LOCUS       RC208189                8439 bp    DNA     circular  SYN 
DEFINITION  PALB2 (Myc-DDK-tagged)-Human partner and localizer of BRCA2 (PALB2)
ACCESSION   NM_024675
VERSION     NM_024675.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4596..4687 
                     /label="Myc-DDK"  
     misc_feature    4717..5302 
                     /label="PolyA signal"  
     misc_feature    4774..4793 
                     /label="Restriction Enzyme"  
     misc_feature    5578..6250 
                     /label="ColE1"  
     misc_feature    6680..7474 
                     /label="Kan/Neo"  
     misc_feature    7509..7811 
                     /label="SV40 ori"  
     misc_feature    7897..8374 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4578..4584 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4578 
                     /label="PALB2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACGAGCCT CCCGGGAAGC CCCTCAGCTG TGAGGAGAAG GAAAAGTTAA 
     1081 AGGAGAAATT AGCATTCTTG AAAAGGGAAT ACAGCAAGAC ACTAGCCCGC CTTCAGCGTG 
     1141 CCCAAAGAGC TGAAAAGATT AAGCATTCTA TTAAGAAAAC AGTAGAAGAA CAAGATTGTT 
     1201 TGTCTCAGCA GGATCTCTCA CCGCAGCTAA AACACTCAGA ACCTAAAAAT AAAATATGTG 
     1261 TTTATGACAA GTTACACATC AAAACCCATC TTGATGAAGA AACTGGAGAA AAGACATCTA 
     1321 TCACACTTGA TGTTGGGCCT GAGTCCTTTA ACCCTGGAGA TGGCCCAGGA GGATTACCTA 
     1381 TACAAAGAAC AGATGACACC CAAGAACATT TTCCCCACAG GGTCAGTGAC CCTAGTGGTG 
     1441 AGCAAAAGCA GAAGCTGCCA AGCAGAAGAA AGAAGCAGCA GAAGAGGACA TTTATTTCAC 
     1501 AGGAGAGAGA CTGTGTCTTT GGCACTGATT CACTCAGATT GTCTGGGAAA AGACTAAAGG 
     1561 AACAGGAAGA AATCAGTAGC AAAAATCCTG CTAGATCACC AGTAACTGAA ATAAGAACTC 
     1621 ACCTTTTAAG TCTTAAATCT GAACTTCCAG ATTCTCCAGA ACCAGTTACA GAAATTAATG 
     1681 AAGACAGTGT ATTAATTCCA CCAACTGCCC AACCAGAAAA AGGTGTTGAT ACATTCCTAA 
     1741 GAAGACCTAA TTTCACCAGG GCGACTACAG TTCCTTTACA GACTCTATCA GATAGCGGTA 
     1801 GTAGTCAGCA CCTTGAACAC ATTCCTCCTA AAGGTAGCAG TGAACTTACT ACTCACGACC 
     1861 TAAAAAACAT TAGATTTACT TCACCTGTAA GTTTGGAGGC ACAAGGCAAA AAAATGACTG 
     1921 TCTCTACAGA TAACCTCCTT GTAAATAAAG CTATAAGTAA AAGTGGCCAA CTGCCCACAA 
     1981 GTTCTAATTT AGAGGCAAAT ATTTCATGTT CTCTAAATGA ACTCACCTAC AATAACTTAC 
     2041 CAGCAAATGA AAACCAAAAC TTAAAAGAAC AAAATCAAAC AGAGAAATCT TTAAAATCTC 
     2101 CCAGTGACAC TCTTGATGGC AGGAATGAAA ATCTTCAGGA AAGTGAGATT CTAAGTCAAC 
     2161 CTAAGAGTCT TAGCCTGGAA GCAACCTCTC CTCTTTCTGC AGAAAAACAT TCTTGCACAG 
     2221 TGCCTGAAGG CCTTCTGTTT CCTGCAGAAT ATTATGTTAG AACAACACGA AGCATGTCCA 
     2281 ATTGCCAGAG GAAAGTAGCC GTGGAGGCTG TCATTCAGAG TCATTTGGAT GTCAAGAAAA 
     2341 AAGGGTTTAA AAATAAAAAT AAGGATGCAA GTAAAAATTT AAACCTTTCC AATGAGGAAA 
     2401 CTGACCAAAG TGAAATTAGG ATGTCTGGCA CATGCACAGG ACAACCAAGT TCAAGAACCT 
     2461 CTCAGAAACT TCTCTCATTA ACTAAAGTCA GCTCTCCCGC TGGGCCCACT GAAGATAATG 
     2521 ACTTGTCTAG GAAGGCAGTT GCCCAAGCAC CTGGTAGAAG ATACACAGGA AAAAGAAAAT 
     2581 CAGCCTGCAC CCCAGCATCA GATCATTGTG AACCACTTTT GCCAACTTCT AGCCTGTCGA 
     2641 TTGTTAACAG GTCCAAGGAA GAAGTCACCT CACACAAATA TCAGCACGAA AAATTATTTA 
     2701 TTCAAGTGAA AGGGAAGAAA AGTCGTCATC AAAAAGAGGA TTCCCTTTCT TGGAGTAATA 
     2761 GTGCTTATTT ATCCTTGGAT GATGATGCTT TCACGGCTCC ATTTCATAGG GATGGAATGC 
     2821 TGAGTTTAAA GCAACTACTG TCTTTTCTCA GTATCACAGA CTTTCAGTTA CCTGATGAAG 
     2881 ACTTTGGACC TCTTAAGCTT GAAAAAGTGA AGTCCTGCTC AGAAAAACCA GTGGAGCCCT 
     2941 TTGAGTCAAA AATGTTTGGA GAGAGACATC TTAAAGAGGG AAGCTGTATT TTTCCAGAGG 
     3001 AACTGAGTCC TAAACGCATG GATACAGAAA TGGAGGACTT AGAAGAGGAC CTTATTGTTC 
     3061 TACCAGGAAA ATCACATCCC AAAAGGCCAA ACTCGCAAAG CCAGCATACA AAGACGGGCC 
     3121 TTTCTTCATC CATATTACTT TATACTCCTT TAAATACGGT TGCGCCTGAT GATAATGACA 
     3181 GGCCTACCAC AGACATGTGT TCACCTGCTT TCCCCATCTT AGGTACTACT CCAGCCTTTG 
     3241 GCCCTCAAGG CTCCTATGAA AAAGCATCTA CAGAAGTTGC TGGACGAACT TGCTGCACAC 
     3301 CCCAACTTGC TCATTTGAAA GACTCAGTCT GTCTTGCCAG TGATACTAAA CAATTCGACA 
     3361 GTTCAGGCAG CCCAGCAAAA CCACATACCA CCCTGCAAGT GTCAGGCAGG CAAGGACAAC 
     3421 CTACCTGTGA CTGTGACTCT GTCCCGCCAG GAACACCTCC ACCCATTGAG TCATTCACTT 
     3481 TTAAAGAAAA TCAGCTCTGT AGAAACACAT GCCAGGAGCT GCATAAACAT TCCGTCGAAC 
     3541 AGACTGAAAC AGCAGAGCTT CCTGCTTCTG ATAGCATAAA CCCAGGCAAC CTACAATTGG 
     3601 TTTCAGAGTT AAAGAATCCT TCAGGTTCCT GTTCCGTAGA TGTGAGTGCC ATGTTTTGGG 
     3661 AAAGAGCCGG TTGTAAAGAG CCATGTATCA TAACTGCTTG CGAAGATGTA GTTTCTCTTT 
     3721 GGAAAGCTCT GGATGCTTGG CAGTGGGAAA AACTTTATAC CTGGCACTTC GCAGAGGTTC 
     3781 CAGTATTACA GATAGTTCCA GTGCCTGATG TGTATAATCT CGTGTGTGTA GCTTTGGGAA 
     3841 ATTTGGAAAT CAGAGAGATC AGGGCATTGT TTTGTTCCTC TGATGATGAA AGTGAAAAGC 
     3901 AAGTACTACT GAAGTCTGGA AATATAAAAG CTGTGCTTGG CCTGACAAAG AGGAGGCTAG 
