LOCUS       RC208497                9006 bp    DNA     circular  SYN 
DEFINITION  NLRP1 (Myc-DDK-tagged)-Human NLR family, pyrin domain containing 1 (NLRP1), transcript variant 5
ACCESSION   NM_001033053
VERSION     NM_001033053.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5163..5254 
                     /label="Myc-DDK"  
     misc_feature    5284..5869 
                     /label="PolyA signal"  
     misc_feature    5341..5360 
                     /label="Restriction Enzyme"  
     misc_feature    6145..6817 
                     /label="ColE1"  
     misc_feature    7247..8041 
                     /label="Kan/Neo"  
     misc_feature    8076..8378 
                     /label="SV40 ori"  
     misc_feature    8464..8941 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5145..5151 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5145 
                     /label="NLRP1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTGGCGGA GCCTGGGGCC GCCTGGCCTG TTACTTGGAG TTCCTGAAGA 
     1081 AGGAGGAGCT GAAGGAGTTC CAGCTTCTGC TCGCCAATAA AGCGCACTCC AGGAGCTCTT 
     1141 CGGGTGAGAC ACCCGCTCAG CCAGAGAAGA CGAGTGGCAT GGAGGTGGCC TCGTACCTGG 
     1201 TGGCTCAGTA TGGGGAGCAG CGGGCCTGGG ACCTAGCCCT CCATACCTGG GAGCAGATGG 
     1261 GGCTGAGGTC ACTGTGCGCC CAAGCCCAGG AAGGGGCAGG CCACTCTCCC TCATTCCCCT 
     1321 ACAGCCCAAG TGAACCCCAC CTGGGGTCTC CCAGCCAACC CACCTCCACC GCAGTGCTAA 
     1381 TGCCCTGGAT CCATGAATTG CCGGCGGGGT GCACCCAGGG CTCAGAGAGA AGGGTTTTGA 
     1441 GACAGCTGCC TGACACATCT GGACGCCGCT GGAGAGAAAT CTCTGCCTCA CTCCTCTACC 
     1501 AAGCTCTTCC AAGCTCCCCA GACCATGAGT CTCCAAGCCA GGAGTCACCC AACGCCCCCA 
     1561 CATCCACAGC AGTGCTGGGG AGCTGGGGAT CCCCACCTCA GCCCAGCCTA GCACCCAGAG 
     1621 AGCAGGAGGC TCCTGGGACC CAATGGCCTC TGGATGAAAC GTCAGGAATT TACTACACAG 
     1681 AAATCAGAGA AAGAGAGAGA GAGAAATCAG AGAAAGGCAG GCCCCCATGG GCAGCGGTGG 
     1741 TAGGAACGCC CCCACAGGCG CACACCAGCC TACAGCCCCA CCACCACCCA TGGGAGCCTT 
     1801 CTGTGAGAGA GAGCCTCTGT TCCACATGGC CCTGGAAAAA TGAGGATTTT AACCAAAAAT 
     1861 TCACACAGCT GCTACTTCTA CAAAGACCTC ACCCCAGAAG CCAAGATCCC CTGGTCAAGA 
     1921 GAAGCTGGCC TGATTATGTG GAGGAGAATC GAGGACATTT AATTGAGATC AGAGACTTAT 
     1981 TTGGCCCAGG CCTGGATACC CAAGAACCTC GCATAGTCAT ACTGCAGGGG GCTGCTGGAA 
     2041 TTGGGAAGTC AACACTGGCC AGGCAGGTGA AGGAAGCCTG GGGGAGAGGC CAGCTGTATG 
     2101 GGGACCGCTT CCAGCATGTC TTCTACTTCA GCTGCAGAGA GCTGGCCCAG TCCAAGGTGG 
     2161 TGAGTCTCGC TGAGCTCATC GGAAAAGATG GGACAGCCAC TCCGGCTCCC ATTAGACAGA 
     2221 TCCTGTCTAG GCCAGAGCGG CTGCTCTTCA TCCTCGATGG TGTAGATGAG CCAGGATGGG 
     2281 TCTTGCAGGA GCCGAGTTCT GAGCTCTGTC TGCACTGGAG CCAGCCACAG CCGGCGGATG 
     2341 CACTGCTGGG CAGTTTGCTG GGGAAAACTA TACTTCCCGA GGCATCCTTC CTGATCACGG 
     2401 CTCGGACCAC AGCTCTGCAG AACCTCATTC CTTCTTTGGA GCAGGCACGT TGGGTAGAGG 
     2461 TCCTGGGGTT CTCTGAGTCC AGCAGGAAGG AATATTTCTA CAGATATTTC ACAGATGAAA 
     2521 GGCAAGCAAT TAGAGCCTTT AGGTTGGTCA AATCAAACAA AGAGCTCTGG GCCCTGTGTC 
     2581 TTGTGCCCTG GGTGTCCTGG CTGGCCTGCA CTTGCCTGAT GCAGCAGATG AAGCGGAAGG 
     2641 AAAAACTCAC ACTGACTTCC AAGACCACCA CAACCCTCTG TCTACATTAC CTTGCCCAGG 
     2701 CTCTCCAAGC TCAGCCATTG GGACCCCAGC TCAGAGACCT CTGCTCTCTG GCTGCTGAGG 
     2761 GCATCTGGCA AAAAAAGACC CTTTTCAGTC CAGATGACCT CAGGAAGCAT GGGTTAGATG 
     2821 GGGCCATCAT CTCCACCTTC TTGAAGATGG GTATTCTTCA AGAGCACCCC ATCCCTCTGA 
     2881 GCTACAGCTT CATTCACCTC TGTTTCCAAG AGTTCTTTGC AGCAATGTCC TATGTCTTGG 
