LOCUS       RC208092                8166 bp    DNA     circular  SYN 
DEFINITION  RAPGEF1 (Myc-DDK-tagged)-Human Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant 2
ACCESSION   NM_198679
VERSION     NM_198679.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4323..4414 
                     /label="Myc-DDK"  
     misc_feature    4444..5029 
                     /label="PolyA signal"  
     misc_feature    4501..4520 
                     /label="Restriction Enzyme"  
     misc_feature    5305..5977 
                     /label="ColE1"  
     misc_feature    6407..7201 
                     /label="Kan/Neo"  
     misc_feature    7236..7538 
                     /label="SV40 ori"  
     misc_feature    7624..8101 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4305..4311 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4305 
                     /label="RAPGEF1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCAATGCT ATTGAAAAAC AGAAGCCCTT GAAACGAAGT CATCTGTACC 
     1081 CCTGGAAACA AGACTCTCAG CGTTCTCATC TCTCTTCCTT CACCATGAAG CTGATGGACA 
     1141 AATTCCACTC ACCCAAAATC AAGAGAACGC CATCAAAGAA GGGAAAACCA GCTGAGGTGT 
     1201 CCGTAAAGAT TCCAGAGAAG CCTGTGAACA AAGAGGCAAC AGACAGATTT CTACCAGAGG 
     1261 GCTACCCTCT CCCCTTGGAT CTGGAGCAGC AGGCAGTAGA ATTTATGTCC ACCAGTGCTG 
     1321 TGGCTTCCAG GTCTCAAAGG CAGAAGAACC TGAGCTGGCT GGAGGAGAAA GAGAAGGAAG 
     1381 TTGTCAGTGC CCTGCGCTAC TTTAAGACCA TTGTGGACAA AATGGCAATT GATAAGAAGG 
     1441 TACTGGAGAT GCTTCCAGGG TCAGCCAGCA AGGTGCTGGA GGCCATCTTA CCCCTGGTGC 
     1501 AGAACGATCC TCGAATTCAG CACAGCTCAG CCCTCTCTTC CTGCTATAGC CGAGTGTACC 
     1561 AAAGCCTCGC CAACCTCATT CGCTGGTCTG ACCAAGTGAT GCTGGAAGGC GTGAACTCAG 
     1621 AAGACAAGGA GATGGTGACG ACTGTGAAGG GGGTCATCAA GGCTGTGCTG GATGGAGTGA 
     1681 AGGAGCTGGT CAGGCTCACC ATCGAGAAGC AGGGACGTCC GTCTCCGACG AGCCCCGTGA 
     1741 AGCCCAGTTC CCCTGCCAGC AAGCCTGATG GCCCAGCAGA GCTCCCCCTG ACAGACCGCG 
     1801 AGGTAGAGAT CCTAAACAAG ACGACTGGGA TGTCACAGTC AACTGAGCTC CTCCCAGATG 
     1861 CCACGGATGA AGAGGTCGCG CCCCCCAAGC CTCCTCTGCC TGGCATTCGG GTGGTTGATA 
     1921 ATAGTCCTCC ACCAGCATTG CCACCCAAGA AAAGACAGTC GGCGCCGTCC CCTACCCGAG 
     1981 TGGCTGTGGT GGCCCCCATG AGCCGAGCCA CCAGTGGCTC CAGTTTGCCT GTTGGAATCA 
     2041 ATAGGCAGGA TTTTGATGTT GACTGTTACG CACAGAGGCG ACTGTCAGGA GGCAGCCACT 
     2101 CATATGGTGG AGAGTCGCCC CGCCTCTCCC CCTGCAGCAG CATAGGCAAG CTCAGCAAGT 
     2161 CAGACGAGCA GCTGTCCTCT CTGGACAGGG ACAGTGGGCA GTGCTCCCGG AACACAAGCT 
     2221 GTGAAACACT AGACCACTAT GATCCCGACT ATGAATTCCT CCAGCAAGAC CTCTCTAACG 
     2281 CAGACCAGAT ACCTCAGCAG ACGGCCTGGA ACCTTAGCCC GTTGCCAGAG TCTTTGGGGG 
     2341 AGTCTGGGTC TCCATTTCTT GGCCCTCCTT TCCAGCTGCC TCTTGGCGGC CATCCCCAGC 
     2401 CAGACGGACC TCTGGCCCCA GGGCAGCAGA CAGATACGCC ACCTGCTCTC CCCGAGAAGA 
     2461 AGCGCAGGAG CGCAGCCTCC CAGACGGCGG ACGGCTCTGG CTGCAGGGTG TCCTACGAGC 
     2521 GGCATCCCTC GCAGTATGAC AACATCTCTG GGGAGGACCT GCAGAGCACA GCCCCGATCC 
     2581 CATCCGTCCC CTACGCGCCC TTTGCTGCTA TTCTGCCCTT TCAGCATGGA GGTTCCTCAG 
     2641 CCCCTGTCGA ATTTGTGGGT GATTTTACTG CTCCTGAGTC AACCGGTGAC CCAGAAAAAC 
     2701 CACCTCCTCT ACCAGAGAAG AAAAACAAAC ACATGCTGGC CTACATGCAG TTGCTGGAGG 
     2761 ACTACTCGGA GCCGCAGCCC TCTATGTTCT ACCAGACGCC ACAGAACGAG CACATCTACC 
     2821 AGCAGAAGAA CAAGCTCCTC ATGGAGGTAT ACGGCTTCAG CGACTCCTTC AGTGGGGTGG 
     2881 ACTCCGTGCA GGAGCTGGCC CCGCCGCCCG CCCTACCCCC CAAGCAGCGG CAGCTGGAGC 
     2941 CACCGGCTGG GAAAGACGGA CATCCCAGAG ATCCCTCAGC GGTCAGCGGC GTCCCTGGGA 
     3001 AGGACAGCAG AGACGGCAGT GAGAGGGCCC CAAAGTCACC AGATGCTCTG GAGTCGGCTC 
     3061 AGTCGGAGGA GGAAGTGGAC GAGCTGTCCC TCATTGACCA CAACGAAATT ATGTCCAGGC 
     3121 TGACGCTCAA GCAGGAGGGT GATGACGGGC CGGACGTCCG CGGAGGATCT GGGGACATCT 
     3181 TACTGGTCCA TGCTACTGAG ACTGACAGGA AAGATTTGGT GTTGTACTGC GAGGCATTCC 
     3241 TGACCACCTA CAGGACCTTC ATCTCCCCAG AGGAGCTCAT CAAGAAGCTG CAGTACAGAT 
     3301 ATGAGAAATT CTCTCCCTTT GCCGACACAT TCAAGAAGCG CGTCAGCAAG AACACGTTCT 
     3361 TCGTGCTGGT ACGGGTGGTG GATGAGCTCT GCCTGGTGGA GTTGACAGAA GAGATCCTGA 
     3421 AGCTGCTGAT GGAACTGGTC TTCCGCCTGG TGTGCAATGG GGAGCTGAGC CTGGCCCGTG 
     3481 TGCTCCGGAA GAACATCCTG GACAAGGTGG ACCAGAAGAA GCTACTCAGG TGTGCCACCT 
     3541 CCAGCCAGCC CCTGGCAGCC CGGGGGGTAG CAGCCAGGCC GGGGACCTTG CACGACTTTC 
     3601 ACAGCCATGA GATAGCGGAG CAGCTAACGC TGCTGGATGC TGAGCTCTTC TATAAAATAG 
     3661 AGATTCCTGA GGTTTTGCTT TGGGCAAAAG AGCAGAATGA GGAGAAGAGC CCCAACTTGA 
     3721 CCCAGTTCAC GGAGCACTTC AACAACATGT CCTACTGGGT CCGGTCCATA ATCATGTTAC 
