LOCUS       RC208109                6648 bp    DNA     circular  SYN 
DEFINITION  SENP2 (Myc-DDK-tagged)-Human SUMO1/sentrin/SMT3 specific peptidase 2 (SENP2)
ACCESSION   NM_021627
VERSION     NM_021627.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2805..2896 
                     /label="Myc-DDK"  
     misc_feature    2926..3511 
                     /label="PolyA signal"  
     misc_feature    2983..3002 
                     /label="Restriction Enzyme"  
     misc_feature    3787..4459 
                     /label="ColE1"  
     misc_feature    4889..5683 
                     /label="Kan/Neo"  
     misc_feature    5718..6020 
                     /label="SV40 ori"  
     misc_feature    6106..6583 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2787..2793 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2787 
                     /label="SENP2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTACAGATGG CTGGTTAGGA TTCTCGGCAC CATTTTCCGT TTCTGCGACC 
     1081 GGTCGGTGCC CCCTGCCCGG GCCCTCCTGA AGAGGCGGCG CTCAGACAGC ACTCTGTTTT 
     1141 CTACAGTGGA CACTGATGAA ATACCAGCCA AAAGACCAAG ATTAGATTGC TTTATTCACC 
     1201 AAGTGAAAAA CAGTCTCTAC AATGCTGCCA GCTTATTTGG ATTCCCATTC CAGCTGACCA 
     1261 CAAAGCCCAT GGTAACTTCT GCTTGTAATG GAACACGGAA TGTGGCCCCT TCAGGAGAGG 
     1321 TATTTTCGAA CTCTTCATCT TGTGAACTGA CAGGTTCTGG ATCCTGGAAC AACATGCTGA 
     1381 AACTGGGTAA TAAATCTCCT AATGGAATAA GTGACTATCC AAAGATCAGA GTGACAGTTA 
     1441 CCCGAGATCA GCCACGCAGA GTCCTGCCTT CCTTTGGTTT TACTTTGAAC TCAGAAGGCT 
     1501 GTAATAGAAG ACCAGGTGGC CGTCGCCATA GCAAAGGTAA TCCAGAGAGT TCTTTAATGT 
     1561 GGAAACCTCA GGAACAGGCT GTAACAGAGA TGATTTCTGA AGAGAGTGGC AAGGGTCTGA 
     1621 GGCGTCCCCA TTGTACTGTG GAGGAGGGTG TTCAAAAAGA GGAAAGAGAG AAGTACCGAA 
     1681 AGTTATTGGA ACGACTTAAA GAAAGTGGTC ATGGAAACTC TGTCTGTCCT GTAACTTCAA 
     1741 ATTATCACAG TTCTCAAAGA AGTCAGATGG ACACATTAAA GACCAAAGGC TGGGGGGAAG 
     1801 AGCAAAATCA CGGAGTCAAA ACAACTCAGT TTGTTCCAAA ACAATATAGA CTTGTTGAAA 
     1861 CAAGGGGACC TCTATGTTCA TTGAGAAGTG AAAAGAGGTG TTCAAAGGGG AAAATTACTG 
     1921 ATACAGAGAC GATGGTCGGA ATCAGATTTG AAAATGAAAG TAGGAGGGGA TACCAACTGG 
     1981 AGCCTGACCT ATCAGAAGAA GTGTCGGCCC GACTCCGCCT GGGCAGTGGA AGCAATGGCT 
     2041 TACTCAGGAG GAAAGTGTCA ATAATTGAGA CAAAGGAAAA GAATTGCTCA GGCAAAGAGA 
     2101 GGGACAGAAG AACGGACGAT CTCCTTGAAC TTACAGAGGA CATGGAAAAG GAAATCAGTA 
     2161 ATGCCCTAGG CCATGGCCCA CAGGATGAAA TCCTAAGTAG TGCTTTCAAA TTGCGAATTA 
     2221 CTCGAGGAGA TATTCAGACA TTAAAGAACT ATCACTGGCT CAATGATGAA GTCATTAATT 
     2281 TTTACATGAA TCTTCTGGTG GAAAGAAATA AAAAGCAAGG CTATCCAGCA CTTCATGTAT 
     2341 TCAGTACTTT CTTCTATCCT AAATTAAAGT CTGGGGGTTA CCAAGCAGTG AAACGATGGA 
     2401 CCAAAGGGGT AAATCTCTTT GAACAAGAAA TTATTCTGGT GCCTATTCAT CGGAAGGTAC 
     2461 ATTGGAGCCT GGTGGTGATT GACCTAAGAA AAAAGTGTCT TAAATATCTG GATTCTATGG 
     2521 GACAAAAGGG CCACAGGATC TGTGAGATTC TCCTTCAGTA TTTACAGGAT GAAAGTAAGA 
     2581 CCAAAAGAAA TAGTGATCTG AATCTTTTAG AGTGGACCCA TCACAGCATG AAACCACACG 
     2641 AGATTCCTCA ACAGCTGAAT GGGAGTGATT GTGGAATGTT TACTTGTAAA TATGCAGATT 
     2701 ATATTTCTAG GGACAAACCT ATCACATTTA CTCAGCACCA GATGCCTCTC TTCCGGAAGA 
     2761 AGATGGTGTG GGAAATCCTT CATCAGCAGT TGCTGACGCG TACGCGGCCG CTCGAGCAGA 
     2821 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     2881 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     2941 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     3001 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     3061 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     3121 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     3181 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     3241 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     3301 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     3361 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     3421 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     3481 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     3541 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     3601 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     3661 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     3721 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     3781 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     3841 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     3901 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     3961 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     4021 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     4081 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     4141 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     4201 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     4261 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     4321 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     4381 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     4441 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     4501 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     4561 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     4621 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     4681 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     4741 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     4801 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     4861 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     4921 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     4981 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     5041 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     5101 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     5161 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     5221 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     5281 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     5341 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     5401 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     5461 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     5521 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     5581 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     5641 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     5701 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     5761 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     5821 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     5881 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     5941 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     6001 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     6061 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     6121 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     6181 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     6241 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     6301 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     6361 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     6421 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     6481 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     6541 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     6601 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//