LOCUS       RC208691                9465 bp    DNA     circular  SYN 
DEFINITION  DLC1 (Myc-DDK-tagged)-Human deleted in liver cancer 1 (DLC1), transcript variant 1
ACCESSION   NM_182643
VERSION     NM_182643.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5622..5713 
                     /label="Myc-DDK"  
     misc_feature    5743..6328 
                     /label="PolyA signal"  
     misc_feature    5800..5819 
                     /label="Restriction Enzyme"  
     misc_feature    6604..7276 
                     /label="ColE1"  
     misc_feature    7706..8500 
                     /label="Kan/Neo"  
     misc_feature    8535..8837 
                     /label="SV40 ori"  
     misc_feature    8923..9400 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5604..5610 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5604 
                     /label="DLC1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTGTAGCT ATCAGAAAGA GAAGCTGGGA AGAACATGTG ACCCACTGGA 
     1081 TGGGACAGCC TTTTAATTCT GATGATCGTA ACACAGCATG TCATCATGGA CTAGTAGCTG 
     1141 ACAGCTTGCA GGCAAGTATG GAAAAAGATG CAACTCTAAA TGTGGACCGC AAAGAGAAGT 
     1201 GTGTTTCACT ACCTGACTGC TGTCATGGAT CAGAGCTGAG AGATTTTCCT GGGAGGCCAA 
     1261 TGGGTCATCT TTCAAAGGAT GTGGACGAAA ATGACAGCCA TGAAGGTGAA GATCAGTTTC 
     1321 TTTCTCTGGA AGCCAGCACA GAAACACTAG TGCATGTTTC TGATGAGGAT AACAATGCTG 
     1381 ATTTATGCCT TACAGATGAT AAACAGGTTT TAAATACCCA AGGGCAGAAA ACATCAGGCC 
     1441 AACATATGAT CCAAGGAGCA GGCTCCTTAG AAAAGGCACT GCCCATCATA CAAAGTAACC 
     1501 AAGTTTCTTC TAACTCCTGG GGAATAGCTG GTGAAACTGA ATTAGCACTG GTAAAAGAAA 
     1561 GTGGGGAGAG AAAAGTTACT GACTCTATAA GTAAAAGCCT GGAGCTTTGC AATGAAATAA 
     1621 GCTTAAGTGA AATAAAAGAT GCACCCAAAG TAAATGCAGT GGATACTTTG AACGTGAAAG 
     1681 ATATTGCACC TGAGAAACAA TTGCTTAACT CTGCTGTAAT TGCTCAGCAA CGAAGGAAAC 
     1741 CTGACCCCCC TAAAGATGAA AATGAAAGAA GCACCTGCAA TGTAGTACAA GATGAGTTCT 
     1801 TGGATACTCC TTGCACAAAC AGAGGACTGC CATTATTAAA AACAGATTTT GGAAGCTGCC 
     1861 TTCTGCAGCC TCCTTCCTGC CCCAATGGAA TGTCAGCTGA AAATGGCCTG GAGAAGAGTG 
     1921 GTTTTTCACA ACATCAAAAC AAAAGTCCAC CAAAGGTCAA GGCAGAAGAT GGCATGCAGT 
     1981 GTTTACAATT AAAGGAGACC CTGGCCACCC AGGAACCCAC AGATAACCAA GTCAGACTTC 
     2041 GTAAGAGAAA GGAAATAAGA GAAGATCGAG ATAGGGCGCG GCTGGACTCC ATGGTGCTGC 
     2101 TGATTATGAA ACTGGACCAG CTTGATCAGG ACATAGAAAA TGCCCTCAGC ACCAGCTCCT 
     2161 CTCCATCAGG CACACCAACA AACCTGCGGC GGCACGTTCC TGATCTGGAA TCAGGATCTG 
     2221 AAAGTGGAGC AGATACCATT TCAGTAAATC AGACACGAGT AAATTTGTCT TCTGACACTG 
     2281 AGTCCACGGA CCTCCCATCT TCCACTCCAG TAGCCAATTC TGGAACCAAA CCCAAGACTA 
     2341 CGGCTATTCA AGGTATTTCA GAGAAGGAAA AGGCTGAAAT TGAAGCCAAG GAAGCTTGTG 
     2401 ATTGGCTACG GGCAACTGGT TTCCCCCAGT ATGCACAGCT TTATGAAGAT TTCCTGTTCC 
     2461 CCATCGATAT TTCCTTGGTC AAGAGAGAGC ATGATTTTTT GGACAGAGAT GCCATTGAGG 
     2521 CTCTATGCAG GCGTCTAAAT ACTTTAAACA AATGTGCGGT GATGAAGCTA GAAATTAGTC 
     2581 CTCATCGGAA ACGAAGTGAC GATTCAGACG AGGATGAGCC TTGTGCCATC AGTGGCAAAT 
     2641 GGACTTTCCA AAGGGACAGC AAGAGGTGGT CCCGGCTTGA AGAGTTTGAT GTCTTTTCTC 
     2701 CAAAACAAGA CCTGGTCCCT GGGTCCCCAG ACGACTCCCA CCCGAAGGAC GGCCCCAGCC 
     2761 CCGGAGGCAC GCTGATGGAC CTCAGCGAGC GCCAGGAGGT GTCTTCCGTC CGCAGCCTCA 
     2821 GCAGCACTGG CAGCCTCCCC AGCCACGCTC CCCCCAGCGA GGATGCTGCC ACCCCCCGGA 
     2881 CTAACTCCGT CATCAGCGTT TGCTCCTCCA GCAACTTGGC AGGCAATGAC GACTCTTTCG 
     2941 GCAGCCTGCC CTCTCCCAAG GAACTGTCCA GCTTCAGCTT CAGCATGAAA GGCCACGAAA 
     3001 AAACTGCCAA GTCCAAGACG CGCAGTCTGC TGAAACGGAT GGAGAGCCTG AAGCTCAAGA 
     3061 GCTCCCATCA CAGCAAGCAC AAAGCGCCCT CAAAGCTGGG GTTGATCATC AGCGGGCCCA 
