LOCUS       RC208212                8571 bp    DNA     circular  SYN 
DEFINITION  CAND1 (Myc-DDK-tagged)-Human cullin-associated and neddylation-dissociated 1 (CAND1)
ACCESSION   NM_018448
VERSION     NM_018448.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4728..4819 
                     /label="Myc-DDK"  
     misc_feature    4849..5434 
                     /label="PolyA signal"  
     misc_feature    4906..4925 
                     /label="Restriction Enzyme"  
     misc_feature    5710..6382 
                     /label="ColE1"  
     misc_feature    6812..7606 
                     /label="Kan/Neo"  
     misc_feature    7641..7943 
                     /label="SV40 ori"  
     misc_feature    8029..8506 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4710..4716 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4710 
                     /label="CAND1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGAGCGCC TCGTACCACA TTTCCAATTT GCTGGAAAAA ATGACATCCA 
     1081 GCGACAAGGA CTTTAGGTTT ATGGCTACAA ATGATTTGAT GACGGAACTG CAGAAAGATT 
     1141 CCATCAAGTT GGATGATGAT AGTGAAAGGA AAGTAGTGAA AATGATTTTG AAGTTATTGG 
     1201 AAGATAAAAA TGGAGAGGTA CAGAATTTAG CTGTCAAATG TCTTGGTCCT TTAGTGAGTA 
     1261 AAGTGAAAGA ATACCAAGTA GAGACAATTG TAGATACCCT CTGCACTAAC ATGCTTTCTG 
     1321 ATAAAGAACA ACTTCGAGAC ATTTCAAGTA TTGGTCTTAA AACAGTAATT GGAGAACTTC 
     1381 CTCCAGCTTC CAGTGGCTCT GCATTAGCTG CTAATGTATG TAAAAAGATT ACTGGACGTC 
     1441 TTACAAGTGC AATAGCAAAA CAGGAAGATG TCTCTGTTCA GCTAGAAGCC TTGGATATTA 
     1501 TGGCTGATAT GTTGAGCAGG CAAGGAGGAC TTCTTGTTAA TTTCCATCCT TCAATTCTGA 
     1561 CCTGTCTACT TCCCCAGTTG ACCAGCCCTA GACTTGCAGT GAGGAAAAGA ACCATTATCG 
     1621 CTCTTGGCCA TCTGGTTATG AGCTGTGGAA ATATAGTTTT TGTAGATCTT ATTGAACATC 
     1681 TGTTGTCAGA GTTGTCCAAA AATGATTCTA TGTCAACAAC AAGAACCTAC ATACAATGTA 
     1741 TTGCTGCTAT TAGTAGGCAA GCTGGTCATA GAATAGGTGA ATACCTTGAG AAGATAATTC 
     1801 CTTTGGTGGT AAAATTTTGC AATGTAGATG ATGATGAATT AAGAGAGTAC TGTATTCAAG 
     1861 CCTTTGAATC ATTTGTAAGA AGATGTCCTA AGGAAGTATA TCCTCATGTT TCTACCATTA 
     1921 TAAATATTTG TCTTAAATAT CTTACCTATG ATCCAAATTA TAATTACGAT GATGAAGATG 
     1981 AAGATGAAAA TGCAATGGAT GCTGATGGTG GTGATGATGA TGATCAAGGG AGTGATGATG 
     2041 AATACAGTGA TGATGATGAC ATGAGTTGGA AAGTGAGACG TGCAGCTGCG AAGTGCTTGG 
     2101 ATGCTGTAGT TAGCACAAGG CATGAAATGC TTCCAGAATT CTACAAGACC GTCTCTCCTG 
     2161 CACTAATATC CAGATTTAAA GAGCGTGAAG AGAATGTAAA GGCAGATGTT TTTCACGCAT 
     2221 ACCTTTCTCT TTTGAAGCAA ACTCGTCCTG TACAAAGTTG GCTATGTGAC CCTGATGCAA 
     2281 TGGAGCAGGG AGAAACACCT TTAACAATGC TTCAGAGTCA GGTTCCCAAC ATTGTTAAAG 
     2341 CTCTTCACAA ACAGATGAAA GAAAAAAGTG TGAAGACCCG ACAGTGTTGT TTTAACATGT 
     2401 TAACTGAGCT GGTAAATGTA TTACCTGGGG CCCTAACTCA ACACATTCCT GTACTTGTAC 
     2461 CAGGAATCAT TTTCTCACTG AATGATAAAT CAAGCTCATC GAATTTGAAG ATCGATGCTT 
     2521 TGTCATGTCT ATACGTAATC CTCTGTAACC ATTCTCCTCA AGTCTTCCAT CCTCACGTTC 
     2581 AGGCTTTGGT TCCTCCAGTG GTGGCTTGTG TTGGAGACCC ATTTTACAAA ATTACATCTG 
     2641 AAGCACTTCT TGTTACTCAA CAGCTTGTCA AAGTAATTCG TCCTTTAGAT CAGCCTTCCT 
     2701 CGTTTGATGC AACTCCTTAT ATCAAAGATC TATTTACCTG TACCATTAAG AGATTAAAAG 
     2761 CAGCTGACAT TGATCAGGAA GTCAAGGAAA GGGCTATTTC CTGTATGGGA CAAATTATTT 
     2821 GCAACCTTGG AGACAATTTG GGTTCTGACT TGCCTAATAC ACTTCAGATT TTCTTGGAGA 
     2881 GACTAAAGAA TGAAATTACC AGGTTAACTA CAGTAAAGGC ATTGACACTG ATTGCTGGGT 
     2941 CACCTTTGAA GATAGATTTG AGGCCTGTTC TGGGAGAAGG GGTTCCTATC CTTGCTTCAT 
     3001 TTCTTAGAAA AAACCAGAGA GCTTTGAAAC TGGGTACTCT TTCTGCCCTT GATATTCTAA 
     3061 TAAAAAACTA TAGTGACAGC TTGACAGCTG CCATGATTGA TGCAGTTCTA GATGAGCTCC 
     3121 CACCTCTTAT CAGCGAAAGT GATATGCATG TTTCACAAAT GGCCATCAGT TTTCTTACCA 
     3181 CTTTGGCAAA AGTATATCCC TCCTCCCTTT CAAAGATAAG TGGATCCATT CTCAATGAAC 
     3241 TTATTGGACT TGTGAGATCA CCCTTATTGC AGGGGGGAGC TCTTAGTGCC ATGCTAGACT 
     3301 TTTTCCAAGC TCTGGTTGTC ACTGGAACAA ATAATTTAGG ATACATGGAT TTGTTGCGCA 
     3361 TGCTGACTGG TCCAGTTTAC TCTCAGAGCA CAGCTCTTAC TCATAAGCAG TCTTATTATT 
     3421 CCATTGCCAA ATGTGTAGCT GCCCTTACTC GAGCATGCCC TAAAGAGGGA CCAGCTGTAG 
     3481 TAGGTCAGTT TATTCAAGAT GTCAAGAACT CAAGGTCTAC AGATTCCATT CGTCTCTTAG 
     3541 CTCTACTTTC TCTTGGAGAA GTTGGGCATC ATATTGACTT AAGTGGACAG TTGGAACTAA 
     3601 AATCTGTAAT ACTAGAAGCT TTCTCATCTC CTAGTGAAGA AGTCAAATCA GCTGCATCCT 
     3661 ATGCATTAGG CAGCATTAGT GTGGGCAACC TTCCTGAATA TCTACCGTTT GTCCTGCAAG 
     3721 AAATAACTAG TCAACCCAAA AGGCAGTATC TTTTACTTCA TTCCTTGAAG GAAATTATTA 
     3781 GCTCTGCATC AGTGGTGGGC CTTAAACCAT ATGTTGAAAA CATCTGGGCC TTATTACTAA 
     3841 AGCACTGTGA GTGTGCAGAG GAAGGAACCA GAAATGTTGT TGTTGAATGT CTAGGAAAAC 
     3901 TCACTCTAAT TGATCCAGAA ACTCTCCTTC CACGGCTTAA GGGGTACTTG ATATCAGGCT 
