LOCUS       RC208484                8979 bp    DNA     circular  SYN 
DEFINITION  COL1A2 (Myc-DDK-tagged)-Human collagen, type I, alpha 2 (COL1A2)
ACCESSION   NM_000089
VERSION     NM_000089.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5136..5227 
                     /label="Myc-DDK"  
     misc_feature    5257..5842 
                     /label="PolyA signal"  
     misc_feature    5314..5333 
                     /label="Restriction Enzyme"  
     misc_feature    6118..6790 
                     /label="ColE1"  
     misc_feature    7220..8014 
                     /label="Kan/Neo"  
     misc_feature    8049..8351 
                     /label="SV40 ori"  
     misc_feature    8437..8914 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5118..5124 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5118 
                     /label="COL1A2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTCAGCTTT GTGGATACGC GGACTTTGTT GCTGCTTGCA GTAACCTTAT 
     1081 GCCTAGCAAC ATGCCAATCT TTACAAGAGG AAACTGTAAG AAAGGGCCCA GCCGGAGATA 
     1141 GAGGACCACG TGGAGAAAGG GGTCCACCAG GCCCCCCAGG CAGAGATGGT GAAGATGGTC 
     1201 CCACAGGCCC TCCTGGTCCA CCTGGTCCTC CTGGCCCCCC TGGTCTCGGT GGGAACTTTG 
     1261 CTGCTCAGTA TGATGGAAAA GGAGTTGGAC TTGGCCCTGG ACCAATGGGC TTAATGGGAC 
     1321 CTAGAGGCCC ACCTGGTGCA GCTGGAGCCC CAGGCCCTCA AGGTTTCCAA GGACCTGCTG 
     1381 GTGAGCCTGG TGAACCTGGT CAAACTGGTC CTGCAGGTGC TCGTGGTCCA GCTGGCCCTC 
     1441 CTGGCAAGGC TGGTGAAGAT GGTCACCCTG GAAAACCCGG ACGACCTGGT GAGAGAGGAG 
     1501 TTGTTGGACC ACAGGGTGCT CGTGGTTTCC CTGGAACTCC TGGACTTCCT GGCTTCAAAG 
     1561 GCATTAGGGG ACACAATGGT CTGGATGGAT TGAAGGGACA GCCCGGTGCT CCTGGTGTGA 
     1621 AGGGTGAACC TGGTGCCCCT GGTGAAAATG GAACTCCAGG TCAAACAGGA GCCCGTGGGC 
     1681 TTCCTGGTGA GAGAGGACGT GTTGGTGCCC CTGGCCCAGC TGGTGCCCGT GGCAGTGATG 
     1741 GAAGTGTGGG TCCCGTGGGT CCTGCTGGTC CCATTGGGTC TGCTGGCCCT CCAGGCTTCC 
     1801 CAGGTGCCCC TGGCCCCAAG GGTGAAATTG GAGCTGTTGG TAACGCTGGT CCTGCTGGTC 
     1861 CCGCCGGTCC CCGTGGTGAA GTGGGTCTTC CAGGCCTCTC CGGCCCCGTT GGACCTCCTG 
     1921 GTAATCCTGG AGCAAACGGC CTTACTGGTG CCAAGGGTGC TGCTGGCCTT CCCGGCGTTG 
     1981 CTGGGGCTCC CGGCCTCCCT GGACCCCGCG GTATTCCTGG CCCTGTTGGT GCTGCCGGTG 
     2041 CTACTGGTGC CAGAGGACTT GTTGGTGAGC CTGGTCCAGC TGGCTCCAAA GGAGAGAGCG 
     2101 GTAACAAGGG TGAGCCCGGC TCTGCTGGGC CCCAAGGTCC TCCTGGTCCC AGTGGTGAAG 
     2161 AAGGAAAGAG AGGCCCTAAT GGGGAAGCTG GATCTGCCGG CCCTCCAGGA CCTCCTGGGC 
     2221 TGAGAGGTAG TCCTGGTTCT CGTGGTCTTC CTGGAGCTGA TGGCAGAGCT GGCGTCATGG 
     2281 GCCCTCCTGG TAGTCGTGGT GCAAGTGGCC CTGCTGGAGT CCGAGGACCT AATGGAGATG 
     2341 CTGGTCGCCC TGGGGAGCCT GGTCTCATGG GACCCAGAGG TCTTCCTGGT TCCCCTGGAA 
     2401 ATATCGGCCC CGCTGGAAAA GAAGGTCCTG TCGGCCTCCC TGGCATCGAC GGCAGGCCTG 
     2461 GCCCAATTGG CCCAGCTGGA GCAAGAGGAG AGCCTGGCAA CATTGGATTC CCTGGACCCA 
     2521 AAGGCCCCAC TGGTGATCCT GGCAAAAACG GTGATAAAGG TCATGCTGGT CTTGCTGGTG 
     2581 CTCGGGGTGC TCCAGGTCCT GATGGAAACA ATGGTGCTCA GGGACCTCCT GGACCACAGG 
     2641 GTGTTCAAGG TGGAAAAGGT GAACAGGGTC CCCCTGGTCC TCCAGGCTTC CAGGGTCTGC 
     2701 CTGGCCCCTC AGGTCCCGCT GGTGAAGTTG GCAAACCAGG AGAAAGGGGT CTCCATGGTG 
     2761 AGTTTGGTCT CCCTGGTCCT GCTGGTCCAA GAGGGGAACG CGGTCCCCCA GGTGAGAGTG 
     2821 GTGCTGCCGG TCCTACTGGT CCTATTGGAA GCCGAGGTCC TTCTGGACCC CCAGGGCCTG 
     2881 ATGGAAACAA GGGTGAACCT GGTGTGGTTG GTGCTGTGGG CACTGCTGGT CCATCTGGTC 
