LOCUS       RC208530                7809 bp    DNA     circular  SYN 
DEFINITION  PARG (Myc-DDK-tagged)-Human poly (ADP-ribose) glycohydrolase (PARG)
ACCESSION   NM_003631
VERSION     NM_003631.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3966..4057 
                     /label="Myc-DDK"  
     misc_feature    4087..4672 
                     /label="PolyA signal"  
     misc_feature    4144..4163 
                     /label="Restriction Enzyme"  
     misc_feature    4948..5620 
                     /label="ColE1"  
     misc_feature    6050..6844 
                     /label="Kan/Neo"  
     misc_feature    6879..7181 
                     /label="SV40 ori"  
     misc_feature    7267..7744 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3948..3954 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3948 
                     /label="PARG"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAATGCGGGC CCCGGCTGTG AACCCTGCAC CAAGCGACCC CGCTGGGGCG 
     1081 CCGCTACAAC TTCGCCGGCT GCTTCGGACG CCCGGAGCTT TCCCAGCAGG CAGAGGCGCG 
     1141 TCCTCGACCC CAAGGACGCT CACGTGCAGT TCAGGGTCCC ACCGTCCTCG CCAGCCTGCG 
     1201 TCCCAGGGCG GGCGGGACAG CACAGAGGCA GCGCCACCTC GCTTGTTTTC AAACAAAAGA 
     1261 CTATTACCAG TTGGATGGAC ACTAAAGGAA TCAAGACAGC GGAATCAGAA AGTTTGGATA 
     1321 GTAAAGAAAA CAACAATACA AGAATAGAAT CCATGATGAG TTCTGTACAA AAAGATAACT 
     1381 TTTACCAACA TAATGTAGAA AAATTAGAAA ATGTTTCTCA GCTAAGTCTT GATAAGTCAC 
     1441 CCACTGAAAA AAGTACACAG TATTTGAACC AGCATCAGAC TGCAGCAATG TGTAAGTGGC 
     1501 AAAATGAAGG GAAACACACG GAGCAGCTTT TGGAAAGTGA ACCTCAAACA GTAACCCTGG 
     1561 TACCAGAGCA GTTTAGTAAT GCTAACATTG ATCGGTCACC TCAAAATGAT GATCACAGTG 
     1621 ACACAGATAG TGAAGAGAAT AGAGACAATC AACAGTTTCT CACAACTGTA AAGCTTGCAA 
     1681 ATGCAAAGCA GACTACAGAA GATGAACAGG CCAGAGAAGC CAAAAGCCAC CAGAAGTGCA 
     1741 GCAAGTCTTG CGATCCTGGG GAAGACTGTG CAAGTTGTCA GCAAGATGAG ATAGATGTGG 
     1801 TGCCAGAGAG TCCATTGTCA GATGTTGGCT CTGAGGATGT TGGTACTGGG CCAAAAAATG 
     1861 ACAACAAATT GACTAGACAA GAAAGTTGCC TAGGAAATTC TCCTCCATTT GAGAAGGAAA 
     1921 GTGAACCCGA GTCACCGATG GATGTGGATA ATTCTAAAAA TAGTTGTCAA GACTCAGAAG 
     1981 CAGATGAGGA GACAAGTCCA GGTTTTGATG AACAAGAAGA TGGTAGTTCC TCCCAAACAG 
     2041 CAAATAAACC TTCAAGGTTC CAAGCAAGAG ACGCTGACAT TGAATTTAGG AAACGGTACT 
     2101 CTACTAAGGG CGGTGAAGTT AGATTACATT TCCAATTTGA AGGAGGAGAG AGTCGCACTG 
     2161 GAATGAATGA TTTAAATGCT AAACTACCTG GAAATATTTC TAGCCTGAAT GTAGAATGCA 
     2221 GAAATTCTAA GCAACATGGA AAAAAGGATT CTAAAATCAC AGATCATTTC ATGAGACTGC 
     2281 CCAAAGCAGA GGACAGAAGA AAAGAACAGT GGGAAACCAA ACATCAAAGA ACAGAAAGGA 
     2341 AGATCCCTAA ATACGTTCCA CCTCACCTTT CTCCAGATAA GAAGTGGCTT GGAACTCCCA 
     2401 TTGAGGAGAT GAGAAGAATG CCTCGGTGTG GGATCCGGCT GCCTCTCTTG AGACCATCTG 
     2461 CCAATCACAC AGTAACTATT CGGGTAGATC TTTTGCGAGC AGGAGAAGTT CCTAAACCTT 
     2521 TTCCAACACA TTATAAAGAT TTGTGGGATA ACAAGCATGT TAAAATGCCT TGTTCAGAAC 
     2581 AAAATTTGTA CCCAGTGGAA GATGAGAATG GTGAGCGAAC TGCGGGGAGC CGGTGGGAGC 
     2641 TCATTCAGAC TGCACTTCTC AACAAATTTA CACGACCCCA AAACTTGAAG GATGCTATTC 
     2701 TGAAATACAA TGTGGCATAT TCTAAGAAAT GGGACTTTAC AGCTTTGATC GATTTCTGGG 
     2761 ATAAGGTACT TGAAGAAGCA GAAGCTCAAC ATTTATATCA GTCCATCTTG CCTGATATGG 
     2821 TGAAAATTGC ACTCTGTCTG CCAAATATTT GCACCCAGCC AATACCACTC CTGAAACAGA 
     2881 AGATGAATCA TTCCATCACA ATGTCGCAGG AACAGATTGC CAGTCTTTTA GCTAATGCTT 
     2941 TCTTCTGCAC ATTTCCACGA CGAAATGCTA AGATGAAATC GGAGTATTCT AGTTACCCAG 
     3001 ACATTAACTT CAATCGATTG TTTGAGGGAC GTTCATCAAG GAAACCGGAG AAACTTAAAA 
     3061 CGCTCTTCTG CTACTTTAGA AGAGTCACAG AGAAAAAACC TACTGGGTTG GTGACATTTA 
     3121 CAAGACAGAG TCTTGAAGAT TTTCCAGAAT GGGAAAGATG TGAAAAACCC TTGACACGAT 
     3181 TGCATGTCAC TTACGAAGGT ACCATAGAAG AAAATGGCCA AGGCATGCTA CAGGTGGATT 
     3241 TTGCAAATCG TTTTGTTGGA GGTGGTGTAA CCAGTGCAGG ACTTGTGCAA GAAGAAATCC 
     3301 GCTTTTTAAT CAATCCTGAG TTGATTATTT CACGGCTCTT CACTGAGGTG CTGGATCACA 
     3361 ATGAATGTCT AATTATCACA GGTACTGAGC AGTACAGTGA ATACACAGGC TATGCTGAGA 
     3421 CATATCGTTG GTCCCGGAGC CACGAAGATG GGAGTGAAAG GGACGACTGG CAGCGGCGCT 
     3481 GCACTGAGAT CGTTGCCATC GATGCTCTTC ACTTCAGACG CTACCTCGAT CAGTTTGTGC 
     3541 CTGAGAAAAT GAGACGCGAG CTGAACAAGG CTTACTGTGG ATTTCTCCGT CCTGGAGTTT 
     3601 CTTCAGAGAA TCTTTCTGCA GTGGCCACAG GAAACTGGGG CTGTGGTGCC TTTGGGGGTG 
