LOCUS       RC208520                8862 bp    DNA     circular  SYN 
DEFINITION  PNPLA6 (Myc-DDK-tagged)-Human patatin-like phospholipase domain containing 6 (PNPLA6), transcript variant 2
ACCESSION   NM_006702
VERSION     NM_006702.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5019..5110 
                     /label="Myc-DDK"  
     misc_feature    5140..5725 
                     /label="PolyA signal"  
     misc_feature    5197..5216 
                     /label="Restriction Enzyme"  
     misc_feature    6001..6673 
                     /label="ColE1"  
     misc_feature    7103..7897 
                     /label="Kan/Neo"  
     misc_feature    7932..8234 
                     /label="SV40 ori"  
     misc_feature    8320..8797 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5001..5007 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5001 
                     /label="PNPLA6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGCTCCG CTGCAAACTG GAATGGTGCT TGGCGTGATG ATCGGGGCCG 
     1081 GAGTGGCGGT GGTGGTCACG GCCGTGCTCA TCCTCCTGGT GGTGCGGAGG CTGCGAGTGC 
     1141 CAAAAACCCC AGCCCCGGAT GGCCCCCGGT ATCGGTTCCG GAAGAGGGAC AAAGTGCTCT 
     1201 TCTATGGCCG GAAGATTATG CGGAAGGTGT CACAATCCAC CTCCTCCCTC GTGGATACCT 
     1261 CTGTCTCCGC CACCTCCCGG CCACGCATGA GGAAGAAACT GAAGATGCTC AACATTGCCA 
     1321 AGAAGATCCT GCGCATCCAG AAAGAGACGC CCACGCTGCA GCGGAAGGAG CCCCCGCCCG 
     1381 CAGTGCTAGA AGCTGACCTG ACCGAGGGCG ACCTGGCTAA CTCCCATCTG CCCTCTGAAG 
     1441 TGCTTTATAT GCTCAAGAAC GTCCGGGTGC TGGGCCACTT CGAGAAGCCA CTCTTCCTGG 
     1501 AGCTCTGCCG CCACATGGTC TTCCAGCGGC TGGGCCAGGG TGACTACGTC TTCCGGCCGG 
     1561 GCCAGCCAGA TGCCAGCATC TACGTGGTGC AGGACGGGCT GCTGGAGCTC TGTCTGCCAG 
     1621 GGCCTGACGG GAAGGAGTGT GTGGTGAAGG AAGTGGTTCC TGGGGACAGC GTCAACAGCC 
     1681 TTCTCAGCAT CCTGGATGTC ATCACCGGTC ACCAGCATCC CCAGCGGACC GTGTCTGCCC 
     1741 GGGCGGCCCG GGACTCCACG GTGCTGCGCC TGCCGGTGGA AGCATTCTCC GCGGTCTTCA 
     1801 CCAAGTACCC GGAGAGCTTG GTGCGGGTCG TGCAGATCAT CATGGTGCGG CTGCAGCGAG 
     1861 TCACCTTCCT GGCACTGCAC AACTACCTGG GTCTGACCAA TGAGCTCTTC AGCCACGAGA 
     1921 TCCAGCCCCT GCGTCTGTTC CCCAGCCCCG GCCTCCCAAC TCGCACCAGC CCTGTGCGGG 
     1981 GCTCCAAGAG AATGGTCAGC ACCTCAGCTA CAGACGAGCC CAGGGAGACC CCAGGGCGGC 
     2041 CACCCGATCC CACCGGGGCC CCGCTGCCTG GACCTACAGG GGACCCTGTG AAGCCCACAT 
     2101 CCCTGGAAAC CCCCTCGGCC CCTCTGCTGA GCCGCTGCGT CTCCATGCCA GGGGACATCT 
     2161 CAGGCTTGCA GGGTGGCCCC CGCTCCGACT TCGACATGGC CTATGAGCGT GGCCGGATCT 
     2221 CCGTGTCCCT GCAGGAAGAG GCCTCCGGGG GGTCCCTGGC AGCCCCCGCT CGGACCCCCA 
     2281 CTCAGGAGCC TCGTGAGCAG CCGGCAGGCG CCTGTGAATA CAGCTACTGT GAGGATGAGT 
     2341 CGGCCACTGG TGGCTGCCCT TTCGGGCCCT ACCAGGGCCG CCAGACCAGC AGCATCTTCG 
     2401 AGGCAGCAAA GCAGGAGCTG GCCAAGCTGA TGCGGATTGA GGACCCCTCC CTCCTGAACA 
     2461 GCAGAGTCTT GCTGCACCAC GCCAAAGCTG GCACCATCAT TGCCCGCCAG GGAGACCAGG 
     2521 ACGTGAGCCT GCACTTCGTG CTCTGGGGCT GCCTGCACGT GTACCAGCGC ATGATCGACA 
     2581 AGGCGGAGGA CGTGTGCCTG TTCGTAGCGC AGCCCGGGGA ACTGGTGGGG CAGCTGGCGG 
     2641 TGCTCACTGG CGAACCTCTC ATCTTCACAC TGCGAGCCCA ACGCGACTGC ACCTTCCTGC 
     2701 GGATCTCCAA GTCCGACTTC TATGAGATCA TGCGCGCACA GCCCAGTGTG GTGCTGAGTG 
     2761 CGGCGCACAC GGTGGCAGCC AGGATGTCGC CCTTCGTGCG CCAGATGGAC TTCGCCATCG 
     2821 ACTGGACTGC AGTGGAGGCG GGACGCGCGC TGTACAGGCA GGGCGACCGC TCCGACTGCA 