     2941 CTAGTGGACT CCCAGGAGAG AGGGGTGCTG CTGGCATACC TGGAGGCAAG GGAGAAAAGG 
     3001 GTGAACCTGG TCTCAGAGGT GAAATTGGTA ACCCTGGCAG AGATGGTGCT CGTGGTGCTC 
     3061 CTGGTGCTGT AGGTGCCCCT GGTCCTGCTG GAGCCACAGG TGACCGGGGC GAAGCTGGGG 
     3121 CTGCTGGTCC TGCTGGTCCT GCTGGTCCTC GGGGAAGCCC TGGTGAACGT GGTGAGGTCG 
     3181 GTCCTGCTGG CCCCAATGGA TTTGCTGGTC CTGCTGGTGC TGCTGGTCAA CCTGGTGCTA 
     3241 AAGGAGAAAG AGGAGCCAAA GGGCCTAAGG GTGAAAACGG TGTTGTTGGT CCCACAGGCC 
     3301 CCGTTGGAGC TGCTGGCCCA GCTGGTCCAA ATGGTCCCCC CGGTCCTGCT GGAAGTCGTG 
     3361 GTGATGGAGG CCCCCCTGGT ATGACTGGTT TCCCTGGTGC TGCTGGACGG ACTGGTCCCC 
     3421 CAGGACCCTC TGGTATTTCT GGCCCTCCTG GTCCCCCTGG TCCTGCTGGG AAAGAAGGGC 
     3481 TTCGTGGTCC TCGTGGTGAC CAAGGTCCAG TTGGCCGAAC TGGAGAAGTA GGTGCAGTTG 
     3541 GTCCCCCTGG CTTCGCTGGT GAGAAGGGTC CCTCTGGAGA GGCTGGTACT GCTGGACCTC 
     3601 CTGGCACTCC AGGTCCTCAG GGTCTTCTTG GTGCTCCTGG TATTCTGGGT CTCCCTGGCT 
     3661 CGAGAGGTGA ACGTGGTCTA CCAGGTGTTG CTGGTGCTGT GGGTGAACCT GGTCCTCTTG 
     3721 GCATTGCCGG CCCTCCTGGG GCCCGTGGTC CTCCTGGTGC TGTGGGTAGT CCTGGAGTCA 
     3781 ACGGTGCTCC TGGTGAAGCT GGTCGTGATG GCAACCCTGG GAACGATGGT CCCCCAGGTC 
     3841 GCGATGGTCA ACCCGGACAC AAGGGAGAGC GCGGTTACCC TGGCAATATT GGTCCCGTTG 
     3901 GTGCTGCAGG TGCACCTGGT CCTCATGGCC CCGTGGGTCC TGCTGGCAAA CATGGAAACC 
     3961 GTGGTGAAAC TGGTCCTTCT GGTCCTGTTG GTCCTGCTGG TGCTGTTGGC CCAAGAGGTC 
     4021 CTAGTGGCCC ACAAGGCATT CGTGGCGATA AGGGAGAGCC CGGTGAAAAG GGGCCCAGAG 
     4081 GTCTTCCTGG CTTAAAGGGA CACAATGGAT TGCAAGGTCT GCCTGGTATC GCTGGTCACC 
     4141 ATGGTGATCA AGGTGCTCCT GGCTCCGTGG GTCCTGCTGG TCCTAGGGGC CCTGCTGGTC 
     4201 CTTCTGGCCC TGCTGGAAAA GATGGTCGCA CTGGACATCC TGGTACAGTT GGACCTGCTG 
     4261 GCATTCGAGG CCCTCAGGGT CACCAAGGCC CTGCTGGCCC CCCTGGTCCC CCTGGCCCTC 
     4321 CTGGACCTCC AGGTGTAAGC GGTGGTGGTT ATGACTTTGG TTACGATGGA GACTTCTACA 
     4381 GGGCTGACCA GCCTCGCTCA GCACCTTCTC TCAGACCCAA GGACTATGAA GTTGATGCTA 
     4441 CTCTGAAGTC TCTCAACAAC CAGATTGAGA CCCTTCTTAC TCCTGAAGGC TCTAGAAAGA 
     4501 ACCCAGCTCG CACATGCCGT GACTTGAGAC TCAGCCACCC AGAGTGGAGC AGTGGTTACT 
     4561 ACTGGATTGA CCCTAACCAA GGATGCACTA TGGATGCTAT CAAAGTATAC TGTGATTTCT 
     4621 CTACTGGCGA AACCTGTATC CGGGCCCAAC CTGAAAACAT CCCAGCCAAG AACTGGTATA 
     4681 GGAGCTCCAA GGACAAGAAA CACGTCTGGC TAGGAGAAAC TATCAATGCT GGCAGCCAGT 
     4741 TTGAATATAA TGTAGAAGGA GTGACTTCCA AGGAAATGGC TACCCAACTT GCCTTCATGC 
     4801 GCCTGCTGGC CAACTATGCC TCTCAGAACA TCACCTACCA CTGCAAGAAC AGCATTGCAT 
     4861 ACATGGATGA GGAGACTGGC AACCTGAAAA AGGCTGTCAT TCTACAGGGC TCTAATGATG 
     4921 TTGAACTTGT TGCTGAGGGC AACAGCAGGT TCACTTACAC TGTTCTTGTA GATGGCTGCT 
     4981 CTAAAAAGAC AAATGAATGG GGAAAGACAA TCATTGAATA CAAAACAAAT AAGCCATCAC 
     5041 GCCTGCCCTT CCTTGATATT GCACCTTTGG ACATCGGTGG TGCTGACCAG GAATTCTTTG 
     5101 TGGACATTGG CCCAGTCTGT TTCAAAACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     5161 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     5221 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     5281 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     5341 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     5401 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     5461 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     5521 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     5581 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5641 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5701 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5761 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5821 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5881 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5941 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     6001 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     6061 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     6121 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     6181 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     6241 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     6301 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     6361 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     6421 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     6481 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     6541 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6601 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6661 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6721 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6781 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6841 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6901 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6961 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     7021 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     7081 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     7141 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     7201 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     7261 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     7321 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     7381 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     7441 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     7501 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     7561 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7621 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7681 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7741 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7801 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7861 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7921 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7981 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     8041 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     8101 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     8161 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     8221 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8281 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8341 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     8401 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     8461 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     8521 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     8581 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     8641 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8701 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8761 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8821 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8881 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8941 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//