     2941 AGGATGAGAA GGGGAGAGGT AAACATTCTA ATTGCATCAT AGATTTGGAA AAGACGCTAG 
     3001 AAGCATATGG AATACATGGC CTGTTTGGGG CATCAACCAC ACGTTTCCTA TTGGGCCTGT 
     3061 TAAGTGATGA GGGGGAGAGA GAGATGGAGA ACATCTTTCA CTGCCGGCTG TCTCAGGGGA 
     3121 GGAACCTGAT GCAGTGGGTC CCGTCCCTGC AGCTGCTGCT GCAGCCACAC TCTCTGGAGT 
     3181 CCCTCCACTG CTTGTACGAG ACTCGGAACA AAACGTTCCT GACACAAGTG ATGGCCCATT 
     3241 TCGAAGAAAT GGGCATGTGT GTAGAAACAG ACATGGAGCT CTTAGTGTGC ACTTTCTGCA 
     3301 TTAAATTCAG CCGCCACGTG AAGAAGCTTC AGCTGATTGA GGGCAGGCAG CACAGATCAA 
     3361 CATGGAGCCC CACCATGGTA GTCCTGTTCA GGTGGGTCCC AGTCACAGAT GCCTATTGGC 
     3421 AGATTCTCTT CTCCGTCCTC AAGGTCACCA GAAACCTGAA GGAGCTGGAC CTAAGTGGAA 
     3481 ACTCGCTGAG CCACTCTGCA GTGAAGAGTC TTTGTAAGAC CCTGAGACGC CCTCGCTGCC 
     3541 TCCTGGAGAC CCTGCGGTTG GCTGGCTGTG GCCTCACAGC TGAGGACTGC AAGGACCTTG 
     3601 CCTTTGGGCT GAGAGCCAAC CAGACCCTGA CCGAGCTGGA CCTGAGCTTC AATGTGCTCA 
     3661 CGGATGCTGG AGCCAAACAC CTTTGCCAGA GACTGAGACA GCCGAGCTGC AAGCTACAGC 
     3721 GACTGCAGCT GGTCAGCTGT GGCCTCACGT CTGACTGCTG CCAGGACCTG GCCTCTGTGC 
     3781 TTAGTGCCAG CCCCAGCCTG AAGGAGCTAG ACCTGCAGCA GAACAACCTG GATGACGTTG 
     3841 GCGTGCGACT GCTCTGTGAG GGGCTCAGGC ATCCTGCCTG CAAACTCATA CGCCTGGGGC 
     3901 TGGACCAGAC AACTCTGAGT GATGAGATGA GGCAGGAACT GAGGGCCCTG GAGCAGGAGA 
     3961 AACCTCAGCT GCTCATCTTC AGCAGACGGA AACCAAGTGT GATGACCCCT ACTGAGGGCC 
     4021 TGGATACGGG AGAGATGAGT AATAGCACAT CCTCACTCAA GCGGCAGAGA CTCGGATCAG 
     4081 AGAGGGCGGC TTCCCATGTT GCTCAGGCTA ATCTCAAACT CCTGGACGTG AGCAAGATCT 
     4141 TCCCAATTGC TGAGATTGCA GGCAAGAGCC ACGAGGAAAG CTCCCCAGAG GTAGTACCGG 
     4201 TGGAACTCTT GTGCGTGCCT TCTCCTGCCT CTCAAGGGGA CCTGCATACG AAGCCTTTGG 
     4261 GGACTGACGA TGACTTCTGG GGCCCCACGG GGCCTGTGGC TACTGAGGTA GTTGACAAAG 
     4321 AAAAGAACTT GTACCGAGTT CACTTCCCTG TAGCTGGCTC CTACCGCTGG CCCAACACGG 
     4381 GTCTCTGCTT TGTGATGAGA GAAGCGGTGA CCGTTGAGAT TGAATTCTGT GTGTGGGACC 
     4441 AGTTCCTGGG TGAGATCAAC CCACAGCACA GCTGGATGGT GGCAGGGCCT CTGCTGGACA 
     4501 TCAAGGCTGA GCCTGGAGCT GTGGAAGCTG TGCACCTCCC TCACTTTGTG GCTCTCCAAG 
     4561 GGGGCCATGT GGACACATCC CTGTTCCAAA TGGCCCACTT TAAAGAGGAG GGGATGCTCC 
     4621 TGGAGAAGCC AGCCAGGGTG GAGCTGCATC ACATAGTTCT GGAAAACCCC AGCTTCTCCC 
     4681 CCTTGGGAGT CCTCCTGAAA ATGATCCATA ATGCCCTGCG CTTCATTCCC GTCACCTCTG 
     4741 TGGTGTTGCT TTACCACCGC GTCCATCCTG AGGAAGTCAC CTTCCACCTC TACCTGATCC 
     4801 CAAGTGACTG CTCCATTCGG AAGGCCATAG ATGATCTAGA AATGAAATTC CAGTTTGTGC 
     4861 GAATCCACAA GCCACCCCCG CTGACCCCAC TTTATATGGG CTGTCGTTAC ACTGTGTCTG 
     4921 GGTCTGGTTC AGGGATGCTG GAAATACTCC CCAAGGAACT GGAGCTCTGC TATCGAAGCC 
     4981 CTGGAGAAGA CCAGCTGTTC TCGGAGTTCT ACGTTGGCCA CTTGGGATCA GGGATCAGGC 
     5041 TGCAAGTGAA AGACAAGAAA GATGAGACTC TGGTGTGGGA GGCCTTGGTG AAACCAGGAA 
     5101 GGAACACCAG CCAGCCGTGG AACCTCAGGT GCAACAGAGA CGCCAGGAGA TACACGCGTA 
     5161 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     5221 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     5281 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     5341 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     5401 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     5461 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5521 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5581 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5641 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5701 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5761 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5821 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5881 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5941 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     6001 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     6061 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     6121 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     6181 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     6241 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     6301 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     6361 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     6421 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6481 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6541 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6601 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6661 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6721 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6781 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6841 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6901 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6961 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     7021 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     7081 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     7141 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     7201 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     7261 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     7321 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     7381 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     7441 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7501 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7561 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7621 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7681 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7741 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7801 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7861 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7921 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7981 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     8041 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     8101 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     8161 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     8221 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     8281 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     8341 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8401 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     8461 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8521 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8581 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8641 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8701 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8761 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8821 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8881 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8941 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     9001 AATTTT
//