     2881 CTTACATCGT GCTCAATGGG CGGCTGCGTA GCGTGATCCA GCGAGGCAGT GGCAAGAAGG 
     2941 AGCTGGTGGG CGAGTACGGC CGCGGCGACC TCATCGGCGT GGTGGAGGCA CTGACCCGGC 
     3001 AGCCGCGAGC CACGACGGTG CACGCGGTGC GCGACACGGA GCTGGCCAAG CTTCCCGAGG 
     3061 GCACCTTGGG TCACATCAAA CGCCGGTACC CGCAGGTCGT GACCCGCCTT ATCCACCTAC 
     3121 TGAGCCAGAA AATTCTAGGG AATTTGCAGC AGCTGCAAGG ACCCTTCCCA GCAGGCTCTG 
     3181 GGTTGGGTGT GCCCCCACAC TCGGAACTCA CCAACCCAGC CAGCAACCTG GCAACTGTGG 
     3241 CAATCCTGCC TGTGTGTGCT GAGGTCCCCA TGGTGGCCTT CACGCTGGAG CTGCAGCACG 
     3301 CCCTGCAGGC CATCGGTCCG ACGCTACTCC TTAACAGTGA CATCATCCGG GCACGCCTGG 
     3361 GGGCCTCCGC ACTGGATAGC ATCCAAGAGT TCCGGCTGTC AGGGTGGCTG GCCCAGCAGG 
     3421 AGGATGCACA CCGTATCGTA CTCTACCAGA CGGACGCCTC GCTGACGCCC TGGACCGTGC 
     3481 GCTGCCTGCG ACAGGCCGAC TGCATCCTCA TTGTGGGCCT GGGGGACCAG GAGCCTACCC 
     3541 TCGGCCAGCT GGAGCAGATG CTGGAGAACA CGGCTGTGCG CGCCCTTAAG CAGCTAGTCC 
     3601 TGCTCCACCG AGAGGAGGGC GCGGGCCCCA CGCGCACCGT GGAGTGGCTA AATATGCGCA 
     3661 GCTGGTGCTC GGGGCACCTG CACCTGCGCT GTCCGCGCCG CCTCTTTTCG CGCCGCAGCC 
     3721 CTGCCAAGCT GCATGAGCTC TACGAGAAGG TTTTCTCCAG GCGCGCGGAC CGGCACAGCG 
     3781 ACTTCTCCCG CTTGGCGAGG GTGCTCACGG GGAACACCAT TGCCCTTGTG CTAGGCGGGG 
     3841 GCGGGGCCAG GGGCTGCTCG CACATCGGAG TACTAAAGGC ATTAGAGGAG GCGGGGGTCC 
     3901 CCGTGGACCT GGTGGGCGGC ACGTCCATTG GCTCTTTCAT CGGAGCGTTG TACGCGGAGG 
     3961 AGCGCAGCGC CAGCCGCACG AAGCAGCGGG CCCGGGAGTG GGCCAAGAGC ATGACTTCGG 
     4021 TGCTGGAACC TGTGTTGGAC CTCACGTACC CAGTCACCTC CATGTTCACT GGGTCTGCCT 
     4081 TTAACCGCAG CATCCATCGG GTCTTCCAGG ATAAGCAGAT TGAGGACCTG TGGCTGCCTT 
     4141 ACTTCAACGT GACCACAGAT ATCACCGCCT CAGCCATGCG AGTCCACAAA GATGGCTCCC 
     4201 TGTGGCGGTA CGTGCGCGCC AGCATGACGC TGTCGGGCTA CCTGCCCCCG CTGTGCGACC 
     4261 CCAAGGACGG GCACCTACTC ATGGATGGCG GCTACATCAA CAATCTGCCA GCGGACATCG 
     4321 CCCGCAGCAT GGGTGCCAAA ACGGTCATCG CCATTGACGT GGGGAGCCAG GATGAGACGG 
     4381 ACCTCAGCAC CTACGGGGAC AGCCTGTCCG GCTGGTGGCT GCTGTGGAAG CGGCTGAATC 
     4441 CCTGGGCTGA CAAGGTAAAG GTTCCAGACA TGGCTGAAAT CCAGTCCCGC CTGGCCTACG 
     4501 TGTCCTGTGT GCGGCAGCTA GAGGTTGTCA AGTCCAGCTC CTACTGCGAG TACCTGCGCC 
     4561 CGCCCATCGA CTGCTTCAAG ACCATGGACT TTGGGAAGTT CGACCAGATC TATGATGTGG 
     4621 GCTACCAGTA CGGGAAGGCG GTGTTTGGAG GCTGGAGCCG TGGCAACGTC ATTGAGAAAA 
     4681 TGCTCACAGA CCGGCGGTCT ACAGACCTTA ATGAGAGCCG CCGTGCAGAC GTGCTTGCCT 
     4741 TCCCAAGCTC TGGCTTCACT GACTTGGCAG AGATTGTGTC CCGGATTGAG CCCCCCACGA 
     4801 GCTATGTCTC TGATGGCTGT GCTGACGGAG AGGAGTCAGA TTGTCTGACA GAGTATGAGG 
     4861 AGGACGCCGG ACCCGACTGC TCGAGGGATG AAGGGGGGTC CCCCGAGGGC GCAAGCCCCA 
     4921 GCACTGCCTC CGAGATGGAG GAGGAGAAGT CGATTCTCCG GCAACGACGC TGTCTGCCCC 
     4981 AGGAGCCGCC CGGCTCAGCC ACAGATGCCA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     5041 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     5101 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     5161 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     5221 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     5281 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     5341 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     5401 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5461 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5521 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5581 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5641 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5701 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5761 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5821 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5881 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5941 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     6001 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     6061 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     6121 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     6181 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     6241 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     6301 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     6361 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6421 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6481 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6541 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6601 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6661 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6721 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6781 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6841 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6901 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6961 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     7021 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     7081 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     7141 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     7201 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     7261 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     7321 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     7381 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7441 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7501 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7561 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7621 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7681 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7741 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7801 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7861 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7921 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7981 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     8041 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     8101 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8161 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8221 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     8281 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     8341 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     8401 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8461 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8521 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8581 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8641 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8701 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8761 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8821 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//