LOCUS       RC208049                7932 bp    DNA     circular  SYN 
DEFINITION  HIRA (Myc-DDK-tagged)-Human HIR histone cell cycle regulation defective homolog A (S. cerevisiae) (HIRA)
ACCESSION   NM_003325
VERSION     NM_003325.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4089..4180 
                     /label="Myc-DDK"  
     misc_feature    4210..4795 
                     /label="PolyA signal"  
     misc_feature    4267..4286 
                     /label="Restriction Enzyme"  
     misc_feature    5071..5743 
                     /label="ColE1"  
     misc_feature    6173..6967 
                     /label="Kan/Neo"  
     misc_feature    7002..7304 
                     /label="SV40 ori"  
     misc_feature    7390..7867 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4071..4077 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4071 
                     /label="HIRA"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGCTCCTG AAGCCGACCT GGGTCAACCA CAATGGCAAG CCGATTTTTT 
     1081 CAGTTGATAT TCACCCTGAC GGGACCAAGT TCGCAACTGG AGGACAAGGG CAGGATTCTG 
     1141 GGAAGGTTGT GATCTGGAAT ATGTCTCCAG TCCTCCAGGA GGATGACGAG AAGGATGAAA 
     1201 ATATTCCCAA GATGCTTTGC CAGATGGACA ATCACTTAGC ATGTGTGAAC TGTGTGCGGT 
     1261 GGTCAAACAG TGGGATGTAT TTAGCTTCTG GGGGAGATGA CAAACTGATT ATGGTGTGGA 
     1321 AGCGGGCTAC GTACATCGGC CCCAGCACCG TGTTCGGCTC CAGTGGTAAG CTTGCCAATG 
     1381 TGGAGCAGTG GCGGTGTGTC TCTATCCTCC GGAATCATTC AGGCGATGTG ATGGATGTAG 
     1441 CATGGTCTCC CCACGATGCC TGGCTAGCCT CATGCAGCGT GGATAACACT GTCGTCATCT 
     1501 GGAATGCTGT AAAGTTCCCA GAAATTCTAG CTACTCTGAG AGGTCATTCT GGCTTGGTCA 
     1561 AAGGGTTGAC ATGGGACCCT GTTGGTAAAT ACATAGCTTC TCAAGCTGAT GACCGCAGCC 
     1621 TAAAGGTGTG GAGGACGCTG GACTGGCAGT TGGAGACCAG CATCACCAAG CCTTTTGATG 
     1681 AGTGTGGAGG AACGACCCAT GTGTTGCGGC TCAGCTGGTC ACCTGATGGG CATTACCTGG 
     1741 TGTCTGCCCA TGCCATGAAC AACTCAGGCC CCACTGCCCA GATCATCGAA CGGGAGGGAT 
     1801 GGAAGACCAA CATGGACTTT GTTGGGCACC GGAAAGCTGT GACTGTCGTG AAATTCAACC 
     1861 CAAAAATCTT CAAAAAGAAG CAGAAGAATG GGAGTTCTGC GAAGCCTAGC TGCCCGTACT 
     1921 GCTGCTGTGC TGTTGGCAGC AAGGACCGCT CGCTTTCTGT CTGGCTCACA TGTCTGAAAC 
     1981 GGCCGCTGGT GGTCATCCAT GAACTGTTTG ACAAATCCAT CATGGATATT TCCTGGACTC 
     2041 TGAATGGGCT GGGCATCTTG GTATGCTCTA TGGACGGCTC TGTGGCATTC CTCGACTTCT 
     2101 CCCAGGATGA GCTTGGCGAT CCCCTGAGCG AGGAGGAGAA GAGCCGCATT CACCAGTCCA 
     2161 CCTATGGCAA GAGCCTAGCC ATCATGACCG AGGCCCAGCT CTCCACAGCC GTCATTGAGA 
     2221 ACCCTGAGAT GCTCAAGTAC CAGCGAAGGC AGCAGCAGCA GCAGCTGGAC CAGAAGAGTG 
     2281 CTGCGACCAG GGAGATGGGC TCAGCCACCT CAGTCGCAGG CGTTGTCAAC GGGGAGAGTC 
     2341 TTGAAGATAT CAGGAAGAAT CTTTTGAAGA AACAAGTTGA GACTCGGACA GCAGATGGCC 
     2401 GGAGAAGAAT CACGCCTCTC TGCATAGCAC AGCTGGACAC TGGGGACTTC TCCACGGCAT 
     2461 TCTTTAACAG CATCCCCCTC TCGGGCTCCC TGGCGGGCAC CATGCTCTCT TCTCATAGCA 
     2521 GTCCACAGCT ACTGCCACTG GACTCCAGTA CCCCTAACTC CTTCGGCGCC TCGAAGCCTT 
     2581 GCACAGAGCC TGTGGTGGCT GCCAGTGCCA GACCTGCAGG CGATTCTGTC AATAAAGACA 
     2641 GTATGAATGC TACCTCTACT CCTGCTGCAT TGTCACCTTC TGTGTTAACG ACCCCGTCCA 
     2701 AGATCGAACC CATGAAAGCG TTTGACTCCC GGTTCACAGA GCGGTCCAAA GCCACACCAG 
     2761 GTGCTCCTGC CCTGACCAGC ATGACTCCGA CAGCTGTGGA AAGGTTAAAA GAGCAGAACC 
     2821 TTGTGAAAGA GCTGAGGCCC CGAGACCTCC TGGAGAGCAG CAGTGACAGC GATGAGAAAG 
     2881 TCCCTTTGGC TAAGGCTTCC TCACTGTCCA AGCGAAAACT TGAGCTTGAG GTAGAGACAG 
     2941 TAGAGAAGAA GAAGAAAGGG CGGCCTCGGA AGGACTCTCG TCTCATGCCT GTGTCTCTGT 
     3001 CTGTCCAGTC TCCAGCTGCC CTAACCGCAG AGAAGGAGGC CATGTGTCTG TCTGCACCAG 
     3061 CACTTGCACT GAAGCTGCCA ATTCCAAGCC CCCAGAGAGC ATTCACCCTC CAGGTCAGCT 
     3121 CCGATCCTTC CATGTACATT GAGGTGGAGA ATGAAGTGAC AGTGGTGGGG GGCGTGAAGC 
     3181 TGAGCCGCCT GAAGTGCAAC CGGGAAGGGA AGGAGTGGGA GACGGTACTC ACCAGCCGGA 
     3241 TCCTCACTGC TGCGGGCAGC TGTGACGTGG TGTGTGTCGC CTGTGAAAAA AGGATGCTGT 
     3301 CAGTGTTCTC CACCTGTGGT CGCCGTCTCC TCTCTCCCAT CCTCCTGCCA TCCCCGATCT 
     3361 CTACTTTGCA TTGCACAGGC TCCTACGTCA TGGCGCTCAC CGCTGCAGCC ACACTCTCTG 
     3421 TCTGGGATGT TCACAGACAG GTGGTTGTGG TGAAAGAAGA GTCTCTACAC TCCATCCTGG 
     3481 CAGGAAGTGA TATGACGGTA TCACAGATCT TGCTGACGCA GCATGGAATC CCAGTAATGA 
     3541 ACCTGTCCGA TGGGAAGGCG TACTGCTTTA ATCCGTCACT TTCCACATGG AACCTGGTTT 
     3601 CTGACAAGCA GGACTCACTG GCTCAGTGTG CAGACTTTAG GAGCAGCCTG CCATCCCAGG 
     3661 ACGCCATGCT GTGCTCAGGA CCGTTAGCCA TAATCCAGGG CCGCACCTCC AACTCGGGAA 
