LOCUS       RC208263                7149 bp    DNA     circular  SYN 
DEFINITION  PLCD1 (Myc-DDK-tagged)-Human phospholipase C, delta 1 (PLCD1), transcript variant 2
ACCESSION   NM_006225
VERSION     NM_006225.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3306..3397 
                     /label="Myc-DDK"  
     misc_feature    3427..4012 
                     /label="PolyA signal"  
     misc_feature    3484..3503 
                     /label="Restriction Enzyme"  
     misc_feature    4288..4960 
                     /label="ColE1"  
     misc_feature    5390..6184 
                     /label="Kan/Neo"  
     misc_feature    6219..6521 
                     /label="SV40 ori"  
     misc_feature    6607..7084 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3288..3294 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3288 
                     /label="PLCD1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACTCGGGC CGGGACTTCC TGACCCTGCA CGGCCTACAG GATGATGAGG 
     1081 ATCTACAGGC GCTGCTGAAG GGCAGCCAGC TCCTGAAGGT GAAGTCCAGC TCATGGAGGA 
     1141 GAGAGCGCTT CTACAAGTTG CAGGAGGACT GCAAGACCAT CTGGCAGGAG TCCCGCAAGG 
     1201 TCATGCGGAC CCCGGAGTCC CAGCTGTTCT CCATCGAGGA CATTCAGGAG GTGCGAATGG 
     1261 GGCACCGCAC GGAGGGTCTG GAGAAGTTCG CCCGTGATGT GCCCGAGGAC CGCTGCTTCT 
     1321 CCATTGTCTT CAAGGACCAG CGCAATACAC TAGACCTCAT CGCCCCATCG CCAGCTGATG 
     1381 CCCAGCACTG GGTGCTGGGG CTGCACAAGA TCATCCACCA CTCAGGCTCC ATGGACCAGC 
     1441 GTCAGAAGCT ACAGCACTGG ATTCACTCCT GCTTGCGAAA AGCTGACAAA AACAAGGACA 
     1501 ACAAGATGAG CTTCAAGGAG CTGCAGAACT TCCTGAAGGA GCTCAACATC CAGGTGGACG 
     1561 ACAGCTATGC CCGGAAGATC TTCAGGGAGT GTGACCACTC CCAGACAGAC TCCCTGGAGG 
     1621 ACGAGGAGAT TGAGGCCTTC TACAAGATGC TGACCCAGCG GGTGGAGATC GACCGCACCT 
     1681 TCGCCGAGGC CGCGGGCTCA GGGGAGACTC TGTCGGTGGA TCAGTTAGTG ACGTTCCTGC 
     1741 AGCACCAGCA GCGGGAGGAG GCGGCAGGGC CTGCGCTGGC CCTCTCCCTC ATTGAGCGCT 
     1801 ACGAGCCCAG CGAGACTGCC AAGGCGCAGC GGCAGATGAC CAAGGACGGC TTCCTCATGT 
     1861 ACTTACTGTC GGCTGACGGC AGCGCCTTCA GCCTGGCACA CCGCCGTGTC TACCAGGACA 
     1921 TGGGCCAGCC ACTTAGCCAC TACCTGGTGT CCTCTTCACA CAACACCTAC CTGCTGGAGG 
     1981 ACCAGCTAGC CGGGCCCAGC AGCACTGAAG CCTACATCCG GGCACTGTGC AAAGGCTGCC 
     2041 GATGCCTGGA GCTTGACTGC TGGGACGGGC CCAACCAGGA ACCAATCATC TACCACGGCT 
     2101 ATACTTTCAC TTCCAAGATC CTCTTCTGCG ATGTGCTCAG GGCCATCCGG GACTATGCCT 
     2161 TCAAGGCGTC CCCCTACCCT GTCATCCTAT CCCTGGAGAA CCACTGCACA CTGGAGCAGC 
     2221 AGCGCGTGAT GGCGCGGCAC CTGCATGCCA TCCTGGGCCC CATGCTGTTG AACCGACCAC 
     2281 TGGATGGGGT CACCAACAGC CTGCCCTCCC CTGAGCAACT GAAGGGGAAG ATCCTGCTGA 
     2341 AGGGGAAGAA GCTCGGGGGG CTCCTGCCCC CTGGAGGGGA GGGTGGCCCT GAGGCCACTG 
     2401 TGGTGTCAGA CGAAGACGAG GCTGCTGAGA TGGAGGATGA GGCAGTGAGG AGCCGTGTGC 
     2461 AGCACAAGCC CAAGGAGGAC AAGCTCAGGC TAGCACAGGA GCTCTCTGAC ATGGTCATTT 
     2521 ACTGCAAGAG TGTCCACTTT GGGGGCTTCT CCAGTCCTGG CACCCCTGGA CAGGCCTTCT 
     2581 ACGAGATGGC GTCCTTCTCT GAGAACCGTG CCCTTCGACT GCTCCAAGAA TCAGGAAACG 
     2641 GCTTTGTCCG CCACAACGTG GGGCACCTGA GCAGAATCTA CCCGGCTGGA TGGAGAACAG 
     2701 ACTCCTCCAA CTACAGCCCC GTGGAGATGT GGAATGGGGG CTGCCAGATC GTGGCCCTGA 
     2761 ATTTCCAGAC ACCTGGGCCA GAGATGGACG TGTACCAGGG CCGCTTCCAG GACAACGGGG 
     2821 CCTGTGGGTA CGTGCTGAAG CCCGCCTTCC TGCGAGACCC CAACGGCACC TTTAACCCCC 
     2881 GCGCCCTGGC TCAGGGGCCC TGGTGGGCAC GGAAGCGGCT CAACATCAGG GTCATTTCGG 
     2941 GGCAGCAGCT GCCAAAAGTC AACAAGAATA AGAATTCAAT TGTGGACCCC AAAGTGACAG 
     3001 TGGAGATCCA TGGCGTGAGC CGGGACGTGG CCAGCCGCCA GACTGCTGTC ATCACCAACA 
     3061 ATGGTTTCAA CCCATGGTGG GACACGGAGT TTGCGTTTGA GGTAGTTGTG CCTGACCTTG 
     3121 CCCTCATCCG CTTCTTGGTG GAAGATTATG ATGCCTCCTC CAAGAATGAC TTCATTGGCC 
     3181 AGAGTACCAT CCCCTTGAAC AGCCTCAAGC AAGGATACCG CCATGTCCAC CTCATGTCTA 
     3241 AGAACGGGGA CCAGCATCCA TCAGCCACCC TCTTTGTGAA GATCTCCCTC CAGGACACGC 
     3301 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     3361 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     3421 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     3481 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     3541 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     3601 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     3661 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     3721 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     3781 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     3841 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     3901 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     3961 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4021 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     4081 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     4141 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     4201 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     4261 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     4321 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     4381 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     4441 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     4501 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     4561 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     4621 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     4681 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     4741 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     4801 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     4861 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     4921 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     4981 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     5041 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     5101 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     5161 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     5221 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     5281 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     5341 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     5401 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     5461 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     5521 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     5581 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     5641 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     5701 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     5761 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     5821 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     5881 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     5941 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     6001 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     6061 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     6121 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     6181 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     6241 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     6301 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     6361 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     6421 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     6481 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6541 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     6601 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     6661 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     6721 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     6781 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     6841 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     6901 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     6961 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     7021 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     7081 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     7141 GCGAATTTT
//