LOCUS       RC207978                8829 bp    DNA     circular  SYN 
DEFINITION  TTC21B (Myc-DDK-tagged)-Human tetratricopeptide repeat domain 21B (TTC21B)
ACCESSION   NM_024753
VERSION     NM_024753.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4986..5077 
                     /label="Myc-DDK"  
     misc_feature    5107..5692 
                     /label="PolyA signal"  
     misc_feature    5164..5183 
                     /label="Restriction Enzyme"  
     misc_feature    5968..6640 
                     /label="ColE1"  
     misc_feature    7070..7864 
                     /label="Kan/Neo"  
     misc_feature    7899..8201 
                     /label="SV40 ori"  
     misc_feature    8287..8764 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4968..4974 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4968 
                     /label="TTC21B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACTCGCAG GAATTGAAGA CTTTGATTAA TTACTATTGT CAAGAGAGAT 
     1081 ATTTCCATCA TGTATTACTG GTTGCCAGTG AAGGAATTAA GAGGTATGGA AGTGATCCAG 
     1141 TCTTCAGGTT TTATCATGCC TATGGCACAT TAATGGAAGG TAAAACTCAA GAAGCTCTTC 
     1201 GAGAATTTGA GGCTATTAAA AATAAACAAG ATGTATCACT TTGTTCTCTA CTTGCACTGA 
     1261 TATATGCCCA TAAAATGAGT CCTAATCCAG ATAGAGAAGC TATTCTGGAA TCAGATGCCA 
     1321 GAGTGAAGGA ACAACGTAAA GGAGCTGGAG AGAAAGCCTT ATACCATGCA GGCTTATTTT 
     1381 TATGGCACAT TGGTCGCCAT GATAAAGCAA GGGAATATAT TGACAGAATG ATCAAAATAT 
     1441 CAGATGGTAG TAAACAGGGA CACGTTTTGA AAGCATGGCT TGATATTACA AGAGGAAAAG 
     1501 AGCCTTACAC TAAAAAAGCA CTGAAGTATT TTGAAGAGGG ACTCCAAGAT GGGAATGATA 
     1561 CTTTTGCTCT GCTGGGTAAG GCACAATGCC TTGAGATGCG CCAGAATTAT TCAGGTGCCC 
     1621 TGGAGACTAT GAACCAGATA ATCGTGAATT TTCCGAGCTT CCTTCCTGCT TTTGTTAAGA 
     1681 AAATGAAATT ACAACTAGCC TTGCAGGATT GGGACCAGAC AGTTGAGACA GCACAAAGGT 
     1741 TGCTGCTCCA AGATAGCCAA AATGTGGAAG CACTGAGAAT GCAGGCACTC TACTATGTGT 
     1801 GTAGAGAGGG GGATATAGAG AAGGCTTCCA CCAAGCTGGA AAACTTGGGA AATGCATTGG 
     1861 ATGCCATGGA ACCACAGAAT GCTCAACTTT TCTATAACAT TACACTCGCC TTCAGCAGAA 
     1921 CTTGTGGACG TAGTCAACTT ATTCTTCAAA AAATTCAAAC GTTACTTGAG AGAGCTTTTA 
     1981 GTTTAAACCC TCAGCAATCA GAATTTGCTA CAGAACTTGG ATACCAAATG ATTTTACAAG 
     2041 GAAGAGTTAA AGAGGCACTG AAGTGGTATA AGACCGCCAT GACACTTGAT GAGACTAGTG 
     2101 TGTCTGCCCT AGTTGGATTT ATCCAATGTC AGTTGATAGA AGGGCAATTA CAGGATGCAG 
     2161 ATCAGCAGCT AGAATTTTTA AATGAAATCC AGCAATCCAT TGGAAAATCT GCGGAATTAA 
     2221 TCTATTTACA TGCAGTTCTT GCCATGAAGA AAAATAAACG ACAAGAAGAA GTTATTAATT 
     2281 TGTTAAATGA TGTCCTGGAC ACTCACTTTT CACAATTAGA AGGTTTGCCT CTTGGCATAC 
     2341 AGTATTTTGA AAAGCTAAAT CCTGATTTCT TGTTAGAAAT TGTTATGGAG TATCTGAGCT 
     2401 TCTGTCCAAT GCAGCCTGCA AGTCCTGGGC AACCTCTTTG TCCATTTCTC AGGCGTTGCA 
     2461 TCTCAGTCCT GGAGACTGTA GTAAGAACTG TTCCAGGTCT TCTGCAAACA GTCTTCCTAA 
     2521 TAGCAAAAGT GAAATATTTG TCAGGTGATA TTGAAGCAGC TTTCAATAAC CTTCAGCACT 
     2581 GCTTAGAACA CAATCCCTCT TATGCTGATG CTCATCTGCT GCTAGCTCAG GTTTACTTGT 
     2641 CTCAAGAAAA AGTCAAATTG TGTTCTCAGT CTCTTGAACT TTGTCTGAGC TATGATTTTA 
     2701 AGGTGAGAGA CTATCCTTTA TACCATTTGA TAAAAGCTCA GTCACAAAAG AAAATGGGAG 
     2761 AAATAGCAGA CGCAATTAAA ACACTGCATA TGGCAATGAG TTTACCAGGA ATGAAAAGAA 
     2821 TTGGAGCTTC CACAAAATCA AAAGACAGAA AAACTGAAGT TGATACAAGC CATCGTTTAT 
     2881 CGATCTTTCT TGAATTGATA GACGTTCACC GCTTAAATGG AGAGCAGCAT GAGGCAACCA 
     2941 AAGTTTTACA AGATGCCATC CATGAATTTT CTGGAACATC TGAAGAAGTG CGGGTTACCA 
     3001 TTGCTAATGC AGACCTTGCT CTAGCCCAAG GAGATATTGA ACGGGCTTTA AGCATCCTTC 
     3061 AGAATGTTAC AGCCGAACAG CCTTATTTTA TAGAGGCCAG AGAAAAAATG GCAGATATTT 
     3121 ATCTGAAGCA CAGAAAAGAT AAAATGTTAT ATATCACTTG TTTTAGAGAA ATTGCTGAAA 
     3181 GAATGGCTAA CCCTCGGTCT TTTCTTCTCC TTGGTGATGC ATACATGAAT ATTCTAGAGC 
     3241 CTGAAGAAGC CATAGTAGCA TATGAGCAAG CATTAAATCA GAACCCGAAA GATGGAACAT 
     3301 TGGCAAGCAA AATGGGCAAA GCACTTATCA AAACTCATAA CTACTCAATG GCAATCACTT 
     3361 ACTATGAAGC TGCTCTGAAA ACTGGACAAA AGAATTATCT TTGCTATGAC CTCGCTGAGC 
     3421 TCTTATTAAA ATTGAAATGG TATGACAAAG CAGAAAAAGT TCTTCAGCAT GCTCTGGCTC 
     3481 ATGAACCTGT AAATGAACTG TCAGCTCTCA TGGAGGATGG ACGTTGTCAA GTTCTTCTAG 
     3541 CAAAAGTTTA TAGTAAAATG GAAAAACTTG GTGATGCGAT CACTGCATTA CAACAGGCTC 
     3601 GAGAATTACA AGCTCGGGTA CTAAAACGTG TTCAGATGGA ACAGCCAGAT GCAGTTCCTG 
     3661 CACAGAAACA TTTAGCAGCT GAAATTTGTG CAGAGATTGC AAAACATTCT GTTGCTCAGC 
     3721 GAGACTATGA AAAAGCAATT AAGTTTTATA GAGAGGCTCT GGTTCACTGC GAAACAGATA 
     3781 ATAAGATTAT GTTGGAACTG GCACGATTAT ACCTGGCACA AGATGACCCT GATTCCTGCC 
     3841 TGCGGCAGTG TGCTCTACTG CTTCAGAGTG ACCAGGATAA CGAAGCTGCT ACCATGATGA 
     3901 TGGCTGATCT CATGTTCAGA AAACAAGACT ATGAACAAGC AGTGTTTCAT TTACAGCAGC 
     3961 TTTTAGAACG TAAGCCAGAT AATTATATGA CATTATCTCG TTTGATTGAT CTCCTAAGAA 
     4021 GATGTGGAAA ACTCGAGGAT GTCCCAAGAT TTTTCTCAAT GGCTGAGAAA CGTAACTCCA 
     4081 GAGCAAAATT GGAACCAGGA TTTCAGTATT GTAAAGGACT GTATCTTTGG TACACTGGAG 
