LOCUS       RC208765                8607 bp    DNA     circular  SYN 
DEFINITION  IRS1 (Myc-DDK-tagged)-Human insulin receptor substrate 1 (IRS1)
ACCESSION   NM_005544
VERSION     NM_005544.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4764..4855 
                     /label="Myc-DDK"  
     misc_feature    4885..5470 
                     /label="PolyA signal"  
     misc_feature    4942..4961 
                     /label="Restriction Enzyme"  
     misc_feature    5746..6418 
                     /label="ColE1"  
     misc_feature    6848..7642 
                     /label="Kan/Neo"  
     misc_feature    7677..7979 
                     /label="SV40 ori"  
     misc_feature    8065..8542 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4746..4752 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4746 
                     /label="IRS1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGAGCCCT CCGGAGAGCG ATGGCTTCTC GGACGTGCGC AAGGTGGGCT 
     1081 ACCTGCGCAA ACCCAAGAGC ATGCACAAAC GCTTCTTCGT ACTGCGCGCG GCCAGCGAGG 
     1141 CTGGGGGCCC GGCGCGCCTC GAGTACTACG AGAACGAGAA GAAGTGGCGG CACAAGTCGA 
     1201 GCGCCCCCAA ACGCTCGATC CCCCTTGAGA GCTGCTTCAA CATCAACAAG CGGGCTGACT 
     1261 CCAAGAACAA GCACCTGGTG GCTCTCTACA CCCGGGACGA GCACTTTGCC ATCGCGGCGG 
     1321 ACAGCGAGGC CGAGCAAGAC AGCTGGTACC AGGCTCTCCT ACAGCTGCAC AACCGTGCTA 
     1381 AGGGCCACCA CGACGGAGCT GCGGCCCTCG GGGCGGGAGG TGGTGGGGGC AGCTGCAGCG 
     1441 GCAGCTCCGG CCTTGGTGAG GCTGGGGAGG ACTTGAGCTA CGGTGACGTG CCCCCAGGAC 
     1501 CCGCATTCAA AGAGGTCTGG CAAGTGATCC TGAAGCCCAA GGGCCTGGGT CAGACAAAGA 
     1561 ACCTGATTGG TATCTACCGC CTTTGCCTGA CCAGCAAGAC CATCAGCTTC GTGAAGCTGA 
     1621 ACTCGGAGGC AGCGGCCGTG GTGCTGCAGC TGATGAACAT CAGGCGCTGT GGCCACTCGG 
     1681 AAAACTTCTT CTTCATCGAG GTGGGCCGTT CTGCCGTGAC GGGGCCCGGG GAGTTCTGGA 
     1741 TGCAGGTGGA TGACTCTGTG GTGGCCCAGA ACATGCACGA GACCATCCTG GAGGCCATGC 
     1801 GGGCCATGAG TGATGAGTTC CGCCCTCGCA GCAAGAGCCA GTCCTCGTCC AACTGCTCTA 
     1861 ACCCCATCAG CGTCCCCCTG CGCCGGCACC ATCTCAACAA TCCCCCGCCC AGCCAGGTGG 
     1921 GGCTGACCCG CCGATCACGC ACTGAGAGCA TCACCGCCAC CTCCCCGGCC AGCATGGTGG 
     1981 GCGGGAAGCC AGGCTCCTTC CGTGTCCGCG CCTCCAGTGA CGGCGAAGGC ACCATGTCCC 
     2041 GCCCAGCCTC GGTGGACGGC AGCCCTGTGA GTCCCAGCAC CAACAGAACC CACGCCCACC 
     2101 GGCATCGGGG CAGCGCCCGG CTGCACCCCC CGCTCAACCA CAGCCGCTCC ATCCCCATGC 
     2161 CGGCTTCCCG CTGCTCGCCT TCGGCCACCA GCCCGGTCAG TCTGTCGTCC AGTAGCACCA 
     2221 GTGGCCATGG CTCCACCTCG GATTGTCTCT TCCCACGGCG ATCTAGTGCT TCGGTGTCTG 
     2281 GTTCCCCCAG CGATGGCGGT TTCATCTCCT CGGATGAGTA TGGCTCCAGT CCCTGCGATT 
     2341 TCCGGAGTTC CTTCCGCAGT GTCACTCCGG ATTCCCTGGG CCACACCCCA CCAGCCCGCG 
     2401 GTGAGGAGGA GCTAAGCAAC TATATCTGCA TGGGTGGCAA GGGGCCCTCC ACCCTGACCG 
     2461 CCCCCAACGG TCACTACATT TTGTCTCGGG GTGGCAATGG CCACCGCTGC ACCCCAGGAA 
     2521 CAGGCTTGGG CACGAGTCCA GCCTTGGCTG GGGATGAAGC AGCCAGTGCT GCAGATCTGG 
     2581 ATAATCGGTT CCGAAAGAGA ACTCACTCGG CAGGCACATC CCCTACCATT ACCCACCAGA 
     2641 AGACCCCGTC CCAGTCCTCA GTGGCTTCCA TTGAGGAGTA CACAGAGATG ATGCCTGCCT 
     2701 ACCCACCAGG AGGTGGCAGT GGAGGCCGAC TGCCGGGACA CAGGCACTCC GCCTTCGTGC 
     2761 CCACCCGCTC CTACCCAGAG GAGGGTCTGG AAATGCACCC CTTGGAGCGT CGGGGGGGGC 
     2821 ACCACCGCCC AGACAGCTCC ACCCTCCACA CGGATGATGG CTACATGCCC ATGTCCCCAG 
     2881 GGGTGGCCCC AGTGCCCAGT GGCCGAAAGG GCAGTGGAGA CTATATGCCC ATGAGCCCCA 
     2941 AGAGCGTATC TGCCCCACAG CAGATCATCA ATCCCATCAG ACGCCATCCC CAGAGAGTGG 
     3001 ACCCCAATGG CTACATGATG ATGTCCCCCA GCGGTGGCTG CTCTCCTGAC ATTGGAGGTG 
     3061 GCCCCAGCAG CAGCAGCAGC AGCAGCAACG CCGTCCCTTC CGGGACCAGC TATGGAAAGC 
     3121 TGTGGACAAA CGGGGTAGGG GGCCACCACT CTCATGTCTT GCCTCACCCC AAACCCCCAG 
     3181 TGGAGAGCAG CGGTGGTAAG CTCTTACCTT GCACAGGTGA CTACATGAAC ATGTCACCAG 
     3241 TGGGGGACTC CAACACCAGC AGCCCCTCCG ACTGCTACTA CGGCCCTGAG GACCCCCAGC 
     3301 ACAAGCCAGT CCTCTCCTAC TACTCATTGC CAAGATCCTT TAAGCACACC CAGCGCCCCG 
     3361 GGGAGCCGGA GGAGGGTGCC CGGCATCAGC ACCTCCGCCT TTCCACTAGC TCTGGTCGCC 
     3421 TTCTCTATGC TGCAACAGCA GATGATTCTT CCTCTTCCAC CAGCAGCGAC AGCCTGGGTG 
     3481 GGGGATACTG CGGGGCTAGG CTGGAGCCCA GCCTTCCACA TCCCCACCAT CAGGTTCTGC 
     3541 AGCCCCATCT GCCTCGAAAG GTGGACACAG CTGCTCAGAC CAATAGCCGC CTGGCCCGGC 
     3601 CCACGAGGCT GTCCCTGGGG GATCCCAAGG CCAGCACCTT ACCTCGGGCC CGAGAGCAGC 
     3661 AGCAGCAGCA GCAGCCCTTG CTGCACCCTC CAGAGCCCAA GAGCCCGGGG GAATATGTCA 
     3721 ATATTGAATT TGGGAGTGAT CAGTCTGGCT ACTTGTCTGG CCCGGTGGCT TTCCACAGCT 
     3781 CACCTTCTGT CAGGTGTCCA TCCCAGCTCC AGCCAGCTCC CAGAGAGGAA GAGACTGGCA 
     3841 CTGAGGAGTA CATGAAGATG GACCTGGGGC CGGGCCGGAG GGCAGCCTGG CAGGAGAGCA 
     3901 CTGGGGTCGA GATGGGCAGA CTGGGCCCTG CACCTCCCGG GGCTGCTAGC ATTTGCAGGC 
     3961 CTACCCGGGC AGTGCCCAGC AGCCGGGGTG ACTACATGAC CATGCAGATG AGTTGTCCCC 
