LOCUS       RC208364                7875 bp    DNA     circular  SYN 
DEFINITION  EPHB3 (Myc-DDK-tagged)-Human EPH receptor B3 (EPHB3)
ACCESSION   NM_004443
VERSION     NM_004443.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4032..4123 
                     /label="Myc-DDK"  
     misc_feature    4153..4738 
                     /label="PolyA signal"  
     misc_feature    4210..4229 
                     /label="Restriction Enzyme"  
     misc_feature    5014..5686 
                     /label="ColE1"  
     misc_feature    6116..6910 
                     /label="Kan/Neo"  
     misc_feature    6945..7247 
                     /label="SV40 ori"  
     misc_feature    7333..7810 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4014..4020 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4014 
                     /label="EPHB3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCAGAGCC CGCCCGCCGC CGCCGCCGTC GCCGCCGCCG GGGCTTCTGC 
     1081 CGCTGCTCCC TCCGCTGCTG CTGCTGCCGC TGCTGCTGCT GCCCGCCGGC TGCCGGGCGC 
     1141 TGGAAGAGAC CCTCATGGAC ACAAAATGGG TAACATCTGA GTTGGCGTGG ACATCTCATC 
     1201 CAGAAAGTGG GTGGGAAGAG GTGAGTGGCT ACGATGAGGC CATGAATCCC ATCCGCACAT 
     1261 ACCAGGTGTG TAATGTGCGC GAGTCAAGCC AGAACAACTG GCTTCGCACG GGGTTCATCT 
     1321 GGCGGCGGGA TGTGCAGCGG GTCTACGTGG AGCTCAAGTT CACTGTGCGT GACTGCAACA 
     1381 GCATCCCCAA CATCCCCGGC TCCTGCAAGG AGACCTTCAA CCTCTTCTAC TACGAGGCTG 
     1441 ACAGCGATGT GGCCTCAGCC TCCTCCCCCT TCTGGATGGA GAACCCCTAC GTGAAAGTGG 
     1501 ACACCATTGC ACCCGATGAG AGCTTCTCGC GGCTGGATGC CGGCCGTGTC AACACCAAGG 
     1561 TGCGCAGCTT TGGGCCACTT TCCAAGGCTG GCTTCTACCT GGCCTTCCAG GACCAGGGCG 
     1621 CCTGCATGTC GCTCATCTCC GTGCGCGCCT TCTACAAGAA GTGTGCATCC ACCACCGCAG 
     1681 GCTTCGCACT CTTCCCCGAG ACCCTCACTG GGGCGGAGCC CACCTCGCTG GTCATTGCTC 
     1741 CTGGCACCTG CATCCCTAAC GCCGTGGAGG TGTCGGTGCC ACTCAAGCTC TACTGCAACG 
     1801 GCGATGGGGA GTGGATGGTG CCTGTGGGTG CCTGCACCTG TGCCACCGGC CATGAGCCAG 
     1861 CTGCCAAGGA GTCCCAGTGC CGCCCCTGTC CCCCTGGGAG CTACAAGGCG AAGCAGGGAG 
     1921 AGGGGCCCTG CCTCCCATGT CCCCCCAACA GCCGTACCAC CTCCCCAGCC GCCAGCATCT 
     1981 GCACCTGCCA CAATAACTTC TACCGTGCAG ACTCGGACTC TGCGGACAGT GCCTGTACCA 
     2041 CCGTGCCATC TCCACCCCGA GGTGTGATCT CCAATGTGAA TGAAACCTCA CTGATCCTCG 
     2101 AGTGGAGTGA GCCCCGGGAC CTGGGTGGCC GGGATGACCT CCTGTACAAT GTCATCTGCA 
     2161 AGAAGTGCCA TGGGGCTGGA GGGGCCTCAG CCTGCTCACG CTGTGATGAC AACGTGGAGT 
     2221 TTGTGCCTCG GCAGCTGGGC CTGACGGAGC GCCGGGTCCA CATCAGCCAT CTGCTGGCCC 
     2281 ACACGCGCTA CACCTTTGAG GTGCAGGCGG TCAACGGTGT CTCGGGCAAG AGCCCTCTGC 
     2341 CGCCTCGTTA TGCGGCCGTG AATATCACCA CAAACCAGGC TGCCCCGTCT GAAGTGCCCA 
     2401 CACTACGCCT GCACAGCAGC TCAGGCAGCA GCCTCACCCT ATCCTGGGCA CCCCCAGAGC 
     2461 GGCCCAACGG AGTCATCCTG GACTACGAGA TGAAGTACTT TGAGAAGAGC GAGGGCATCG 
     2521 CCTCCACAGT GACCAGCCAG ATGAACTCCG TGCAGCTGGA CGGGCTTCGG CCTGACGCCC 
     2581 GCTATGTGGT CCAGGTCCGT GCCCGCACAG TAGCTGGCTA TGGGCAGTAC AGCCGCCCTG 
     2641 CCGAGTTTGA GACCACAAGT GAGAGAGGCT CTGGGGCCCA GCAGCTCCAG GAGCAGCTTC 
     2701 CCCTCATCGT GGGCTCCGCT ACAGCTGGGC TTGTCTTCGT GGTGGCTGTC GTGGTCATCG 
     2761 CTATCGTCTG CCTCAGGAAG CAGCGACACG GCTCTGATTC GGAGTACACG GAGAAGCTGC 
     2821 AGCAGTACAT TGCTCCTGGA ATGAAGGTTT ATATTGACCC TTTTACCTAC GAGGACCCTA 
     2881 ATGAGGCTGT TCGGGAGTTT GCCAAGGAGA TCGACGTGTC CTGCGTCAAG ATCGAGGAGG 
     2941 TGATCGGAGC TGGGGAATTT GGGGAAGTGT GCCGTGGTCG ACTGAAACAG CCTGGCCGCC 
     3001 GAGAGGTGTT TGTGGCCATC AAGACGCTGA AGGTGGGCTA CACCGAGAGG CAGCGGCGGG 
     3061 ACTTCCTAAG CGAGGCCTCC ATCATGGGTC AGTTTGATCA CCCCAATATA ATCCGGCTCG 
     3121 AGGGCGTGGT CACCAAAAGT CGGCCAGTTA TGATCCTCAC TGAGTTCATG GAAAACTGCG 
     3181 CCCTGGACTC CTTCCTCCGG CTCAACGATG GGCAGTTCAC GGTCATCCAG CTGGTGGGCA 
     3241 TGTTGCGGGG CATTGCTGCC GGCATGAAGT ACCTGTCCGA GATGAACTAT GTGCACCGCG 
     3301 ACCTGGCTGC TCGCAACATC CTTGTCAACA GCAACCTGGT CTGCAAAGTC TCAGACTTTG 
     3361 GCCTCTCCCG CTTCCTGGAG GATGACCCCT CCGATCCTAC CTACACCAGT TCCCTGGGCG 
     3421 GGAAGATCCC CATCCGCTGG ACTGCCCCAG AGGCCATAGC CTATCGGAAG TTCACTTCTG 
     3481 CTAGTGATGT CTGGAGCTAC GGAATTGTCA TGTGGGAGGT CATGAGCTAT GGAGAGCGAC 
     3541 CCTACTGGGA CATGAGCAAC CAGGATGTCA TCAATGCCGT GGAGCAGGAT TACCGGCTGC 
     3601 CACCACCCAT GGACTGTCCC ACAGCACTGC ACCAGCTCAT GCTGGACTGC TGGGTGCGGG 
