LOCUS       RC208206                8433 bp    DNA     circular  SYN 
DEFINITION  FBLN2 (Myc-DDK-tagged)-Human fibulin 2 (FBLN2), transcript variant 2
ACCESSION   NM_001998
VERSION     NM_001998.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4590..4681 
                     /label="Myc-DDK"  
     misc_feature    4711..5296 
                     /label="PolyA signal"  
     misc_feature    4768..4787 
                     /label="Restriction Enzyme"  
     misc_feature    5572..6244 
                     /label="ColE1"  
     misc_feature    6674..7468 
                     /label="Kan/Neo"  
     misc_feature    7503..7805 
                     /label="SV40 ori"  
     misc_feature    7891..8368 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4572..4578 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4572 
                     /label="FBLN2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGCTGCTC TGGGAGCCTG CAGGAGCCTG GCTTGCTCTG GGCCTGGCCC 
     1081 TGGCCCTGGG CCCCAGCGTG GCCGCAGCTG CCCCTCGGCA GGACTGCACG GGCGTGGAGT 
     1141 GCCCGCCGCT GGAGAACTGC ATTGAGGAGG CGCTGGAGCC GGGTGCCTGC TGTGCCACGT 
     1201 GTGTGCAGCA GGGCTGCGCC TGCGAGGGCT ACCAGTACTA TGACTGCCTA CAGGGTGGCT 
     1261 TCGTGCGCGG CCGCGTGCCC GCCGGTCAGT CCTATTTTGT GGACTTCGGG AGCACTGAGT 
     1321 GCTCCTGCCC ACCAGGCGGC GGCAAGATCA GCTGCCAGTT CATGCTGTGC CCGGAGCTGC 
     1381 CGCCCAACTG CATCGAGGCT GTAGTGGTGG CTGACAGCTG CCCACAGTGC GGCCAGGTGG 
     1441 GCTGCGTCCA CGCGGGCCAC AAGTACGCCG CTGGCCACAC TGTTCACCTG CCGCCCTGCC 
     1501 GGGCCTGCCA CTGCCCTGAC GCCGGTGGAG AGCTCATCTG CTACCAGCTC CCCGGTTGCC 
     1561 ACGGGAACTT CTCAGATGCC GAGGAGGGTG ACCCCGAGCG ACACTACGAA GACCCCTACA 
     1621 GCTATGACCA GGAGGTGGCC GAGGTGGAAG CAGCAACAGC CCTGGGGGGT GAGGTCCAGG 
     1681 CGGGTGCAGT CCAGGCAGGC GCAGGGGGCC CCCCAGCTGC TCTGGGAGGT GGGAGTCAGC 
     1741 CACTGTCCAC CATCCAGGCA CCCCCCTGGC CAGCTGTCCT CCCCAGGCCC ACAGCGGCTG 
     1801 CTGCCCTGGG TCCCCCAGCC CCAGTGCAGG CCAAAGCTAG GAGAGTGACC GAGGACAGTG 
     1861 AGGAGGAAGA AGAGGAGGAG GAGGAGAGAG AGGAAATGGC TGTCACTGAG CAGCTGGCAG 
     1921 CAGGTGGCCA CAGGGGGCTG GATGGGCTGC CCACTACAGC CCCAGCTGGA CCCAGTCTTC 
     1981 CTATCCAGGA GGAGAGGGCA GAAGCTGGGG CAAGGGCAGA AGCTGGGGCA AGGCCTGAAG 
     2041 AGAACCTCAT CCTGGATGCC CAAGCCACGT CCCGCAGCAC TGGGCCGGAG GGCGTGACGC 
     2101 ATGCACCGAG CCTGGGCAAG GCTGCTCTCG TCCCAACTCA GGCCGTGCCT GGCTCTCCCA 
     2161 GGGACCCAGT CAAGCCCAGC CCCCACAACA TCCTGTCCAC ATCACTGCCT GATGCAGCCT 
     2221 GGATCCCACC CACCCGAGAA GTGCCCAGGA AGCCGCAAGT TCTGCCCCAT TCCCACGTGG 
     2281 AGGAGGACAC AGACCCCAAC TCTGTCCATT CTATCCCCAG AAGTAGCCCT GAAGGCTCCA 
     2341 CCAAGGACCT GATCGAGACT TGCTGCGCAG CCGGACAGCA GTGGGCCATT GACAATGACG 
     2401 AGTGCCTGGA GATCCCTGAG AGTGGCACTG AGGACAACGT CTGCAGGACA GCCCAGAGGC 
     2461 ACTGCTGTGT CTCCTACTTG CAGGAGAAGA GCTGCATGGC CGGCGTCCTG GGAGCCAAGG 
     2521 AGGGTGAGAC CTGTGGGGCT GAGGACAACG ACAGCTGCGG CATCTCCCTG TACAAGCAAT 
     2581 GCTGTGACTG CTGTGGCCTG GGCCTCCGCG TGCGGGCCGA GGGCCAGTCG TGTGAGTCCA 
     2641 ATCCTAACCT GGGCTATCCC TGCAATCATG TCATGCTCTC CTGCTGTGAG GGTGAAGAGC 
     2701 CTCTCATAGT ACCTGAGGTT CGCCGACCTC CAGAGCCCGC AGCTGCACCA CGGAGAGTTT 
     2761 CAGAGGCAGA GATGGCGGGC CGAGAGGCCC TGTCACTGGG CACAGAGGCC GAGCTGCCGA 
     2821 ACAGCCTGCC GGGCGATGAC CAGGATGAGT GCCTTCTCCT CCCGGGAGAG CTGTGCCAGC 
     2881 ACCTTTGCAT CAATACTGTG GGTTCTTACC ACTGTGCCTG CTTTCCTGGC TTCTCACTGC 
     2941 AGGACGATGG CCGCACTTGC CGCCCAGAGG GTCACCCTCC ACAGCCGGAA GCCCCACAGG 
     3001 AGCCTGCACT GAAGTCAGAA TTTTCCCAGG TGGCCTCTAA CACCATCCCG CTGCCACTGC 
     3061 CGCAGCCCAA TACCTGCAAA GACAATGGAC CCTGCAAGCA GGTGTGCAGC ACTGTTGGGG 
     3121 GCTCAGCCAT ATGCTCCTGT TTTCCCGGCT ATGCCATCAT GGCGGATGGC GTGTCCTGTG 
     3181 AAGACATCAA CGAGTGTGTG ACGGACCTGC ACACGTGCAG CCGGGGCGAG CACTGTGTGA 
     3241 ACACACTGGG CTCCTTCCAC TGCTACAAGG CACTCACCTG TGAGCCAGGC TATGCCCTCA 
     3301 AGGATGGCGA GTGCGAAGAC GTGGATGAGT GTGCGATGGG CACGCACACC TGCCAGCCGG 
     3361 GCTTCTTGTG CCAGAACACC AAGGGCTCCT TCTACTGCCA GGCCAGGCAG CGCTGCATGG 
     3421 ATGGCTTCCT GCAGGATCCT GAAGGCAACT GTGTGGACAT CAACGAGTGC ACGTCACTGT 
     3481 CCGAGCCATG TCGGCCAGGC TTCAGCTGCA TCAACACGGT GGGCTCCTAC ACGTGCCAGA 
     3541 GGAACCCGCT GATCTGCGCG CGCGGCTACC ACGCCAGCGA TGATGGGGCC AAGTGTGTGG 
     3601 ACGTGAATGA GTGTGAGACA GGTGTGCACC GCTGCGGTGA GGGCCAAGTG TGCCACAACC 
     3661 TCCCTGGCTC CTACCGCTGT GACTGCAAAG CCGGCTTTCA GCGGGATGCC TTCGGCCGGG 
     3721 GCTGCATCGA CGTGAATGAG TGCTGGGCCT CGCCAGGCCG CCTGTGCCAG CACACGTGTG 
     3781 AGAACACACT CGGCTCCTAC CGCTGTTCCT GCGCCTCCGG GTTCCTGCTA GCAGCGGACG 
     3841 GCAAGCGCTG TGAAGACGTG AATGAGTGTG AGGCCCAGCG CTGCAGCCAG GAGTGTGCCA 
