LOCUS       RC208227                8988 bp    DNA     circular  SYN 
DEFINITION  DHX29 (Myc-DDK-tagged)-Human DEAH (Asp-Glu-Ala-His) box polypeptide 29 (DHX29)
ACCESSION   NM_019030
VERSION     NM_019030.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5145..5236 
                     /label="Myc-DDK"  
     misc_feature    5266..5851 
                     /label="PolyA signal"  
     misc_feature    5323..5342 
                     /label="Restriction Enzyme"  
     misc_feature    6127..6799 
                     /label="ColE1"  
     misc_feature    7229..8023 
                     /label="Kan/Neo"  
     misc_feature    8058..8360 
                     /label="SV40 ori"  
     misc_feature    8446..8923 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5127..5133 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5127 
                     /label="DHX29"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCGGCAAG AACAAGAAAC ACAAGGCTCC AGCGGCCGCG GTGGTCCGGG 
     1081 CCGCCGTGTC TGCTTCCAGA GCCAAATCTG CCGAGGCTGG AATTGCCGGG GAGGCCCAAA 
     1141 GCAAGAAGCC AGTGTCCAGG CCGGCCACCG CTGCCGCTGC CGCTGCCGGC TCCAGGGAGC 
     1201 CCCGTGTCAA GCAAGGTCCA AAAATTTATA GTTTTAATTC TACAAATGAT TCTAGTGGTC 
     1261 CTGCAAATCT GGATAAATCT ATTTTGAAAG TGGTAATTAA TAACAAACTA GAGCAAAGAA 
     1321 TTATTGGAGT GATCAATGAG CATAAAAAGC AAAATAATGA CAAAGGAATG ATTTCTGGAA 
     1381 GACTTACTGC CAAAAAATTG CAGGATTTAT ACATGGCTTT ACAAGCATTT TCATTTAAGA 
     1441 CAAAGGACAT TGAAGATGCC ATGACCAATA CACTCTTATA TGGAGGTGAC CTTCATTCTG 
     1501 CCTTGGATTG GCTCTGTTTA AACCTTTCAG ATGATGCACT TCCTGAAGGA TTCAGTCAGG 
     1561 AATTTGAAGA GCAGCAACCT AAAAGTAGGC CTAAATTTCA GTCTCCTCAA ATACAAGCCA 
     1621 CTATTTCACC TCCATTGCAA CCTAAAACAA AAACATATGA AGAGGACCCT AAGAGTAAGC 
     1681 CAAAAAAGGA AGAAAAAAAT ATGGAAGTAA ATATGAAAGA GTGGATTTTA CGATATGCTG 
     1741 AACAACAAAA TGAAGAAGAG AAGAATGAGA ATTCTAAAAG TTTAGAAGAG GAGGAAAAAT 
     1801 TTGACCCTAA TGAAAGGTAC TTACATCTTG CAGCAAAACT GCTGGATGCA AAAGAACAAG 
     1861 CAGCTACCTT TAAACTAGAA AAAAACAAGC AAGGCCAAAA AGAGGCTCAG GAAAAAATAA 
     1921 GGAAATTTCA AAGAGAAATG GAAACTTTAG AAGACCATCC AGTATTTAAC CCAGCCATGA 
     1981 AGATTTCACA TCAACAAAAT GAAAGGAAAA AGCCTCCTGT AGCCACAGAA GGAGAAAGTG 
     2041 CATTGAATTT TAATTTATTT GAAAAATCTG CAGCTGCTAC TGAAGAAGAG AAAGATAAAA 
     2101 AGAAAGAACC TCATGATGTA AGAAATTTTG ACTATACTGC TCGAAGTTGG ACTGGAAAAT 
     2161 CTCCCAAACA ATTTCTGATT GATTGGGTCA GGAAGAATCT TCCCAAGAGT CCAAATCCTT 
     2221 CCTTTGAAAA AGTTCCAGTA GGTAGATACT GGAAATGTAG GGTTAGGGTA ATCAAGTCTG 
     2281 AAGATGATGT CCTGGTAGTA TGCCCTACAA TCTTAACAGA AGATGGCATG CAAGCTCAGC 
     2341 ACCTGGGAGC TACTTTAGCC CTTTACCGTT TAGTTAAAGG GCAGTCAGTT CATCAGTTAC 
     2401 TTCCTCCCAC TTACCGAGAT GTTTGGCTGG AGTGGAGTGA TGCAGAAAAG AAAAGGGAAG 
     2461 AATTAAATAA AATGGAAACC AATAAACCAC GTGATCTTTT TATTGCCAAA CTTCTGAATA 
     2521 AACTGAAACA GCAGCAACAG CAGCAACAAC AGCATTCTGA AAATAAGAGA GAAAACTCTG 
     2581 AAGATCCCGA GGAATCTTGG GAAAATTTAG TTTCGGATGA GGATTTTTCT GCACTGTCCT 
     2641 TGGAATCAGC AAATGTGGAA GATTTGGAAC CTGTTAGAAA CCTCTTTAGA AAGTTGCAAA 
     2701 GCACACCTAA GTATCAGAAA CTTCTAAAGG AAAGACAACA GCTACCTGTA TTTAAACATC 
     2761 GGGACTCAAT TGTTGAAACT CTTAAAAGGC ATCGGGTAGT GGTTGTGGCA GGTGAAACAG 
     2821 GGAGTGGTAA AAGTACTCAG GTACCACATT TTCTATTGGA AGATTTGCTT CTAAATGAGT 
     2881 GGGAAGCAAG TAAATGTAAC ATTGTCTGTA CCCAACACCG AAGAATCTCA GCAGTTAGTT 