     4021 GTCAGAGCTA CGTGGACACC TCGCCAGCTG CCCCTGTAAG CTATGCTGAC ATGCGAACAG 
     4081 GCATTGCTGC AGAGGAGGTG AGCCTGCCCA GGGCCACCAT GGCTGCTGCC TCCTCATCCT 
     4141 CAGCAGCCTC TGCTTCCCCG ACTGGGCCTC AAGGGGCAGC AGAGCTGGCT GCCCACTCGT 
     4201 CCCTGCTGGG GGGCCCACAA GGACCTGGGG GCATGAGCGC CTTCACCCGG GTGAACCTCA 
     4261 GTCCTAACCG CAACCAGAGT GCCAAAGTGA TCCGTGCAGA CCCACAAGGG TGCCGGCGGA 
     4321 GGCATAGCTC CGAGACTTTC TCCTCAACAC CCAGTGCCAC CCGGGTGGGC AACACAGTGC 
     4381 CCTTTGGAGC GGGGGCAGCA GTAGGGGGCG GTGGCGGTAG CAGCAGCAGC AGCGAGGATG 
     4441 TGAAACGCCA CAGCTCTGCT TCCTTTGAGA ATGTGTGGCT GAGGCCTGGG GAGCTTGGGG 
     4501 GAGCCCCCAA GGAGCCAGCC AAACTGTGTG GGGCTGCTGG GGGTTTGGAG AATGGTCTTA 
     4561 ACTACATAGA CCTGGATTTG GTCAAGGACT TCAAACAGTG CCCTCAGGAG TGCACCCCTG 
     4621 AACCGCAGCC TCCCCCACCC CCACCCCCTC ATCAACCCCT GGGCAGCGGT GAGAGCAGCT 
     4681 CCACCCGCCG CTCAAGTGAG GATTTAAGCG CCTATGCCAG CATCAGTTTC CAGAAGCAGC 
     4741 CAGAGGACCG TCAGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4801 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4861 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4921 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4981 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     5041 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     5101 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5161 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5221 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5281 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5341 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5401 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5461 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5521 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5581 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5641 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5701 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5761 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5821 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5881 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5941 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     6001 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     6061 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6121 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6181 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6241 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6301 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6361 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6421 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6481 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6541 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6601 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6661 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6721 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6781 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6841 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6901 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6961 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     7021 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     7081 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7141 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7201 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7261 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7321 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7381 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7441 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7501 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7561 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7621 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7681 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7741 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7801 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7861 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7921 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7981 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     8041 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     8101 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     8161 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8221 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8281 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8341 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8401 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8461 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8521 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8581 ATTTAACAAA AATTTAACGC GAATTTT
//