     3961 CATCATATGC CCGAAGCTCA GTGGTTACGG CTGTGAAATT TACAATTTCT GACCATCCAC 
     4021 AACCTATTGA TCCACTGTTA AAGAACTGCA TAGGTGATTT CCTAAAAACT TTGGAAGACC 
     4081 CAGATTTGAA TGTGAGAAGA GTAGCCTTGG TCACATTTAA TTCAGCAGCA CATAACAAGC 
     4141 CATCATTAAT AAGGGATCTA TTGGATACTG TTCTTCCACA TCTTTACAAT GAAACAAAAG 
     4201 TTAGAAAGGA GCTTATAAGA GAGGTAGAAA TGGGTCCATT TAAACATACG GTTGATGATG 
     4261 GTCTGGATAT TAGAAAGGCA GCATTTGAGT GTATGTACAC ACTTCTAGAC AGTTGTCTTG 
     4321 ATAGACTTGA TATCTTTGAA TTTCTAAATC ATGTTGAAGA TGGTTTGAAG GACCATTATG 
     4381 ATATTAAGAT GCTGACATTT TTAATGTTGG TGAGACTGTC TACCCTTTGT CCAAGTGCAG 
     4441 TACTGCAGAG GTTGGACCGA CTTGTTGAGC CATTACGTGC AACATGTACA ACTAAGGTAA 
     4501 AGGCAAACTC AGTAAAGCAG GAGTTTGAAA AACAAGATGA ATTAAAGCGA TCTGCCATGA 
     4561 GAGCAGTAGC AGCACTACTA ACCATTCCAG AAGCAGAGAA GAGTCCACTG ATGAGTGAAT 
     4621 TCCAGTCACA GATCAGTTCT AACCCTGAGC TGGCGGCTAT CTTTGAAAGT ATCCAGAAAG 
     4681 ATTCATCATC TACTAACTTG GAATCAATGG ACACTAGTAC GCGTACGCGG CCGCTCGAGC 
     4741 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4801 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4861 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4921 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4981 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     5041 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     5101 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5161 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5221 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5281 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5341 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5401 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5461 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5521 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5581 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5641 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5701 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5761 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5821 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5881 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5941 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     6001 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6061 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6121 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6181 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6241 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6301 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6361 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6421 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6481 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6541 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6601 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6661 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6721 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6781 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6841 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6901 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6961 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     7021 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7081 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7141 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7201 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7261 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7321 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7381 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7441 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7501 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7561 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7621 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7681 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7741 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7801 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7861 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7921 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7981 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     8041 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     8101 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8161 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8221 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8281 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8341 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8401 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8461 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8521 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//