     4141 AACCAAATGA TGCCCTTCGA CATTTTAATA AAGCTCGGAA AGATCGTGAC TGGGGCCAAA 
     4201 ATGCCCTTTA TAATATGATA GAGATCTGTT TGAATCCAGA TAATGAAACT GTTGGAGGTG 
     4261 AAGTATTTGA AAACCTGGAT GGAGACCTGG GTAATTCAAC TGAGAAGCAA GAATCTGTGC 
     4321 AACTGGCAGT AAGAACAGCA GAAAAACTTC TTAAGGAACT AAAACCTCAG ACTGTTCAGG 
     4381 GTCACGTACA GCTTCGCATA ATGGAAAACT ATTGCTTAAT GGCTACCAAA CAGAAATCTA 
     4441 ATGTTGAACA AGCATTAAAT ACCTTCACTG AAATAGCAGC ATCTGAGAAG GAGCATATCC 
     4501 CAGCGCTCTT GGGAATGGCA ACGGCTTATA TGATCTTGAA ACAGACTCCA CGAGCCAGAA 
     4561 ACCAGCTGAA GCGTATTGCG AAAATGAATT GGAATGCTAT TGATGCTGAA GAGTTTGAGA 
     4621 AGAGTTGGCT GCTACTTGCT GATATTTACA TTCAATCAGC AAAATATGAC ATGGCAGAAG 
     4681 ACCTGTTAAA ACGGTGCCTG CGTCATAATA GATCTTGCTG CAAAGCTTAT GAATATATGG 
     4741 GATACATTAT GGAAAAAGAG CAAGCATATA CAGATGCTGC CTTGAACTAT GAGATGGCAT 
     4801 GGAAATATAG CAATCGGACA AATCCGGCAG TAGGATACAA ACTGGCATTT AATTACTTAA 
     4861 AAGCAAAAAG ATATGTGGAT TCAATTGACA TATGTCACCA GGTTCTTGAA GCACATCCAA 
     4921 CTTATCCAAA AATCAGAAAG GATATACTTG ATAAGGCCCG TGCGTCTTTA AGACCTACGC 
     4981 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     5041 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     5101 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     5161 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5221 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5281 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5341 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5401 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5461 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5521 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5581 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5641 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5701 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5761 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5821 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5881 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5941 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     6001 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     6061 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     6121 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6181 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6241 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6301 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6361 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6421 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6481 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6541 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6601 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6661 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6721 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6781 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6841 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6901 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6961 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     7021 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     7081 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     7141 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7201 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7261 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7321 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7381 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7441 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7501 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7561 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7621 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7681 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7741 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7801 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7861 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7921 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7981 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     8041 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     8101 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     8161 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8221 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8281 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8341 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8401 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8461 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8521 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8581 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8641 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8701 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8761 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8821 GCGAATTTT
//