LOCUS       RC208600                7110 bp    DNA     circular  SYN 
DEFINITION  NAGLU (Myc-DDK-tagged)-Human N-acetylglucosaminidase, alpha (NAGLU)
ACCESSION   NM_000263
VERSION     NM_000263.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3267..3358 
                     /label="Myc-DDK"  
     misc_feature    3388..3973 
                     /label="PolyA signal"  
     misc_feature    3445..3464 
                     /label="Restriction Enzyme"  
     misc_feature    4249..4921 
                     /label="ColE1"  
     misc_feature    5351..6145 
                     /label="Kan/Neo"  
     misc_feature    6180..6482 
                     /label="SV40 ori"  
     misc_feature    6568..7045 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3249..3255 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3249 
                     /label="NAGLU"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGCGGTG GCGGTGGCCG CGGCGGTGGG GGTCCTTCTC CTGGCCGGGG 
     1081 CCGGGGGCGC GGCAGGCGAC GAGGCCCGGG AGGCGGCGGC CGTGCGGGCG CTCGTGGCCC 
     1141 GGCTGCTGGG GCCAGGCCCC GCGGCCGACT TCTCCGTGTC GGTGGAGCGC GCTCTGGCTG 
     1201 CCAAGCCGGG CTTGGACACC TACAGCCTGG GCGGCGGCGG CGCGGCGCGC GTGCGGGTGC 
     1261 GCGGCTCCAC GGGCGTGGCG GCCGCCGCGG GGCTGCACCG CTACCTGCGC GACTTCTGTG 
     1321 GCTGCCACGT GGCCTGGTCC GGCTCTCAGC TGCGCCTGCC GCGGCCACTG CCAGCCGTGC 
     1381 CGGGGGAGCT GACCGAGGCC ACGCCCAACA GGTACCGCTA TTACCAGAAT GTGTGCACGC 
     1441 AAAGCTACTC CTTCGTGTGG TGGGACTGGG CCCGCTGGGA GCGAGAGATA GACTGGATGG 
     1501 CGCTGAATGG CATCAACCTG GCACTGGCCT GGAGCGGCCA GGAGGCCATC TGGCAGCGGG 
     1561 TGTACCTGGC CTTGGGCCTG ACCCAGGCAG AGATCAATGA GTTCTTTACT GGTCCTGCCT 
     1621 TCCTGGCCTG GGGGCGAATG GGCAACCTGC ACACCTGGGA TGGCCCCCTG CCCCCCTCCT 
     1681 GGCACATCAA GCAGCTTTAC CTGCAGCACC GGGTCCTGGA CCAGATGCGC TCCTTCGGCA 
     1741 TGACCCCAGT GCTGCCTGCA TTCGCGGGGC ATGTTCCCGA GGCTGTCACC AGGGTGTTCC 
     1801 CTCAGGTCAA TGTCACGAAG ATGGGCAGTT GGGGCCACTT TAACTGTTCC TACTCCTGCT 
     1861 CCTTCCTTCT GGCTCCGGAA GACCCCATAT TCCCCATCAT CGGGAGCCTC TTCCTGCGAG 
     1921 AGCTGATCAA AGAGTTTGGC ACAGACCACA TCTATGGGGC CGACACTTTC AATGAGATGC 
     1981 AGCCACCTTC CTCAGAGCCC TCCTACCTTG CCGCAGCCAC CACTGCCGTC TATGAGGCCA 
     2041 TGACTGCAGT GGATACTGAG GCTGTGTGGC TGCTCCAAGG CTGGCTCTTC CAGCACCAGC 
     2101 CGCAGTTCTG GGGGCCCGCC CAGATCAGGG CTGTGCTGGG AGCTGTGCCC CGTGGCCGCC 
     2161 TCCTGGTTCT GGACCTGTTT GCTGAGAGCC AGCCTGTGTA TACCCGCACT GCCTCCTTCC 
     2221 AGGGCCAGCC CTTCATCTGG TGCATGCTGC ACAACTTTGG GGGAAACCAT GGTCTTTTTG 
     2281 GAGCCCTAGA GGCTGTGAAC GGAGGCCCAG AAGCTGCCCG CCTCTTCCCC AACTCCACCA 
     2341 TGGTAGGCAC GGGCATGGCC CCCGAGGGCA TCAGCCAGAA CGAAGTGGTC TATTCCCTCA 
     2401 TGGCTGAGCT GGGCTGGCGA AAGGACCCAG TGCCAGATTT GGCAGCCTGG GTGACCAGCT 
     2461 TTGCCGCCCG GCGGTATGGG GTCTCCCACC CGGACGCAGG GGCAGCGTGG AGGCTACTGC 
     2521 TCCGGAGTGT GTACAACTGC TCCGGGGAGG CCTGCAGGGG CCACAATCGT AGCCCGCTGG 
     2581 TCAGGCGGCC GTCCCTACAG ATGAATACCA GCATCTGGTA CAACCGATCT GATGTGTTTG 
     2641 AGGCCTGGCG GCTGCTGCTC ACATCTGCTC CCTCCCTGGC CACCAGCCCC GCCTTCCGCT 
     2701 ACGACCTGCT GGACCTCACT CGGCAGGCAG TGCAGGAGCT GGTCAGCTTG TACTATGAGG 
     2761 AGGCAAGAAG CGCCTACCTG AGCAAGGAGC TGGCCTCCCT GTTGAGGGCT GGAGGCGTCC 
     2821 TGGCCTATGA GCTGCTGCCG GCACTGGACG AGGTGCTGGC TAGTGACAGC CGCTTCTTGC 
     2881 TGGGCAGCTG GCTAGAGCAG GCCCGAGCAG CGGCAGTCAG TGAGGCCGAG GCCGATTTCT 
     2941 ACGAGCAGAA CAGCCGCTAC CAGCTGACCT TGTGGGGGCC AGAAGGCAAC ATCCTGGACT 
     3001 ATGCCAACAA GCAGCTGGCG GGGTTGGTGG CCAACTACTA CACCCCTCGC TGGCGGCTTT 
     3061 TCCTGGAGGC GCTGGTTGAC AGTGTGGCCC AGGGCATCCC TTTCCAACAG CACCAGTTTG 
     3121 ACAAAAATGT CTTCCAACTG GAGCAGGCCT TCGTTCTCAG CAAGCAGAGG TACCCCAGCC 
     3181 AGCCGCGAGG AGACACTGTG GACCTGGCCA AGAAGATCTT CCTCAAATAT TACCCCCGCT 
     3241 GGGTGGCCGG CTCTTGGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     3301 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     3361 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     3421 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     3481 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     3541 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     3601 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     3661 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     3721 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     3781 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     3841 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     3901 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     3961 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     4021 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     4081 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     4141 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     4201 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     4261 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     4321 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     4381 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     4441 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     4501 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     4561 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     4621 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     4681 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     4741 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     4801 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     4861 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     4921 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     4981 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     5041 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     5101 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     5161 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     5221 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     5281 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     5341 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     5401 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     5461 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     5521 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     5581 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     5641 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     5701 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     5761 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     5821 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     5881 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     5941 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     6001 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     6061 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     6121 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     6181 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     6241 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     6301 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     6361 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6421 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6481 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     6541 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     6601 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     6661 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     6721 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     6781 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     6841 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     6901 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     6961 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     7021 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     7081 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//