LOCUS       RC208519                8451 bp    DNA     circular  SYN 
DEFINITION  ATN1 (Myc-DDK-tagged)-Human atrophin 1 (ATN1), transcript variant 2
ACCESSION   NM_001940
VERSION     NM_001940.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4608..4699 
                     /label="Myc-DDK"  
     misc_feature    4729..5314 
                     /label="PolyA signal"  
     misc_feature    4786..4805 
                     /label="Restriction Enzyme"  
     misc_feature    5590..6262 
                     /label="ColE1"  
     misc_feature    6692..7486 
                     /label="Kan/Neo"  
     misc_feature    7521..7823 
                     /label="SV40 ori"  
     misc_feature    7909..8386 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4590..4596 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4590 
                     /label="ATN1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGACACGA CAGAATAAAG ACTCGATGTC AATGAGGAGT GGACGGAAGA 
     1081 AAGAGGCCCC TGGGCCCCGG GAAGAACTGA GATCGAGGGG CCGGGCCTCC CCTGGAGGGG 
     1141 TCAGCACGTC CAGCAGTGAT GGCAAAGCTG AGAAGTCCAG GCAGACAGCC AAGAAGGCCC 
     1201 GAGTAGAGGA AGCCTCCACC CCAAAGGTCA ACAAGCAGGG TCGGAGTGAG GAGATCTCAG 
     1261 AGAGTGAAAG TGAGGAGACC AATGCACCAA AAAAGACCAA AACTGAGCAG GAACTCCCTC 
     1321 GGCCACAGTC TCCCTCCGAT CTGGATAGCT TGGACGGGCG GAGCCTTAAT GATGATGGCA 
     1381 GCAGCGACCC TAGGGATATC GACCAGGACA ACCGAAGCAC GTCCCCCAGT ATCTACAGCC 
     1441 CTGGAAGTGT GGAGAATGAC TCTGACTCAT CTTCTGGCCT GTCCCAGGGC CCAGCCCGCC 
     1501 CCTACCACCC ACCTCCACTC TTTCCTCCTT CCCCTCAACC GCCAGACAGC ACCCCTCGAC 
     1561 AGCCAGAGGC TAGCTTTGAA CCCCATCCTT CTGTGACACC CACTGGATAT CATGCTCCCA 
     1621 TGGAGCCCCC CACATCTCGA ATGTTCCAGG CTCCTCCTGG GGCCCCTCCC CCTCACCCAC 
     1681 AGCTCTATCC TGGGGGCACT GGTGGAGTTT TGTCTGGACC CCCAATGGGT CCCAAGGGGG 
     1741 GAGGGGCTGC CTCATCAGTG GGGGGCCCTA ATGGGGGTAA GCAGCACCCC CCACCCACTA 
     1801 CTCCCATTTC AGTATCAAGC TCTGGGGCTA GTGGTGCTCC CCCAACAAAG CCGCCTACCA 
     1861 CTCCAGTGGG TGGTGGGAAC CTACCTTCTG CTCCACCACC AGCCAACTTC CCCCATGTGA 
     1921 CACCGAACCT GCCTCCCCCA CCTGCCCTGA GACCCCTCAA CAATGCATCA GCCTCTCCCC 
     1981 CTGGCCTGGG GGCCCAACCA CTACCTGGTC ATCTGCCCTC TCCCCACGCC ATGGGACAGG 
     2041 GTATGGGTGG ACTTCCTCCT GGCCCAGAGA AGGGCCCAAC TCTGGCTCCT TCACCCCACT 
     2101 CTCTGCCTCC TGCTTCCTCT TCTGCTCCAG CGCCCCCCAT GAGGTTTCCT TATTCATCCT 
     2161 CTAGTAGTAG CTCTGCAGCA GCCTCCTCTT CCAGTTCTTC CTCCTCTTCC TCTGCCTCCC 
     2221 CCTTCCCAGC TTCCCAGGCA TTGCCCAGCT ACCCCCACTC TTTCCCTCCC CCAACAAGCC 
     2281 TCTCTGTCTC CAATCAGCCC CCCAAGTATA CTCAGCCTTC TCTCCCATCC CAGGCTGTGT 
     2341 GGAGCCAGGG TCCCCCACCA CCTCCTCCCT ATGGCCGCCT CTTAGCCAAC AGCAATGCCC 
     2401 ATCCAGGCCC CTTCCCTCCC TCTACTGGGG CCCAGTCCAC CGCCCACCCA CCAGTCTCAA 
     2461 CACATCACCA TCACCACCAG CAACAGCAAC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC 
     2521 AGCAGCAGCA GCAGCATCAC GGAAACTCTG GGCCCCCTCC TCCTGGAGCA TTTCCCCACC 
     2581 CACTGGAGGG CGGTAGCTCC CACCACGCAC ACCCTTACGC CATGTCTCCC TCCCTGGGGT 
     2641 CTCTGAGGCC CTACCCACCA GGGCCAGCAC ACCTGCCCCC ACCTCACAGC CAGGTGTCCT 
     2701 ACAGCCAAGC AGGCCCCAAT GGCCCTCCAG TCTCTTCCTC TTCCAACTCT TCCTCTTCCA 
     2761 CTTCTCAAGG GTCCTACCCA TGTTCACACC CCTCCCCTTC CCAGGGCCCT CAAGGGGCGC 
     2821 CCTACCCTTT CCCACCGGTG CCTACGGTCA CCACCTCTTC GGCTACCCTT TCCACGGTCA 
     2881 TTGCCACCGT GGCTTCCTCG CCAGCAGGCT ACAAAACGGC CTCCCCACCT GGGCCCCCAC 
     2941 CGTACGGAAA GAGAGCCCCG TCCCCGGGGG CCTACAAGAC AGCCACCCCA CCCGGATACA 
     3001 AACCCGGGTC GCCTCCCTCC TTCCGAACGG GGACCCCACC GGGCTATCGA GGAACCTCGC 
     3061 CACCTGCAGG CCCAGGGACC TTCAAGCCGG GCTCGCCCAC CGTGGGACCT GGGCCCCTGC 
     3121 CACCTGCGGG GCCCTCAGGC CTGCCATCGC TGCCACCACC ACCTGCGGCC CCTGCCTCAG 
     3181 GGCCGCCCCT GAGCGCCACG CAGATCAAAC AGGAGCCGGC TGAGGAGTAT GAGACCCCCG 
     3241 AGAGCCCGGT GCCCCCAGCC CGCAGCCCCT CGCCCCCTCC CAAGGTGGTA GATGTACCCA 
     3301 GCCATGCCAG TCAGTCTGCC AGGTTCAACA AACACCTGGA TCGCGGCTTC AACTCGTGCG 
     3361 CGCGCAGCGA CCTGTACTTC GTGCCACTGG AGGGCTCCAA GCTGGCCAAG AAGCGGGCCG 
     3421 ACCTGGTGGA GAAGGTGCGG CGCGAGGCCG AGCAGCGCGC GCGCGAAGAA AAGGAGCGCG 
     3481 AGCGCGAGCG GGAACGCGAG AAAGAGCGCG AGCGCGAGAA GGAGCGCGAG CTTGAACGCA 
     3541 GCGTGAAGTT GGCTCAGGAG GGCCGTGCTC CGGTGGAATG CCCATCTCTG GGCCCAGTGC 
     3601 CCCATCGCCC TCCATTTGAA CCGGGCAGTG CGGTGGCTAC AGTGCCCCCC TACCTGGGTC 
     3661 CTGACACTCC AGCCTTGCGC ACTCTCAGTG AATATGCCCG GCCTCATGTC ATGTCTCCTG 
     3721 GCAATCGCAA CCATCCATTC TACGTGCCCC TGGGGGCAGT GGACCCGGGG CTCCTGGGTT 
     3781 ACAATGTCCC GGCCCTGTAC AGCAGTGATC CAGCTGCCCG GGAGAGGGAA CGGGAAGCCC 
     3841 GTGAACGAGA CCTCCGTGAC CGCCTCAAGC CTGGCTTTGA GGTGAAGCCT AGTGAGCTGG 
     3901 AACCCCTACA TGGGGTCCCT GGGCCGGGCT TGGATCCCTT TCCCCGACAT GGGGGCCTGG 
