGGAAAG CAGAGGCCAC CAGGGTCCTC CCAAGGCAGT 
     3481 GCCCGGTGCA GGCTGAGAAA AGTCATGGGG CTCCCCTGGA GGAGGCCACG GAGAAGATGG 
     3541 TATCTATGAA GCCACCAGGT TTCCAGGCAT CCCTGGCTAG AGACGGGCAC ATGTCAGGCC 
     3601 TGGGCAAGGC TGAGGCAGCC CCTCCAGGCC CTGGCGTGCC ACCCCACCCT CCAGGCACCA 
     3661 AGTCCGCAGC ATCCCACCAA AGTAGTATGA CCAGCCTGGA GGGAAGCGGC ATCTCTGAGC 
     3721 GCCTTCCACA GAAGCCTTTG CACCGAGGCG GTGGGCCCCA CCTGGAGGAG ACCTGGATGG 
     3781 CGTCCCCAGA GACAGACAGT GGCTTTGTGG GCTCAGAAAC AAGCAGAGTT TCACCCCTCA 
     3841 CCCAGACTCC AGAGCACCGG CTCTCCCACA TCAGCACAGC AGGAACATTA GCCCAGCCCT 
     3901 TTGCTGCATC TGTGCCCAGG GATGGAGCTT CCTACCCCAA GGCCAGGGGT TCTCTGATTC 
     3961 CCAGAAGAGC CACAGAGCCC AGCACACCCC GGAGCCAAGC ACAGAGGTAC CTCTCCAGCC 
     4021 CAAGTGGGCC TCTCCGGCAG AGGGCACCCA ACTTCAGCCT GGAGCGGACA CTGGCAGCCG 
     4081 AGATGGCGGT TCCTGGCTCA GAGTTTGAGG GGCACAAACG GATTTCTGAA CAGCCCCTTC 
     4141 CCAACAAGAC AATCAGCCCA CCCCCAGCCC CCGCCCCTGC CGCTGCGCCT CTACCCTGTG 
     4201 GACCAACAGA GACCATCCCC AGCTTCCTGC TCACCAGGGC AGGGCGAGAC CAGGCCATCT 
     4261 GTGAGCTGCA AGAAGAGGTG TCCCGGCTTC GTCTGCGGCT GGAAGACAGC CTGCACCAGC 
     4321 CACTCCAGGG CAGCCCGACA CGCCCAGCAT CTGCCTTTGA CCGCCCCGCC CGGACCCGCG 
     4381 GCCGGCCAGC AGACTCCCCA GCCACCTGGG GCTCCCATTA TGGCAGTAAA TCCACAGAGA 
     4441 GATTGCCTGG TGAGCCTAGA GGTGAAGAGC AGATTGTCCC TCCAGGAAGG CAGCGAGCCA 
     4501 GGTCTTCCTC AGTGCCTCGG GAGGTGCTCC GACTGTCCCT GAGTTCAGAA TCTGAGCTGC 
     4561 CCTCCCTACC ACTGTTCTCT GAGAAGAGCA AGACCACCAA GGACAGTCCA CAGGCAGCTC 
     4621 GGGATGGAAA GAGAGGGGTG GGCAGTGCTG GATGGCCAGA CAGGGTCACC TTCCGGGGCC 
     4681 AATACACAGG CCACGAATAC CATGTTCTGT CCCCTAAGGC GGTCCCAAAA GGCAATGGCA 
     4741 CAGTCTCCTG TCCCCACTGC CGGCCCATTA GGACCCAGGA TGCGGGTGGT GCTGTCACAG 
     4801 GGGACCCACT GGGACCGCCT CCCGCTGATA CCCTTCAGTG TCCCCTGTGT GGTCAAGTTG 
     4861 GGTCTCCCCC AGAGGCAGAT GGTCCAGGCT CAGCCACCTC TGGGGCAGAG AAGGCCACCA 
     4921 CGAGGAGAAA AGCATCTTCA ACTCCCAGCC CCAAGCAGAG GAGCAAGCAG GCGGGGTCGT 
     4981 CGCCACGCCC ACCCCCCGGA CTGTGGTATC TGGCAACAGC GCCCCCAGCA CCAGCCCCTC 
     5041 CAGCCTTTGC CTACATCTCC TCGGTTCCCA TCATGCCTTA TCCACCTGCC GCTGTGTACT 
     5101 ATGCGCCTGC AGGACCTACC TCAGCCCAAC CAGCTGCCAA GTGGCCGCCC ACAGCCTCTC 
     5161 CCCCACCAGC CCGGAGACAC CGGCACTCCA TCCAGCTCGA CCTGGGCGAC CTAGAGGAGC 
     5221 TCAACAAGGC CCTGAGCCGG GCCGTGCAGG CTGCCGAGAG CGTCCGCTCT ACCACCAGGC 
     5281 AGATGAGAAG CTCGCTGTCA GCCGACCTGC GCCAGGCTCA CAGCCTGCGG GGCTCCTGCC 
     5341 TCTTCACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     5401 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     5461 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     5521 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     5581 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     5641 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5701 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5761 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5821 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5881 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5941 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     6001 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     6061 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     6121 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     6181 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     6241 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     6301 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     6361 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     6421 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     6481 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     6541 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     6601 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6661 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6721 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6781 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6841 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6901 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6961 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     7021 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     7081 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     7141 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     7201 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     7261 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     7321 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     7381 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     7441 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     7501 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     7561 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     7621 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7681 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7741 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7801 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7861 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7921 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7981 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     8041 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     8101 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     8161 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     8221 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     8281 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     8341 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     8401 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     8461 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8521 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8581 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     8641 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8701 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8761 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8821 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8881 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8941 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     9001 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     9061 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     9121 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     9181 AAATTTAACG CGAATTTT
//