LOCUS       RC208308                8082 bp    DNA     circular  SYN 
DEFINITION  MICAL1 (Myc-DDK-tagged)-Human microtubule associated monoxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant 1
ACCESSION   NM_022765
VERSION     NM_022765.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4239..4330 
                     /label="Myc-DDK"  
     misc_feature    4360..4945 
                     /label="PolyA signal"  
     misc_feature    4417..4436 
                     /label="Restriction Enzyme"  
     misc_feature    5221..5893 
                     /label="ColE1"  
     misc_feature    6323..7117 
                     /label="Kan/Neo"  
     misc_feature    7152..7454 
                     /label="SV40 ori"  
     misc_feature    7540..8017 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4221..4227 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4221 
                     /label="MICAL1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTTCACCT ACCTCCACCA ACCCAGCGCA TGCCCACTTT GAGAGCTTCC 
     1081 TGCAGGCCCA GCTGTGCCAG GACGTGCTGA GCAGCTTCCA GGAGCTGTGT GGGGCCCTGG 
     1141 GGCTGGAACC CGGTGGGGGG CTGCCCCAGT ACCACAAGAT CAAGGACCAG CTCAACTACT 
     1201 GGAGCGCCAA GTCACTGTGG ACCAAGCTGG ACAAGCGAGC AGGCCAGCCT GTCTACCAGC 
     1261 AGGGCCGGGC CTGCACCAGC ACCAAGTGCC TGGTGGTGGG TGCTGGACCT TGCGGGCTGC 
     1321 GGGTCGCTGT GGAGCTGGCG CTGCTGGGGG CCCGAGTGGT GCTGGTGGAA AAGCGCACCA 
     1381 AGTTCTCTCG CCACAACGTG CTCCACCTCT GGCCCTTCAC CATCCACGAC CTGCGGGCAC 
     1441 TCGGTGCTAA GAAGTTCTAC GGGCGCTTCT GCACCGGCAC CCTGGACCAC ATCAGCATCA 
     1501 GGCAGCTCCA GCTGCTTCTG CTGAAGGTAG CATTGCTGCT GGGGGTGGAA ATTCACTGGG 
     1561 GTGTCACTTT CACTGGCCTC CAGCCCCCTC CTAGGAAGGG GAGTGGCTGG CGTGCCCAGC 
     1621 TCCAACCCAA CCCCCCTGCC CAGCTGGCCA ACTATGAATT TGACGTCCTT ATCTCGGCTG 
     1681 CAGGAGGTAA ATTCGTCCCT GAAGGCTTCA AAGTTCGAGA AATGCGAGGC AAACTGGCCA 
     1741 TTGGCATCAC AGCCAACTTT GTGAATGGAC GCACCGTGGA GGAGACACAG GTGCCGGAGA 
     1801 TCAGTGGTGT AGCCAGGATC TACAACCAGA GCTTCTTCCA GAGCCTTCTC AAAGCCACAG 
     1861 GCATTGATCT GGAGAACATT GTGTACTACA AGGACGACAC CCACTACTTT GTGATGACAG 
     1921 CCAAGAAGCA GTGCCTGCTG CGGCTGGGGG TGCTGCGCCA GGACTGGCCA GACACCAATC 
     1981 GGCTGCTGGG CAGTGCCAAT GTGGTGCCCG AGGCTCTGCA GCGCTTTACC CGGGCAGCTG 
     2041 CTGACTTTGC CACCCATGGC AAGCTCGGGA AACTAGAGTT TGCCCAGGAT GCCCATGGGC 
     2101 AGCCTGATGT CTCTGCCTTT GACTTCACGA GCATGATGCG GGCAGAGAGT TCTGCTCGTG 
     2161 TGCAAGAGAA GCATGGCGCC CGCCTGCTGC TGGGACTGGT GGGGGACTGC CTGGTGGAGC 
     2221 CCTTCTGGCC CCTGGGCACT GGAGTGGCAC GGGGCTTCCT GGCAGCCTTT GATGCAGCCT 
     2281 GGATGGTGAA GCGGTGGGCA GAGGGCGCTG AGTCCCTAGA GGTGTTGGCT GAGCGTGAGA 
     2341 GCCTGTACCA GCTTCTGTCA CAGACATCCC CAGAAAACAT GCATCGCAAT GTGGCCCAGT 
     2401 ATGGGCTGGA CCCAGCCACC CGCTACCCCA ACCTGAACCT CCGGGCAGTG ACCCCCAATC 
     2461 AGGTACGAGA CCTGTATGAT GTGCTAGCCA AGGAGCCTGT GCAGAGGAAC AACGACAAGA 
     2521 CAGATACAGG GATGCCAGCC ACCGGGTCGG CAGGCACCCA GGAGGAGCTG CTACGCTGGT 
     2581 GCCAGGAGCA GACAGCTGGG TACCCGGGAG TCCACGTCTC CGATTTGTCT TCCTCCTGGG 
     2641 CTGATGGGCT AGCTCTGTGT GCCCTGGTGT ACCGGCTGCA GCCTGGCCTG CTGGAACCCT 
     2701 CAGAGCTGCA GGGGCTGGGA GCTCTGGAAG CAACTGCTTG GGCACTAAAG GTGGCAGAGA 
     2761 ATGAGCTGGG CATCACACCG GTGGTGTCTG CACAGGCCGT GGTAGCAGGG AGTGACCCAC 
     2821 TGGGCCTCAT TGCCTACCTC AGCCACTTCC ACAGTGCCTT CAAGAGCATG GCCCACAGCC 
     2881 CAGGCCCTGT CAGCCAGGCC TCCCCAGGGA CCTCCAGTGC TGTATTATTC CTTAGTAAAC 
     2941 TTCAGAGGAC CCTGCAGCGA TCCCGGGCCA AGGAAAATGC AGAGGATGCT GGTGGCAAGA 
     3001 AGCTGCGCTT GGAGATGGAG GCCGAGACCC CAAGTACTGA GGTGCCACCT GACCCAGAGC 
     3061 CTGGTGTACC CCTGACACCC CCATCCCAAC ACCAGGAGGC CGGTGCTGGG GACCTGTGTG 
     3121 CACTTTGTGG GGAACACCTC TATGTCCTGG AACGCCTCTG TGTCAACGGC CATTTCTTCC 
     3181 ACCGGAGCTG CTTCCGCTGC CATACCTGTG AGGCCACACT GTGGCCAGGT GGCTACGAGC 
     3241 AGCACCCAGG AGATGGACAT TTCTACTGCC TCCAGCACCT GCCCCAGACA GACCACAAAG 
     3301 CGGAAGGCAG CGATAGAGGC CCTGAGAGTC CGGAGCTCCC CACACCAAGT GAGAATAGCA 
     3361 TGCCACCAGG CCTCTCAACT CCCACAGCCT CGCAGGAGGG GGCCGGTCCT GTTCCAGATC 
     3421 CCAGCCAGCC CACCCGTCGG CAGATCCGCC TCTCCAGCCC GGAGCGCCAG CGGTTGTCCT 
     3481 CCCTTAACCT TACCCCTGAC CCGGAAATGG AGCCTCCACC CAAGCCTCCC CGCAGCTGCT 
     3541 CCGCCTTGGC CCGCCACGCC CTGGAGAGCA GCTTTGTGGG CTGGGGCCTG CCAGTCCAGA 
     3601 GCCCTCAAGC TCTTGTGGCC ATGGAGAAGG AGGAAAAAGA GAGTCCCTTC TCCAGTGAAG 
     3661 AGGAAGAAGA AGATGTGCCT TTGGACTCAG ATGTGGAACA GGCCCTGCAG ACCTTTGCCA 