     3121 TCTTGCAAGA GGGGATGGAT GAGGAGAAGC TGAAGCAGCT CAACTGCGTG GAGATCTCCG 
     3181 CCCTCAATGG CAACCGCATC AACGTCCCCA TGGTACGAAA GAGGAGCGTT TCCAACTCCA 
     3241 CGCAGACCAG CAGCAGCAGC AGCCAGTCGG AGACCAGCAG CGCGGTCAGC ACGCCCAGCC 
     3301 CTGTTACGAG GACCCGGAGC CTCAGTGCGT GCAACAAGCG GGTGGGCATG TACTTAGAGG 
     3361 GCTTCGATCC TTTCAATCAG TCAACATTTA ACAACGTGGT GGAGCAGAAC TTTAAGAACC 
     3421 GCGAGAGCTA CCCGGAGGAC ACGGTGTTCT ACATCCCTGA AGATCACAAG CCTGGCACTT 
     3481 TCCCCAAAGC TCTCACCAAT GGCAGTTTCT CCCCCTCGGG GAATAACGGC TCTGTGAACT 
     3541 GGAGGACGGG AAGCTTCCAC GGCCCTGGCC ACATCAGCCT CAGGAGGGAA AACAGTAGCG 
     3601 ACAGCCCCAA GGAACTGAAG AGACGCAATT CTTCCAGCTC CATGAGCAGC CGCCTGAGCA 
     3661 TCTACGACAA CGTGCCGGGC TCCATCCTCT ACTCCAGTTC AGGGGACCTG GCGGATCTGG 
     3721 AGAACGAGGA CATCTTCCCC GAGCTGGACG ACATCCTCTA CCACGTGAAG GGGATGCAGC 
     3781 GGATAGTCAA TCAGTGGTCG GAGAAGTTTT CTGATGAGGG AGATTCGGAC TCAGCCCTGG 
     3841 ACTCGGTCTC TCCCTGCCCG TCCTCTCCAA AACAGATACA CCTGGATGTG GACAACGACC 
     3901 GAACCACACC CAGCGACCTG GACAGCACAG GCAACTCCCT GAATGAACCG GAAGAGCCCT 
     3961 CCGAGATCCC GGAAAGAAGG GATTCTGGGG TTGGGGCTTC CCTAACCAGG TCCAACAGGC 
     4021 ACCGACTGAG ATGGCACAGT TTCCAGAGCT CACATCGGCC AAGCCTCAAC TCTGTATCAC 
     4081 TACAGATTAA CTGCCAGTCT GTGGCCCAGA TGAACCTGCT GCAGAAATAC TCACTCCTAA 
     4141 AGCTAACGGC CCTGCTGGAG AAATACACAC CTTCTAACAA GCATGGTTTT AGCTGGGCCG 
     4201 TGCCCAAGTT CATGAAGAGG ATCAAGGTTC CAGACTACAA GGACCGGAGT GTGTTTGGGG 
     4261 TCCCACTGAC GGTCAACGTG CAGCGCACAG GACAACCGTT GCCTCAGAGC ATCCAGCAGG 
     4321 CCATGCGATA CCTCCGGAAC CATTGTTTGG ATCAGGTTGG GCTCTTCAGA AAATCGGGGG 
     4381 TCAAGTCCCG GATTCAGGCT CTGCGCCAGA TGAATGAAGG TGCCATAGAC TGTGTCAACT 
     4441 ACGAAGGACA GTCTGCTTAT GACGTGGCAG ACATGCTGAA GCAGTATTTT CGAGATCTTC 
     4501 CTGAGCCACT AATGACGAAC AAACTCTCGG AAACCTTTCT ACAGATCTAC CAATATGTGC 
     4561 CCAAGGACCA GCGCCTGCAG GCCATCAAGG CTGCCATCAT GCTGCTGCCT GACGAGAACC 
     4621 GGGAGGTTCT GCAGACCCTG CTTTATTTCC TGAGCGATGT CACAGCAGCC GTAAAAGAAA 
     4681 ACCAGATGAC CCCAACCAAC CTGGCCGTGT GCTTAGCGCC TTCCCTCTTC CATCTCAACA 
     4741 CCCTGAAGAG AGAGAATTCC TCTCCCAGGG TAATGCAAAG AAAACAAAGT TTGGGCAAAC 
     4801 CAGATCAGAA AGATTTGAAT GAAAACCTAG CTGCCACTCA AGGGCTGGCC CATATGATCG 
     4861 CCGAGTGCAA GAAGCTTTTC CAGGTTCCCG AGGAAATGAG CCGATGTCGT AATTCCTATA 
     4921 CCGAACAAGA GCTGAAGCCC CTCACTCTGG AAGCACTCGG GCACCTGGGT AATGATGACT 
     4981 CAGCTGACTA CCAACACTTC CTCCAGGACT GTGTGGATGG CCTGTTTAAA GAAGTCAAAG 
     5041 AGAAGTTTAA AGGCTGGGTC AGCTACTCCA CTTCGGAGCA GGCTGAGCTG TCCTATAAGA 
     5101 AGGTGAGCGA AGGACCCCCT CTGAGGCTTT GGAGGTCAGT CATTGAAGTC CCTGCTGTGC 
     5161 CAGAGGAAAT CTTAAAGCGC CTACTTAAAG AACAGCACCT CTGGGATGTA GACCTGTTGG 
     5221 ATTCAAAAGT GATCGAAATT CTGGACAGCC AAACTGAAAT TTACCAGTAT GTCCAAAACA 
     5281 GTATGGCACC TCATCCTGCT CGAGACTACG TTGTTTTAAG AACCTGGAGG ACTAATTTAC 
     5341 CCAAAGGAGC CTGTGCCCTT TTACTAACCT CTGTGGATCA CGATCGCGCA CCTGTGGTGG 
     5401 GTGTGAGGGT TAATGTGCTC TTGTCCAGGT ATTTGATTGA ACCCTGTGGG CCAGGAAAAT 
     5461 CCAAACTCAC CTACATGTGC AGAGTTGACT TAAGGGGCCA CATGCCAGAA TGGTACACAA 
     5521 AATCTTTTGG ACATTTGTGT GCAGCTGAAG TTGTAAAGAT CCGGGATTCC TTCAGTAACC 
     5581 AGAACACTGA AACCAAAGAC ACCAAATCTA GGACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     5641 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     5701 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     5761 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     5821 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5881 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5941 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     6001 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     6061 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     6121 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     6181 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     6241 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     6301 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     6361 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     6421 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     6481 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     6541 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     6601 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     6661 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     6721 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     6781 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6841 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6901 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6961 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     7021 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     7081 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     7141 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     7201 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     7261 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     7321 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     7381 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     7441 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     7501 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     7561 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     7621 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     7681 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     7741 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     7801 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7861 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7921 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7981 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     8041 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     8101 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     8161 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     8221 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     8281 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     8341 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     8401 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     8461 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     8521 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     8581 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     8641 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     8701 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8761 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8821 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8881 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8941 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     9001 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     9061 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     9121 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     9181 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     9241 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     9301 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     9361 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     9421 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//