LOCUS       RC208361                7815 bp    DNA     circular  SYN 
DEFINITION  PEX6 (Myc-DDK-tagged)-Human peroxisomal biogenesis factor 6 (PEX6)
ACCESSION   NM_000287
VERSION     NM_000287.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3970..4061 
                     /label="Myc-DDK"  
     misc_feature    4091..4676 
                     /label="PolyA signal"  
     misc_feature    4148..4167 
                     /label="Restriction Enzyme"  
     misc_feature    4952..5624 
                     /label="ColE1"  
     misc_feature    6054..6848 
                     /label="Kan/Neo"  
     misc_feature    6883..7185 
                     /label="SV40 ori"  
     misc_feature    7271..7748 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3960..3968 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3960 
                     /label="PEX6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGCTGGCT GTCTTGCGGG TCCTGGAGCC CTTTCCGACC GAGACACCCC 
     1081 CGTTGGCAGT GCTGCTGCCA CCCGGGGGCC CGTGGCCGGC GGCGGAGCTG GGCCTGGTGC 
     1141 TGGCCCTGAG GCCTGCAGGG GAGAGCCCGG CAGGGCCGGC GCTGCTGGTG GCAGCCCTGG 
     1201 AGGGGCCGGA CGCGGGCACC GAAGAGCAGG GTCCCGGGCC GCCGCAGCTA CTGGTTAGCC 
     1261 GCGCGCTGCT GCGGCTCCTG GCACTGGGCT CCGGGGCCTG GGTGCGGGCG CGGGCGGTGC 
     1321 GGCGGCCCCC GGCGCTAGGT TGGGCACTGC TTGGCACCTC GCTGGGGCCT GGGCTCGGAC 
     1381 CGCGAGTCGG GCCGCTGCTG GTGAGGCGCG GAGAGACCCT CCCAGTGCCC GGACCGCGGG 
     1441 TGCTGGAGAC GCGGCCGGCG TTGCAAGGGC TGCTGGGCCC AGGGACTCGG CTGGCTGTGA 
     1501 CTGAGCTCCG CGGGCGGGCC AGACTGTGTC CAGAGTCTGG GGACAGCAGT CGGCCCCCAC 
     1561 CCCCGCCCGT GGTGTCCTCC TTTGCGGTTT CTGGCACAGT GCGGCGACTC CAGGGAGTTC 
     1621 TGGGAGGGAC TGGAGATTCA CTAGGGGTGA GCCGGAGCTG TCTCCGTGGC CTTGGCCTCT 
     1681 TCCAGGGCGA ATGGGTGTGG GTGGCCCAGG CCAGAGAGTC ATCGAACACT TCACAGCCGC 
     1741 ACTTGGCTAG GGTGCAGGTC CTAGAACCTC GCTGGGACCT CTCTGATAGA CTGGGACCCG 
     1801 GCTCTGGACC GCTGGGAGAG CCCCTCGCTG ACGGACTGGC GCTTGTCCCT GCCACTTTGG 
     1861 CTTTTAATCT TGGCTGTGAC CCCCTGGAAA TGGGAGAGCT CAGAATTCAG AGGTACTTGG 
     1921 AAGGCTCCAT CGCCCCTGAA GACAAAGGAA GCTGCTCATT GCTGCCTGGG CCTCCATTTG 
     1981 CCAGAGAGTT ACACATCGAA ATTGTGTCTT CTCCCCACTA CAGCACTAAT GGAAATTATG 
     2041 ACGGTGTTCT TTACCGGCAC TTTCAGATAC CCAGGGTAGT CCAGGAAGGG GATGTTCTAT 
     2101 GTGTGCCAAC AATTGGGCAA GTAGAGATCC TGGAAGGAAG TCCAGAGAAA CTGCCCAGGT 
     2161 GGCGGGAAAT GTTTTTTAAA GTGAAGAAAA CAGTTGGGGA AGCTCCAGAT GGACCAGCCA 
     2221 GTGCCTACTT GGCCGACACC ACCCATACCT CCTTGTACAT GGTGGGTTCT ACCCTGAGCC 
     2281 CTGTTCCATG GCTCCCTTCA GAGGAATCCA CTCTCTGGAG CAGTTTGTCT CCTCCAGGCC 
     2341 TGGAGGCCTT GGTGTCTGAA CTCTGTGCTG TCCTGAAGCC TCGCCTCCAG CCAGGGGGTG 
     2401 CCCTGCTGAC AGGAACTAGC AGTGTCCTTC TACGGGGCCC CCCAGGCTGT GGGAAGACCA 
     2461 CAGTAGTTGC TGCTGCCTGT AGTCACCTTG GGCTCCACTT ACTGAAGGTG CCCTGCTCCA 
     2521 GCCTCTGTGC AGAAAGTAGT GGGGCTGTGG AGACAAAACT GCAGGCCATC TTCTCCCGGG 
     2581 CCCGCCGTTG CCGGCCTGCA GTCCTGTTGC TCACAGCTGT GGACCTTCTG GGCCGGGACC 
     2641 GTGATGGGCT GGGTGAGGAT GCCCGTGTGA TGGCTGTGCT GCGTCACCTC CTCCTCAATG 
     2701 AGGACCCCCT CAACAGCTGC CCTCCCCTCA TGGTTGTGGC CACCACAAGC CGGGCCCAGG 
     2761 ACCTGCCTGC TGATGTGCAG ACAGCATTTC CTCATGAGCT CGAGGTGCCT GCTCTGTCAG 
     2821 AGGGGCAGCG GCTCAGCATC CTGCGGGCCC TCACTGCCCA CCTTCCCCTG GGCCAGGAGG 
     2881 TGAACTTGGC ACAGCTAGCA CGGCGGTGTG CAGGCTTTGT GGTAGGGGAT CTCTATGCCC 
     2941 TTCTGACCCA CAGCAGCCGG GCAGCCTGCA CCAGGATCAA GAACTCAGGT TTGGCAGGTG 
     3001 GCTTGACTGA GGAGGATGAG GGGGAGCTGT GTGCTGCCGG CTTTCCTCTC CTGGCTGAGG 
     3061 ACTTTGGGCA GGCACTGGAG CAACTGCAGA CAGCTCACTC CCAGGCCGTT GGAGCCCCCA 
     3121 AGATCCCCTC AGTGTCCTGG CATGATGTGG GTGGGCTGCA GGAGGTGAAG AAGGAGATCC 
     3181 TGGAGACCAT TCAGCTCCCC CTGGAGCACC CTGAGCTACT GAGCCTGGGC CTGAGACGCT 
     3241 CAGGCCTTCT GCTCCATGGG CCCCCTGGCA CCGGCAAGAC CCTTCTGGCC AAGGCAGTAG 
     3301 CCACTGAGTG CAGCCTTACC TTCCTCAGCG TGAAGGGGCC AGAGCTCATT AACATGTATG 
     3361 TGGGCCAAAG TGAGGAGAAT GTGCGGGAAG TATTTGCCAG GGCCAGGGCT GCAGCTCCAT 
     3421 GCATTATCTT CTTTGATGAA CTGGACTCTT TGGCCCCAAG CCGGGGGCGA AGTGGAGATT 
     3481 CTGGAGGAGT GATGGACAGG GTGGTGTCTC AGCTCCTTGC CGAGCTAGAT GGGCTGCACA 
     3541 GCACTCAGGA TGTGTTTGTG ATTGGAGCCA CCAACAGACC AGATCTCCTG GACCCTGCCC 
     3601 TTCTGCGGCC TGGCAGATTT GACAAGCTGG TGTTTGTGGG GGCAAATGAG GACCGGGCCT 