     2941 TAGCCAACAG AGTATGTGAT GAATTGGGCT GTGAAAATGG ACCTGGAGGA AGGAATTCCT 
     3001 TGTGTGGATA TCAGATCCGG ATGGAATCTC GAGCTTGTGA ATCTACCAGG TTACTCTATT 
     3061 GTACAACAGG GGTTTTGCTA AGGAAACTTC AAGAAGATGG TCTTCTAAGT AATGTGTCTC 
     3121 ATGTTATTGT AGATGAGGTT CATGAAAGAA GTGTCCAGTC AGACTTCCTA CTAATTATCT 
     3181 TGAAGGAAAT TTTACAGAAA CGTTCTGATC TACACTTGAT TCTAATGAGT GCCACTGTGG 
     3241 ACAGCGAAAA ATTTTCTACA TATTTCACAC ACTGCCCCAT TCTCAGAATT TCAGGAAGGA 
     3301 GTTATCCTGT TGAGGTTTTT CATCTTGAAG ATATAATAGA AGAAACAGGC TTTGTACTGG 
     3361 AAAAAGACTC AGAATATTGT CAGAAATTTC TGGAAGAGGA AGAAGAAGTA ACCATTAATG 
     3421 TTACAAGCAA AGCAGGGGGA ATAAAAAAAT ATCAGGAATA CATCCCAGTT CAGACTGGAG 
     3481 CACATGCTGA TTTAAATCCA TTTTACCAAA AGTACAGCAG CCGCACTCAG CATGCTATTC 
     3541 TATACATGAA TCCTCATAAA ATCAACCTGG ATCTCATTTT GGAACTTCTT GCATACTTAG 
     3601 ATAAAAGTCC CCAATTCAGA AATATTGAAG GAGCAGTATT GATCTTTTTA CCAGGACTTG 
     3661 CTCATATTCA GCAGTTGTAT GATCTTCTAT CAAATGATAG AAGATTTTAT TCTGAACGAT 
     3721 ATAAAGTGAT AGCTCTGCAT TCTATTCTTT CAACCCAAGA TCAAGCTGCA GCATTCACAC 
     3781 TTCCCCCTCC AGGAGTCAGG AAGATTGTTT TAGCAACCAA TATTGCAGAG ACGGGTATCA 
     3841 CTATTCCTGA TGTTGTATTT GTAATTGATA CTGGAAGAAC AAAAGAAAAT AAGTACCATG 
     3901 AAAGCAGTCA GATGAGTTCT TTGGTGGAGA CGTTTGTCAG TAAAGCCAGT GCTTTGCAGC 
     3961 GCCAGGGAAG AGCTGGGCGG GTCAGAGATG GCTTCTGTTT CCGAATGTAC ACAAGAGAAA 
     4021 GATTTGAAGG CTTTATGGAT TATTCTGTTC CTGAAATCTT ACGTGTACCT TTGGAGGAAT 
     4081 TATGCCTTCA TATTATGAAA TGTAATCTTG GTTCTCCTGA AGATTTCCTC TCCAAAGCCT 
     4141 TAGATCCTCC TCAGCTCCAA GTGATCAGCA ATGCAATGAA TTTGCTCCGA AAAATTGGAG 
     4201 CTTGTGAATT AAATGAGCCT AAACTGACTC CGTTGGGCCA ACACCTTGCA GCTTTACCTG 
     4261 TGAATGTCAA GATTGGCAAG ATGCTTATTT TTGGTGCCAT ATTTGGCTGC CTTGACCCAG 
     4321 TGGCAACACT AGCTGCAGTT ATGACAGAGA AGTCTCCTTT TACCACACCA ATTGGTCGAA 
     4381 AAGATGAAGC AGATCTTGCA AAATCAGCTT TGGCCATGGC GGATTCAGAC CACCTGACGA 
     4441 TCTACAATGC ATATCTAGGA TGGAAGAAAG CACGACAAGA AGGAGGTTAT CGTTCTGAAA 
     4501 TCACATACTG CCGGAGGAAC TTTCTTAATA GAACATCACT GTTAACCCTA GAGGATGTAA 
     4561 AGCAGGAGTT AATAAAGTTG GTTAAGGCAG CAGGATTTTC ATCTTCCACA ACTTCTACCA 
     4621 GCTGGGAAGG AAACAGAGCC TCACAGACCC TCTCATTCCA AGAAATTGCC CTTCTTAAAG 
     4681 CTGTACTGGT GGCTGGACTG TATGACAATG TGGGGAAGAT AATCTATACA AAGTCAGTGG 
     4741 ATGTTACAGA AAAATTGGCT TGCATTGTGG AGACGGCCCA AGGCAAAGCA CAAGTACACC 
     4801 CATCCTCAGT AAATCGAGAT TTGCAAACTC ATGGATGGCT CTTATACCAG GAGAAGATAA 
     4861 GGTATGCCAG AGTGTATTTG AGAGAAACTA CCCTAATAAC CCCTTTTCCA GTTTTACTTT 
     4921 TTGGTGGTGA TATAGAAGTT CAGCACCGAG AACGTCTTCT TTCTATTGAT GGCTGGATCT 
     4981 ATTTTCAGGC CCCTGTAAAG ATAGCTGTCA TTTTCAAGCA GCTGAGAGTT CTCATTGATT 
     5041 CAGTTTTAAG AAAAAAGCTT GAAAATCCAA AGATGTCCCT TGAAAATGAC AAGATTCTGC 
     5101 AGATCATTAC GGAATTGATA AAAACAGAGA ATAACACGCG TACGCGGCCG CTCGAGCAGA 
     5161 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     5221 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     5281 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     5341 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     5401 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     5461 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     5521 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     5581 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5641 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5701 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5761 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5821 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5881 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5941 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     6001 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     6061 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     6121 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     6181 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     6241 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     6301 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     6361 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     6421 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     6481 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     6541 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     6601 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6661 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6721 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6781 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6841 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6901 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6961 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     7021 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     7081 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     7141 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     7201 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     7261 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     7321 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     7381 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     7441 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     7501 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     7561 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     7621 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7681 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7741 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7801 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7861 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7921 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7981 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     8041 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     8101 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     8161 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     8221 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8281 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     8341 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     8401 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     8461 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     8521 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     8581 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     8641 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8701 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8761 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8821 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8881 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8941 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//