     3961 TTAGTAGCAG TGGGACCCTT TCTGATCAAC AAGTAGAAGT CATGACGTTT GCAGAAGATG 
     4021 GAGGAGGCAA AGAAAACCAA TTTTTGATGC CCCCTGAGGA GACTATACTA ACTTTTGCTG 
     4081 AGGTCCAAGG GATGCAAGAA GCTCTGCTTG GTACTACTAT TATGAACAAC ATTGTTATTT 
     4141 GGAATTTAAA AACTGGTCAA CTCCTGAAAA AGATGCACAT TGATGATTCT TACCAAGCTT 
     4201 CAGTCTGTCA CAAAGCCTAT TCTGAAATGG GGCTTCTCTT TATTGTCCTG AGTCATCCCT 
     4261 GTGCCAAAGA GAGTGAGTCG TTGCGAAGCC CTGTGTTTCA GCTCATTGTG ATTAACCCTA 
     4321 AGACGACTCT CAGCGTGGGT GTGATGCTGT ACTGTCTTCC TCCAGGGCAG GCTGGCAGGT 
     4381 TCCTGGAAGG TGACGTGAAA GATCACTGTG CAGCAGCAAT CTTGACTTCT GGAACAATTG 
     4441 CCATTTGGGA CTTACTTCTC GGTCAGTGTA CTGCCCTCCT CCCACCTGTC TCTGACCAAC 
     4501 ATTGGTCTTT TGTGAAATGG TCGGGTACAG ACTCTCATTT GCTGGCTGGA CAAAAAGATG 
     4561 GAAATATATT TGTATACCAC TATTCAACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4621 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4681 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4741 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4801 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4861 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4921 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4981 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     5041 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5101 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5161 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5221 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5281 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5341 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5401 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5461 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5521 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5581 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5641 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5701 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5761 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5821 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5881 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5941 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     6001 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6061 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6121 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6181 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6241 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6301 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6361 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6421 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6481 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6541 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6601 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6661 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6721 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6781 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6841 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6901 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6961 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     7021 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7081 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7141 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7201 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7261 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7321 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7381 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7441 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7501 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7561 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7621 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7681 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7741 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7801 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7861 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7921 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7981 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     8041 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     8101 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8161 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8221 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8281 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8341 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8401 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//