     3901 ACATCTATGG CTCCTACCAG TGCTACTGCC GCCAGGGCTA CCAGCTGGCT GAGGATGGGC 
     3961 ACACCTGCAC AGACATCGAC GAGTGTGCTC AAGGCGCCGG CATCCTCTGC ACCTTCCGCT 
     4021 GTCTCAACGT GCCAGGGAGC TACCAGTGTG CATGCCCTGA GCAGGGCTAC ACCATGACGG 
     4081 CCAACGGGAG GTCCTGCAAG GACGTGGATG AGTGTGCACT GGGTACCCAC AACTGTTCCG 
     4141 AGGCTGAGAC CTGCCACAAC ATCCAGGGTA GCTTCCGCTG CCTGCGCTTA GAGTGTCCTC 
     4201 CCAACTATGT CCAAGTCTCC AAAACGAAGT GCGAGCGCAC CACGTGCCAT GACTTCCTGG 
     4261 AGTGCCAGAA CTCGCCAGCG CGCATCACGC ACTACCAGCT CAACTTCCAG ACGGGCCTCC 
     4321 TGGTGCCTGC GCATATCTTC CGCATTGGCC CCGCGCCAGC CTTCACGGGG GACACCATCG 
     4381 CCCTGAACAT CATCAAGGGC AATGAGGAGG GCTACTTTGG CACGCGCAGG CTCAATGCCT 
     4441 ACACGGGTGT GGTCTACCTG CAGCGGGCCG TGCTGGAGCC CCGGGACTTT GCCCTGGACG 
     4501 TGGAGATGAA GCTCTGGAGG CAGGGCTCCG TCACCACCTT CCTGGCCAAG ATGCACATCT 
     4561 TCTTCACCAC CTTTGCCCTG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4621 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4681 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4741 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4801 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4861 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4921 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4981 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5041 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5101 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5161 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5221 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5281 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5341 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5401 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5461 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5521 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5581 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5641 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5701 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5761 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5821 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5881 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5941 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6001 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6061 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6121 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6181 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6241 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6301 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6361 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6421 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6481 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6541 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6601 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6661 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6721 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6781 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6841 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6901 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6961 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7021 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7081 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7141 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7201 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7261 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7321 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7381 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7441 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7501 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7561 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7621 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7681 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7741 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7801 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7861 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7921 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7981 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8041 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8101 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8161 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8221 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8281 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8341 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8401 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//