     3721 GGCAGGCTGC CCGGCTCTTC TCCGTGCCTC ATGTGGTGCA GCAAGAGACC ACCCTGGCCT 
     3781 ACCTAGAGAA CCAGGTGGCA GCAGCACTCA CCCTGCAGTC CAGCCACGAG TACCGCCATT 
     3841 GGCTCCTCGT CTACGCACGG TACCTCGTAA ACGAAGGGTT TGAATACCGA CTTCGAGAAA 
     3901 TATGCAAGGA CTTACTGGGT CCGGTTCACT ACTCCACTGG AAGCCAGTGG GAGTCAACAG 
     3961 TAGTGGGTCT GCGGAAGAGG GAGCTGCTGA AGGAGCTGCT ACCAGTCATC GGGCAGAACC 
     4021 TCCGATTCCA GCGCCTCTTC ACCGAGTGTC AGGAACAGCT CGACATCCTG AGGGACAAGA 
     4081 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4141 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4201 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4261 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4321 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4381 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4441 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4501 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4561 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4621 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     4681 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     4741 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     4801 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     4861 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     4921 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     4981 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5041 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5101 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5161 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5221 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5281 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5341 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     5401 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     5461 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     5521 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     5581 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     5641 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     5701 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     5761 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     5821 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     5881 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     5941 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6001 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6061 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6121 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6181 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6241 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6301 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6361 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     6421 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     6481 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     6541 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     6601 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     6661 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     6721 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     6781 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     6841 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     6901 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     6961 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7021 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7081 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7141 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7201 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7261 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7321 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7381 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     7441 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     7501 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     7561 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     7621 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     7681 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     7741 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     7801 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     7861 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     7921 AACGCGAATT TT
//