     3661 ATGCCAGGTT AAAAGCCTTA ATACAGATAT TGGCAGCTGC TGCAGCTGAG CGAGATGTGG 
     3721 TTTATTTCAC CTTTGGGGAC TCAGAATTGA TGAGAGACAT TTACAGCATG CACATTTTCC 
     3781 TTACTGAAAG GAAACTCACT GTTGGAGATG TGTATAAGCT GTTGCTACGA TACTACAATG 
     3841 AAGAATGCAG AAACTGTTCC ACCCCTGGAC CAGACATCAA GCTTTATCCA TTCATATACC 
     3901 ATGCTGTCGA GTCCTGTGCA GAGACCGCTG ACCATTCAGG GCAAAGGACA GGGACCACGC 
     3961 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4021 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4081 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4141 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4201 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4261 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4321 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4381 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4441 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4501 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4561 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4621 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4681 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     4741 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     4801 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     4861 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     4921 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     4981 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5041 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5101 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5161 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5221 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5281 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5341 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5401 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     5461 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     5521 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     5581 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     5641 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     5701 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     5761 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     5821 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     5881 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     5941 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6001 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6061 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6121 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6181 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6241 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6301 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6361 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6421 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     6481 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     6541 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     6601 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     6661 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     6721 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     6781 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     6841 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     6901 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     6961 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7021 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7081 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7141 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7201 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7261 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7321 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7381 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7441 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     7501 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     7561 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     7621 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     7681 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     7741 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     7801 GCGAATTTT
//