     3661 ACCGGAACCT CAGGCCCAAA TTCTCCCAGA TTGTCAATAC CCTGGACAAG CTCATCCGCA 
     3721 ATGCTGCCAG CCTCAAGGTC ATTGCCAGCG CTCAGTCTGG CATGTCACAG CCCCTCCTGG 
     3781 ACCGCACGGT CCCAGATTAC ACAACCTTCA CGACAGTTGG TGATTGGCTG GATGCCATCA 
     3841 AGATGGGGCG GTACAAGGAG AGCTTCGTCA GTGCGGGGTT TGCATCTTTT GACCTGGTGG 
     3901 CCCAGATGAC GGCAGAAGAC CTGCTCCGTA TTGGGGTCAC CCTGGCCGGC CACCAGAAGA 
     3961 AGATCCTGAG CAGTATCCAG GACATGCGGC TGCAGATGAA CCAGACGCTG CCTGTGCAGG 
     4021 TCACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4081 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4141 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4201 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4261 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4321 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4381 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4441 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4501 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4561 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4621 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4681 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4741 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     4801 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     4861 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     4921 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     4981 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5041 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5101 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5161 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5221 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5281 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5341 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5401 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5461 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5521 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5581 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     5641 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     5701 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     5761 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     5821 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     5881 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     5941 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6001 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6061 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6121 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6181 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6241 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6301 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6361 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6421 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6481 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6541 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6601 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     6661 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     6721 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     6781 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     6841 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     6901 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     6961 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7021 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7081 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7141 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7201 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7261 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7321 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7381 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7441 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7501 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7561 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7621 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     7681 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     7741 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     7801 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     7861 TTTAACGCGA ATTTT
//