     3961 CTCTGCAGCC TGGCCCACCT GGCCTGCACC CTTTCCCCTT TCATCCGAGC CTGGGGCCCC 
     4021 TGGAGCGAGA ACGTCTAGCG CTGGCAGCTG GGCCAGCCCT GCGGCCTGAC ATGTCCTATG 
     4081 CTGAGCGGCT GGCAGCTGAG AGGCAGCACG CAGAAAGGGT GGCGGCCCTG GGCAATGACC 
     4141 CACTGGCCCG GCTGCAGATG CTCAATGTGA CTCCCCATCA CCACCAGCAC TCCCACATCC 
     4201 ACTCGCACCT GCACCTGCAC CAGCAAGATG CTATCCATGC AGCCTCTGCC TCGGTGCACC 
     4261 CTCTCATTGA CCCCCTGGCC TCAGGGTCTC ACCTTACCCG GATCCCCTAC CCAGCTGGAA 
     4321 CTCTCCCTAA CCCCCTGCTT CCTCACCCTC TGCACGAGAA CGAAGTTCTT CGTCACCAGC 
     4381 TCTTTGCTGC CCCTTACCGG GACCTGCCGG CCTCCCTTTC TGCCCCGATG TCAGCAGCTC 
     4441 ATCAGCTGCA GGCCATGCAC GCACAGTCAG CTGAGCTGCA GCGCTTGGCG CTGGAACAGC 
     4501 AGCAGTGGCT GCATGCCCAT CACCCGCTGC ACAGTGTGCC GCTGCCTGCC CAGGAGGACT 
     4561 ACTACAGTCA CCTGAAGAAG GAAAGCGACA AGCCACTGAC GCGTACGCGG CCGCTCGAGC 
     4621 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4681 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4741 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4801 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4861 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4921 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4981 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5041 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5101 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5161 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5221 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5281 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5341 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5401 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5461 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5521 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5581 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5641 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5701 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5761 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5821 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5881 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5941 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6001 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6061 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6121 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6181 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6241 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6301 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6361 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6421 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6481 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6541 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6601 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6661 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6721 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6781 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6841 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6901 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6961 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7021 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7081 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7141 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7201 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7261 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7321 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7381 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7441 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7501 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7561 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7621 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7681 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7741 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7801 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7861 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7921 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7981 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8041 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8101 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8161 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8221 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8281 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8341 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8401 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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