     3781 AGGAAAAGGC CCAGGACAGG GAACGGCTGC TCTTGAAGTT CATCAAGATC ATGAAGCACT 
     3841 TGCGGAAGCT GAATAACTTC AACTCCTACT TGGCCATCCT CTCTGCCCTG GACTCGGCGC 
     3901 CCATCCGCAG GCTGGAGTGG CAGAAGCAGA CTTCAGAGGG CCTGGCCGAG TACTGCACAC 
     3961 TGATCGACAG CTCGTCCTCC TTCCGAGCCT ACCGGGCCGC CCTCTCGGAG GTGGAACCGC 
     4021 CGTGCATCCC GTACCTGGGG CTGATCCTGC AGGACCTGAC CTTCGTTCAC CTGGGAAACC 
     4081 CAGACTACAT CGACGGGAAA GTGAACTTCT CCAAGCGGTG GCAGCAGTTC AACATCCTCG 
     4141 ACAGCATGCG CTGCTTCCAG CAGGCGCACT ATGACATGCG GAGGAACGAC GACATTATAA 
     4201 ACTTCTTCAA TGACTTCAGT GACCACCTGG CTGAGGAGGC CCTATGGGAA CTGTCTCTGA 
     4261 AAATTAAACC CAGGAACATA ACAAGGAGAA AAACAGACCG GGAAGAGAAG ACCACGCGTA 
     4321 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4381 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4441 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4501 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4561 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4621 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4681 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4741 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4801 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     4861 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     4921 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     4981 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5041 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5101 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5161 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5221 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5281 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5341 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5401 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5461 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5521 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5581 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5641 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5701 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     5761 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     5821 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     5881 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     5941 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6001 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6061 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6121 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6181 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6241 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6301 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6361 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6421 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6481 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6541 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6601 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6661 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6721 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     6781 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     6841 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     6901 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     6961 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7021 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7081 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7141 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7201 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7261 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7321 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7381 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7441 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7501 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7561 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7621 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7681 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7741 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     7801 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     7861 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     7921 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     7981 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8041 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8101 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8161 AATTTT
//