     3661 CCCAGCTACG CGTTCTAAGT GCCATCACAC GCAAATTCAA GCTAGAGCCA TCTGTGAGCC 
     3721 TGGTAAACGT GCTAGATTGC TGCCCTCCCC AGCTGACGGG CGCGGACCTC TACTCTCTCT 
     3781 GCTCTGATGC TATGACAGCT GCCCTCAAAC GCAGGGTTCA TGACCTGGAG GAAGGGCTGG 
     3841 AGCCAGGTAG CTCAGCACTG ATGCTCACCA TGGAGGACTT GCTGCAGGCT GCCGCCCGGC 
     3901 TGCAACCCTC AGTCAGTGAG CAGGAGCTGC TCCGGTACAA GCGCATCCAG CGCAAGTTTG 
     3961 CTGCCTGCAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4021 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4081 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4141 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4201 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4261 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4321 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4381 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4441 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4501 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4561 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4621 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4681 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     4741 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     4801 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     4861 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     4921 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     4981 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5041 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5101 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5161 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5221 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5281 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5341 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5401 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5461 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5521 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     5581 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     5641 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     5701 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     5761 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     5821 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     5881 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     5941 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6001 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6061 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6121 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6181 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6241 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6301 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6361 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6421 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6481 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6541 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     6601 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     6661 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     6721 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     6781 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     6841 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     6901 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     6961 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7021 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7081 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7141 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7201 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7261 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7321 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7381 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7441 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7501 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7561 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     7621 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     7681 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     7741 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     7801 TTTAACGCGA ATTTT
//