LOCUS       RC208116                7209 bp    DNA     circular  SYN 
DEFINITION  PANK4 (Myc-DDK-tagged)-Human pantothenate kinase 4 (PANK4)
ACCESSION   NM_018216
VERSION     NM_018216.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3364..3455 
                     /label="Myc-DDK"  
     misc_feature    3485..4070 
                     /label="PolyA signal"  
     misc_feature    3542..3561 
                     /label="Restriction Enzyme"  
     misc_feature    4346..5018 
                     /label="ColE1"  
     misc_feature    5448..6242 
                     /label="Kan/Neo"  
     misc_feature    6277..6579 
                     /label="SV40 ori"  
     misc_feature    6665..7142 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3339..3346 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3339 
                     /label="PANK4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGAGTGT GGAGCGAGCG GCAGCGGGAG CAGCGGGGAC AGTCTGGACA 
     1081 AGAGCATCAC GCTGCCCCCC GACGAGATCT TCCGCAACCT GGAGAACGCC AAGCGCTTCG 
     1141 CCATCGACAT AGGCGGGTCG TTAACCAAGC TGGCCTACTA TTCAACGGTA CAGCACAAAG 
     1201 TCGCCAAGGT GCGGTCTTTC GACCACTCCG GAAAGGACAC AGAACGTGAA CATGAGCCGC 
     1261 CCTATGAGAT TTCAGTTCAA GAAGAGATCA CTGCTCGACT GCACTTCATT AAGTTTGAGA 
     1321 ATACCTACAT CGAAGCCTGC CTGGACTTCA TCAAAGACCA TCTCGTCAAC ACAGAGACCA 
     1381 AGGTCATCCA GGCGACCGGG GGCGGGGCCT ACAAGTTCAA GGACCTCATC GAAGAGAAGC 
     1441 TGCGGCTGAA AGTCGACAAG GAGGACGTGA TGACGTGCCT GATTAAGGGG TGCAACTTCG 
     1501 TGCTCAAGAA CATCCCCCAT GAGGCCTTCG TGTACCAGAA GGATTCCGAC CCTGAGTTCC 
     1561 GGTTCCAGAC CAACCACCCC CACATTTTCC CCTATCTTCT TGTCAATATC GGCTCTGGAG 
     1621 TCTCCATCGT GAAGGTGGAG ACGGAGGACA GGTTCGAGTG GGTCGGCGGC AGCTCCATTG 
     1681 GAGGCGGCAC CTTCTGGGGG CTTGGCGCTC TGCTCACCAA AACGAAGAAG TTTGACGAGC 
     1741 TCCTGCACCT GGCCTCGAGG GGCCAGCACA GCAATGTGGA CATGCTGGTG CGGGACGTCT 
     1801 ACGGCGGCGC CCACCAGACT CTCGGGCTGA GCGGGAACCT CATCGCCAGC AGCTTCGGGA 
     1861 AGTCGGCCAC CGCCGACCAA GAGTTCTCCA AAGAAGACAT GGCGAAGAGC CTGCTGCACA 
     1921 TGATCAGCAA CGACATTGGG CAGCTGGCCT GCCTCCACGC ACGGCTGCAC AGCCTGGACC 
     1981 GCGTGTACTT TGGAGGCTTC TTTATCCGGG GCCACCCCGT GACCATGCGC ACCATCACCT 
     2041 ATAGCATCAA CTTCTTCTCC AAGGGGGAAG TGCAGGCGCT GTTTCTGAGG CACGAAGGCT 
     2101 ACCTGGGAGC CATCGGAGCG TTCCTGAAAG GAGCTGAGCA GGACAATCCT AACCAGTACA 
     2161 GCTGGGGAGA GAACTATGCA GGCAGCTCCG GGCTGATGAG TGCATCACCC GAGCTCGGCC 
     2221 CGGCGCAGCG GGCGCGGAGT GGCACTTTTG ACTTGCTGGA AATGGACCGG CTGGAGAGGC 
     2281 CACTGGTTGA CCTGCCGCTC CTCCTGGACC CGCCCTCCTA CGTGCCCGAC ACGGTGGACC 
     2341 TCACCGATGA CGCTCTGGCC CGAAAATACT GGCTCACCTG CTTTGAGGAG GCCCTGGACG 
     2401 GGGTAGTGAA GCGCGCAGTG GCGAGCCAGC CAGACTCTGT GGATGCAGCC GAGAGGGCGG 
     2461 AGAAGTTCCG GCAGAAGTAC TGGAACAAGC TTCAGACCCT GAGGCAGCAG CCCTTCGCCT 
     2521 ATGGGACCCT GACCGTGCGC AGCCTGCTGG ACACCAGGGA GCACTGTCTG AACGAGTTCA 
     2581 ACTTCCCGGA TCCCTACTCC AAAGTGAAGC AGCGGGAGAA TGGCGTGGCG CTGAGGTGCT 
     2641 TCCCCGGGGT CGTGCGCTCC CTGGACGCGC TGGGCTGGGA GGAACGGCAG CTGGCGCTGG 
     2701 TGAAAGGCCT CCTGGCGGGG AATGTCTTCG ACTGGGGGGC CAAAGCCGTG TCTGCTGTCC 
     2761 TTGAATCCGA CCCCTACTTT GGGTTTGAAG AAGCAAAGAG GAAGTTACAA GAAAGACCCT 
     2821 GGCTCGTGGA TTCCTACAGC GAGTGGCTTC AGAGATTAAA GGGGCCCCCT CATAAATGTG 
     2881 CCTTAATTTT CGCAGATAAC AGTGGAATAG ACATCATTTT GGGAGTCTTC CCCTTTGTCA 
     2941 GGGAGCTACT CCTTAGAGGG ACAGAGGTCA TCCTGGCGTG CAACTCAGGC CCCGCCCTGA 
     3001 ACGACGTGAC CCACAGCGAG TCCCTCATCG TGGCAGAGCG TATTGCGGGC ATGGACCCTG 
     3061 TCGTGCACTC TGCGCTCCAG GAAGAGAGGC TGCTGCTGGT GCAGACGGGC TCCAGCTCCC 
     3121 CGTGCCTCGA CCTCAGCCGC CTGGATAAGG GGCTGGCCGC ACTGGTGCGG GAGCGTGGCG 
     3181 CGGATCTGGT GGTCATCGAG GGCATGGGCC GTGCTGTCCA CACAAACTAC CACGCAGCCC 
     3241 TGCGCTGCGA GAGCCTCAAG CTGGCCGTCA TCAAGAACGC GTGGCTGGCC GAGCGGCTGG 
     3301 GCGGCCGGCT CTTCAGCGTC ATCTTCAAGT ACGAGGTCCC AGCCGAGAGC GGACCGACGC 
     3361 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     3421 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     3481 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     3541 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     3601 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     3661 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     3721 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     3781 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     3841 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     3901 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     3961 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4021 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4081 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     4141 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     4201 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     4261 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     4321 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     4381 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     4441 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     4501 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     4561 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     4621 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     4681 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     4741 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     4801 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     4861 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     4921 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     4981 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     5041 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     5101 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     5161 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     5221 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     5281 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     5341 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     5401 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     5461 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     5521 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     5581 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     5641 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     5701 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     5761 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     5821 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     5881 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     5941 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     6001 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     6061 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     6121 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     6181 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     6241 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     6301 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     6361 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     6421 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     6481 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     6541 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6601 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     6661 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     6721 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     6781 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     6841 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     6901 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     6961 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     7021 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     7081 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     7141 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     7201 GCGAATTTT
//