     3721 AGACCTCAGG CACCATGAAT AACTACCCAA CATGGCGTCG GACTCTGCTG CGCCGTGCGA 
     3781 AGGAGGAGGA GATGAAGAGG TTCTGCAAGG CCCAGACCAT CCAACGGCGA CTAAATGAGA 
     3841 TTGAGGCTGC CTTGAGGGAG CTAGAGGCCG AGGGCGTGAA GCTGGAGCTG GCCTTGAGGC 
     3901 GCCAGAGCAG TTCCCCAGAA CAGCAAAAGA AACTATGGGT AGGACAGCTG CTACAGCTCG 
     3961 TTGACAAGAA AAACAGCCTG GTGGCTGAGG AGGCCGAGCT CATGATCACG GTGCAGGAAT 
     4021 TGAATCTGGA GGAGAAACAG TGGCAGCTGG ACCAGGAGCT ACGAGGCTAC ATGAACCGGG 
     4081 AAGAAAACCT AAAGACAGCT GCTGATCGGC AGGCTGAGGA CCAGGTCCTG AGGAAGCTGG 
     4141 TGGATTTGGT CAACCAGAGA GATGCCCTCA TCCGCTTCCA GGAGGAGCGC AGGCTCAGCG 
     4201 AGCTGGCCTT GGGGACAGGG GCCCAGGGCA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4261 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4321 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4381 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4441 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4501 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4561 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4621 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4681 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4741 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4801 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4861 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4921 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4981 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5041 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5101 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5161 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5221 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5281 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5341 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5401 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5461 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5521 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5581 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5641 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5701 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     5761 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     5821 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     5881 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     5941 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6001 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6061 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6121 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6181 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6241 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6301 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6361 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6421 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6481 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6541 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6601 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6661 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6721 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6781 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6841 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6901 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6961 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7021 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7081 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7141 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7201 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7261 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7321 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7381 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7441 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7501 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7561 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7621 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7681 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7741 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7801 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7861 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7921 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7981 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8041 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//